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Protein

Glutamate carboxypeptidase 2

Gene

Folh1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides (By similarity). In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate.By similarity
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.By similarity

Catalytic activityi

Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit. Required for NAALADase activity.

Enzyme regulationi

The NAALADase activity is inhibited by beta-NAAG, quisqualic acid and 2-(phosphonomethyl)glutaric acid (PMG).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei212 – 2121SubstrateBy similarity
Binding sitei259 – 2591SubstrateBy similarity
Metal bindingi271 – 2711CalciumBy similarity
Metal bindingi274 – 2741Calcium; via carbonyl oxygenBy similarity
Metal bindingi379 – 3791Zinc 1; via tele nitrogen; catalyticBy similarity
Metal bindingi389 – 3891Zinc 1; catalyticBy similarity
Metal bindingi389 – 3891Zinc 2By similarity
Active sitei426 – 4261Nucleophile; for NAALADase activityBy similarity
Binding sitei426 – 4261SubstrateBy similarity
Metal bindingi427 – 4271Zinc 2By similarity
Metal bindingi435 – 4351CalciumBy similarity
Metal bindingi438 – 4381CalciumBy similarity
Metal bindingi455 – 4551Zinc 1; catalyticBy similarity
Binding sitei521 – 5211SubstrateBy similarity
Binding sitei554 – 5541SubstrateBy similarity
Metal bindingi555 – 5551Zinc 2; via tele nitrogenBy similarity
Active sitei630 – 6301Charge relay systemSequence analysis
Active sitei668 – 6681Charge relay systemSequence analysis
Active sitei691 – 6911Charge relay systemSequence analysis

GO - Molecular functioni

GO - Biological processi

  • positive regulation of apoptotic process Source: RGD
  • proteolysis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Dipeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.21. 5301.

Protein family/group databases

MEROPSiM28.010.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate carboxypeptidase 2 (EC:3.4.17.21)
Alternative name(s):
Folate hydrolase 1
Folylpoly-gamma-glutamate carboxypeptidase
Short name:
FGCP
Glutamate carboxypeptidase II
Short name:
GCPII
Membrane glutamate carboxypeptidase
Short name:
mGCP
N-acetylated-alpha-linked acidic dipeptidase I
Short name:
NAALADase I
Prostate-specific membrane antigen homolog
Pteroylpoly-gamma-glutamate carboxypeptidase
Gene namesi
Name:Folh1
Synonyms:Naalad1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70963. Folh1.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type II membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919CytoplasmicSequence analysisAdd
BLAST
Transmembranei20 – 4425Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini45 – 752708ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5098.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 752752Glutamate carboxypeptidase 2PRO_0000174120Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101PhosphoserineCombined sources
Glycosylationi48 – 481N-linked (GlcNAc...)Sequence analysis
Glycosylationi78 – 781N-linked (GlcNAc...)By similarity
Glycosylationi123 – 1231N-linked (GlcNAc...)By similarity
Glycosylationi155 – 1551N-linked (GlcNAc...)By similarity
Glycosylationi197 – 1971N-linked (GlcNAc...)By similarity
Glycosylationi338 – 3381N-linked (GlcNAc...)By similarity
Glycosylationi461 – 4611N-linked (GlcNAc...)By similarity
Glycosylationi478 – 4781N-linked (GlcNAc...)By similarity
Glycosylationi615 – 6151N-linked (GlcNAc...)Sequence analysis
Glycosylationi640 – 6401N-linked (GlcNAc...)By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP70627.
PRIDEiP70627.

PTM databases

iPTMnetiP70627.
PhosphoSiteiP70627.
UniCarbKBiP70627.

Expressioni

Tissue specificityi

Widely expressed throughout brain regions with highest levels in the hippocampus, dentate gyrus, priform cortex, choroid plexus of ventricles, pineal gland, anterior lobe of the pituitary gland and supraoptic nucleus. High levels also found in the cerebral cortex, substantia nigra, pontine nucleus and the granule cell layer of cerebellum. Highly expressed in astrocytes and non-myelinating Schwann cells. Also expressed in kidney, localizing to the proximal brush border of the renal tube.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018592.

Chemistry

BindingDBiP70627.

Structurei

3D structure databases

ProteinModelPortaliP70627.
SMRiP70627. Positions 61-751.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni276 – 589314NAALADaseAdd
BLAST
Regioni519 – 5202Substrate bindingBy similarity
Regioni536 – 5383Substrate bindingBy similarity
Regioni554 – 5552Substrate bindingBy similarity
Regioni701 – 7022Substrate bindingBy similarity

Domaini

The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.

Sequence similaritiesi

Belongs to the peptidase M28 family. M28B subfamily.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2195. Eukaryota.
COG2234. LUCA.
HOGENOMiHOG000211921.
HOVERGENiHBG051639.
InParanoidiP70627.
KOiK14592.
PhylomeDBiP70627.

Family and domain databases

Gene3Di1.20.930.40. 1 hit.
InterProiIPR003137. PA_domain.
IPR007484. Peptidase_M28.
IPR007365. TFR-like_dimer_dom.
[Graphical view]
PfamiPF02225. PA. 1 hit.
PF04389. Peptidase_M28. 1 hit.
PF04253. TFR_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF47672. SSF47672. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: P70627-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWNAQQDSDS AEALGRRQRW FCAGTLVLAF TGTFIIGFLF GWFIKPSNDS
60 70 80 90 100
TSSVSYPGMK KAFLQELKAE NIKKFLYNFT RTPHLAGTQH NFELAKQIHA
110 120 130 140 150
QWKEFGLDLV ELSDYDVLLS YPNKTHPNYI SIINEDGNEI FKTSLAELSP
160 170 180 190 200
PGYENISDVV PPYSAFSPQG TPEGDLVYVN YARTEDFFKL ERVMKINCSG
210 220 230 240 250
KIVIARYGQV FRGNKVKNAQ LAGAKGIILY SDPADYFVPG VKSYPDGWNL
260 270 280 290 300
PGGGVQRGNV LNLNGAGDPL TPGYPANEYA YRHEFTEAVG LPSIPVHPIG
310 320 330 340 350
YDDAQKLLEH MGGSAPPDSS WKGGLKVPYN VGPGFAGNFS KQKVKLHIHS
360 370 380 390 400
YNKVTRIYNV IGTLKGAVEP DRYVILGGHR DAWVFGGIDP QSGAAVVHEI
410 420 430 440 450
VRTFGTLKKK GWRPRRTILF ASWDAEEFGL LGSTEWAEEH SRLLQERGVA
460 470 480 490 500
YINADSSIEG NYTLRVDCTP LMHSLVYNLT KELPSPDEGF EGKSLYDSWK
510 520 530 540 550
EKSPSTEFIG MPRISKLGSG NDFEVFFQRL GIASGRARYT KNWKNNKVSS
560 570 580 590 600
YPLYHSVYET YELVEKFYDP TFKYHLTVAQ VRGAMVFELA NSIVLPFDCQ
610 620 630 640 650
SYAVALKKHA ETIYNISMNH PQEMKAYMIS FDSLFSAVNN FTDVASKFNQ
660 670 680 690 700
RLQDLDKSNP ILLRILNDQL MYLERAFIDP LGLPGRPFYR HIIYAPSSHN
710 720 730 740 750
KYAGESFPGI YDALFDINNK VDTSKAWREV KRQISIAAFT VQAAAETLRE

VD
Length:752
Mass (Da):84,540
Last modified:February 1, 1997 - v1
Checksum:i5C0915A3B9C71E41
GO
Isoform 2 (identifier: P70627-2)
Also known as: Short form
Sequence is not available
Note: Probably inactive.
Length:
Mass (Da):
Isoform 3 (identifier: P70627-3)
Also known as: Long form
Sequence is not available
Length:
Mass (Da):

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75973 mRNA. Translation: AAC53423.1.
AF040256 mRNA. Translation: AAC40067.1.
AF513486 mRNA. Translation: AAM47015.1.
AF039707 mRNA. Translation: AAB96759.1.
RefSeqiNP_476533.1. NM_057185.2. [P70627-1]
UniGeneiRn.89278.

Genome annotation databases

GeneIDi85309.
KEGGirno:85309.
UCSCiRGD:70963. rat. [P70627-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75973 mRNA. Translation: AAC53423.1.
AF040256 mRNA. Translation: AAC40067.1.
AF513486 mRNA. Translation: AAM47015.1.
AF039707 mRNA. Translation: AAB96759.1.
RefSeqiNP_476533.1. NM_057185.2. [P70627-1]
UniGeneiRn.89278.

3D structure databases

ProteinModelPortaliP70627.
SMRiP70627. Positions 61-751.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018592.

Chemistry

BindingDBiP70627.
ChEMBLiCHEMBL5098.

Protein family/group databases

MEROPSiM28.010.

PTM databases

iPTMnetiP70627.
PhosphoSiteiP70627.
UniCarbKBiP70627.

Proteomic databases

PaxDbiP70627.
PRIDEiP70627.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi85309.
KEGGirno:85309.
UCSCiRGD:70963. rat. [P70627-1]

Organism-specific databases

CTDi2346.
RGDi70963. Folh1.

Phylogenomic databases

eggNOGiKOG2195. Eukaryota.
COG2234. LUCA.
HOGENOMiHOG000211921.
HOVERGENiHBG051639.
InParanoidiP70627.
KOiK14592.
PhylomeDBiP70627.

Enzyme and pathway databases

BRENDAi3.4.17.21. 5301.

Miscellaneous databases

PROiP70627.

Family and domain databases

Gene3Di1.20.930.40. 1 hit.
InterProiIPR003137. PA_domain.
IPR007484. Peptidase_M28.
IPR007365. TFR-like_dimer_dom.
[Graphical view]
PfamiPF02225. PA. 1 hit.
PF04389. Peptidase_M28. 1 hit.
PF04253. TFR_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF47672. SSF47672. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFOLH1_RAT
AccessioniPrimary (citable) accession number: P70627
Secondary accession number(s): Q547B6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: February 1, 1997
Last modified: June 8, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.