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Protein

Small conductance calcium-activated potassium channel protein 3

Gene

Kcnn3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD
  • small conductance calcium-activated potassium channel activity Source: RGD

GO - Biological processi

  • potassium ion transmembrane transport Source: GOC
  • potassium ion transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Small conductance calcium-activated potassium channel protein 3
Short name:
SK3
Short name:
SKCa 3
Short name:
SKCa3
Alternative name(s):
KCa2.3
Gene namesi
Name:Kcnn3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2964. Kcnn3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei289 – 30921Helical; Name=Segment S1Sequence analysisAdd
BLAST
Transmembranei316 – 33621Helical; Name=Segment S2Sequence analysisAdd
BLAST
Transmembranei367 – 38721Helical; Name=Segment S3Sequence analysisAdd
BLAST
Transmembranei406 – 42621Helical; Name=Segment S4Sequence analysisAdd
BLAST
Transmembranei455 – 47521Helical; Name=Segment S5Sequence analysisAdd
BLAST
Intramembranei495 – 51521Pore-forming; Name=Segment H5Sequence analysisAdd
BLAST
Transmembranei524 – 54421Helical; Name=Segment S6Sequence analysisAdd
BLAST

GO - Cellular componenti

  • cell body Source: RGD
  • filopodium Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • neuromuscular junction Source: RGD
  • neuronal cell body Source: RGD
  • neuron projection Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2096673.
GuidetoPHARMACOLOGYi383.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 732732Small conductance calcium-activated potassium channel protein 3PRO_0000155016Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei168 – 1681PhosphoserineBy similarity
Modified residuei310 – 3101PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP70605.

Interactioni

Subunit structurei

Heterooligomer. The complex is composed of 4 channel subunits each of which binds to a calmodulin subunit which regulates the channel activity through calcium-binding (By similarity).By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028117.

Chemistry

BindingDBiP70605.

Structurei

3D structure databases

ProteinModelPortaliP70605.
SMRiP70605. Positions 545-675.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni562 – 63877Calmodulin-bindingBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi19 – 5941Pro-richAdd
BLAST
Compositional biasi30 – 9566Gln-richAdd
BLAST
Compositional biasi684 – 6885Poly-Gln
Compositional biasi728 – 7314Poly-Ser

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3684. Eukaryota.
ENOG410XT9D. LUCA.
HOGENOMiHOG000276908.
HOVERGENiHBG052241.
InParanoidiP70605.
KOiK04944.
PhylomeDBiP70605.

Family and domain databases

InterProiIPR004178. CaM-bd_dom.
IPR015449. K_chnl_Ca-activ_SK.
IPR013099. K_chnl_dom.
[Graphical view]
PANTHERiPTHR10153. PTHR10153. 1 hit.
PfamiPF02888. CaMBD. 1 hit.
PF07885. Ion_trans_2. 1 hit.
PF03530. SK_channel. 1 hit.
[Graphical view]
PRINTSiPR01451. SKCHANNEL.
SMARTiSM01053. CaMBD. 1 hit.
[Graphical view]
SUPFAMiSSF81327. SSF81327. 1 hit.

Sequencei

Sequence statusi: Complete.

P70605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTSGHFHDS GVGDLDEDPK CPCPSSGDEQ QQQQQPPPPS APPAVPQQPP
60 70 80 90 100
GPLLQPQPPQ LQQQQQQQQQ QQQQQQQQQQ APLHPLPQLA QLQSQLVHPG
110 120 130 140 150
LLHSSPTAFR APNSANSTAI LHPSSRQGSQ LNLNDHLLGH SPSSTATSGP
160 170 180 190 200
GGGSRHRQAS PLVHRRDSNP FTEIAMSSCK YSGGVMKPLS RLSASRRNLI
210 220 230 240 250
EAEPEGQPLQ LFSPSNPPEI IISSREDNHA HQTLLHHPNA THNHQHAGTT
260 270 280 290 300
AGSTTFPKAN KRKNQNIGYK LGHRRALFEK RKRLSDYALI FGMFGIVVMV
310 320 330 340 350
IETELSWGLY SKDSMFSLAL KCLISLSTII LLGLIIAYHT REVQLFVIDN
360 370 380 390 400
GADDWRIAMT YERILYISLE MLVCAIHPIP GEYKFFWTAR LAFSYTPSRA
410 420 430 440 450
EADVDIILSI PMFLRLYLIA RVMLLHSKLF TDASSRSIGA LNKINFNTRF
460 470 480 490 500
VMKTLMTICP GTVLLVFSIS LWIIAAWTVR VCERYHDQQD VTSNFLGAMW
510 520 530 540 550
LISITFLSIG YGDMVPHTYC GKGVCLLTGI MGAGCTALVV AVVARKLELT
560 570 580 590 600
KAEKHVHNFM MDTQLTKRIK NAAANVLRET WLIYKHTKLL KKIDHAKVRK
610 620 630 640 650
HQRKFLQAIH QLRGVKMEQR KLSDQANTLV DLSKMQNVMY DLITELNDRS
660 670 680 690 700
EDLEKQIGSL ESKLEHLTAS FNSLPLLIAD TLRQQQQQLL TAFVEARGIS
710 720 730
VAVGTSHAPP SDSPIGISST SFPTPYTSSS SC
Length:732
Mass (Da):81,448
Last modified:November 16, 2001 - v3
Checksum:iC44579B78EFB6ADA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91D → E in AAB81653 (PubMed:8781233).Curated
Sequence conflicti96 – 961L → V in AAB81653 (PubMed:8781233).Curated
Sequence conflicti138 – 1381L → V in AAB81653 (PubMed:8781233).Curated
Sequence conflicti162 – 1621L → V in AAB81653 (PubMed:8781233).Curated
Sequence conflicti190 – 1901S → N in AAB81653 (PubMed:8781233).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti78 – 792Missing .1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69884 mRNA. Translation: AAB81653.1.
AF292389 mRNA. Translation: AAG13967.1.
AF284345 mRNA. Translation: AAG38878.1.
RefSeqiNP_062188.2. NM_019315.2.
UniGeneiRn.10840.

Genome annotation databases

GeneIDi54263.
KEGGirno:54263.
UCSCiRGD:2964. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69884 mRNA. Translation: AAB81653.1.
AF292389 mRNA. Translation: AAG13967.1.
AF284345 mRNA. Translation: AAG38878.1.
RefSeqiNP_062188.2. NM_019315.2.
UniGeneiRn.10840.

3D structure databases

ProteinModelPortaliP70605.
SMRiP70605. Positions 545-675.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028117.

Chemistry

BindingDBiP70605.
ChEMBLiCHEMBL2096673.
GuidetoPHARMACOLOGYi383.

Proteomic databases

PaxDbiP70605.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi54263.
KEGGirno:54263.
UCSCiRGD:2964. rat.

Organism-specific databases

CTDi3782.
RGDi2964. Kcnn3.

Phylogenomic databases

eggNOGiKOG3684. Eukaryota.
ENOG410XT9D. LUCA.
HOGENOMiHOG000276908.
HOVERGENiHBG052241.
InParanoidiP70605.
KOiK04944.
PhylomeDBiP70605.

Miscellaneous databases

PROiP70605.

Family and domain databases

InterProiIPR004178. CaM-bd_dom.
IPR015449. K_chnl_Ca-activ_SK.
IPR013099. K_chnl_dom.
[Graphical view]
PANTHERiPTHR10153. PTHR10153. 1 hit.
PfamiPF02888. CaMBD. 1 hit.
PF07885. Ion_trans_2. 1 hit.
PF03530. SK_channel. 1 hit.
[Graphical view]
PRINTSiPR01451. SKCHANNEL.
SMARTiSM01053. CaMBD. 1 hit.
[Graphical view]
SUPFAMiSSF81327. SSF81327. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Small-conductance, calcium-activated potassium channels from mammalian brain."
    Koehler M., Hirschberg B., Bond C.T., Kinzie J.M., Marrion N.V., Maylie J., Adelman J.P.
    Science 273:1709-1714(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "SK3 is an important component of K(+) channels mediating the afterhyperpolarization in cultured rat SCG neurones."
    Hosseini R., Benton D.C., Dunn P.M., Jenkinson D.H., Moss G.W.
    J. Physiol. (Lond.) 535:323-334(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. "Cloning and functional expression of a liver isoform of the small conductance Ca2+-activated K+ channel SK3."
    Barfod E.T., Moore A.L., Lidofsky S.D.
    Am. J. Physiol. 280:C836-C842(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT 78-GLN-GLN-79 DEL.
    Strain: Sprague-Dawley.
    Tissue: Liver.

Entry informationi

Entry nameiKCNN3_RAT
AccessioniPrimary (citable) accession number: P70605
Secondary accession number(s): Q9EQ81, Q9ERQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 16, 2001
Last modified: June 8, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.