P70660 (NGN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neurogenin-1
Short name=NGN-1

Alternative name(s):
Helix-loop-helix protein mATH-4C
Short name=mATH4C
Neurogenic basic-helix-loop-helix protein
Neurogenic differentiation factor 3
Short name=NeuroD3

Gene names

Name:	Neurog1
Synonyms:	Ath4c, Neurod3, Ngn, Ngn1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis. Ref.5 Ref.6 Ref.7
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein.
Subcellular location	Nucleus Probable.
Tissue specificity	Expression restricted to the embryonic nervous system.
Developmental stage	Highest expression in the embryo between days 10 and 12. Declines to undetectable levels by embryonic day 16.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell fate commitment Inferred from genetic interaction PubMed 15229646. Source: MGI inner ear morphogenesis Inferred from mutant phenotype PubMed 16145671. Source: MGI neuron differentiation Inferred from mutant phenotype PubMed 16145671. Source: MGI positive regulation of exit from mitosis Inferred from mutant phenotype PubMed 16145671. Source: MGI positive regulation of neuron differentiation Inferred from direct assay Ref.5Ref.7. Source: UniProtKB positive regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.6. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.7. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perikaryon Inferred from direct assay PubMed 15065125. Source: MGI
Molecular_function	E-box binding Inferred from direct assay Ref.6. Source: UniProtKB chromatin binding Inferred from direct assay Ref.7. Source: UniProtKB transcription factor binding transcription factor activity Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Neurogenin-1		PRO_0000127397
Regions
	Domain	93 – 145	53	bHLH

Sequences

	Sequence		Length	Mass (Da)
	P70660 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 3C9C0E1A08AB074B	FASTA	244	26,300
	10 20 30 40 50 60 MPAPLETCIS DLDCSSSNSS SDLSSFLTDE EDCARLQPLA STSGLSVPAR RSAPALSGAS 70 80 90 100 110 120 NVPGAQDEEQ ERRRRRGRAR VRSEALLHSL RRSRRVKAND RERNRMHNLN AALDALRSVL 130 140 150 160 170 180 PSFPDDTKLT KIETLRFAYN YIWALAETLR LADQGLPGGS ARERLLPPQC VPCLPGPPSP 190 200 210 220 230 240 ASDTESWGSG AAASPCATVA SPLSDPSSPS ASEDFTYGPG DPLFSFPGLP KDLLHTTPCF IPYH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NeuroD2 and neuroD3: distinct expression patterns and transcriptional activation potentials within the neuroD gene family." McCormick M.B., Tamimi R.M., Snider L., Asakura A., Bergstrom D., Tapscott S.J. Mol. Cell. Biol. 16:5792-5800(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[2]	"Identification of neurogenin, a vertebrate neuronal determination gene." Ma Q., Kintner C., Anderson D.J. Cell 87:43-52(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[3]	"Mash1 activates a cascade of bHLH regulators in olfactory neuron progenitors." Cau E., Gradwohl G., Fode C., Guillemot F. Development 124:1611-1621(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone.
[5]	"Generation of neurons by transient expression of neural bHLH proteins in mammalian cells." Farah M.H., Olson J.M., Sucic H.B., Hume R.I., Tapscott S.J., Turner D.L. Development 127:693-702(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DNA-BINDING.
[6]	"Regulation of neuroD2 expression in mouse brain." Lin C.H., Stoeck J., Ravanpay A.C., Guillemot F., Tapscott S.J., Olson J.M. Dev. Biol. 265:234-245(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DNA-BINDING.
[7]	"Neurogenin and NeuroD direct transcriptional targets and their regulatory enhancers." Seo S., Lim J.W., Yellajoshyula D., Chang L.W., Kroll K.L. EMBO J. 26:5093-5108(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ASSOCIATION WITH CHROMATIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U63841 Genomic DNA. Translation: AAB37576.1. U67776 Genomic DNA. Translation: AAC52856.1. Y09166 Genomic DNA. Translation: CAA70365.1. BC062148 mRNA. Translation: AAH62148.1.
IPI	IPI00111785.
RefSeq	NP_035026.1. NM_010896.2.
UniGene	Mm.266665.
3D structure databases
ProteinModelPortal	P70660.
SMR	P70660. Positions 93-150.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000050484.
PTM databases
PhosphoSite	P70660.
Proteomic databases
PRIDE	P70660.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000058475; ENSMUSP00000050484; ENSMUSG00000048904.
GeneID	18014.
KEGG	mmu:18014.
UCSC	uc007qsl.1. mouse.
Organism-specific databases
CTD	4762.
MGI	MGI:107754. Neurog1.
Phylogenomic databases
eggNOG	NOG275082.
GeneTree	ENSGT00680000099574.
HOGENOM	HOG000063655.
HOVERGEN	HBG101129.
InParanoid	P70660.
KO	K09081.
OMA	LLPPQCV.
OrthoDB	EOG4THVVQ.
Gene expression databases
Bgee	P70660.
CleanEx	MM_NEUROG1.
Genevestigator	P70660.
GermOnline	ENSMUSG00000048904. Mus musculus.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR011598. bHLH_dom. [Graphical view]
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	293045.
SOURCE	Search...

Entry information

Entry name

NGN1_MOUSE

Accession

Primary (citable) accession number: P70660

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program