P70645 (BLMH_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bleomycin hydrolase
Short name=BH
Short name=BLM hydrolase
Short name=BMH
EC=3.4.22.40

Gene names

Name:

Blmh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity By similarity. Binds single-stranded DNA with higher affinity than double-stranded DNA. May play an important role in the metabolism of antibiotics.
Catalytic activity	Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.
Subunit structure	Homohexamer.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed at relatively higher levels in the stomach, esophagus, spleen, thymus and testis, and at lower levels in the skin, lung and skeletal muscle.
Sequence similarities	Belongs to the peptidase C1 family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.5.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Bleomycin hydrolase		PRO_0000050553
Sites
	Active site	73	1	By similarity
	Active site	372	1	By similarity
	Active site	396	1	By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.2
	Modified residue	391	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70645 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 30BAC4A1A89DC1DC	FASTA	454	52,323
	10 20 30 40 50 60 MNNAGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQGAQ HVFQHVVPQE 70 80 90 100 110 120 GKPVTNQKSS GRCWIFSCLN VMRLPFMKKF NIEEFEFSQS YLFFWDKVER CYFFLNAFVD 130 140 150 160 170 180 TAQKKEPEDG RLVQYLLMNP TNDGGQWDML VNIVEKYGVV PKKCFPESHT TEATRRMNDI 190 200 210 220 230 240 LNHKMREFCI RLRNLVHSGA TKGEISSTQD AMMEEIFRVV CICLGNPPET FTWEYRDKDK 250 260 270 280 290 300 NYHKVGPITP LQFYKEHVKP LFNMEDKICF VNDPRPQHKY NKLYTVDYLS NMVGGRKTLY 310 320 330 340 350 360 NNQPIDFLKK MVAASIRDGE AVWFGCDVGK HFNGKLGLSD MNVYDHELVF GVSLKNMNKA 370 380 390 400 410 420 ERLAFGESLM THAMTFTAVS EKDDQEGAFV KWRVENSWGE DHGHKGYLCM TDEWFSEYVY 430 440 450 EVVVDKKHVP EEVLAVLEQE PIVLPAWDPM GALA

References

[1]	"Cloning and analysis of cDNA encoding rat bleomycin hydrolase, a DNA-binding cysteine protease." Takeda A., Masuda Y., Yamamoto T., Hirabayashi T., Nakamura Y., Nakaya K. J. Biochem. 120:353-359(1996) [PubMed: 8889821] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 44-62; 165-175 AND 283-296. Tissue: Spleen.
[2]	"Purification and characterization of bleomycin hydrolase, which represents a new family of cysteine proteases, from rat skin." Takeda A., Higuchi D., Yamamoto T., Nakamura Y., Masuda Y., Hirabayashi T., Nakaya K. J. Biochem. 119:29-36(1996) [PubMed: 8907172] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10, ACETYLATION AT MET-1, CHARACTERIZATION. Tissue: Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87336 mRNA. Translation: BAA13333.1.
IPI	IPI00231419.
PIR	JC4886.
UniGene	Rn.100672.
3D structure databases
ProteinModelPortal	P70645.
SMR	P70645. Positions 2-454.
ModBase	Search...
Protein-protein interaction databases
IntAct	P70645. 1 interaction.
STRING	P70645.
Protein family/group databases
MEROPS	C01.084.
Proteomic databases
PRIDE	P70645.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	NM_001034163. rat.
Organism-specific databases
RGD	1304668. Blmh.
Phylogenomic databases
GeneTree	ENSGT00390000001735.
HOVERGEN	HBG002388.
InParanoid	P70645.
OrthoDB	EOG4J3WGT.
Enzyme and pathway databases
BRENDA	3.4.22.40. 5301.
Gene expression databases
ArrayExpress	P70645.
Genevestigator	P70645.
GermOnline	ENSRNOG00000003563. Rattus norvegicus.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR004134. Peptidase_C1B. [Graphical view]
PANTHER	PTHR10363. Peptidase_C1B. 1 hit.
Pfam	PF03051. Peptidase_C1_2. 1 hit. [Graphical view]
PIRSF	PIRSF005700. PepC. 1 hit.
PROSITE	PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLMH_RAT

Accession

Primary (citable) accession number: P70645

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program