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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

Dut

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor.

Miscellaneous

Each trimer binds three substrate molecules. The ligands are bound between subunits, and for each substrate molecule, residues from adjacent subunits contribute to the binding interactions.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+By similarity

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (Dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei151Substrate; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

  • dUTP diphosphatase activity Source: RGD
  • magnesium ion binding Source: GO_Central
  • pyrimidine deoxyribonucleotide binding Source: RGD
  • receptor inhibitor activity Source: RGD

GO - Biological processi

  • dUMP biosynthetic process Source: RGD
  • dUTP catabolic process Source: RGD
  • liver development Source: RGD
  • protein homotrimerization Source: RGD
  • regulation of protein heterodimerization activity Source: RGD
  • response to organic cyclic compound Source: RGD

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-499943 Interconversion of nucleotide di- and triphosphates
UniPathwayiUPA00610; UER00666

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
PPAR-interacting protein 4
Short name:
PIP4
dUTP pyrophosphatase
Gene namesi
Name:Dut
Synonyms:Dutp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi620849 Dut

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001829261 – 205Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST205

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP70583
PRIDEiP70583

PTM databases

iPTMnetiP70583
PhosphoSitePlusiP70583

Expressioni

Tissue specificityi

Expressed in all tissues examined. Higher levels in heart and kidney.

Gene expression databases

BgeeiENSRNOG00000007221
GenevisibleiP70583 RN

Interactioni

Protein-protein interaction databases

BioGridi269088, 4 interactors
STRINGi10116.ENSRNOP00000009549

Structurei

3D structure databases

ProteinModelPortaliP70583
SMRiP70583
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 128Substrate bindingBy similarity3
Regioni140 – 143Substrate bindingBy similarity4
Regioni199 – 200Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

eggNOGiKOG3370 Eukaryota
COG0756 LUCA
GeneTreeiENSGT00390000018390
HOGENOMiHOG000028966
HOVERGENiHBG000175
InParanoidiP70583
KOiK01520
OMAiPGFEAQI
OrthoDBiEOG091G14FN
PhylomeDBiP70583
TreeFamiTF105416

Family and domain databases

CDDicd07557 trimeric_dUTPase, 1 hit
Gene3Di2.70.40.10, 1 hit
InterProiView protein in InterPro
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR008181 dUTPase_1
IPR033704 dUTPase_trimeric
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
SUPFAMiSSF51283 SSF51283, 1 hit
TIGRFAMsiTIGR00576 dut, 1 hit

Sequencei

Sequence statusi: Complete.

P70583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLCALLRS RLQPSLLRSL TLTSAQSLSC GGSRGVRTWS ARTGPGACAD
60 70 80 90 100
PAVSVSKRAR AEDDASLRFV RLSEHATAPT RGSARAAGYD LYSAYDYTIP
110 120 130 140 150
SMEKALVKTD IQIAVPSGCY GRVAPRSGLA VKHFIDVGAG VIDEDYRGNV
160 170 180 190 200
GVVLFNFGKE KFEVKKGDRI AQLICERILY PDLEEVQTLD NTERGSGGFG

STGKN
Length:205
Mass (Da):22,003
Last modified:July 11, 2001 - v3
Checksum:iA9D54EBF5ED015C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U64030 mRNA Translation: AAC34734.2
PIRiT10819
RefSeqiNP_001035361.1, NM_001040271.1
NP_446044.1, NM_053592.2
UniGeneiRn.6102

Genome annotation databases

EnsembliENSRNOT00000009549; ENSRNOP00000009549; ENSRNOG00000007221
GeneIDi497778
KEGGirno:497778
UCSCiRGD:620849 rat

Similar proteinsi

Entry informationi

Entry nameiDUT_RAT
AccessioniPrimary (citable) accession number: P70583
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 11, 2001
Last modified: May 23, 2018
This is version 142 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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