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P70665

- SIAE_MOUSE

UniProt

P70665 - SIAE_MOUSE

Protein

Sialate O-acetylesterase

Gene

Siae

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 3 (30 May 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid.

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Enzyme regulationi

    Inhibited by diisopropyl fluorophosphate and diethyl-P-nitrophenyl phosphate.By similarity

    GO - Molecular functioni

    1. sialate O-acetylesterase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sialate O-acetylesterase (EC:3.1.1.53)
    Alternative name(s):
    Sialic acid-specific 9-O-acetylesterase
    Yolk sac protein 2
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Siae
    Synonyms:Ysg2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:104803. Siae.

    Subcellular locationi

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323By similarityAdd
    BLAST
    Chaini24 – 275252Sialate O-acetylesterase small subunitPRO_0000022714Add
    BLAST
    Chaini276 – 541266Sialate O-acetylesterase large subunitPRO_0000022715Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi427 – 4271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi448 – 4481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi462 – 4621N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The two subunits are derived from a single precursor by proteolytic cleavage.By similarity
    The lysosomal isoform is glycosylated.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP70665.
    PRIDEiP70665.

    PTM databases

    PhosphoSiteiP70665.

    Expressioni

    Tissue specificityi

    Isoform 1 is widely expressed. Isoform 2 shows a more restricted distribution with highest expression in brain and ovary and lower levels in liver and thymus.

    Gene expression databases

    ArrayExpressiP70665.
    BgeeiP70665.
    CleanExiMM_SIAE.
    GenevestigatoriP70665.

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer of a small subunit and a large subunit.By similarity

    Protein-protein interaction databases

    IntActiP70665. 1 interaction.
    MINTiMINT-4115423.
    STRINGi10090.ENSMUSP00000002007.

    Structurei

    3D structure databases

    ProteinModelPortaliP70665.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG41492.
    GeneTreeiENSGT00390000010608.
    HOVERGENiHBG007955.
    InParanoidiP70665.
    KOiK05970.
    OMAiLRFASYY.
    OrthoDBiEOG7H1JKC.
    PhylomeDBiP70665.
    TreeFamiTF328611.

    Family and domain databases

    InterProiIPR005181. DUF303_acetylest.
    [Graphical view]
    PfamiPF03629. DUF303. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P70665-1) [UniParc]FASTAAdd to Basket

    Also known as: Lse

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVSPGPVFGI VLLIIARVSR SAGIGFRFAS YIDNYMVLQK EPSGAVIWGF    50
    GTPGATVTVT LCQGQETIMK KVTSVKEPSN TWMVVLDPMK PGGPFEVMAQ 100
    QTLGTMNFTL RVHDVLFGDV WLCSGQSNMQ MTVSQIFNAS KELSDTAAYQ 150
    SVRIFSVSLI QSEEELDDLT EVDLSWSKPT AGNLGHGNFT YMSAVCWLFG 200
    RYLYDTLQYP IGLVSSSWGG TYIEVWSSRR TLKACGVPNT RDERVGQPEI 250
    KPMRNECNSE ESSCPFRVVP SVRVTGPTRH SVLWNAMIHP LQNMTLKGVV 300
    WYQGESNADY NRDLYTCMFP ELIEDWRQTF HYGSQGQTDR FFPFGFVQLS 350
    SYMLKNSSDY GFPEIRWHQT ADFGHVPNPK MPNTFMAVAI DLCDRDSPFG 400
    SIHPRDKQTV AYRLHLGARA VAYGEKNLTF QGPLPKKIEL LASNGLLNLT 450
    YDQEIQVQMQ DNKTFEISCC SDRHCKWLPA PVNTFSTQTL ILDLNACLGT 500
    VVAVRYAWTT WPCEYKQCAV YHTSSMLPAP PFIAQISHRG I 541
    Length:541
    Mass (Da):60,775
    Last modified:May 30, 2006 - v3
    Checksum:i944936C45C4A2E6B
    GO
    Isoform 2 (identifier: P70665-2) [UniParc]FASTAAdd to Basket

    Also known as: Cse

    The sequence of this isoform differs from the canonical sequence as follows:
         1-97: Missing.

    Show »
    Length:444
    Mass (Da):50,389
    Checksum:i0AFDFCCEF3549C53
    GO
    Isoform 3 (identifier: P70665-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         349-390: LSSYMLKNSS...MPNTFMAVAI → VCIQRIHIQC...NICPNSMSWR
         391-541: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:390
    Mass (Da):43,888
    Checksum:i7EED447751832279
    GO

    Sequence cautioni

    The sequence BAC29164.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti114 – 1141D → N in BAE37942. (PubMed:16141072)Curated
    Sequence conflicti160 – 1601I → T in AAB07813. (PubMed:8662838)Curated
    Sequence conflicti160 – 1601I → T in AAH07136. (PubMed:15489334)Curated
    Sequence conflicti349 – 3491L → Q in BAC26026. (PubMed:16141072)Curated
    Sequence conflicti373 – 3731F → L in BAC29164. (PubMed:16141072)Curated
    Sequence conflicti379 – 3791P → L in AAH07136. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 9797Missing in isoform 2. 1 PublicationVSP_004077Add
    BLAST
    Alternative sequencei349 – 39042LSSYM…MAVAI → VCIQRIHIQCLEFMGLCGEC GLCTCLYWDLQPNICPNSMS WR in isoform 3. 1 PublicationVSP_018996Add
    BLAST
    Alternative sequencei391 – 541151Missing in isoform 3. 1 PublicationVSP_018997Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U61183 mRNA. Translation: AAC52880.1.
    X98625 mRNA. Translation: CAA67214.1.
    U40408 mRNA. Translation: AAB07813.1.
    AF156856 mRNA. Translation: AAD55976.1.
    AK028598 mRNA. Translation: BAC26026.1.
    AK028656 mRNA. Translation: BAC26049.1.
    AK033980 mRNA. Translation: BAC28536.1.
    AK035715 mRNA. Translation: BAC29164.1. Different initiation.
    AK043392 mRNA. Translation: BAC31534.1.
    AK164852 mRNA. Translation: BAE37942.1.
    AK167103 mRNA. Translation: BAE39252.1.
    BC007136 mRNA. Translation: AAH07136.1.
    CCDSiCCDS22983.1. [P70665-1]
    RefSeqiNP_035864.2. NM_011734.3. [P70665-1]
    UniGeneiMm.196345.

    Genome annotation databases

    EnsembliENSMUST00000002007; ENSMUSP00000002007; ENSMUSG00000001942. [P70665-1]
    GeneIDi22619.
    KEGGimmu:22619.
    UCSCiuc009ove.1. mouse. [P70665-3]
    uc009ovf.1. mouse. [P70665-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U61183 mRNA. Translation: AAC52880.1 .
    X98625 mRNA. Translation: CAA67214.1 .
    U40408 mRNA. Translation: AAB07813.1 .
    AF156856 mRNA. Translation: AAD55976.1 .
    AK028598 mRNA. Translation: BAC26026.1 .
    AK028656 mRNA. Translation: BAC26049.1 .
    AK033980 mRNA. Translation: BAC28536.1 .
    AK035715 mRNA. Translation: BAC29164.1 . Different initiation.
    AK043392 mRNA. Translation: BAC31534.1 .
    AK164852 mRNA. Translation: BAE37942.1 .
    AK167103 mRNA. Translation: BAE39252.1 .
    BC007136 mRNA. Translation: AAH07136.1 .
    CCDSi CCDS22983.1. [P70665-1 ]
    RefSeqi NP_035864.2. NM_011734.3. [P70665-1 ]
    UniGenei Mm.196345.

    3D structure databases

    ProteinModelPortali P70665.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P70665. 1 interaction.
    MINTi MINT-4115423.
    STRINGi 10090.ENSMUSP00000002007.

    PTM databases

    PhosphoSitei P70665.

    Proteomic databases

    PaxDbi P70665.
    PRIDEi P70665.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002007 ; ENSMUSP00000002007 ; ENSMUSG00000001942 . [P70665-1 ]
    GeneIDi 22619.
    KEGGi mmu:22619.
    UCSCi uc009ove.1. mouse. [P70665-3 ]
    uc009ovf.1. mouse. [P70665-1 ]

    Organism-specific databases

    CTDi 54414.
    MGIi MGI:104803. Siae.

    Phylogenomic databases

    eggNOGi NOG41492.
    GeneTreei ENSGT00390000010608.
    HOVERGENi HBG007955.
    InParanoidi P70665.
    KOi K05970.
    OMAi LRFASYY.
    OrthoDBi EOG7H1JKC.
    PhylomeDBi P70665.
    TreeFami TF328611.

    Miscellaneous databases

    NextBioi 302973.
    PROi P70665.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70665.
    Bgeei P70665.
    CleanExi MM_SIAE.
    Genevestigatori P70665.

    Family and domain databases

    InterProi IPR005181. DUF303_acetylest.
    [Graphical view ]
    Pfami PF03629. DUF303. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the cDNA encoding a murine sialic acid-specific 9-O-acetylesterase and RNA expression in cells of hematopoietic and non-hematopoietic origin."
      Stoddart A., Zhang Y., Paige C.J.
      Nucleic Acids Res. 24:4003-4008(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: C57BL/6 X DBA/2.
    2. "Molecular cloning and characterization of lysosomal sialic acid O-acetylesterase."
      Guimaraes M.J., Bazan J.F., Castagnola J., Diaz S., Copeland N.G., Gilbert D.J., Jenkins N.A., Varki A., Zlotnik A.
      J. Biol. Chem. 271:13697-13705(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Lysosomal and cytosolic sialic acid 9-O-acetylesterase activities can be encoded by one gene via differential usage of a signal peptide-encoding exon at the N-terminus."
      Takematsu H., Diaz S., Stoddart A., Zhang Y., Varki A.
      J. Biol. Chem. 274:25623-25631(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: B-cell.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Strain: C57BL/6J.
      Tissue: Blastocyst, Cerebellum, Diencephalon, Embryonic head, Skin and Urinary bladder.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

    Entry informationi

    Entry nameiSIAE_MOUSE
    AccessioniPrimary (citable) accession number: P70665
    Secondary accession number(s): Q3TNZ5
    , Q544V7, Q61044, Q8C902, Q8CBM6, Q8CC41, Q8CEB7, Q922L0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: May 30, 2006
    Last modified: October 1, 2014
    This is version 106 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3