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Protein

Double C2-like domain-containing protein alpha

Gene

Doc2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca2+-dependent secretory lysosome exocytosis in mast cells (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processExocytosis
LigandCalcium, Calcium/phospholipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Double C2-like domain-containing protein alpha
Short name:
Doc2-alpha
Gene namesi
Name:Doc2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620518 Doc2a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Lysosome, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000799671 – 403Double C2-like domain-containing protein alphaAdd BLAST403

Proteomic databases

PaxDbiP70611
PRIDEiP70611

PTM databases

iPTMnetiP70611
PhosphoSitePlusiP70611

Expressioni

Tissue specificityi

Predominantly expressed in brain. Also found in non-neural tissues. Expressed in RBL-2H3 mast cell line.2 Publications

Gene expression databases

BgeeiENSRNOG00000019920

Interactioni

Subunit structurei

Interacts (via N-terminus) with UNC13A. Interacts with cytoplasmic dynein light chain DYNLT1. Interacts with UNC13D (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027022

Structurei

3D structure databases

ProteinModelPortaliP70611
SMRiP70611
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini94 – 198C2 1PROSITE-ProRule annotationAdd BLAST105
Domaini256 – 359C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 92Interaction with UNC13D and DYNLT1By similarityAdd BLAST92
Regioni218 – 403Interaction with UNC13DBy similarityAdd BLAST186

Domaini

C2 domain 1 is involved in binding calcium and phospholipids.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410INTM Eukaryota
ENOG410YZ99 LUCA
GeneTreeiENSGT00810000125376
HOGENOMiHOG000232127
HOVERGENiHBG051388
InParanoidiP70611
KOiK19916
OMAiKVERWHT
OrthoDBiEOG091G0FPP
PhylomeDBiP70611
TreeFamiTF351844

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR014638 Doc2
IPR030535 Doc2a
IPR001565 Synaptotagmin
PANTHERiPTHR10024:SF233 PTHR10024:SF233, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PIRSFiPIRSF036931 Doc2, 1 hit
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits

Sequencei

Sequence statusi: Complete.

P70611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGRRGDRMT INIQEHMAIN VCPGPIRPIR QISDYFPRRG PGPEGGGGGR
60 70 80 90 100
GFGEAPAHLA PLALAPPAAL LGATTPDDGA EVDSYDSDDT TALGTLEFDL
110 120 130 140 150
LYDQASCMLH CRILRAKGLK PMDFNGLADP YVKLHLLPGA CKANKLKTKT
160 170 180 190 200
QRNTLNPVWN EELTYSGITD DDITHKVLRI SVCDEDKLSH NEFIGEIRVP
210 220 230 240 250
LRRLKPSQKK HFNICLERQV PLPSPSSMSA ALRGISCYLK ELEQAEQGPG
260 270 280 290 300
LLEERGRILL SLSYSSRRHG LLVGIVRCAH LAAMDVNGYS DPYVKTYLRP
310 320 330 340 350
DVDKKSKHKT CVKKKTLNPE FNEEFFYEME LSTLATKTLE VTVWDYDIGK
360 370 380 390 400
SNDFIGGVSL GPGARGEAQK HWRDCLHQPD TAVERWHTLT SELPPAAGAL

PLA
Length:403
Mass (Da):44,590
Last modified:February 1, 1997 - v1
Checksum:i24EECA95486FF673
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70779 mRNA Translation: AAB47748.1
RefSeqiNP_075226.1, NM_022937.2
XP_006230397.1, XM_006230335.3
XP_006230398.1, XM_006230336.1
XP_006230399.1, XM_006230337.3
XP_006230401.1, XM_006230339.3
XP_006230402.1, XM_006230340.1
XP_006230403.1, XM_006230341.3
XP_008758140.1, XM_008759918.2
XP_017445177.1, XM_017589688.1
XP_017445178.1, XM_017589689.1
UniGeneiRn.220257

Genome annotation databases

EnsembliENSRNOT00000027022; ENSRNOP00000027022; ENSRNOG00000019920
GeneIDi65031
KEGGirno:65031
UCSCiRGD:620518 rat

Similar proteinsi

Entry informationi

Entry nameiDOC2A_RAT
AccessioniPrimary (citable) accession number: P70611
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health