Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P70583

- DUT_RAT

UniProt

P70583 - DUT_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

Dut

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Magnesium.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei151 – 1511Substrate; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: RGD
  2. metal ion binding Source: UniProtKB-KW
  3. peroxisome proliferator activated receptor binding Source: RGD
  4. poly(A) RNA binding Source: Ensembl
  5. pyrimidine deoxyribonucleotide binding Source: RGD
  6. receptor inhibitor activity Source: RGD

GO - Biological processi

  1. dUMP biosynthetic process Source: RGD
  2. dUTP catabolic process Source: RGD
  3. liver development Source: RGD
  4. negative regulation of receptor activity Source: GOC
  5. protein homotrimerization Source: RGD
  6. regulation of protein heterodimerization activity Source: RGD
  7. response to organic cyclic compound Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_212687. Pyrimidine biosynthesis.
UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
PPAR-interacting protein 4
Short name:
PIP4
dUTP pyrophosphatase
Gene namesi
Name:Dut
Synonyms:Dutp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi620849. Dut.

Subcellular locationi

Cytoplasm. Nucleus
Note: Binding to PPAR induces translocation to the nucleus.

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. cytosol Source: RGD
  3. extracellular vesicular exosome Source: Ensembl
  4. mitochondrion Source: Ensembl
  5. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000182926Add
BLAST

Proteomic databases

PaxDbiP70583.
PRIDEiP70583.

PTM databases

PhosphoSiteiP70583.

Expressioni

Tissue specificityi

Expressed in all tissues examined. Higher levels in heart and kidney.

Gene expression databases

GenevestigatoriP70583.

Interactioni

Protein-protein interaction databases

BioGridi269088. 4 interactions.
STRINGi10116.ENSRNOP00000009549.

Structurei

3D structure databases

ProteinModelPortaliP70583.
SMRiP70583. Positions 67-205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1283Substrate bindingBy similarity
Regioni140 – 1434Substrate bindingBy similarity
Regioni199 – 2002Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

eggNOGiCOG0756.
GeneTreeiENSGT00390000018390.
HOGENOMiHOG000028966.
HOVERGENiHBG000175.
InParanoidiP70583.
KOiK01520.
OMAiAVKHGIQ.
OrthoDBiEOG7J17ZX.
PhylomeDBiP70583.
TreeFamiTF105416.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

P70583-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLLCALLRS RLQPSLLRSL TLTSAQSLSC GGSRGVRTWS ARTGPGACAD
60 70 80 90 100
PAVSVSKRAR AEDDASLRFV RLSEHATAPT RGSARAAGYD LYSAYDYTIP
110 120 130 140 150
SMEKALVKTD IQIAVPSGCY GRVAPRSGLA VKHFIDVGAG VIDEDYRGNV
160 170 180 190 200
GVVLFNFGKE KFEVKKGDRI AQLICERILY PDLEEVQTLD NTERGSGGFG

STGKN
Length:205
Mass (Da):22,003
Last modified:July 11, 2001 - v3
Checksum:iA9D54EBF5ED015C4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U64030 mRNA. Translation: AAC34734.2.
PIRiT10819.
RefSeqiNP_001035361.1. NM_001040271.1.
NP_446044.1. NM_053592.2.
UniGeneiRn.6102.

Genome annotation databases

EnsembliENSRNOT00000009549; ENSRNOP00000009549; ENSRNOG00000007221.
GeneIDi497778.
KEGGirno:497778.
UCSCiRGD:620849. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U64030 mRNA. Translation: AAC34734.2 .
PIRi T10819.
RefSeqi NP_001035361.1. NM_001040271.1.
NP_446044.1. NM_053592.2.
UniGenei Rn.6102.

3D structure databases

ProteinModelPortali P70583.
SMRi P70583. Positions 67-205.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 269088. 4 interactions.
STRINGi 10116.ENSRNOP00000009549.

PTM databases

PhosphoSitei P70583.

Proteomic databases

PaxDbi P70583.
PRIDEi P70583.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000009549 ; ENSRNOP00000009549 ; ENSRNOG00000007221 .
GeneIDi 497778.
KEGGi rno:497778.
UCSCi RGD:620849. rat.

Organism-specific databases

CTDi 1854.
RGDi 620849. Dut.

Phylogenomic databases

eggNOGi COG0756.
GeneTreei ENSGT00390000018390.
HOGENOMi HOG000028966.
HOVERGENi HBG000175.
InParanoidi P70583.
KOi K01520.
OMAi AVKHGIQ.
OrthoDBi EOG7J17ZX.
PhylomeDBi P70583.
TreeFami TF105416.

Enzyme and pathway databases

UniPathwayi UPA00610 ; UER00666 .
Reactomei REACT_212687. Pyrimidine biosynthesis.

Miscellaneous databases

NextBioi 697760.
PROi P70583.

Gene expression databases

Genevestigatori P70583.

Family and domain databases

Gene3Di 2.70.40.10. 1 hit.
InterProi IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
[Graphical view ]
SUPFAMi SSF51283. SSF51283. 1 hit.
TIGRFAMsi TIGR00576. dut. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and identification of rat deoxyuridine triphosphatase as an inhibitor of peroxisome proliferator-activated receptor alpha."
    Chu R.Y., Lin Y.L., Rao M.S., Reddy J.K.
    J. Biol. Chem. 271:27670-27676(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. Chu R.Y., Lin Y.L., Rao M.S., Reddy J.K.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiDUT_RAT
AccessioniPrimary (citable) accession number: P70583
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 11, 2001
Last modified: October 29, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Each trimer binds three substrate molecules. The ligands are bound between subunits, and for each substrate molecule, residues from adjacent subunits contribute to the binding interactions.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3