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P70661 (NGN3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neurogenin-3

Short name=NGN-3
Alternative name(s):
Helix-loop-helix protein mATH-4B
Short name=mATH4B
Protein atonal homolog 5
Gene names
Name:Neurog3
Synonyms:Ath4b, Atoh5, Ngn3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Acts as a transcriptional regulator. Together with NKX2-2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. Ref.3 Ref.4

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein.

Subcellular location

Nucleus Potential.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Biological processDifferentiation
Neurogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processendocrine pancreas development

Traceable author statement. Source: Reactome

epithelial cell differentiation

Inferred from mutant phenotype PubMed 12080087. Source: MGI

forebrain development

Inferred from electronic annotation. Source: Ensembl

hindbrain development

Inferred from electronic annotation. Source: Ensembl

multicellular organismal development

Inferred from mutant phenotype PubMed 12080087. Source: MGI

positive regulation of neuron differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.4. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16511571. Source: MGI

regulation of dendrite morphogenesis

Inferred from genetic interaction PubMed 21818269. Source: MGI

spinal cord development

Inferred from electronic annotation. Source: Ensembl

transdifferentiation

Inferred from direct assay PubMed 16511571. Source: UniProtKB

   Cellular_componentcytosol

Traceable author statement. Source: Reactome

intracellular

Inferred from direct assay PubMed 10868931PubMed 15793245. Source: MGI

nucleus

Inferred from direct assay PubMed 12921743. Source: MGI

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from electronic annotation. Source: Ensembl

chromatin DNA binding

Inferred from direct assay PubMed 16511571. Source: UniProtKB

core promoter binding

Inferred from direct assay PubMed 16511571. Source: UniProtKB

double-stranded DNA binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 16511571. Source: MGI

transcription coactivator activity

Inferred from direct assay Ref.4. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 214214Neurogenin-3
PRO_0000127403

Regions

Domain83 – 13553bHLH

Sequences

Sequence LengthMass (Da)Tools
P70661 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 1AB31183291FB98B

FASTA21423,267
        10         20         30         40         50         60 
MAPHPLDALT IQVSPETQQP FPGASDHEVL SSNSTPPSPT LIPRDCSEAE VGDCRGTSRK 

        70         80         90        100        110        120 
LRARRGGRNR PKSELALSKQ RRSRRKKAND RERNRMHNLN SALDALRGVL PTFPDDAKLT 

       130        140        150        160        170        180 
KIETLRFAHN YIWALTQTLR IADHSFYGPE PPVPCGELGS PGGGSNGDWG SIYSPVSQAG 

       190        200        210 
NLSPTASLEE FPGLQVPSSP SYLLPGALVF SDFL 

« Hide

References

[1]"Mash1 activates a cascade of bHLH regulators in olfactory neuron progenitors."
Cau E., Gradwohl G., Fode C., Guillemot F.
Development 124:1611-1621(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[2]"Neurogenins, a novel family of atonal-related bHLH transcription factors, are putative mammalian neuronal determination genes that reveal progenitor cell heterogeneity in the developing CNS and PNS."
Sommer L., Ma Q., Anderson D.J.
Mol. Cell. Neurosci. 8:221-241(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas."
Gradwohl G., Dierich A., LeMeur M., Guillemot F.
Proc. Natl. Acad. Sci. U.S.A. 97:1607-1611(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Cooperative transcriptional regulation of the essential pancreatic islet gene NeuroD1 (beta2) by Nkx2.2 and neurogenin 3."
Anderson K.R., Torres C.A., Solomon K., Becker T.C., Newgard C.B., Wright C.V., Hagman J., Sussel L.
J. Biol. Chem. 284:31236-31248(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09167 Genomic DNA. Translation: CAA70366.1.
U76208 Genomic DNA. Translation: AAC53029.1.
CCDSCCDS23887.1.
RefSeqNP_033849.3. NM_009719.6.
UniGeneMm.57236.

3D structure databases

ProteinModelPortalP70661.
SMRP70661. Positions 83-140.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP70661.

Proteomic databases

PRIDEP70661.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000050103; ENSMUSP00000054054; ENSMUSG00000044312.
GeneID11925.
KEGGmmu:11925.
UCSCuc007fgu.1. mouse.

Organism-specific databases

CTD50674.
MGIMGI:893591. Neurog3.

Phylogenomic databases

eggNOGNOG327835.
HOGENOMHOG000063655.
HOVERGENHBG031743.
InParanoidP70661.
KOK08028.
OMARKKANDR.
OrthoDBEOG7TTQB1.
PhylomeDBP70661.
TreeFamTF315153.

Enzyme and pathway databases

ReactomeREACT_13641. Regulation of Beta-Cell Development.
REACT_188576. Developmental Biology.

Gene expression databases

BgeeP70661.
CleanExMM_NEUROG3.
GenevestigatorP70661.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
[Graphical view]
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio280005.
PROP70661.
SOURCESearch...

Entry information

Entry nameNGN3_MOUSE
AccessionPrimary (citable) accession number: P70661
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot