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P70597 (PE2R1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prostaglandin E2 receptor EP1 subtype

Short name=PGE receptor EP1 subtype
Short name=PGE2 receptor EP1 subtype
Alternative name(s):
Prostanoid EP1 receptor
Gene names
Name:Ptger1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length405 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(q) proteins which activate a phosphatidylinositol-calcium second messenger system. May play a role as an important modulator of renal function By similarity. Implicated the smooth muscle contractile response to PGE2 in various tissues. Isoform 1 and isoform 2 have identical ligand binding properties, but isoform 2 lacks coupling to calcium mobilization and may therefore attenuate the action of PGE2 on tissues.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Highly abundant in kidney and lung. Found in a lesser extent in spleen, colon, and thymus. Also expressed in uterine myometrium and endometrium.

Post-translational modification

Phosphorylated Potential.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P70597-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P70597-2)

The sequence of this isoform differs from the canonical sequence as follows:
     319-366: LVVLAIGGWN...LRQAMLRQLL → RGAVAPQAKL...FFTLFGNLCK
     367-405: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 405405Prostaglandin E2 receptor EP1 subtype
PRO_0000070052

Regions

Topological domain1 – 3939Extracellular Potential
Transmembrane40 – 6223Helical; Name=1; Potential
Topological domain63 – 8018Cytoplasmic Potential
Transmembrane81 – 9919Helical; Name=2; Potential
Topological domain100 – 11314Extracellular Potential
Transmembrane114 – 13522Helical; Name=3; Potential
Topological domain136 – 15722Cytoplasmic Potential
Transmembrane158 – 17922Helical; Name=4; Potential
Topological domain180 – 20223Extracellular Potential
Transmembrane203 – 22826Helical; Name=5; Potential
Topological domain229 – 30173Cytoplasmic Potential
Transmembrane302 – 32322Helical; Name=6; Potential
Topological domain324 – 33714Extracellular Potential
Transmembrane338 – 35720Helical; Name=7; Potential
Topological domain358 – 40548Cytoplasmic Potential

Amino acid modifications

Glycosylation71N-linked (GlcNAc...) Potential
Glycosylation241N-linked (GlcNAc...) Potential
Glycosylation341N-linked (GlcNAc...) Potential
Disulfide bond112 ↔ 190 By similarity

Natural variations

Alternative sequence319 – 36648LVVLA…LRQLL → RGAVAPQAKLFSAPSWPLPA KDPACRQKPAPLLSRTLTFF TLFGNLCK in isoform 2.
VSP_001927
Alternative sequence367 – 40539Missing in isoform 2.
VSP_001928

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: E8312388B619F9EC

FASTA40543,048
        10         20         30         40         50         60 
MSPYGLNLSL VDEATTCVTP RVPNTSVVLP TGGNGTSPAL PIFSMTLGAV SNVLALALLA 

        70         80         90        100        110        120 
QVAGRLRRRR STATFLLFVA SLLAIDLAGH VIPGALVLRL YTAGRAPAGG ACHFLGGCMV 

       130        140        150        160        170        180 
FFGLCPLLLG CGMAVERCVG VTQPLIHAAR VSVARARLAL ALLAAMALAV ALLPLVHVGH 

       190        200        210        220        230        240 
YELQYPGTWC FISLGPPGGW RQALLAGLFA GLGLAALLAA LVCNTLSGLA LLRARWRRRR 

       250        260        270        280        290        300 
SRRFRENAGP DDRRRWGSRG LRLASASSAS SITSTTAALR SSRGGGSARR VHAHDVEMVG 

       310        320        330        340        350        360 
QLVGIMVVSC ICWSPLLVLV VLAIGGWNSN SLQRPLFLAV RLASWNQILD PWVYILLRQA 

       370        380        390        400 
MLRQLLRLLP LRVSAKGGPT ELSLTKSAWE ASSLRSSRHS GFSHL 

« Hide

Isoform 2 [UniParc].

Checksum: 82ADD2DCB12EB5C0
Show »

FASTA36638,498

References

[1]"Molecular cloning and characterization of the four rat prostaglandin E2 prostanoid receptor subtypes."
Boie Y., Stocco R., Sawyer N., Slipetz D.M., Ungrin M.D., Neuschafer-Rube F., Puschel G.P., Metters K.M., Abramovitz M.
Eur. J. Pharmacol. 340:227-241(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
[2]"Suppression of prostaglandin E receptor signaling by the variant form of EP1 subtype."
Okuda-Ashitaka E., Sakamoto K., Ezashi T., Miwa K., Ito S., Hayaishi O.
J. Biol. Chem. 271:31255-31261(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Strain: Wistar.
Tissue: Uterus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U68037 mRNA. Translation: AAB07735.1.
D88751 mRNA. Translation: BAA13691.1.
D88752 mRNA. Translation: BAA13692.1.
RefSeqNP_001265404.1. NM_001278475.1.
UniGeneRn.11423.

3D structure databases

ProteinModelPortalP70597.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-189620.
STRING10116.ENSRNOP00000005470.

Chemistry

BindingDBP70597.
ChEMBLCHEMBL5068.
GuidetoPHARMACOLOGY340.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PRIDEP70597.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000005470; ENSRNOP00000005470; ENSRNOG00000004094. [P70597-1]
GeneID25637.
KEGGrno:25637.

Organism-specific databases

CTD5731.
RGD3434. Ptger1.

Phylogenomic databases

eggNOGNOG253446.
GeneTreeENSGT00690000102015.
HOGENOMHOG000015238.
HOVERGENHBG003621.
InParanoidP70597.
KOK04258.
OMAPAGGACH.
OrthoDBEOG7PP575.
PhylomeDBP70597.
TreeFamTF324982.

Gene expression databases

GenevestigatorP70597.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
IPR000708. Prostglndn_EP1_rcpt.
[Graphical view]
PANTHERPTHR11866. PTHR11866. 1 hit.
PTHR11866:SF3. PTHR11866:SF3. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00580. PRSTNOIDEP1R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio607463.
PROP70597.

Entry information

Entry namePE2R1_RAT
AccessionPrimary (citable) accession number: P70597
Secondary accession number(s): P97537
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries