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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

Dut

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+By similarity

Pathway:idUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (Dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei151 – 1511Substrate; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

  • dUTP diphosphatase activity Source: RGD
  • magnesium ion binding Source: GO_Central
  • peroxisome proliferator activated receptor binding Source: RGD
  • pyrimidine deoxyribonucleotide binding Source: RGD
  • receptor inhibitor activity Source: RGD

GO - Biological processi

  • dUMP biosynthetic process Source: RGD
  • dUTP catabolic process Source: RGD
  • liver development Source: RGD
  • negative regulation of receptor activity Source: GOC
  • protein homotrimerization Source: RGD
  • regulation of protein heterodimerization activity Source: RGD
  • response to organic cyclic compound Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_332139. Pyrimidine biosynthesis.
UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
PPAR-interacting protein 4
Short name:
PIP4
dUTP pyrophosphatase
Gene namesi
Name:Dut
Synonyms:Dutp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi620849. Dut.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000182926Add
BLAST

Proteomic databases

PaxDbiP70583.
PRIDEiP70583.

PTM databases

PhosphoSiteiP70583.

Expressioni

Tissue specificityi

Expressed in all tissues examined. Higher levels in heart and kidney.

Gene expression databases

GenevisibleiP70583. RN.

Interactioni

Protein-protein interaction databases

BioGridi269088. 4 interactions.
STRINGi10116.ENSRNOP00000009549.

Structurei

3D structure databases

ProteinModelPortaliP70583.
SMRiP70583. Positions 67-205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1283Substrate bindingBy similarity
Regioni140 – 1434Substrate bindingBy similarity
Regioni199 – 2002Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

eggNOGiCOG0756.
GeneTreeiENSGT00390000018390.
HOGENOMiHOG000028966.
HOVERGENiHBG000175.
InParanoidiP70583.
KOiK01520.
OMAiSENAHIP.
OrthoDBiEOG7J17ZX.
PhylomeDBiP70583.
TreeFamiTF105416.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

P70583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLCALLRS RLQPSLLRSL TLTSAQSLSC GGSRGVRTWS ARTGPGACAD
60 70 80 90 100
PAVSVSKRAR AEDDASLRFV RLSEHATAPT RGSARAAGYD LYSAYDYTIP
110 120 130 140 150
SMEKALVKTD IQIAVPSGCY GRVAPRSGLA VKHFIDVGAG VIDEDYRGNV
160 170 180 190 200
GVVLFNFGKE KFEVKKGDRI AQLICERILY PDLEEVQTLD NTERGSGGFG

STGKN
Length:205
Mass (Da):22,003
Last modified:July 11, 2001 - v3
Checksum:iA9D54EBF5ED015C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U64030 mRNA. Translation: AAC34734.2.
PIRiT10819.
RefSeqiNP_001035361.1. NM_001040271.1.
NP_446044.1. NM_053592.2.
UniGeneiRn.6102.

Genome annotation databases

EnsembliENSRNOT00000009549; ENSRNOP00000009549; ENSRNOG00000007221.
GeneIDi497778.
KEGGirno:497778.
UCSCiRGD:620849. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U64030 mRNA. Translation: AAC34734.2.
PIRiT10819.
RefSeqiNP_001035361.1. NM_001040271.1.
NP_446044.1. NM_053592.2.
UniGeneiRn.6102.

3D structure databases

ProteinModelPortaliP70583.
SMRiP70583. Positions 67-205.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi269088. 4 interactions.
STRINGi10116.ENSRNOP00000009549.

PTM databases

PhosphoSiteiP70583.

Proteomic databases

PaxDbiP70583.
PRIDEiP70583.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009549; ENSRNOP00000009549; ENSRNOG00000007221.
GeneIDi497778.
KEGGirno:497778.
UCSCiRGD:620849. rat.

Organism-specific databases

CTDi1854.
RGDi620849. Dut.

Phylogenomic databases

eggNOGiCOG0756.
GeneTreeiENSGT00390000018390.
HOGENOMiHOG000028966.
HOVERGENiHBG000175.
InParanoidiP70583.
KOiK01520.
OMAiSENAHIP.
OrthoDBiEOG7J17ZX.
PhylomeDBiP70583.
TreeFamiTF105416.

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.
ReactomeiREACT_332139. Pyrimidine biosynthesis.

Miscellaneous databases

NextBioi697760.
PROiP70583.

Gene expression databases

GenevisibleiP70583. RN.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and identification of rat deoxyuridine triphosphatase as an inhibitor of peroxisome proliferator-activated receptor alpha."
    Chu R.Y., Lin Y.L., Rao M.S., Reddy J.K.
    J. Biol. Chem. 271:27670-27676(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. Chu R.Y., Lin Y.L., Rao M.S., Reddy J.K.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiDUT_RAT
AccessioniPrimary (citable) accession number: P70583
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 11, 2001
Last modified: July 22, 2015
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Each trimer binds three substrate molecules. The ligands are bound between subunits, and for each substrate molecule, residues from adjacent subunits contribute to the binding interactions.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.