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P70665 (SIAE_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialate O-acetylesterase

EC=3.1.1.53
Alternative name(s):
Sialic acid-specific 9-O-acetylesterase
Yolk sac protein 2
Gene names
Name:Siae
Synonyms:Ysg2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length541 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid.

Catalytic activity

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Enzyme regulation

Inhibited by diisopropyl fluorophosphate and diethyl-P-nitrophenyl phosphate By similarity.

Subunit structure

Disulfide-linked heterodimer of a small subunit and a large subunit By similarity.

Subcellular location

Isoform 1: Lysosome.

Isoform 2: Cytoplasm.

Tissue specificity

Isoform 1 is widely expressed. Isoform 2 shows a more restricted distribution with highest expression in brain and ovary and lower levels in liver and thymus.

Post-translational modification

The two subunits are derived from a single precursor by proteolytic cleavage By similarity.

The lysosomal isoform isglycosylated.

Sequence caution

The sequence BAC29164.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
Lysosome
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionHydrolase
Serine esterase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsialate O-acetylesterase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P70665-1)

Also known as: Lse;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P70665-2)

Also known as: Cse;

The sequence of this isoform differs from the canonical sequence as follows:
     1-97: Missing.
Isoform 3 (identifier: P70665-3)

The sequence of this isoform differs from the canonical sequence as follows:
     349-390: LSSYMLKNSS...MPNTFMAVAI → VCIQRIHIQC...NICPNSMSWR
     391-541: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 By similarity
Chain24 – 275252Sialate O-acetylesterase small subunit
PRO_0000022714
Chain276 – 541266Sialate O-acetylesterase large subunit
PRO_0000022715

Amino acid modifications

Glycosylation1071N-linked (GlcNAc...) Potential
Glycosylation1381N-linked (GlcNAc...) Potential
Glycosylation1881N-linked (GlcNAc...) Potential
Glycosylation2931N-linked (GlcNAc...) Potential
Glycosylation3561N-linked (GlcNAc...) Potential
Glycosylation4271N-linked (GlcNAc...) Potential
Glycosylation4481N-linked (GlcNAc...) Potential
Glycosylation4621N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 9797Missing in isoform 2.
VSP_004077
Alternative sequence349 – 39042LSSYM…MAVAI → VCIQRIHIQCLEFMGLCGEC GLCTCLYWDLQPNICPNSMS WR in isoform 3.
VSP_018996
Alternative sequence391 – 541151Missing in isoform 3.
VSP_018997

Experimental info

Sequence conflict1141D → N in BAE37942. Ref.4
Sequence conflict1601I → T in AAB07813. Ref.2
Sequence conflict1601I → T in AAH07136. Ref.5
Sequence conflict3491L → Q in BAC26026. Ref.4
Sequence conflict3731F → L in BAC29164. Ref.4
Sequence conflict3791P → L in AAH07136. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Lse) [UniParc].

Last modified May 30, 2006. Version 3.
Checksum: 944936C45C4A2E6B

FASTA54160,775
        10         20         30         40         50         60 
MVSPGPVFGI VLLIIARVSR SAGIGFRFAS YIDNYMVLQK EPSGAVIWGF GTPGATVTVT 

        70         80         90        100        110        120 
LCQGQETIMK KVTSVKEPSN TWMVVLDPMK PGGPFEVMAQ QTLGTMNFTL RVHDVLFGDV 

       130        140        150        160        170        180 
WLCSGQSNMQ MTVSQIFNAS KELSDTAAYQ SVRIFSVSLI QSEEELDDLT EVDLSWSKPT 

       190        200        210        220        230        240 
AGNLGHGNFT YMSAVCWLFG RYLYDTLQYP IGLVSSSWGG TYIEVWSSRR TLKACGVPNT 

       250        260        270        280        290        300 
RDERVGQPEI KPMRNECNSE ESSCPFRVVP SVRVTGPTRH SVLWNAMIHP LQNMTLKGVV 

       310        320        330        340        350        360 
WYQGESNADY NRDLYTCMFP ELIEDWRQTF HYGSQGQTDR FFPFGFVQLS SYMLKNSSDY 

       370        380        390        400        410        420 
GFPEIRWHQT ADFGHVPNPK MPNTFMAVAI DLCDRDSPFG SIHPRDKQTV AYRLHLGARA 

       430        440        450        460        470        480 
VAYGEKNLTF QGPLPKKIEL LASNGLLNLT YDQEIQVQMQ DNKTFEISCC SDRHCKWLPA 

       490        500        510        520        530        540 
PVNTFSTQTL ILDLNACLGT VVAVRYAWTT WPCEYKQCAV YHTSSMLPAP PFIAQISHRG 


I 

« Hide

Isoform 2 (Cse) [UniParc].

Checksum: 0AFDFCCEF3549C53
Show »

FASTA44450,389
Isoform 3 [UniParc].

Checksum: 7EED447751832279
Show »

FASTA39043,888

References

« Hide 'large scale' references
[1]"Molecular cloning of the cDNA encoding a murine sialic acid-specific 9-O-acetylesterase and RNA expression in cells of hematopoietic and non-hematopoietic origin."
Stoddart A., Zhang Y., Paige C.J.
Nucleic Acids Res. 24:4003-4008(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: C57BL/6 X DBA/2.
[2]"Molecular cloning and characterization of lysosomal sialic acid O-acetylesterase."
Guimaraes M.J., Bazan J.F., Castagnola J., Diaz S., Copeland N.G., Gilbert D.J., Jenkins N.A., Varki A., Zlotnik A.
J. Biol. Chem. 271:13697-13705(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Lysosomal and cytosolic sialic acid 9-O-acetylesterase activities can be encoded by one gene via differential usage of a signal peptide-encoding exon at the N-terminus."
Takematsu H., Diaz S., Stoddart A., Zhang Y., Varki A.
J. Biol. Chem. 274:25623-25631(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: B-cell.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Strain: C57BL/6J.
Tissue: Blastocyst, Cerebellum, Diencephalon, Embryonic head, Skin and Urinary bladder.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U61183 mRNA. Translation: AAC52880.1.
X98625 mRNA. Translation: CAA67214.1.
U40408 mRNA. Translation: AAB07813.1.
AF156856 mRNA. Translation: AAD55976.1.
AK028598 mRNA. Translation: BAC26026.1.
AK028656 mRNA. Translation: BAC26049.1.
AK033980 mRNA. Translation: BAC28536.1.
AK035715 mRNA. Translation: BAC29164.1. Different initiation.
AK043392 mRNA. Translation: BAC31534.1.
AK164852 mRNA. Translation: BAE37942.1.
AK167103 mRNA. Translation: BAE39252.1.
BC007136 mRNA. Translation: AAH07136.1.
CCDSCCDS22983.1. [P70665-1]
RefSeqNP_035864.2. NM_011734.3. [P70665-1]
UniGeneMm.196345.

3D structure databases

ProteinModelPortalP70665.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP70665. 1 interaction.
MINTMINT-4115423.
STRING10090.ENSMUSP00000002007.

PTM databases

PhosphoSiteP70665.

Proteomic databases

PaxDbP70665.
PRIDEP70665.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000002007; ENSMUSP00000002007; ENSMUSG00000001942. [P70665-1]
GeneID22619.
KEGGmmu:22619.
UCSCuc009ove.1. mouse. [P70665-3]
uc009ovf.1. mouse. [P70665-1]

Organism-specific databases

CTD54414.
MGIMGI:104803. Siae.

Phylogenomic databases

eggNOGNOG41492.
GeneTreeENSGT00390000010608.
HOVERGENHBG007955.
InParanoidP70665.
KOK05970.
OMALRFASYY.
OrthoDBEOG7H1JKC.
PhylomeDBP70665.
TreeFamTF328611.

Gene expression databases

ArrayExpressP70665.
BgeeP70665.
CleanExMM_SIAE.
GenevestigatorP70665.

Family and domain databases

InterProIPR005181. DUF303_acetylest.
[Graphical view]
PfamPF03629. DUF303. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302973.
PROP70665.
SOURCESearch...

Entry information

Entry nameSIAE_MOUSE
AccessionPrimary (citable) accession number: P70665
Secondary accession number(s): Q3TNZ5 expand/collapse secondary AC list , Q544V7, Q61044, Q8C902, Q8CBM6, Q8CC41, Q8CEB7, Q922L0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 30, 2006
Last modified: July 9, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot