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Protein

SPARC-like protein 1

Gene

Sparcl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi621 – 632PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SPARC-like protein 1
Alternative name(s):
Extracellular matrix protein 2
Matrix glycoprotein Sc1
Gene namesi
Name:Sparcl1
Synonyms:Ecm2, Sc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:108110. Sparcl1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000002031317 – 650SPARC-like protein 1Add BLAST634

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70PhosphoserineBy similarity1
Modified residuei78PhosphoserineBy similarity1
Modified residuei86PhosphoserineBy similarity1
Glycosylationi148N-linked (GlcNAc...)Sequence analysis1
Modified residuei155PhosphoserineBy similarity1
Modified residuei163PhosphoserineBy similarity1
Glycosylationi168N-linked (GlcNAc...)Sequence analysis1
Modified residuei272PhosphoserineBy similarity1
Modified residuei353PhosphoserineCombined sources1
Modified residuei406PhosphoserineCombined sources1
Disulfide bondi419 ↔ 430PROSITE-ProRule annotation
Disulfide bondi424 ↔ 440PROSITE-ProRule annotation
Disulfide bondi442 ↔ 476PROSITE-ProRule annotation
Disulfide bondi448 ↔ 469PROSITE-ProRule annotation
Disulfide bondi458 ↔ 495PROSITE-ProRule annotation
Glycosylationi462N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi501 ↔ 612PROSITE-ProRule annotation
Disulfide bondi620 ↔ 636PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP70663.
PaxDbiP70663.
PeptideAtlasiP70663.
PRIDEiP70663.

PTM databases

iPTMnetiP70663.
PhosphoSitePlusiP70663.

Expressioni

Tissue specificityi

Highest expression in brain. Moderate levels in heart, adrenal gland, epididymis and lung. Low levels in kidney, eye, liver, spleen, submandibular gland and testis.

Gene expression databases

BgeeiENSMUSG00000029309.
CleanExiMM_SPARCL1.
ExpressionAtlasiP70663. baseline and differential.
GenevisibleiP70663. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199367. 1 interactor.
IntActiP70663. 2 interactors.
MINTiMINT-1177391.
STRINGi10090.ENSMUSP00000031249.

Structurei

3D structure databases

ProteinModelPortaliP70663.
SMRiP70663.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini418 – 440Follistatin-likeAdd BLAST23
Domaini436 – 497Kazal-likePROSITE-ProRule annotationAdd BLAST62
Domaini608 – 643EF-handPROSITE-ProRule annotationAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi192 – 277Glu-richAdd BLAST86
Compositional biasi192 – 209Poly-GluAdd BLAST18

Sequence similaritiesi

Belongs to the SPARC family.Curated
Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 follistatin-like domain.Curated
Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4004. Eukaryota.
ENOG41101WW. LUCA.
GeneTreeiENSGT00510000046787.
HOGENOMiHOG000063702.
HOVERGENiHBG079212.
InParanoidiP70663.
OMAiDYFGACK.
OrthoDBiEOG091G0CPG.
TreeFamiTF319356.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR003645. Fol_N.
IPR015369. Follistatin/Osteonectin_EGF.
IPR002350. Kazal_dom.
IPR001999. Osteonectin_CS.
IPR016359. SPARC-like_p1.
IPR019577. SPARC/Testican_Ca-bd-dom.
[Graphical view]
PfamiPF09289. FOLN. 1 hit.
PF00050. Kazal_1. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF002574. SPARC-like_p1. 1 hit.
SMARTiSM00274. FOLN. 1 hit.
SM00280. KAZAL. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS00612. OSTEONECTIN_1. 1 hit.
PS00613. OSTEONECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70663-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVLLLLCA LGTAVAIPTS TRFLSDHSNP TTATLVTPED ATVPIAGVEA
60 70 80 90 100
TADIENHPSD KAEKPSALNS EEETHEQSTE QDKTYSFEVD LKDEEDGDGD
110 120 130 140 150
LSVDPTEGTL TLDLQEGTSE PQQKSLPENG DFPATVSTSY VDPNQRANIT
160 170 180 190 200
KGKESQEQPV SDSHQQPNES SKQTQDLKAE ESQTQDPDIP NEEEEEEEDE
210 220 230 240 250
EEEEEEEPED IGAPSDNQEE GKEPLEEQPT SKWEGNREQS DDTLEESSQP
260 270 280 290 300
TQISKTEKHQ SEQGNQGQES DSEAEGEDKA AGSKEHIPHT EQQDQEGKAG
310 320 330 340 350
LEAIGNQKDT DEKAVSTEPT DAAVVPRSHG GAGDNGGGDD SKHGAGDDYF
360 370 380 390 400
IPSQEFLEAE RMHSLSYYLK YGGGEETTTG ESENRREAAD NQEAKKAESS
410 420 430 440 450
PNAEPSDEGN SREHSAGSCT NFQCKRGHIC KTDPQGKPHC VCQDPETCPP
460 470 480 490 500
AKILDQACGT DNQTYASSCH LFATKCRLEG TKKGHQLQLD YFGACKSIPA
510 520 530 540 550
CTDFEVAQFP LRMRDWLKNI LMQLYEPNPK HGGYLNEKQR SKVKKIYLDE
560 570 580 590 600
KRLLAGDHPI ELLLRDFKKN YHMYVYPVHW QFNELDQHPA DRILTHSELA
610 620 630 640 650
PLRASLVPME HCITRFFEEC DPNKDKHITL KEWGHCFGIK EEDIDENLLF
Length:650
Mass (Da):72,287
Last modified:July 27, 2011 - v3
Checksum:i8E2CA0145DE3E66E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25S → F in AAC53172 (PubMed:9199668).Curated1
Sequence conflicti59S → N in AAB06444 (PubMed:8938431).Curated1
Sequence conflicti59S → N in AAB08451 (PubMed:8938431).Curated1
Sequence conflicti59S → N in AAC53172 (PubMed:9199668).Curated1
Sequence conflicti59S → N in AAH03759 (PubMed:15489334).Curated1
Sequence conflicti199D → E in AAB06444 (PubMed:8938431).Curated1
Sequence conflicti199D → E in AAB08451 (PubMed:8938431).Curated1
Sequence conflicti281A → S in AAB06444 (PubMed:8938431).Curated1
Sequence conflicti281A → S in AAB08451 (PubMed:8938431).Curated1
Sequence conflicti385R → Q in AAB06444 (PubMed:8938431).Curated1
Sequence conflicti385R → Q in AAB08451 (PubMed:8938431).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66166
, U66158, U66159, U66160, U66161, U66162, U66163, U66164, U66165 Genomic DNA. Translation: AAB06444.1.
U77330 mRNA. Translation: AAC53172.1.
U64827 mRNA. Translation: AAB08451.1.
AL714024 Genomic DNA. No translation available.
BC003759 mRNA. Translation: AAH03759.1.
CCDSiCCDS19483.1.
RefSeqiNP_034227.3. NM_010097.4.
XP_006534831.1. XM_006534768.1.
XP_017176145.1. XM_017320656.1.
UniGeneiMm.29027.

Genome annotation databases

EnsembliENSMUST00000031249; ENSMUSP00000031249; ENSMUSG00000029309.
GeneIDi13602.
KEGGimmu:13602.
UCSCiuc008ykb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66166
, U66158, U66159, U66160, U66161, U66162, U66163, U66164, U66165 Genomic DNA. Translation: AAB06444.1.
U77330 mRNA. Translation: AAC53172.1.
U64827 mRNA. Translation: AAB08451.1.
AL714024 Genomic DNA. No translation available.
BC003759 mRNA. Translation: AAH03759.1.
CCDSiCCDS19483.1.
RefSeqiNP_034227.3. NM_010097.4.
XP_006534831.1. XM_006534768.1.
XP_017176145.1. XM_017320656.1.
UniGeneiMm.29027.

3D structure databases

ProteinModelPortaliP70663.
SMRiP70663.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199367. 1 interactor.
IntActiP70663. 2 interactors.
MINTiMINT-1177391.
STRINGi10090.ENSMUSP00000031249.

PTM databases

iPTMnetiP70663.
PhosphoSitePlusiP70663.

Proteomic databases

MaxQBiP70663.
PaxDbiP70663.
PeptideAtlasiP70663.
PRIDEiP70663.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031249; ENSMUSP00000031249; ENSMUSG00000029309.
GeneIDi13602.
KEGGimmu:13602.
UCSCiuc008ykb.2. mouse.

Organism-specific databases

CTDi8404.
MGIiMGI:108110. Sparcl1.

Phylogenomic databases

eggNOGiKOG4004. Eukaryota.
ENOG41101WW. LUCA.
GeneTreeiENSGT00510000046787.
HOGENOMiHOG000063702.
HOVERGENiHBG079212.
InParanoidiP70663.
OMAiDYFGACK.
OrthoDBiEOG091G0CPG.
TreeFamiTF319356.

Miscellaneous databases

ChiTaRSiSparcl1. mouse.
PROiP70663.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029309.
CleanExiMM_SPARCL1.
ExpressionAtlasiP70663. baseline and differential.
GenevisibleiP70663. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR003645. Fol_N.
IPR015369. Follistatin/Osteonectin_EGF.
IPR002350. Kazal_dom.
IPR001999. Osteonectin_CS.
IPR016359. SPARC-like_p1.
IPR019577. SPARC/Testican_Ca-bd-dom.
[Graphical view]
PfamiPF09289. FOLN. 1 hit.
PF00050. Kazal_1. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF002574. SPARC-like_p1. 1 hit.
SMARTiSM00274. FOLN. 1 hit.
SM00280. KAZAL. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS00612. OSTEONECTIN_1. 1 hit.
PS00613. OSTEONECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPRL1_MOUSE
AccessioniPrimary (citable) accession number: P70663
Secondary accession number(s): E9QPH2, P97810, Q99L82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.