Reviewed, UniProtKB/Swiss-Prot P70619 (GSHR_RAT)

Names and origin

Protein names

Recommended name:
    Glutathione reductase
      Short name=GRase
      Short name=GR
    EC=1.8.1.7

Gene names

Name:

Gsr

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	424 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol By similarity.
Catalytic activity	2 glutathione + NADP+ = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer; disulfide-linked By similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Sequence caution	The sequence AAB18132.1 differs from that shown. Reason: Frameshift at position 7.

Ontologies

Keywords
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Inferred from mutant phenotype. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD mitochondrion Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	FAD binding Inferred from direct assay. Source: RGD NADP or NADPH binding Inferred from direct assay. Source: RGD glutathione binding Inferred from direct assay. Source: RGD glutathione-disulfide reductase activity Inferred from direct assay. Source: RGD protein homodimerization activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 424	›424	Glutathione reductase		PRO_0000067957
Sites
	Active site	413	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	32		Interchain By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P70619-1 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: 0714FF531F90BEBA	FASTA	424	46,301
	10 20 30 40 50 60 VNVGCVPKKV MWNTAVHSEF IHDHVDYGFQ NCKSKFNWHV IKEKRDAYVS RLNNIYQNNL 70 80 90 100 110 120 TKSHIEVIHG YATFRDGPQP TAEVNGKKFT APHILIATGG VPTVPHENQI PGASLGITSD 130 140 150 160 170 180 GFFQLEDLPS RSVIVGAGYI AVEIAGILSA LGSKTSLMIR HDKVLRSFDS LISSNCTEEL 190 200 210 220 230 240 ENAGGVEVLT VKKFSQVKEV KKTSSGLELH VVTALPGRKP TVTTIPDVDC LLWAIGRDPN 250 260 270 280 290 300 SKGLNLNKLG IQTDDKGHIL VDEFQNTNVK GVYAVGDVCG KALLTPVAIA AGRKLAHRLF 310 320 330 340 350 360 EGKEDSRLDY DNIPTVVFSH PPIGTVGLTE DEAVHKYGKD NVKIYSTAFT PMYHAVTTRK 370 380 390 400 410 420 TKCVMKMVCA NKEEKVVGIH MQGIGCDEML QGFAVAVKMG ATKADFDNRV AIHPTSSEEL VTLR

References

[1]	Hou W.M., Cheplinsky A.B. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U73174 mRNA. Translation: AAB18132.1. Frameshift.
IPI	IPI00190531.
RefSeq	NP_446358.1.
UniGene	Rn.19721
3D structure databases
HSSP	HSSP built from PDB template 1ALG based on UniProtKB P00390.
SMR	P70619. Positions 1-424.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000014915. Rattus norvegicus. [Contig view]
GeneID	116686.
KEGG	rno:116686.
Organism-specific databases
RGD	621747. Gsr.
Phylogenomic databases
HOVERGEN	P70619.
Enzyme and pathway databases
BRENDA	1.8.1.7. 248.
Gene expression databases
ArrayExpress	P70619.
GermOnline	ENSRNOG00000014915. Rattus norvegicus.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006322. Glut_reduct_1. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01421. gluta_reduc_1. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. Partial match. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	619544.

Entry information

Entry name

GSHR_RAT

Accession

Primary (citable) accession number: P70619

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)