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Protein

Bleomycin hydrolase

Gene

Blmh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity (By similarity). Binds single-stranded DNA with higher affinity than double-stranded DNA. May play an important role in the metabolism of antibiotics.By similarity

Catalytic activityi

Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.

pH dependencei

Optimum pH is 7.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73PROSITE-ProRule annotation1
Active sitei372PROSITE-ProRule annotation1
Active sitei396PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.40 5301

Protein family/group databases

MEROPSiC01.084

Names & Taxonomyi

Protein namesi
Recommended name:
Bleomycin hydrolase (EC:3.4.22.40)
Short name:
BH
Short name:
BLM hydrolase
Short name:
BMH
Gene namesi
Name:Blmh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1304668 Blmh

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000505531 – 454Bleomycin hydrolaseAdd BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei391N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP70645
PeptideAtlasiP70645
PRIDEiP70645

PTM databases

iPTMnetiP70645
PhosphoSitePlusiP70645

Expressioni

Tissue specificityi

Expressed at relatively higher levels in the stomach, esophagus, spleen, thymus and testis, and at lower levels in the skin, lung and skeletal muscle.

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

IntActiP70645, 1 interactor
STRINGi10116.ENSRNOP00000005125

Structurei

3D structure databases

ProteinModelPortaliP70645
SMRiP70645
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4128 Eukaryota
COG3579 LUCA
HOVERGENiHBG002388
InParanoidiP70645
OMAiGGQWDMI

Family and domain databases

CDDicd00585 Peptidase_C1B, 1 hit
InterProiView protein in InterPro
IPR000169 Pept_cys_AS
IPR004134 Peptidase_C1B
PANTHERiPTHR10363 PTHR10363, 1 hit
PfamiView protein in Pfam
PF03051 Peptidase_C1_2, 1 hit
PIRSFiPIRSF005700 PepC, 1 hit
PROSITEiView protein in PROSITE
PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequencei

Sequence statusi: Complete.

P70645-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNAGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQGAQ
60 70 80 90 100
HVFQHVVPQE GKPVTNQKSS GRCWIFSCLN VMRLPFMKKF NIEEFEFSQS
110 120 130 140 150
YLFFWDKVER CYFFLNAFVD TAQKKEPEDG RLVQYLLMNP TNDGGQWDML
160 170 180 190 200
VNIVEKYGVV PKKCFPESHT TEATRRMNDI LNHKMREFCI RLRNLVHSGA
210 220 230 240 250
TKGEISSTQD AMMEEIFRVV CICLGNPPET FTWEYRDKDK NYHKVGPITP
260 270 280 290 300
LQFYKEHVKP LFNMEDKICF VNDPRPQHKY NKLYTVDYLS NMVGGRKTLY
310 320 330 340 350
NNQPIDFLKK MVAASIRDGE AVWFGCDVGK HFNGKLGLSD MNVYDHELVF
360 370 380 390 400
GVSLKNMNKA ERLAFGESLM THAMTFTAVS EKDDQEGAFV KWRVENSWGE
410 420 430 440 450
DHGHKGYLCM TDEWFSEYVY EVVVDKKHVP EEVLAVLEQE PIVLPAWDPM

GALA
Length:454
Mass (Da):52,323
Last modified:February 1, 1997 - v1
Checksum:i30BAC4A1A89DC1DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87336 mRNA Translation: BAA13333.1
PIRiJC4886
UniGeneiRn.100672

Similar proteinsi

Entry informationi

Entry nameiBLMH_RAT
AccessioniPrimary (citable) accession number: P70645
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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