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Protein

Bleomycin hydrolase

Gene

Blmh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity (By similarity). Binds single-stranded DNA with higher affinity than double-stranded DNA. May play an important role in the metabolism of antibiotics.By similarity

Catalytic activityi

Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.

pH dependencei

Optimum pH is 7.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73PROSITE-ProRule annotation1
Active sitei372PROSITE-ProRule annotation1
Active sitei396PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.40. 5301.

Protein family/group databases

MEROPSiC01.084.

Names & Taxonomyi

Protein namesi
Recommended name:
Bleomycin hydrolase (EC:3.4.22.40)
Short name:
BH
Short name:
BLM hydrolase
Short name:
BMH
Gene namesi
Name:Blmh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1304668. Blmh.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000505531 – 454Bleomycin hydrolaseAdd BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei391N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP70645.
PeptideAtlasiP70645.
PRIDEiP70645.

PTM databases

iPTMnetiP70645.
PhosphoSitePlusiP70645.

Expressioni

Tissue specificityi

Expressed at relatively higher levels in the stomach, esophagus, spleen, thymus and testis, and at lower levels in the skin, lung and skeletal muscle.

Gene expression databases

BgeeiENSRNOG00000003563.

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

IntActiP70645. 1 interactor.
STRINGi10116.ENSRNOP00000005125.

Structurei

3D structure databases

ProteinModelPortaliP70645.
SMRiP70645.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4128. Eukaryota.
COG3579. LUCA.
HOVERGENiHBG002388.
InParanoidiP70645.
OMAiKLYTVDY.

Family and domain databases

CDDicd00585. Peptidase_C1B. 1 hit.
InterProiIPR000169. Pept_cys_AS.
IPR004134. Peptidase_C1B.
[Graphical view]
PANTHERiPTHR10363. PTHR10363. 1 hit.
PfamiPF03051. Peptidase_C1_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005700. PepC. 1 hit.
PROSITEiPS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70645-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNAGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQGAQ
60 70 80 90 100
HVFQHVVPQE GKPVTNQKSS GRCWIFSCLN VMRLPFMKKF NIEEFEFSQS
110 120 130 140 150
YLFFWDKVER CYFFLNAFVD TAQKKEPEDG RLVQYLLMNP TNDGGQWDML
160 170 180 190 200
VNIVEKYGVV PKKCFPESHT TEATRRMNDI LNHKMREFCI RLRNLVHSGA
210 220 230 240 250
TKGEISSTQD AMMEEIFRVV CICLGNPPET FTWEYRDKDK NYHKVGPITP
260 270 280 290 300
LQFYKEHVKP LFNMEDKICF VNDPRPQHKY NKLYTVDYLS NMVGGRKTLY
310 320 330 340 350
NNQPIDFLKK MVAASIRDGE AVWFGCDVGK HFNGKLGLSD MNVYDHELVF
360 370 380 390 400
GVSLKNMNKA ERLAFGESLM THAMTFTAVS EKDDQEGAFV KWRVENSWGE
410 420 430 440 450
DHGHKGYLCM TDEWFSEYVY EVVVDKKHVP EEVLAVLEQE PIVLPAWDPM

GALA
Length:454
Mass (Da):52,323
Last modified:February 1, 1997 - v1
Checksum:i30BAC4A1A89DC1DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87336 mRNA. Translation: BAA13333.1.
PIRiJC4886.
UniGeneiRn.100672.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87336 mRNA. Translation: BAA13333.1.
PIRiJC4886.
UniGeneiRn.100672.

3D structure databases

ProteinModelPortaliP70645.
SMRiP70645.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP70645. 1 interactor.
STRINGi10116.ENSRNOP00000005125.

Protein family/group databases

MEROPSiC01.084.

PTM databases

iPTMnetiP70645.
PhosphoSitePlusiP70645.

Proteomic databases

PaxDbiP70645.
PeptideAtlasiP70645.
PRIDEiP70645.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi1304668. Blmh.

Phylogenomic databases

eggNOGiKOG4128. Eukaryota.
COG3579. LUCA.
HOVERGENiHBG002388.
InParanoidiP70645.
OMAiKLYTVDY.

Enzyme and pathway databases

BRENDAi3.4.22.40. 5301.

Miscellaneous databases

PROiP70645.

Gene expression databases

BgeeiENSRNOG00000003563.

Family and domain databases

CDDicd00585. Peptidase_C1B. 1 hit.
InterProiIPR000169. Pept_cys_AS.
IPR004134. Peptidase_C1B.
[Graphical view]
PANTHERiPTHR10363. PTHR10363. 1 hit.
PfamiPF03051. Peptidase_C1_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005700. PepC. 1 hit.
PROSITEiPS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLMH_RAT
AccessioniPrimary (citable) accession number: P70645
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.