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P70604

- KCNN2_RAT

UniProt

P70604 - KCNN2_RAT

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Protein

Small conductance calcium-activated potassium channel protein 2

Gene
Kcnn2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.

GO - Molecular functioni

  1. calmodulin binding Source: RGD
  2. protein binding Source: IntAct
  3. small conductance calcium-activated potassium channel activity Source: UniProtKB

GO - Biological processi

  1. potassium ion transmembrane transport Source: GOC
  2. potassium ion transport Source: UniProtKB
  3. regulation of neuronal synaptic plasticity Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

ReactomeiREACT_199213. Ca2+ activated K+ channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Small conductance calcium-activated potassium channel protein 2
Short name:
SK2
Short name:
SKCa 2
Short name:
SKCa2
Alternative name(s):
KCa2.2
Gene namesi
Name:Kcnn2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 18

Organism-specific databases

RGDi2963. Kcnn2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei140 – 16021Helical; Name=Segment S1; Reviewed predictionAdd
BLAST
Transmembranei169 – 18921Helical; Name=Segment S2; Reviewed predictionAdd
BLAST
Transmembranei215 – 23521Helical; Name=Segment S3; Reviewed predictionAdd
BLAST
Transmembranei257 – 27721Helical; Name=Segment S4; Reviewed predictionAdd
BLAST
Transmembranei306 – 32621Helical; Name=Segment S5; Reviewed predictionAdd
BLAST
Intramembranei346 – 36621Pore-forming; Name=Segment H5; Reviewed predictionAdd
BLAST
Transmembranei375 – 39521Helical; Name=Segment S6; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. dendritic spine Source: RGD
  2. integral component of membrane Source: UniProtKB
  3. neuronal cell body Source: RGD
  4. plasma membrane Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Small conductance calcium-activated potassium channel protein 2PRO_0000155012Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei161 – 1611Phosphotyrosine1 Publication

Keywords - PTMi

Phosphoprotein

PTM databases

PhosphoSiteiP70604.

Expressioni

Tissue specificityi

Brain.

Gene expression databases

GenevestigatoriP70604.

Interactioni

Subunit structurei

Heterooligomer. The complex is composed of 4 channel subunits each of which binds to a calmodulin subunit which regulates the channel activity through calcium-binding.

Binary interactionsi

WithEntry#Exp.IntActNotes
Calm3P621612EBI-1031103,EBI-397530

Protein-protein interaction databases

DIPiDIP-35337N.
IntActiP70604. 1 interaction.
MINTiMINT-197486.
STRINGi10116.ENSRNOP00000022530.

Structurei

Secondary structure

1
580
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi398 – 4014
Helixi414 – 43926
Helixi446 – 48540
Helixi489 – 52335

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G4YX-ray1.60B396-487[»]
1KKDNMR-A396-487[»]
1QX7X-ray3.09D411-487[»]
2PNVX-ray2.10A/B488-526[»]
3SJQX-ray1.90C/D412-487[»]
4G27X-ray1.65B396-487[»]
4G28X-ray1.63B396-487[»]
4J9YX-ray1.51B396-487[»]
4J9ZX-ray1.66B396-487[»]
ProteinModelPortaliP70604.
SMRiP70604. Positions 396-526.

Miscellaneous databases

EvolutionaryTraceiP70604.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni413 – 48977Calmodulin-bindingAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi42 – 465Poly-Gly
Compositional biasi52 – 565Poly-Ala
Compositional biasi80 – 856Poly-Gly
Compositional biasi88 – 10316Poly-GlyAdd
BLAST
Compositional biasi564 – 5674Poly-Arg

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG320393.
GeneTreeiENSGT00500000044784.
HOGENOMiHOG000276908.
HOVERGENiHBG052241.
InParanoidiP70604.
KOiK04943.
PhylomeDBiP70604.
TreeFamiTF315015.

Family and domain databases

InterProiIPR013099. 2pore_dom_K_chnl_dom.
IPR004178. CaM-bd_dom.
IPR015449. K_chnl_Ca-activ_SK.
[Graphical view]
PANTHERiPTHR10153. PTHR10153. 1 hit.
PfamiPF02888. CaMBD. 1 hit.
PF07885. Ion_trans_2. 1 hit.
PF03530. SK_channel. 1 hit.
[Graphical view]
PRINTSiPR01451. SKCHANNEL.
SMARTiSM01053. CaMBD. 1 hit.
[Graphical view]
SUPFAMiSSF81327. SSF81327. 1 hit.

Sequencei

Sequence statusi: Complete.

P70604-1 [UniParc]FASTAAdd to Basket

« Hide

MSSCRYNGGV MRPLSNLSSS RRNLHEMDSE AQPLQPPASV VGGGGGASSP    50
SAAAAASSSA PEIVVSKPEH NNSNNLALYG TGGGGSTGGG GGGGGGGGGS 100
GHGSSSGTKS SKKKNQNIGY KLGHRRALFE KRKRLSDYAL IFGMFGIVVM 150
VIETELSWGA YDKASLYSLA LKCLISLSTI ILLGLIIVYH AREIQLFMVD 200
NGADDWRIAM TYERIFFICL EILVCAIHPI PGNYTFTWTA RLAFSYAPST 250
TTADVDIILS IPMFLRLYLI ARVMLLHSKL FTDASSRSIG ALNKINFNTR 300
FVMKTLMTIC PGTVLLVFSI SLWIIAAWTV RACERYHDQQ DVTSNFLGAM 350
WLISITFLSI GYGDMVPNTY CGKGVCLLTG IMGAGCTALV VAVVARKLEL 400
TKAEKHVHNF MMDTQLTKRV KNAAANVLRE TWLIYKNTKL VKKIDHAKVR 450
KHQRKFLQAI HQLRSVKMEQ RKLNDQANTL VDLAKTQNIM YDMISDLNER 500
SEDFEKRIVT LETKLETLIG SIHALPGLIS QTIRQQQRDF IETQMENYDK 550
HVTYNAERSR SSSRRRRSSS TAPPTSSESS 580
Length:580
Mass (Da):63,848
Last modified:February 1, 1997 - v1
Checksum:iF71E0DAF7EEFA8D4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U69882 mRNA. Translation: AAB09563.1.
RefSeqiNP_062187.1. NM_019314.1.
UniGeneiRn.44421.

Genome annotation databases

EnsembliENSRNOT00000022530; ENSRNOP00000022530; ENSRNOG00000016675.
GeneIDi54262.
KEGGirno:54262.
UCSCiRGD:2963. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U69882 mRNA. Translation: AAB09563.1 .
RefSeqi NP_062187.1. NM_019314.1.
UniGenei Rn.44421.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G4Y X-ray 1.60 B 396-487 [» ]
1KKD NMR - A 396-487 [» ]
1QX7 X-ray 3.09 D 411-487 [» ]
2PNV X-ray 2.10 A/B 488-526 [» ]
3SJQ X-ray 1.90 C/D 412-487 [» ]
4G27 X-ray 1.65 B 396-487 [» ]
4G28 X-ray 1.63 B 396-487 [» ]
4J9Y X-ray 1.51 B 396-487 [» ]
4J9Z X-ray 1.66 B 396-487 [» ]
ProteinModelPortali P70604.
SMRi P70604. Positions 396-526.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-35337N.
IntActi P70604. 1 interaction.
MINTi MINT-197486.
STRINGi 10116.ENSRNOP00000022530.

Chemistry

BindingDBi P70604.
ChEMBLi CHEMBL2547.
GuidetoPHARMACOLOGYi 382.

PTM databases

PhosphoSitei P70604.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000022530 ; ENSRNOP00000022530 ; ENSRNOG00000016675 .
GeneIDi 54262.
KEGGi rno:54262.
UCSCi RGD:2963. rat.

Organism-specific databases

CTDi 3781.
RGDi 2963. Kcnn2.

Phylogenomic databases

eggNOGi NOG320393.
GeneTreei ENSGT00500000044784.
HOGENOMi HOG000276908.
HOVERGENi HBG052241.
InParanoidi P70604.
KOi K04943.
PhylomeDBi P70604.
TreeFami TF315015.

Enzyme and pathway databases

Reactomei REACT_199213. Ca2+ activated K+ channels.

Miscellaneous databases

EvolutionaryTracei P70604.
NextBioi 610790.
PROi P70604.

Gene expression databases

Genevestigatori P70604.

Family and domain databases

InterProi IPR013099. 2pore_dom_K_chnl_dom.
IPR004178. CaM-bd_dom.
IPR015449. K_chnl_Ca-activ_SK.
[Graphical view ]
PANTHERi PTHR10153. PTHR10153. 1 hit.
Pfami PF02888. CaMBD. 1 hit.
PF07885. Ion_trans_2. 1 hit.
PF03530. SK_channel. 1 hit.
[Graphical view ]
PRINTSi PR01451. SKCHANNEL.
SMARTi SM01053. CaMBD. 1 hit.
[Graphical view ]
SUPFAMi SSF81327. SSF81327. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Small-conductance, calcium-activated potassium channels from mammalian brain."
    Koehler M., Hirschberg B., Bond C.T., Kinzie J.M., Marrion N.V., Maylie J., Adelman J.P.
    Science 273:1709-1714(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Mechanism of calcium gating in small-conductance calcium-activated potassium channels."
    Xia X.M., Fakler B., Rivard A.F., Wayman G., Johnson-Pais T., Keen J.E., Ishii T., Hirschberg B., Bond C.T., Lutsenko S., Maylie J., Adelman J.P.
    Nature 395:503-507(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CALMODULIN.
  3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-161, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Structure of the gating domain of a Ca2+-activated K+ channel complexed with Ca2+/calmodulin."
    Schumacher M.A., Rivard A.F., Bachinger H.P., Adelman J.P.
    Nature 410:1120-1124(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 395-490 WITH CALMODULIN.

Entry informationi

Entry nameiKCNN2_RAT
AccessioniPrimary (citable) accession number: P70604
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: February 1, 1997
Last modified: September 3, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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