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P70604

- KCNN2_RAT

UniProt

P70604 - KCNN2_RAT

Protein

Small conductance calcium-activated potassium channel protein 2

Gene

Kcnn2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.

    GO - Molecular functioni

    1. calmodulin binding Source: RGD
    2. protein binding Source: IntAct
    3. small conductance calcium-activated potassium channel activity Source: UniProtKB

    GO - Biological processi

    1. potassium ion transmembrane transport Source: GOC
    2. potassium ion transport Source: UniProtKB
    3. regulation of neuronal synaptic plasticity Source: RGD

    Keywords - Molecular functioni

    Ion channel

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Calmodulin-binding

    Enzyme and pathway databases

    ReactomeiREACT_199213. Ca2+ activated K+ channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Small conductance calcium-activated potassium channel protein 2
    Short name:
    SK2
    Short name:
    SKCa 2
    Short name:
    SKCa2
    Alternative name(s):
    KCa2.2
    Gene namesi
    Name:Kcnn2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 18

    Organism-specific databases

    RGDi2963. Kcnn2.

    Subcellular locationi

    GO - Cellular componenti

    1. dendritic spine Source: RGD
    2. integral component of membrane Source: UniProtKB
    3. neuronal cell body Source: RGD
    4. plasma membrane Source: RefGenome

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 580580Small conductance calcium-activated potassium channel protein 2PRO_0000155012Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei161 – 1611Phosphotyrosine1 Publication

    Keywords - PTMi

    Phosphoprotein

    PTM databases

    PhosphoSiteiP70604.

    Expressioni

    Tissue specificityi

    Brain.

    Gene expression databases

    GenevestigatoriP70604.

    Interactioni

    Subunit structurei

    Heterooligomer. The complex is composed of 4 channel subunits each of which binds to a calmodulin subunit which regulates the channel activity through calcium-binding.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Calm3P621612EBI-1031103,EBI-397530

    Protein-protein interaction databases

    DIPiDIP-35337N.
    IntActiP70604. 1 interaction.
    MINTiMINT-197486.
    STRINGi10116.ENSRNOP00000022530.

    Structurei

    Secondary structure

    1
    580
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi398 – 4014
    Helixi414 – 43926
    Helixi446 – 48540
    Helixi489 – 52335

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G4YX-ray1.60B396-487[»]
    1KKDNMR-A396-487[»]
    1QX7X-ray3.09D411-487[»]
    2PNVX-ray2.10A/B488-526[»]
    3SJQX-ray1.90C/D412-487[»]
    4G27X-ray1.65B396-487[»]
    4G28X-ray1.63B396-487[»]
    4J9YX-ray1.51B396-487[»]
    4J9ZX-ray1.66B396-487[»]
    ProteinModelPortaliP70604.
    SMRiP70604. Positions 396-526.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP70604.

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei346 – 36621Pore-forming; Name=Segment H5Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei140 – 16021Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei169 – 18921Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei215 – 23521Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei257 – 27721Helical; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei306 – 32621Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei375 – 39521Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni413 – 48977Calmodulin-bindingAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi42 – 465Poly-Gly
    Compositional biasi52 – 565Poly-Ala
    Compositional biasi80 – 856Poly-Gly
    Compositional biasi88 – 10316Poly-GlyAdd
    BLAST
    Compositional biasi564 – 5674Poly-Arg

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG320393.
    GeneTreeiENSGT00500000044784.
    HOGENOMiHOG000276908.
    HOVERGENiHBG052241.
    InParanoidiP70604.
    KOiK04943.
    PhylomeDBiP70604.
    TreeFamiTF315015.

    Family and domain databases

    InterProiIPR013099. 2pore_dom_K_chnl_dom.
    IPR004178. CaM-bd_dom.
    IPR015449. K_chnl_Ca-activ_SK.
    [Graphical view]
    PANTHERiPTHR10153. PTHR10153. 1 hit.
    PfamiPF02888. CaMBD. 1 hit.
    PF07885. Ion_trans_2. 1 hit.
    PF03530. SK_channel. 1 hit.
    [Graphical view]
    PRINTSiPR01451. SKCHANNEL.
    SMARTiSM01053. CaMBD. 1 hit.
    [Graphical view]
    SUPFAMiSSF81327. SSF81327. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P70604-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSCRYNGGV MRPLSNLSSS RRNLHEMDSE AQPLQPPASV VGGGGGASSP    50
    SAAAAASSSA PEIVVSKPEH NNSNNLALYG TGGGGSTGGG GGGGGGGGGS 100
    GHGSSSGTKS SKKKNQNIGY KLGHRRALFE KRKRLSDYAL IFGMFGIVVM 150
    VIETELSWGA YDKASLYSLA LKCLISLSTI ILLGLIIVYH AREIQLFMVD 200
    NGADDWRIAM TYERIFFICL EILVCAIHPI PGNYTFTWTA RLAFSYAPST 250
    TTADVDIILS IPMFLRLYLI ARVMLLHSKL FTDASSRSIG ALNKINFNTR 300
    FVMKTLMTIC PGTVLLVFSI SLWIIAAWTV RACERYHDQQ DVTSNFLGAM 350
    WLISITFLSI GYGDMVPNTY CGKGVCLLTG IMGAGCTALV VAVVARKLEL 400
    TKAEKHVHNF MMDTQLTKRV KNAAANVLRE TWLIYKNTKL VKKIDHAKVR 450
    KHQRKFLQAI HQLRSVKMEQ RKLNDQANTL VDLAKTQNIM YDMISDLNER 500
    SEDFEKRIVT LETKLETLIG SIHALPGLIS QTIRQQQRDF IETQMENYDK 550
    HVTYNAERSR SSSRRRRSSS TAPPTSSESS 580
    Length:580
    Mass (Da):63,848
    Last modified:February 1, 1997 - v1
    Checksum:iF71E0DAF7EEFA8D4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U69882 mRNA. Translation: AAB09563.1.
    RefSeqiNP_062187.1. NM_019314.1.
    UniGeneiRn.44421.

    Genome annotation databases

    EnsembliENSRNOT00000022530; ENSRNOP00000022530; ENSRNOG00000016675.
    GeneIDi54262.
    KEGGirno:54262.
    UCSCiRGD:2963. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U69882 mRNA. Translation: AAB09563.1 .
    RefSeqi NP_062187.1. NM_019314.1.
    UniGenei Rn.44421.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1G4Y X-ray 1.60 B 396-487 [» ]
    1KKD NMR - A 396-487 [» ]
    1QX7 X-ray 3.09 D 411-487 [» ]
    2PNV X-ray 2.10 A/B 488-526 [» ]
    3SJQ X-ray 1.90 C/D 412-487 [» ]
    4G27 X-ray 1.65 B 396-487 [» ]
    4G28 X-ray 1.63 B 396-487 [» ]
    4J9Y X-ray 1.51 B 396-487 [» ]
    4J9Z X-ray 1.66 B 396-487 [» ]
    ProteinModelPortali P70604.
    SMRi P70604. Positions 396-526.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35337N.
    IntActi P70604. 1 interaction.
    MINTi MINT-197486.
    STRINGi 10116.ENSRNOP00000022530.

    Chemistry

    BindingDBi P70604.
    ChEMBLi CHEMBL2547.
    GuidetoPHARMACOLOGYi 382.

    PTM databases

    PhosphoSitei P70604.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000022530 ; ENSRNOP00000022530 ; ENSRNOG00000016675 .
    GeneIDi 54262.
    KEGGi rno:54262.
    UCSCi RGD:2963. rat.

    Organism-specific databases

    CTDi 3781.
    RGDi 2963. Kcnn2.

    Phylogenomic databases

    eggNOGi NOG320393.
    GeneTreei ENSGT00500000044784.
    HOGENOMi HOG000276908.
    HOVERGENi HBG052241.
    InParanoidi P70604.
    KOi K04943.
    PhylomeDBi P70604.
    TreeFami TF315015.

    Enzyme and pathway databases

    Reactomei REACT_199213. Ca2+ activated K+ channels.

    Miscellaneous databases

    EvolutionaryTracei P70604.
    NextBioi 610790.
    PROi P70604.

    Gene expression databases

    Genevestigatori P70604.

    Family and domain databases

    InterProi IPR013099. 2pore_dom_K_chnl_dom.
    IPR004178. CaM-bd_dom.
    IPR015449. K_chnl_Ca-activ_SK.
    [Graphical view ]
    PANTHERi PTHR10153. PTHR10153. 1 hit.
    Pfami PF02888. CaMBD. 1 hit.
    PF07885. Ion_trans_2. 1 hit.
    PF03530. SK_channel. 1 hit.
    [Graphical view ]
    PRINTSi PR01451. SKCHANNEL.
    SMARTi SM01053. CaMBD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81327. SSF81327. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Small-conductance, calcium-activated potassium channels from mammalian brain."
      Koehler M., Hirschberg B., Bond C.T., Kinzie J.M., Marrion N.V., Maylie J., Adelman J.P.
      Science 273:1709-1714(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. "Mechanism of calcium gating in small-conductance calcium-activated potassium channels."
      Xia X.M., Fakler B., Rivard A.F., Wayman G., Johnson-Pais T., Keen J.E., Ishii T., Hirschberg B., Bond C.T., Lutsenko S., Maylie J., Adelman J.P.
      Nature 395:503-507(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CALMODULIN.
    3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
      Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
      Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-161, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    4. "Structure of the gating domain of a Ca2+-activated K+ channel complexed with Ca2+/calmodulin."
      Schumacher M.A., Rivard A.F., Bachinger H.P., Adelman J.P.
      Nature 410:1120-1124(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 395-490 WITH CALMODULIN.

    Entry informationi

    Entry nameiKCNN2_RAT
    AccessioniPrimary (citable) accession number: P70604
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2001
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3