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P70597

- PE2R1_RAT

UniProt

P70597 - PE2R1_RAT

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Protein

Prostaglandin E2 receptor EP1 subtype

Gene

Ptger1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(q) proteins which activate a phosphatidylinositol-calcium second messenger system. May play a role as an important modulator of renal function (By similarity). Implicated the smooth muscle contractile response to PGE2 in various tissues. Isoform 1 and isoform 2 have identical ligand binding properties, but isoform 2 lacks coupling to calcium mobilization and may therefore attenuate the action of PGE2 on tissues.By similarity

GO - Molecular functioni

  1. prostaglandin E receptor activity Source: RGD

GO - Biological processi

  1. response to lipopolysaccharide Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin E2 receptor EP1 subtype
Short name:
PGE receptor EP1 subtype
Short name:
PGE2 receptor EP1 subtype
Alternative name(s):
Prostanoid EP1 receptor
Gene namesi
Name:Ptger1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 19

Organism-specific databases

RGDi3434. Ptger1.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 405405Prostaglandin E2 receptor EP1 subtypePRO_0000070052Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi7 – 71N-linked (GlcNAc...)Sequence Analysis
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi112 ↔ 190PROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated.Curated

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP70597.

Expressioni

Tissue specificityi

Highly abundant in kidney and lung. Found in a lesser extent in spleen, colon, and thymus. Also expressed in uterine myometrium and endometrium.

Gene expression databases

GenevestigatoriP70597.

Interactioni

Protein-protein interaction databases

MINTiMINT-189620.
STRINGi10116.ENSRNOP00000005470.

Structurei

3D structure databases

ProteinModelPortaliP70597.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3939ExtracellularSequence AnalysisAdd
BLAST
Topological domaini63 – 8018CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini100 – 11314ExtracellularSequence AnalysisAdd
BLAST
Topological domaini136 – 15722CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini180 – 20223ExtracellularSequence AnalysisAdd
BLAST
Topological domaini229 – 30173CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini324 – 33714ExtracellularSequence AnalysisAdd
BLAST
Topological domaini358 – 40548CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei40 – 6223Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei81 – 9919Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei114 – 13522Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei158 – 17922Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei203 – 22826Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei302 – 32322Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei338 – 35720Helical; Name=7Sequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253446.
GeneTreeiENSGT00760000119188.
HOGENOMiHOG000015238.
HOVERGENiHBG003621.
InParanoidiP70597.
KOiK04258.
OMAiPAGGACH.
OrthoDBiEOG7PP575.
PhylomeDBiP70597.
TreeFamiTF324982.

Family and domain databases

Gene3Di1.20.1070.10. 2 hits.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
IPR000708. Prostglndn_EP1_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 1 hit.
PTHR11866:SF3. PTHR11866:SF3. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00580. PRSTNOIDEP1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P70597-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSPYGLNLSL VDEATTCVTP RVPNTSVVLP TGGNGTSPAL PIFSMTLGAV
60 70 80 90 100
SNVLALALLA QVAGRLRRRR STATFLLFVA SLLAIDLAGH VIPGALVLRL
110 120 130 140 150
YTAGRAPAGG ACHFLGGCMV FFGLCPLLLG CGMAVERCVG VTQPLIHAAR
160 170 180 190 200
VSVARARLAL ALLAAMALAV ALLPLVHVGH YELQYPGTWC FISLGPPGGW
210 220 230 240 250
RQALLAGLFA GLGLAALLAA LVCNTLSGLA LLRARWRRRR SRRFRENAGP
260 270 280 290 300
DDRRRWGSRG LRLASASSAS SITSTTAALR SSRGGGSARR VHAHDVEMVG
310 320 330 340 350
QLVGIMVVSC ICWSPLLVLV VLAIGGWNSN SLQRPLFLAV RLASWNQILD
360 370 380 390 400
PWVYILLRQA MLRQLLRLLP LRVSAKGGPT ELSLTKSAWE ASSLRSSRHS

GFSHL
Length:405
Mass (Da):43,048
Last modified:February 1, 1997 - v1
Checksum:iE8312388B619F9EC
GO
Isoform 2 (identifier: P70597-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-366: LVVLAIGGWN...LRQAMLRQLL → RGAVAPQAKL...FFTLFGNLCK
     367-405: Missing.

Show »
Length:366
Mass (Da):38,498
Checksum:i82ADD2DCB12EB5C0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei319 – 36648LVVLA…LRQLL → RGAVAPQAKLFSAPSWPLPA KDPACRQKPAPLLSRTLTFF TLFGNLCK in isoform 2. CuratedVSP_001927Add
BLAST
Alternative sequencei367 – 40539Missing in isoform 2. CuratedVSP_001928Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U68037 mRNA. Translation: AAB07735.1.
D88751 mRNA. Translation: BAA13691.1.
D88752 mRNA. Translation: BAA13692.1.
RefSeqiNP_001265404.1. NM_001278475.1. [P70597-1]
UniGeneiRn.11423.

Genome annotation databases

EnsembliENSRNOT00000005470; ENSRNOP00000005470; ENSRNOG00000004094. [P70597-1]
GeneIDi25637.
KEGGirno:25637.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U68037 mRNA. Translation: AAB07735.1 .
D88751 mRNA. Translation: BAA13691.1 .
D88752 mRNA. Translation: BAA13692.1 .
RefSeqi NP_001265404.1. NM_001278475.1. [P70597-1 ]
UniGenei Rn.11423.

3D structure databases

ProteinModelPortali P70597.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-189620.
STRINGi 10116.ENSRNOP00000005470.

Chemistry

BindingDBi P70597.
ChEMBLi CHEMBL5068.
GuidetoPHARMACOLOGYi 340.

Protein family/group databases

GPCRDBi Search...

Proteomic databases

PRIDEi P70597.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000005470 ; ENSRNOP00000005470 ; ENSRNOG00000004094 . [P70597-1 ]
GeneIDi 25637.
KEGGi rno:25637.

Organism-specific databases

CTDi 5731.
RGDi 3434. Ptger1.

Phylogenomic databases

eggNOGi NOG253446.
GeneTreei ENSGT00760000119188.
HOGENOMi HOG000015238.
HOVERGENi HBG003621.
InParanoidi P70597.
KOi K04258.
OMAi PAGGACH.
OrthoDBi EOG7PP575.
PhylomeDBi P70597.
TreeFami TF324982.

Miscellaneous databases

NextBioi 607463.
PROi P70597.

Gene expression databases

Genevestigatori P70597.

Family and domain databases

Gene3Di 1.20.1070.10. 2 hits.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
IPR000708. Prostglndn_EP1_rcpt.
[Graphical view ]
PANTHERi PTHR11866. PTHR11866. 1 hit.
PTHR11866:SF3. PTHR11866:SF3. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00580. PRSTNOIDEP1R.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of the four rat prostaglandin E2 prostanoid receptor subtypes."
    Boie Y., Stocco R., Sawyer N., Slipetz D.M., Ungrin M.D., Neuschafer-Rube F., Puschel G.P., Metters K.M., Abramovitz M.
    Eur. J. Pharmacol. 340:227-241(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  2. "Suppression of prostaglandin E receptor signaling by the variant form of EP1 subtype."
    Okuda-Ashitaka E., Sakamoto K., Ezashi T., Miwa K., Ito S., Hayaishi O.
    J. Biol. Chem. 271:31255-31261(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Strain: Wistar.
    Tissue: Uterus.

Entry informationi

Entry nameiPE2R1_RAT
AccessioniPrimary (citable) accession number: P70597
Secondary accession number(s): P97537
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3