Reviewed,
UniProtKB/Swiss-Prot P70658 (CXCR4_MOUSE)
Last modified
June 16, 2009.
Version 86.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: C-X-C chemokine receptor type 4 Short name=CXC-R4 Short name=CXCR-4 Alternative name(s): Stromal cell-derived factor 1 receptor Short name=SDF-1 receptor Fusin Leukocyte-derived seven transmembrane domain receptor Short name=LESTR Pre-B-cell-derived chemokine receptor Short name=PB-CKR CD_antigen=CD184 | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 359 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Receptor for the C-X-C chemokine CXCL12/SDF-1. Transduces a signal by increasing the intracellular calcium ions level. Involved in B-cell lymphopoiesis, bone-marrow myelopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar neuronal layer formation, preventing premature migration of proliferating granule cells from the external granule layer inwards. In the CNS, could mediate hippocampal-neuron survival. Ref.9 Ref.10 |
| Subunit structure | Monomer. Can form dimers By similarity. |
| Subcellular location | |
| Tissue specificity | Lymphocytes, macrophages, neutrophils, microglial cells and astrocytes. Found in spleen, thymus, bone marrow, lymph nodes and, at lower levels in brain, small intestine, stomach and kidney. CXCR4-A is predominant in all tissues tested. |
| Developmental stage | High expression during embryonic development does not seem to be associated with the differentiation of any particular cell type, but is widely utilized when there is a requirement for cell movement. Frequently associated with less differentiated cell types and down-regulated with subsequent differentiation. Detected in sites with haemopoietic potential: the yolk sac (7.5, 8.5 and 12.5 dpc) and fetal liver (12.5 dpc). During gastrulation, at 7.2 to 7.8 dpc, expressed in the mesoderm and the definitive endoderm. As gastrulation pattern fades (8.5 dpc), expression in the mesoderm is down-regulated, while it becomes predominant in neural ectoderm. Endodermal expression is retained in the foregut and later in a subset of foregut derivatives, including the stomach (10.5 dpc), the cystic ducts of the gall bladder and the lung epithelium (12.5 dpc). In neuronal tissue: at 10.5 and 12.5 dpc, expressed in the dorsal root ganglia, in the ventral mantle layer of the spinal cord (or basal plates), in the hindbrain. At 14.5 dpc, expression more tightly confined to the neural epithelium lining the ventricular space and to the external granular layer of the ventral rhombic lip (the developing cerebellum). Expressed in the outpocketing of the diencephalic floor at 10.5 dpc and in the developing thalamus and, to a lesser extent, the developing hypothalamus. At 14.5 dpc, restricted to the region where thalamus and hypothalamus meet. Detected in a discrete band of cells at the edge of the olfactory bulb. In the vascular system: expressed in the endothelium of numerous blood vessels, but not all, at 10.5, 11.5 and 12.5 dpc, such as vitelline/umbilical vessels, cardiac ventricular wall capillaries, facial vessels and, at 14.5 pdc, in the vasculature of the herniated gut. Expression seems to be associated with expanding vascular networks. In the heart development, expressed at 10.5 dpc in the precursor to the aortopulmonary (AP) septum. At 12.5 dpc, detected in the AP septum at the base of the outflow tract and in the atrioventricular valves. Detected in cranofacial ectoderm from 10.5 to 14.5 dpc. At 10.5 and 11.5 dpc, expressed in the Rathke pouch. Ref.11 |
| Post-translational modification | Sulfation is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization By similarity. O- and N-glycosylated. N-glycosylation can mask coreceptor function. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity By similarity. |
| Sequence similarities | Belongs to the G-protein coupled receptor 1 family. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform CXCR4-B (identifier: P70658-1) Also known as: LESTR-B; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform CXCR4-A (identifier: P70658-2) Also known as: LESTR-A; The sequence of this isoform differs from the canonical sequence as follows: 6-7: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 359 | 359 | C-X-C chemokine receptor type 4 | PRO_0000069355 | |||||||
Regions | |||||||||||
| Topological domain | 1 – 41 | 41 | Extracellular Potential | ||||||||
| Transmembrane | 42 – 65 | 24 | 1 Potential | ||||||||
| Topological domain | 66 – 81 | 16 | Cytoplasmic Potential | ||||||||
| Transmembrane | 82 – 101 | 20 | 2 Potential | ||||||||
| Topological domain | 102 – 112 | 11 | Extracellular Potential | ||||||||
| Transmembrane | 113 – 134 | 22 | 3 Potential | ||||||||
| Topological domain | 135 – 156 | 22 | Cytoplasmic Potential | ||||||||
| Transmembrane | 157 – 177 | 21 | 4 Potential | ||||||||
| Topological domain | 178 – 207 | 30 | Extracellular Potential | ||||||||
| Transmembrane | 208 – 227 | 20 | 5 Potential | ||||||||
| Topological domain | 228 – 247 | 20 | Cytoplasmic Potential | ||||||||
| Transmembrane | 248 – 268 | 21 | 6 Potential | ||||||||
| Topological domain | 269 – 292 | 24 | Extracellular Potential | ||||||||
| Transmembrane | 293 – 312 | 20 | 7 Potential | ||||||||
| Topological domain | 313 – 359 | 47 | Cytoplasmic Potential | ||||||||
Amino acid modifications | |||||||||||
| Modified residue | 23 | 1 | Sulfotyrosine By similarity | ||||||||
| Modified residue | 326 | 1 | Phosphoserine By similarity | ||||||||
| Modified residue | 355 | 1 | Phosphoserine By similarity | ||||||||
| Modified residue | 358 | 1 | Phosphoserine By similarity | ||||||||
| Glycosylation | 13 | 1 | N-linked (GlcNAc...) By similarity | ||||||||
| Glycosylation | 20 | 1 | O-linked (Xyl...) (chondroitin sulfate) By similarity | ||||||||
| Disulfide bond | 111 ↔ 193 | By similarity | |||||||||
Natural variations | |||||||||||
| Alternative sequence | 6 – 7 | 2 | Missing in isoform CXCR4-A. | VSP_001891 | |||||||
Experimental info | |||||||||||
| Sequence conflict | 216 | 1 | I → V Ref.4 | ||||||||
| Sequence conflict | 216 | 1 | I → V Ref.6 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning of the mouse fusin gene, homologue to a human HIV-1 co-factor." Heesen M., Berman M.A., Benson J.D., Gerard C., Dorf M.E. J. Immunol. 157:5455-5460(1996) [PubMed: 8955194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM CXCR4-B). Strain: 129/Sv and C57BL/6J. Tissue: Peritoneal exudate. |
| [2] | "Molecular cloning and characterization of a murine pre-B-cell growth-stimulating factor/stromal cell-derived factor 1 receptor, a murine homolog of the human immunodeficiency virus 1 entry coreceptor fusin." Nagasawa T., Nakajima T., Tachibana K., Iizasa H., Bleul C.C., Yoshie O., Matsushima K., Yoshida N., Springer T.A., Kishimoto T. Proc. Natl. Acad. Sci. U.S.A. 93:14726-14729(1996) [PubMed: 8962122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CXCR4-B). Tissue: Pre-B cell. |
| [3] | Schubel A., Burgstahler R., Lipp M. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CXCR4-B). Strain: 129/Sv. Tissue: Thymus. |
| [4] | "Two murine homologues of the human chemokine receptor CXCR4 mediating stromal cell-derived factor 1alpha activation of Gi2 are differentially expressed in vivo." Moepps B., Frodl R., Rodewald H.-R., Baggiolini M., Gierschik P. Eur. J. Immunol. 27:2102-2112(1997) [PubMed: 9295051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS CXCR4-A AND CXCR4-B). Strain: C57BL/6J X CBA/J. Tissue: Thymus. |
| [5] | "Alternate splicing of mouse fusin/CXC chemokine receptor-4: stromal cell-derived factor-1alpha is a ligand for both CXC chemokine receptor-4 isoforms." Heesen M., Berman M.A., Hoepken U.E., Gerard N.P., Dorf M.E. J. Immunol. 158:3561-3564(1997) [PubMed: 9103415] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS CXCR4-A AND CXCR4-B). |
| [6] | Suzuki G., Nakata Y., Uzawa A., Shirasawa T., Saito T., Mita K. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CXCR4-B). Strain: C57BL/6. Tissue: Thymus. |
| [7] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CXCR4-B). Strain: FVB/N. Tissue: Mammary gland. |
| [8] | "Genomic organization and expression of the CXCR4 gene in mouse and man: absence of a splice variant corresponding to mouse CXCR4-B in human tissues." Frodl R., Gierschik P., Moepps B. J. Recept. Signal Transduct. 18:321-344(1998) [PubMed: 9879064] [Abstract] Cited for: ALTERNATIVE SPLICING. |
| [9] | "The chemokine receptor CXCR4 is essential for vascularization of the gastrointestinal tract." Tachibana K., Hirota S., Iizasa H., Yoshida H., Kawabata K., Kataoka Y., Kitamura Y., Matsushima K., Yoshida N., Nishikawa S., Kishimoto T., Nagasawa T. Nature 393:591-594(1998) [PubMed: 9634237] [Abstract] Cited for: FUNCTION. |
| [10] | "Function of the chemokine receptor CXCR4 in haematopoiesis and in cerebellar development." Zou Y.-R., Kottmann A.H., Kuroda M., Taniuchi I., Littman D.R. Nature 393:595-599(1998) [PubMed: 9634238] [Abstract] Cited for: FUNCTION. |
| [11] | "Embryonic expression and function of the chemokine SDF-1 and its receptor, CXCR4." McGrath K.E., Koniski A.D., Maltby K.M., McGann J.K., Palis J. Dev. Biol. 213:442-456(1999) [PubMed: 10479460] [Abstract] Cited for: DEVELOPMENTAL STAGE. Strain: ICR. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U59760 mRNA. Translation: AAB07725.1. U65580 Genomic DNA. Translation: AAC52953.1. D87747 mRNA. Translation: BAA13451.1. Z80111 mRNA. Translation: CAB02201.1. Z80112 mRNA. Translation: CAB02202.1. X99581 Genomic DNA. Translation: CAA67893.1. X99582 mRNA. Translation: CAA67894.1. AB000803 mRNA. Translation: BAA19187.1. BC031665 mRNA. Translation: AAH31665.1. BC098322 mRNA. Translation: AAH98322.1. | |
| IPI | IPI00111763. IPI00849797. |
| RefSeq | NP_034041.2. |
| UniGene | Mm.1401 |
3D structure databases | |
| ModBase | Search... |
Protein family/group databases | |
| GPCRDB | Search... |
PTM databases | |
| PhosphoSite | P70658. |
Proteomic databases | |
| PRIDE | P70658. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000045382. Mus musculus. [Contig view] |
| GeneID | 12767. |
| KEGG | mmu:12767. |
Organism-specific databases | |
| MGI | MGI:109563. Cxcr4. |
Phylogenomic databases | |
| HOGENOM | P70658. |
| HOVERGEN | P70658. |
Gene expression databases | |
| ArrayExpress | P70658. |
| Bgee | P70658. |
| CleanEx | MM_CXCR4. |
| GermOnline | ENSMUSG00000045382. Mus musculus. |
Family and domain databases | |
| InterPro | IPR000276. 7TM_GPCR_Rhodpsn. IPR000355. Chmkine_rcpt. IPR001277. CXC_4_rcpt. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view] |
| Pfam | PF00001. 7tm_1. 1 hit. [Graphical view] |
| PRINTS | PR00657. CCCHEMOKINER. PR00645. CXCCHMKINER4. PR00237. GPCRRHODOPSN. |
| PROSITE | PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 460845. |
| SOURCE | Search... |
Entry information
| Entry name | CXCR4_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P70658 Secondary accession number(s): O09059 Q4KMW1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| 7-transmembrane G-linked receptors List of 7-transmembrane G-linked receptor entries |
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


