ID   NGN1_MOUSE              Reviewed;         244 AA.
AC   P70660;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   24-JAN-2024, entry version 157.
DE   RecName: Full=Neurogenin-1;
DE            Short=NGN-1;
DE   AltName: Full=Helix-loop-helix protein mATH-4C;
DE            Short=mATH4C;
DE   AltName: Full=Neurogenic basic-helix-loop-helix protein;
DE   AltName: Full=Neurogenic differentiation factor 3;
DE            Short=NeuroD3;
GN   Name=Neurog1; Synonyms=Ath4c, Neurod3, Ngn, Ngn1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/Sv;
RX   PubMed=8816493; DOI=10.1128/mcb.16.10.5792;
RA   McCormick M.B., Tamimi R.M., Snider L., Asakura A., Bergstrom D.,
RA   Tapscott S.J.;
RT   "NeuroD2 and neuroD3: distinct expression patterns and transcriptional
RT   activation potentials within the neuroD gene family.";
RL   Mol. Cell. Biol. 16:5792-5800(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/Sv;
RX   PubMed=8858147; DOI=10.1016/s0092-8674(00)81321-5;
RA   Ma Q., Kintner C., Anderson D.J.;
RT   "Identification of neurogenin, a vertebrate neuronal determination gene.";
RL   Cell 87:43-52(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/Sv;
RX   PubMed=9108377; DOI=10.1242/dev.124.8.1611;
RA   Cau E., Gradwohl G., Fode C., Guillemot F.;
RT   "Mash1 activates a cascade of bHLH regulators in olfactory neuron
RT   progenitors.";
RL   Development 124:1611-1621(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Bone;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   FUNCTION, AND DNA-BINDING.
RX   PubMed=10648228; DOI=10.1242/dev.127.4.693;
RA   Farah M.H., Olson J.M., Sucic H.B., Hume R.I., Tapscott S.J., Turner D.L.;
RT   "Generation of neurons by transient expression of neural bHLH proteins in
RT   mammalian cells.";
RL   Development 127:693-702(2000).
RN   [6]
RP   FUNCTION, AND DNA-BINDING.
RX   PubMed=14697366; DOI=10.1016/j.ydbio.2003.08.027;
RA   Lin C.H., Stoeck J., Ravanpay A.C., Guillemot F., Tapscott S.J.,
RA   Olson J.M.;
RT   "Regulation of neuroD2 expression in mouse brain.";
RL   Dev. Biol. 265:234-245(2004).
RN   [7]
RP   FUNCTION, AND ASSOCIATION WITH CHROMATIN.
RX   PubMed=18007592; DOI=10.1038/sj.emboj.7601923;
RA   Seo S., Lim J.W., Yellajoshyula D., Chang L.W., Kroll K.L.;
RT   "Neurogenin and NeuroD direct transcriptional targets and their regulatory
RT   enhancers.";
RL   EMBO J. 26:5093-5108(2007).
CC   -!- FUNCTION: Acts as a transcriptional regulator. Involved in the
CC       initiation of neuronal differentiation. Activates transcription by
CC       binding to the E box (5'-CANNTG-3'). Associates with chromatin to
CC       enhancer regulatory elements in genes encoding key transcriptional
CC       regulators of neurogenesis. {ECO:0000269|PubMed:10648228,
CC       ECO:0000269|PubMed:14697366, ECO:0000269|PubMed:18007592}.
CC   -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC       protein.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expression restricted to the embryonic nervous
CC       system.
CC   -!- DEVELOPMENTAL STAGE: Highest expression in the embryo between days 10
CC       and 12. Declines to undetectable levels by embryonic day 16.
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DR   EMBL; U63841; AAB37576.1; -; Genomic_DNA.
DR   EMBL; U67776; AAC52856.1; -; Genomic_DNA.
DR   EMBL; Y09166; CAA70365.1; -; Genomic_DNA.
DR   EMBL; BC062148; AAH62148.1; -; mRNA.
DR   CCDS; CCDS26559.1; -.
DR   RefSeq; NP_035026.1; NM_010896.2.
DR   AlphaFoldDB; P70660; -.
DR   SMR; P70660; -.
DR   BioGRID; 201735; 4.
DR   IntAct; P70660; 1.
DR   STRING; 10090.ENSMUSP00000050484; -.
DR   iPTMnet; P70660; -.
DR   PhosphoSitePlus; P70660; -.
DR   PaxDb; 10090-ENSMUSP00000050484; -.
DR   Antibodypedia; 26408; 652 antibodies from 34 providers.
DR   DNASU; 18014; -.
DR   Ensembl; ENSMUST00000058475.6; ENSMUSP00000050484.5; ENSMUSG00000048904.6.
DR   GeneID; 18014; -.
DR   KEGG; mmu:18014; -.
DR   UCSC; uc007qsl.2; mouse.
DR   AGR; MGI:107754; -.
DR   CTD; 4762; -.
DR   MGI; MGI:107754; Neurog1.
DR   VEuPathDB; HostDB:ENSMUSG00000048904; -.
DR   eggNOG; KOG3898; Eukaryota.
DR   GeneTree; ENSGT00940000162170; -.
DR   HOGENOM; CLU_097959_0_0_1; -.
DR   InParanoid; P70660; -.
DR   OMA; SEDYCYG; -.
DR   OrthoDB; 2915590at2759; -.
DR   PhylomeDB; P70660; -.
DR   TreeFam; TF315153; -.
DR   BioGRID-ORCS; 18014; 3 hits in 78 CRISPR screens.
DR   PRO; PR:P70660; -.
DR   Proteomes; UP000000589; Chromosome 13.
DR   RNAct; P70660; Protein.
DR   Bgee; ENSMUSG00000048904; Expressed in cerebral cortex ventricular layer and 92 other cell types or tissues.
DR   GO; GO:0043025; C:neuronal cell body; IDA:MGI.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0043204; C:perikaryon; IDA:MGI.
DR   GO; GO:0003682; F:chromatin binding; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; ISO:MGI.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0070888; F:E-box binding; IDA:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0031223; P:auditory behavior; ISO:MGI.
DR   GO; GO:0061564; P:axon development; IBA:GO_Central.
DR   GO; GO:0045165; P:cell fate commitment; IGI:MGI.
DR   GO; GO:0090102; P:cochlea development; ISO:MGI.
DR   GO; GO:0090103; P:cochlea morphogenesis; ISO:MGI.
DR   GO; GO:0097094; P:craniofacial suture morphogenesis; ISO:MGI.
DR   GO; GO:0010458; P:exit from mitosis; IMP:MGI.
DR   GO; GO:0030900; P:forebrain development; IBA:GO_Central.
DR   GO; GO:0048806; P:genitalia development; ISO:MGI.
DR   GO; GO:0035112; P:genitalia morphogenesis; ISO:MGI.
DR   GO; GO:1905748; P:hard palate morphogenesis; ISO:MGI.
DR   GO; GO:0048839; P:inner ear development; ISO:MGI.
DR   GO; GO:0042472; P:inner ear morphogenesis; IMP:MGI.
DR   GO; GO:0098583; P:learned vocalization behavior; ISO:MGI.
DR   GO; GO:0071626; P:mastication; ISO:MGI.
DR   GO; GO:1901078; P:negative regulation of relaxation of muscle; ISO:MGI.
DR   GO; GO:1905747; P:negative regulation of saliva secretion; ISO:MGI.
DR   GO; GO:0022008; P:neurogenesis; IDA:MGI.
DR   GO; GO:0050885; P:neuromuscular process controlling balance; ISO:MGI.
DR   GO; GO:0030182; P:neuron differentiation; IDA:MGI.
DR   GO; GO:0030432; P:peristalsis; ISO:MGI.
DR   GO; GO:0051091; P:positive regulation of DNA-binding transcription factor activity; IDA:UniProtKB.
DR   GO; GO:0031536; P:positive regulation of exit from mitosis; IMP:MGI.
DR   GO; GO:0045666; P:positive regulation of neuron differentiation; IDA:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR   GO; GO:0048634; P:regulation of muscle organ development; ISO:MGI.
DR   GO; GO:0045664; P:regulation of neuron differentiation; IGI:MGI.
DR   GO; GO:0007423; P:sensory organ development; IBA:GO_Central.
DR   GO; GO:0007356; P:thorax and anterior abdomen determination; ISO:MGI.
DR   GO; GO:0021559; P:trigeminal nerve development; ISO:MGI.
DR   GO; GO:0021650; P:vestibulocochlear nerve formation; ISO:MGI.
DR   CDD; cd19716; bHLH_TS_NGN1_NeuroD3; 1.
DR   Gene3D; 4.10.280.10; Helix-loop-helix DNA-binding domain; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   PANTHER; PTHR19290; BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED; 1.
DR   PANTHER; PTHR19290:SF130; NEUROGENIN-1; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; HLH, helix-loop-helix DNA-binding domain; 1.
DR   PROSITE; PS50888; BHLH; 1.
DR   Genevisible; P70660; MM.
PE   1: Evidence at protein level;
KW   Activator; Developmental protein; Differentiation; DNA-binding;
KW   Neurogenesis; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..244
FT                   /note="Neurogenin-1"
FT                   /id="PRO_0000127397"
FT   DOMAIN          93..145
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          1..27
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          39..82
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        10..27
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   244 AA;  26300 MW;  3C9C0E1A08AB074B CRC64;
     MPAPLETCIS DLDCSSSNSS SDLSSFLTDE EDCARLQPLA STSGLSVPAR RSAPALSGAS
     NVPGAQDEEQ ERRRRRGRAR VRSEALLHSL RRSRRVKAND RERNRMHNLN AALDALRSVL
     PSFPDDTKLT KIETLRFAYN YIWALAETLR LADQGLPGGS ARERLLPPQC VPCLPGPPSP
     ASDTESWGSG AAASPCATVA SPLSDPSSPS ASEDFTYGPG DPLFSFPGLP KDLLHTTPCF
     IPYH
//