ID   PE2R1_RAT               Reviewed;         405 AA.
AC   P70597; P97537;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   24-JAN-2024, entry version 164.
DE   RecName: Full=Prostaglandin E2 receptor EP1 subtype;
DE            Short=PGE receptor EP1 subtype;
DE            Short=PGE2 receptor EP1 subtype;
DE   AltName: Full=Prostanoid EP1 receptor;
GN   Name=Ptger1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley;
RX   PubMed=9537820; DOI=10.1016/s0014-2999(97)01383-6;
RA   Boie Y., Stocco R., Sawyer N., Slipetz D.M., Ungrin M.D.,
RA   Neuschafer-Rube F., Puschel G.P., Metters K.M., Abramovitz M.;
RT   "Molecular cloning and characterization of the four rat prostaglandin E2
RT   prostanoid receptor subtypes.";
RL   Eur. J. Pharmacol. 340:227-241(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING.
RC   STRAIN=Wistar; TISSUE=Uterus;
RX   PubMed=8940129; DOI=10.1074/jbc.271.49.31255;
RA   Okuda-Ashitaka E., Sakamoto K., Ezashi T., Miwa K., Ito S., Hayaishi O.;
RT   "Suppression of prostaglandin E receptor signaling by the variant form of
RT   EP1 subtype.";
RL   J. Biol. Chem. 271:31255-31261(1996).
CC   -!- FUNCTION: Receptor for prostaglandin E2 (PGE2). The activity of this
CC       receptor is mediated by G(q) proteins which activate a
CC       phosphatidylinositol-calcium second messenger system. May play a role
CC       as an important modulator of renal function (By similarity). Implicated
CC       the smooth muscle contractile response to PGE2 in various tissues.
CC       Isoform 1 and isoform 2 have identical ligand binding properties, but
CC       isoform 2 lacks coupling to calcium mobilization and may therefore
CC       attenuate the action of PGE2 on tissues. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=P70597-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=P70597-2; Sequence=VSP_001927, VSP_001928;
CC   -!- TISSUE SPECIFICITY: Highly abundant in kidney and lung. Found in a
CC       lesser extent in spleen, colon, and thymus. Also expressed in uterine
CC       myometrium and endometrium.
CC   -!- PTM: Phosphorylated. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR   EMBL; U68037; AAB07735.1; -; mRNA.
DR   EMBL; D88751; BAA13691.1; -; mRNA.
DR   EMBL; D88752; BAA13692.1; -; mRNA.
DR   RefSeq; NP_001265404.1; NM_001278475.1. [P70597-1]
DR   AlphaFoldDB; P70597; -.
DR   SMR; P70597; -.
DR   BioGRID; 247666; 1.
DR   STRING; 10116.ENSRNOP00000005470; -.
DR   BindingDB; P70597; -.
DR   ChEMBL; CHEMBL5068; -.
DR   DrugCentral; P70597; -.
DR   GuidetoPHARMACOLOGY; 340; -.
DR   GlyCosmos; P70597; 3 sites, No reported glycans.
DR   GlyGen; P70597; 3 sites.
DR   PhosphoSitePlus; P70597; -.
DR   PaxDb; 10116-ENSRNOP00000005470; -.
DR   Ensembl; ENSRNOT00000005470.5; ENSRNOP00000005470.1; ENSRNOG00000004094.5. [P70597-1]
DR   GeneID; 25637; -.
DR   KEGG; rno:25637; -.
DR   AGR; RGD:3434; -.
DR   CTD; 5731; -.
DR   RGD; 3434; Ptger1.
DR   eggNOG; KOG3656; Eukaryota.
DR   GeneTree; ENSGT01030000234559; -.
DR   HOGENOM; CLU_045991_3_0_1; -.
DR   InParanoid; P70597; -.
DR   OrthoDB; 4642085at2759; -.
DR   PhylomeDB; P70597; -.
DR   TreeFam; TF324982; -.
DR   Reactome; R-RNO-391908; Prostanoid ligand receptors.
DR   Reactome; R-RNO-416476; G alpha (q) signalling events.
DR   PRO; PR:P70597; -.
DR   Proteomes; UP000002494; Chromosome 19.
DR   Bgee; ENSRNOG00000004094; Expressed in thymus and 18 other cell types or tissues.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0031748; F:D1 dopamine receptor binding; ISO:RGD.
DR   GO; GO:0004957; F:prostaglandin E receptor activity; IDA:RGD.
DR   GO; GO:0007191; P:adenylate cyclase-activating dopamine receptor signaling pathway; ISO:RGD.
DR   GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR   GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR   GO; GO:0032496; P:response to lipopolysaccharide; ISO:RGD.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   InterPro; IPR008365; Prostanoid_rcpt.
DR   InterPro; IPR001244; Prostglndn_DP_rcpt.
DR   InterPro; IPR000708; Prostglndn_EP1_rcpt.
DR   PANTHER; PTHR11866; G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER; 1.
DR   PANTHER; PTHR11866:SF3; PROSTAGLANDIN E2 RECEPTOR EP1 SUBTYPE; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00428; PROSTAGLNDNR.
DR   PRINTS; PR01788; PROSTANOIDR.
DR   PRINTS; PR00580; PRSTNOIDEP1R.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR   Genevisible; P70597; RN.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell membrane; Disulfide bond;
KW   G-protein coupled receptor; Glycoprotein; Membrane; Phosphoprotein;
KW   Receptor; Reference proteome; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..405
FT                   /note="Prostaglandin E2 receptor EP1 subtype"
FT                   /id="PRO_0000070052"
FT   TOPO_DOM        1..39
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        40..62
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        63..80
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..99
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        100..113
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..135
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        136..157
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..179
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        180..202
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..228
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        229..301
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        302..323
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        324..337
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        338..357
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        358..405
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        7
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        24
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        34
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        112..190
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT   VAR_SEQ         319..366
FT                   /note="LVVLAIGGWNSNSLQRPLFLAVRLASWNQILDPWVYILLRQAMLRQLL ->
FT                   RGAVAPQAKLFSAPSWPLPAKDPACRQKPAPLLSRTLTFFTLFGNLCK (in
FT                   isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_001927"
FT   VAR_SEQ         367..405
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_001928"
SQ   SEQUENCE   405 AA;  43048 MW;  E8312388B619F9EC CRC64;
     MSPYGLNLSL VDEATTCVTP RVPNTSVVLP TGGNGTSPAL PIFSMTLGAV SNVLALALLA
     QVAGRLRRRR STATFLLFVA SLLAIDLAGH VIPGALVLRL YTAGRAPAGG ACHFLGGCMV
     FFGLCPLLLG CGMAVERCVG VTQPLIHAAR VSVARARLAL ALLAAMALAV ALLPLVHVGH
     YELQYPGTWC FISLGPPGGW RQALLAGLFA GLGLAALLAA LVCNTLSGLA LLRARWRRRR
     SRRFRENAGP DDRRRWGSRG LRLASASSAS SITSTTAALR SSRGGGSARR VHAHDVEMVG
     QLVGIMVVSC ICWSPLLVLV VLAIGGWNSN SLQRPLFLAV RLASWNQILD PWVYILLRQA
     MLRQLLRLLP LRVSAKGGPT ELSLTKSAWE ASSLRSSRHS GFSHL
//