ID   NGN3_MOUSE              Reviewed;         214 AA.
AC   P70661;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   24-JAN-2024, entry version 172.
DE   RecName: Full=Neurogenin-3;
DE            Short=NGN-3;
DE   AltName: Full=Helix-loop-helix protein mATH-4B;
DE            Short=mATH4B;
DE   AltName: Full=Protein atonal homolog 5;
GN   Name=Neurog3; Synonyms=Ath4b, Atoh5, Ngn3;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/Sv;
RX   PubMed=9108377; DOI=10.1242/dev.124.8.1611;
RA   Cau E., Gradwohl G., Fode C., Guillemot F.;
RT   "Mash1 activates a cascade of bHLH regulators in olfactory neuron
RT   progenitors.";
RL   Development 124:1611-1621(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9000438; DOI=10.1006/mcne.1996.0060;
RA   Sommer L., Ma Q., Anderson D.J.;
RT   "Neurogenins, a novel family of atonal-related bHLH transcription factors,
RT   are putative mammalian neuronal determination genes that reveal progenitor
RT   cell heterogeneity in the developing CNS and PNS.";
RL   Mol. Cell. Neurosci. 8:221-241(1996).
RN   [3]
RP   FUNCTION.
RX   PubMed=10677506; DOI=10.1073/pnas.97.4.1607;
RA   Gradwohl G., Dierich A., LeMeur M., Guillemot F.;
RT   "Neurogenin3 is required for the development of the four endocrine cell
RT   lineages of the pancreas.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:1607-1611(2000).
RN   [4]
RP   INTERACTION WITH ATOH8.
RX   PubMed=18560595; DOI=10.1371/journal.pone.0002430;
RA   Lynn F.C., Sanchez L., Gomis R., German M.S., Gasa R.;
RT   "Identification of the bHLH factor Math6 as a novel component of the
RT   embryonic pancreas transcriptional network.";
RL   PLoS ONE 3:E2430-E2430(2008).
RN   [5]
RP   FUNCTION.
RX   PubMed=19759004; DOI=10.1074/jbc.m109.048694;
RA   Anderson K.R., Torres C.A., Solomon K., Becker T.C., Newgard C.B.,
RA   Wright C.V., Hagman J., Sussel L.;
RT   "Cooperative transcriptional regulation of the essential pancreatic islet
RT   gene NeuroD1 (beta2) by Nkx2.2 and neurogenin 3.";
RL   J. Biol. Chem. 284:31236-31248(2009).
CC   -!- FUNCTION: Acts as a transcriptional regulator. Together with NKX2-2,
CC       initiates transcriptional activation of NEUROD1. Involved in
CC       neurogenesis. Also required for the specification of a common precursor
CC       of the 4 pancreatic endocrine cell types. {ECO:0000269|PubMed:10677506,
CC       ECO:0000269|PubMed:19759004}.
CC   -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC       protein. Interacts with ATOH8. {ECO:0000269|PubMed:18560595}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
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DR   EMBL; Y09167; CAA70366.1; -; Genomic_DNA.
DR   EMBL; U76208; AAC53029.1; -; Genomic_DNA.
DR   CCDS; CCDS23887.1; -.
DR   RefSeq; NP_033849.3; NM_009719.6.
DR   AlphaFoldDB; P70661; -.
DR   SMR; P70661; -.
DR   STRING; 10090.ENSMUSP00000054054; -.
DR   iPTMnet; P70661; -.
DR   PhosphoSitePlus; P70661; -.
DR   PaxDb; 10090-ENSMUSP00000054054; -.
DR   Antibodypedia; 28907; 600 antibodies from 33 providers.
DR   DNASU; 11925; -.
DR   Ensembl; ENSMUST00000050103.2; ENSMUSP00000054054.2; ENSMUSG00000044312.5.
DR   GeneID; 11925; -.
DR   KEGG; mmu:11925; -.
DR   UCSC; uc007fgu.1; mouse.
DR   AGR; MGI:893591; -.
DR   CTD; 50674; -.
DR   MGI; MGI:893591; Neurog3.
DR   VEuPathDB; HostDB:ENSMUSG00000044312; -.
DR   eggNOG; KOG3898; Eukaryota.
DR   GeneTree; ENSGT00940000163077; -.
DR   HOGENOM; CLU_097959_1_0_1; -.
DR   InParanoid; P70661; -.
DR   OMA; ADHSFYG; -.
DR   OrthoDB; 2915590at2759; -.
DR   PhylomeDB; P70661; -.
DR   TreeFam; TF315153; -.
DR   BioGRID-ORCS; 11925; 4 hits in 77 CRISPR screens.
DR   ChiTaRS; Neurog3; mouse.
DR   PRO; PR:P70661; -.
DR   Proteomes; UP000000589; Chromosome 10.
DR   RNAct; P70661; Protein.
DR   Bgee; ENSMUSG00000044312; Expressed in foregut-midgut junction and 46 other cell types or tissues.
DR   ExpressionAtlas; P70661; baseline and differential.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0031490; F:chromatin DNA binding; IDA:UniProtKB.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR   GO; GO:0003690; F:double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0070888; F:E-box binding; IBA:GO_Central.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:UniProtKB.
DR   GO; GO:0061564; P:axon development; IBA:GO_Central.
DR   GO; GO:0030855; P:epithelial cell differentiation; IMP:MGI.
DR   GO; GO:0030900; P:forebrain development; IBA:GO_Central.
DR   GO; GO:0030902; P:hindbrain development; IEA:Ensembl.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR   GO; GO:0045893; P:positive regulation of DNA-templated transcription; IDA:GO_Central.
DR   GO; GO:0045666; P:positive regulation of neuron differentiation; ISO:MGI.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR   GO; GO:0048814; P:regulation of dendrite morphogenesis; IGI:MGI.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; ISO:MGI.
DR   GO; GO:0007423; P:sensory organ development; IBA:GO_Central.
DR   GO; GO:0021510; P:spinal cord development; IEA:Ensembl.
DR   GO; GO:0060290; P:transdifferentiation; IDA:UniProtKB.
DR   CDD; cd19718; bHLH_TS_NGN3_ATOH5; 1.
DR   Gene3D; 4.10.280.10; Helix-loop-helix DNA-binding domain; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR032656; Ngn3_bHLH.
DR   PANTHER; PTHR19290; BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED; 1.
DR   PANTHER; PTHR19290:SF94; NEUROGENIN-3; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; HLH, helix-loop-helix DNA-binding domain; 1.
DR   PROSITE; PS50888; BHLH; 1.
DR   Genevisible; P70661; MM.
PE   1: Evidence at protein level;
KW   Activator; Developmental protein; Differentiation; DNA-binding;
KW   Neurogenesis; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..214
FT                   /note="Neurogenin-3"
FT                   /id="PRO_0000127403"
FT   DOMAIN          83..135
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          1..98
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        11..41
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   214 AA;  23267 MW;  1AB31183291FB98B CRC64;
     MAPHPLDALT IQVSPETQQP FPGASDHEVL SSNSTPPSPT LIPRDCSEAE VGDCRGTSRK
     LRARRGGRNR PKSELALSKQ RRSRRKKAND RERNRMHNLN SALDALRGVL PTFPDDAKLT
     KIETLRFAHN YIWALTQTLR IADHSFYGPE PPVPCGELGS PGGGSNGDWG SIYSPVSQAG
     NLSPTASLEE FPGLQVPSSP SYLLPGALVF SDFL
//