P70661 (NGN3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neurogenin-3
Short name=NGN-3

Alternative name(s):
Helix-loop-helix protein mATH-4B
Short name=mATH4B
Protein atonal homolog 5

Gene names

Name:	Neurog3
Synonyms:	Ath4b, Atoh5, Ngn3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a transcriptional regulator. Together with NKX2-2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. Ref.3 Ref.4
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein.
Subcellular location	Nucleus Potential.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocrine pancreas development Traceable author statement. Source: Reactome epithelial cell differentiation Inferred from mutant phenotype PubMed 12080087. Source: MGI forebrain development Inferred from electronic annotation. Source: Compara hindbrain development Inferred from electronic annotation. Source: Compara positive regulation of neuron differentiation Inferred from electronic annotation. Source: Compara positive regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.4. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 16511571. Source: MGI spinal cord development Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Traceable author statement. Source: Reactome nucleus Inferred from direct assay PubMed 12921743. Source: MGI
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from electronic annotation. Source: Compara double-stranded DNA binding Inferred from electronic annotation. Source: Compara sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 16511571. Source: MGI transcription coactivator activity Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Neurogenin-3		PRO_0000127403
Regions
	Domain	83 – 135	53	bHLH

Sequences

	Sequence		Length	Mass (Da)
	P70661 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 1AB31183291FB98B	FASTA	214	23,267
	10 20 30 40 50 60 MAPHPLDALT IQVSPETQQP FPGASDHEVL SSNSTPPSPT LIPRDCSEAE VGDCRGTSRK 70 80 90 100 110 120 LRARRGGRNR PKSELALSKQ RRSRRKKAND RERNRMHNLN SALDALRGVL PTFPDDAKLT 130 140 150 160 170 180 KIETLRFAHN YIWALTQTLR IADHSFYGPE PPVPCGELGS PGGGSNGDWG SIYSPVSQAG 190 200 210 NLSPTASLEE FPGLQVPSSP SYLLPGALVF SDFL

References

[1]	"Mash1 activates a cascade of bHLH regulators in olfactory neuron progenitors." Cau E., Gradwohl G., Fode C., Guillemot F. Development 124:1611-1621(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[2]	"Neurogenins, a novel family of atonal-related bHLH transcription factors, are putative mammalian neuronal determination genes that reveal progenitor cell heterogeneity in the developing CNS and PNS." Sommer L., Ma Q., Anderson D.J. Mol. Cell. Neurosci. 8:221-241(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas." Gradwohl G., Dierich A., LeMeur M., Guillemot F. Proc. Natl. Acad. Sci. U.S.A. 97:1607-1611(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Cooperative transcriptional regulation of the essential pancreatic islet gene NeuroD1 (beta2) by Nkx2.2 and neurogenin 3." Anderson K.R., Torres C.A., Solomon K., Becker T.C., Newgard C.B., Wright C.V., Hagman J., Sussel L. J. Biol. Chem. 284:31236-31248(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09167 Genomic DNA. Translation: CAA70366.1. U76208 Genomic DNA. Translation: AAC53029.1.
IPI	IPI00111788.
RefSeq	NP_033849.3. NM_009719.6.
UniGene	Mm.57236.
3D structure databases
ProteinModelPortal	P70661.
SMR	P70661. Positions 83-140.
ModBase	Search...
PTM databases
PhosphoSite	P70661.
Proteomic databases
PRIDE	P70661.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000050103; ENSMUSP00000054054; ENSMUSG00000044312.
GeneID	11925.
KEGG	mmu:11925.
Organism-specific databases
CTD	50674.
MGI	MGI:893591. Neurog3.
Phylogenomic databases
eggNOG	NOG327835.
HOGENOM	HOG000063655.
HOVERGEN	HBG031743.
InParanoid	P70661.
KO	K08028.
OMA	KKANDRE.
OrthoDB	EOG4JM7R1.
Enzyme and pathway databases
Reactome	REACT_127416. Developmental Biology. REACT_13641. Regulation of Beta-Cell Development.
Gene expression databases
ArrayExpress	P70661.
Bgee	P70661.
CleanEx	MM_NEUROG3.
Genevestigator	P70661.
GermOnline	ENSMUSG00000044312. Mus musculus.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR011598. bHLH_dom. [Graphical view]
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	280005.
SOURCE	Search...

Entry information

Entry name

NGN3_MOUSE

Accession

Primary (citable) accession number: P70661

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program