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Protein

Nucleoporin p58/p45

Gene

Nup58

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane.1 Publication

GO - Molecular functioni

  • nuclear localization sequence binding Source: RGD
  • nucleocytoplasmic transporter activity Source: UniProtKB

GO - Biological processi

  • mRNA transport Source: UniProtKB-KW
  • nucleocytoplasmic transport Source: UniProtKB
  • protein heterooligomerization Source: RGD
  • protein heterotetramerization Source: RGD
  • protein heterotrimerization Source: RGD
  • protein homooligomerization Source: RGD
  • protein transport Source: UniProtKB-KW
  • regulation of protein import into nucleus Source: RGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-RNO-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-RNO-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-RNO-3108214. SUMOylation of DNA damage response and repair proteins.
R-RNO-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-RNO-3371453. Regulation of HSF1-mediated heat shock response.
R-RNO-4570464. SUMOylation of RNA binding proteins.
R-RNO-4615885. SUMOylation of DNA replication proteins.
R-RNO-5578749. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin p58/p45Curated
Alternative name(s):
58 kDa nucleoporinImported
Nucleoporin-like protein 1
Gene namesi
Name:Nup58Imported
Synonyms:Nupl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi631334. Nup58.

Subcellular locationi

  • Nucleusnuclear pore complex 1 Publication
  • Nucleus membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
  • Nucleus membrane 1 Publication; Peripheral membrane protein 1 Publication; Nucleoplasmic side 1 Publication

  • Note: Biased towards cytoplasmic side. Central region of the nuclear pore complex, within the transporter.

GO - Cellular componenti

  • nuclear envelope Source: RGD
  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: UniProtKB
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585Nucleoporin p58/p45PRO_0000204894Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei317 – 3171PhosphothreonineBy similarity

Post-translational modificationi

O-glycosylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP70581.
PRIDEiP70581.

Expressioni

Tissue specificityi

Expressed in liver.1 Publication

Gene expression databases

BgeeiENSRNOG00000012644.
ExpressionAtlasiP70581. baseline.
GenevisibleiP70581. RN.

Interactioni

Subunit structurei

Component of the p62 complex, a complex composed of NUP62, NUP54, and the isoform p58 and isoform p45 of NUP58. Isoform p58 interacts with NUTF2. Isoform p58 interacts with SRP1-alpha and Importin p97 proteins when they are together, but not with SRP1-alpha protein alone.1 Publication

Protein-protein interaction databases

BioGridi251446. 1 interaction.
STRINGi10116.ENSRNOP00000017204.

Structurei

Secondary structure

1
585
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi328 – 35730Combined sources
Helixi360 – 3634Combined sources
Helixi368 – 41043Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OSZX-ray2.85A/B/C/D327-415[»]
3T98X-ray2.50B327-415[»]
ProteinModelPortaliP70581.
SMRiP70581. Positions 327-411.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70581.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati7 – 821
Repeati30 – 3122
Repeati42 – 4323
Repeati61 – 6224
Repeati66 – 6725
Repeati474 – 47526
Repeati478 – 47927
Repeati499 – 50028
Repeati505 – 50629
Repeati515 – 516210
Repeati517 – 518211
Repeati531 – 532212
Repeati554 – 555213
Repeati564 – 565214

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 56555914 X 2 AA repeats of F-GAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili242 – 26221Sequence analysisAdd
BLAST
Coiled coili300 – 36768Sequence analysisAdd
BLAST

Domaini

Contains FG repeats.

Sequence similaritiesi

Belongs to the NUP58 family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IIR3. Eukaryota.
ENOG410ZJAM. LUCA.
GeneTreeiENSGT00730000111111.
HOGENOMiHOG000273874.
HOVERGENiHBG052703.
InParanoidiP70581.
KOiK14307.
OMAiYRKTFLG.
OrthoDBiEOG091G0KUR.
PhylomeDBiP70581.

Family and domain databases

InterProiIPR024882. Nucleoporin_p58/p45.
[Graphical view]
PANTHERiPTHR13437. PTHR13437. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform p58 (identifier: P70581-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATGFSFGSG TLGSTTVAPG GTGTGSGFSF GASSTPSVGL NFGTLGSSAT
60 70 80 90 100
PASTSTSASG FGTGLFGSKP GTGFTLGGTS AGTTATTSAS TTGFSLGFSK
110 120 130 140 150
PAASATPFAL PVTSTTASGL TLSSALTSAP AASTGFTLNN LGATPATTTA
160 170 180 190 200
ASTGLSLGGA LAGLGGSLFQ SGNTATSGLG QNALSLSLGT ATPTSAASSE
210 220 230 240 250
GLGGIDFSTS SDKKSDKTGT RPEDSKALKD ENLPPVICQD VENLQKFVKE
260 270 280 290 300
QKQVQEEISR MSSKAMLKVQ EDIKALKQLL SLAASGLQRN TLNIDKLKLE
310 320 330 340 350
TAQELKNAEI ALRTQKTPPG LQHENTAPAD YFRVLVQQFE VQLQQYRQQI
360 370 380 390 400
EELENHLATQ ANNSHITPQD LSMAMQKIYQ TFVALAAQLQ SIHENVKVLK
410 420 430 440 450
EQYLSYRKMF LGDAGDVFEA RRTEAKKWQN APRVTTGPTP FSTMPNAAAV
460 470 480 490 500
AMAATLTQQQ QPATGPQPSL GVSFGTPFGS GIGTGLQSSG LGSSNLGGFG
510 520 530 540 550
TSSGFGCGTT GASTFGFGTT DKPSGSLSAG FGSSSTSGFN FSNPGITASA
560 570 580
GLTFGVSNPA SAGFGTGGQL LQLKRPPAGN KRGKR
Length:585
Mass (Da):59,265
Last modified:February 1, 1997 - v1
Checksum:i4D5F5C2744A75C3C
GO
Isoform p45 (identifier: P70581-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     224-245: Missing.
     531-535: FGSSS → LCASA
     536-585: Missing.

Show »
Length:513
Mass (Da):51,821
Checksum:iC70EC7465F5AAD0B
GO
Isoform p23 (identifier: P70581-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-260: Missing.
     328-337: PADYFRVLVQ → SPDDERLQVH
     466-468: PQP → DGW
     469-585: Missing.

Note: No experimental confirmation available.
Show »
Length:208
Mass (Da):23,325
Checksum:i372EE982D097DBCD
GO
Isoform H6 (identifier: P70581-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     466-471: PQPSLG → LNAFKL
     472-585: Missing.

Note: No experimental confirmation available.
Show »
Length:471
Mass (Da):48,579
Checksum:i689BE7709B203C17
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 260260Missing in isoform p23. 1 PublicationVSP_008582Add
BLAST
Alternative sequencei224 – 24522Missing in isoform p45. 1 PublicationVSP_008579Add
BLAST
Alternative sequencei328 – 33710PADYFRVLVQ → SPDDERLQVH in isoform p23. 1 PublicationVSP_008576
Alternative sequencei466 – 4716PQPSLG → LNAFKL in isoform H6. CuratedVSP_008583
Alternative sequencei466 – 4683PQP → DGW in isoform p23. 1 PublicationVSP_008577
Alternative sequencei469 – 585117Missing in isoform p23. 1 PublicationVSP_008578Add
BLAST
Alternative sequencei472 – 585114Missing in isoform H6. CuratedVSP_008584Add
BLAST
Alternative sequencei531 – 5355FGSSS → LCASA in isoform p45. 1 PublicationVSP_008580
Alternative sequencei536 – 58550Missing in isoform p45. 1 PublicationVSP_008581Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63839 mRNA. Translation: AAC52789.1.
AH007036 mRNA. Translation: AAC82539.1.
AF000900 mRNA. Translation: AAC82318.1.
BC085690 mRNA. Translation: AAH85690.1.
AF000901 mRNA. Translation: AAC82319.1.
RefSeqiNP_620791.1. NM_139091.2. [P70581-1]
UniGeneiRn.11099.

Genome annotation databases

EnsembliENSRNOT00000017204; ENSRNOP00000017204; ENSRNOG00000012644. [P70581-1]
GeneIDi245922.
KEGGirno:245922.
UCSCiRGD:631334. rat. [P70581-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63839 mRNA. Translation: AAC52789.1.
AH007036 mRNA. Translation: AAC82539.1.
AF000900 mRNA. Translation: AAC82318.1.
BC085690 mRNA. Translation: AAH85690.1.
AF000901 mRNA. Translation: AAC82319.1.
RefSeqiNP_620791.1. NM_139091.2. [P70581-1]
UniGeneiRn.11099.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OSZX-ray2.85A/B/C/D327-415[»]
3T98X-ray2.50B327-415[»]
ProteinModelPortaliP70581.
SMRiP70581. Positions 327-411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251446. 1 interaction.
STRINGi10116.ENSRNOP00000017204.

Proteomic databases

PaxDbiP70581.
PRIDEiP70581.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017204; ENSRNOP00000017204; ENSRNOG00000012644. [P70581-1]
GeneIDi245922.
KEGGirno:245922.
UCSCiRGD:631334. rat. [P70581-1]

Organism-specific databases

CTDi9818.
RGDi631334. Nup58.

Phylogenomic databases

eggNOGiENOG410IIR3. Eukaryota.
ENOG410ZJAM. LUCA.
GeneTreeiENSGT00730000111111.
HOGENOMiHOG000273874.
HOVERGENiHBG052703.
InParanoidiP70581.
KOiK14307.
OMAiYRKTFLG.
OrthoDBiEOG091G0KUR.
PhylomeDBiP70581.

Enzyme and pathway databases

ReactomeiR-RNO-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-RNO-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-RNO-3108214. SUMOylation of DNA damage response and repair proteins.
R-RNO-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-RNO-3371453. Regulation of HSF1-mediated heat shock response.
R-RNO-4570464. SUMOylation of RNA binding proteins.
R-RNO-4615885. SUMOylation of DNA replication proteins.
R-RNO-5578749. Transcriptional regulation by small RNAs.

Miscellaneous databases

EvolutionaryTraceiP70581.
PROiP70581.

Gene expression databases

BgeeiENSRNOG00000012644.
ExpressionAtlasiP70581. baseline.
GenevisibleiP70581. RN.

Family and domain databases

InterProiIPR024882. Nucleoporin_p58/p45.
[Graphical view]
PANTHERiPTHR13437. PTHR13437. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNUP58_RAT
AccessioniPrimary (citable) accession number: P70581
Secondary accession number(s): Q9JHE1, Q9QWK7, Q9Z2W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.