Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Double C2-like domain-containing protein alpha

Gene

Doc2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca2+-dependent secretory lysosome exocytosis in mast cells (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • regulation of calcium ion-dependent exocytosis Source: Ensembl
  • synaptic vesicle exocytosis Source: RGD
Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

Calcium, Calcium/phospholipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Double C2-like domain-containing protein alpha
Short name:
Doc2-alpha
Gene namesi
Name:Doc2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620518. Doc2a.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • lysosome Source: UniProtKB
  • neuron projection Source: UniProtKB-SubCell
  • synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Lysosome, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Double C2-like domain-containing protein alphaPRO_0000079967Add
BLAST

Proteomic databases

PaxDbiP70611.
PRIDEiP70611.

PTM databases

iPTMnetiP70611.
PhosphoSiteiP70611.

Expressioni

Tissue specificityi

Predominantly expressed in brain. Also found in non-neural tissues. Expressed in RBL-2H3 mast cell line.2 Publications

Interactioni

Subunit structurei

Interacts (via N-terminus) with UNC13A. Interacts with cytoplasmic dynein light chain DYNLT1. Interacts with UNC13D (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027022.

Structurei

3D structure databases

ProteinModelPortaliP70611.
SMRiP70611. Positions 93-218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini94 – 198105C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini256 – 359104C2 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9292Interaction with UNC13D and DYNLT1By similarityAdd
BLAST
Regioni218 – 403186Interaction with UNC13DBy similarityAdd
BLAST

Domaini

C2 domain 1 is involved in binding calcium and phospholipids.By similarity

Sequence similaritiesi

Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410INTM. Eukaryota.
ENOG410YZ99. LUCA.
GeneTreeiENSGT00810000125376.
HOGENOMiHOG000232127.
HOVERGENiHBG051388.
InParanoidiP70611.
KOiK19916.
OMAiWNEDLMY.
OrthoDBiEOG7CZK67.
PhylomeDBiP70611.
TreeFamiTF351844.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR014638. Doc2.
IPR030535. Doc2a.
IPR001565. Synaptotagmin.
[Graphical view]
PANTHERiPTHR10024:SF233. PTHR10024:SF233. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PIRSFiPIRSF036931. Doc2. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGRRGDRMT INIQEHMAIN VCPGPIRPIR QISDYFPRRG PGPEGGGGGR
60 70 80 90 100
GFGEAPAHLA PLALAPPAAL LGATTPDDGA EVDSYDSDDT TALGTLEFDL
110 120 130 140 150
LYDQASCMLH CRILRAKGLK PMDFNGLADP YVKLHLLPGA CKANKLKTKT
160 170 180 190 200
QRNTLNPVWN EELTYSGITD DDITHKVLRI SVCDEDKLSH NEFIGEIRVP
210 220 230 240 250
LRRLKPSQKK HFNICLERQV PLPSPSSMSA ALRGISCYLK ELEQAEQGPG
260 270 280 290 300
LLEERGRILL SLSYSSRRHG LLVGIVRCAH LAAMDVNGYS DPYVKTYLRP
310 320 330 340 350
DVDKKSKHKT CVKKKTLNPE FNEEFFYEME LSTLATKTLE VTVWDYDIGK
360 370 380 390 400
SNDFIGGVSL GPGARGEAQK HWRDCLHQPD TAVERWHTLT SELPPAAGAL

PLA
Length:403
Mass (Da):44,590
Last modified:February 1, 1997 - v1
Checksum:i24EECA95486FF673
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70779 mRNA. Translation: AAB47748.1.
RefSeqiNP_075226.1. NM_022937.2.
XP_006230397.1. XM_006230335.2.
XP_006230398.1. XM_006230336.1.
XP_006230399.1. XM_006230337.2.
XP_006230401.1. XM_006230339.2.
XP_006230402.1. XM_006230340.1.
XP_006230403.1. XM_006230341.2.
XP_008758140.1. XM_008759918.1.
UniGeneiRn.220257.

Genome annotation databases

EnsembliENSRNOT00000027022; ENSRNOP00000027022; ENSRNOG00000019920.
GeneIDi65031.
KEGGirno:65031.
UCSCiRGD:620518. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70779 mRNA. Translation: AAB47748.1.
RefSeqiNP_075226.1. NM_022937.2.
XP_006230397.1. XM_006230335.2.
XP_006230398.1. XM_006230336.1.
XP_006230399.1. XM_006230337.2.
XP_006230401.1. XM_006230339.2.
XP_006230402.1. XM_006230340.1.
XP_006230403.1. XM_006230341.2.
XP_008758140.1. XM_008759918.1.
UniGeneiRn.220257.

3D structure databases

ProteinModelPortaliP70611.
SMRiP70611. Positions 93-218.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027022.

PTM databases

iPTMnetiP70611.
PhosphoSiteiP70611.

Proteomic databases

PaxDbiP70611.
PRIDEiP70611.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027022; ENSRNOP00000027022; ENSRNOG00000019920.
GeneIDi65031.
KEGGirno:65031.
UCSCiRGD:620518. rat.

Organism-specific databases

CTDi8448.
RGDi620518. Doc2a.

Phylogenomic databases

eggNOGiENOG410INTM. Eukaryota.
ENOG410YZ99. LUCA.
GeneTreeiENSGT00810000125376.
HOGENOMiHOG000232127.
HOVERGENiHBG051388.
InParanoidiP70611.
KOiK19916.
OMAiWNEDLMY.
OrthoDBiEOG7CZK67.
PhylomeDBiP70611.
TreeFamiTF351844.

Miscellaneous databases

PROiP70611.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR014638. Doc2.
IPR030535. Doc2a.
IPR001565. Synaptotagmin.
[Graphical view]
PANTHERiPTHR10024:SF233. PTHR10024:SF233. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PIRSFiPIRSF036931. Doc2. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "DOC2 proteins in rat brain: complementary distribution and proposed function as vesicular adapter proteins in early stages of secretion."
    Verhage M., de Vries K.J., Roeshol H., Burbach J.P., Gispen W.H., Suedhof T.C.
    Neuron 18:453-461(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "Interaction of Doc2 with tctex-1, a light chain of cytoplasmic dynein. Implication in dynein-dependent vesicle transport."
    Nagano F., Orita S., Sasaki T., Naito A., Sakaguchi G., Maeda M., Watanabe T., Kominami E., Uchiyama Y., Takai Y.
    J. Biol. Chem. 273:30065-30068(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DYNLT1.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "Doc2 alpha and Munc13-4 regulate Ca(2+) -dependent secretory lysosome exocytosis in mast cells."
    Higashio H., Nishimura N., Ishizaki H., Miyoshi J., Orita S., Sakane A., Sasaki T.
    J. Immunol. 180:4774-4784(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiDOC2A_RAT
AccessioniPrimary (citable) accession number: P70611
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: February 1, 1997
Last modified: June 8, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.