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P70579

- GRM8_RAT

UniProt

P70579 - GRM8_RAT

Protein

Metabotropic glutamate receptor 8

Gene

Grm8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei156 – 1561GlutamateBy similarity
    Binding sitei227 – 2271GlutamateBy similarity
    Binding sitei309 – 3091GlutamateBy similarity
    Binding sitei401 – 4011GlutamateBy similarity

    GO - Molecular functioni

    1. glutamate receptor activity Source: UniProtKB
    2. G-protein coupled receptor activity Source: UniProtKB
    3. group III metabotropic glutamate receptor activity Source: RGD

    GO - Biological processi

    1. adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
    2. detection of mechanical stimulus involved in sensory perception of pain Source: RGD
    3. regulation of neuron death Source: UniProtKB
    4. regulation of neurotransmitter secretion Source: UniProtKB
    5. regulation of synaptic transmission, glutamatergic Source: RefGenome
    6. sensory perception of pain Source: UniProtKB
    7. sensory perception of smell Source: UniProtKB-KW
    8. synaptic transmission Source: UniProtKB
    9. synaptic transmission, glutamatergic Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Olfaction, Sensory transduction

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metabotropic glutamate receptor 8
    Short name:
    mGluR8
    Gene namesi
    Name:Grm8
    Synonyms:Gprc1h, Mglur8
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi619858. Grm8.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: UniProtKB
    2. neuronal cell body Source: RGD
    3. neuron projection Source: RGD
    4. postsynaptic membrane Source: UniProtKB
    5. presynaptic membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence AnalysisAdd
    BLAST
    Chaini34 – 908875Metabotropic glutamate receptor 8PRO_0000012943Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi64 ↔ 106By similarity
    Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi246 ↔ 534By similarity
    Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi369 ↔ 384By similarity
    Disulfide bondi424 ↔ 431By similarity
    Glycosylationi452 – 4521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi480 – 4801N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi516 ↔ 535By similarity
    Disulfide bondi520 ↔ 538By similarity
    Disulfide bondi541 ↔ 553By similarity
    Disulfide bondi556 ↔ 569By similarity
    Glycosylationi565 – 5651N-linked (GlcNAc...)Sequence Analysis
    Cross-linki882 – 882Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1)By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Isopeptide bond, Ubl conjugation

    Proteomic databases

    PaxDbiP70579.

    PTM databases

    PhosphoSiteiP70579.

    Expressioni

    Tissue specificityi

    Prominent expression in olfactory bulb, pontine gray, lateral reticular nucleus of the thalamus, and piriform cortex. Less abundant expression incerebral cortex, hippocampus, cerebellum, and mammillary body.

    Gene expression databases

    GenevestigatoriP70579.

    Interactioni

    Subunit structurei

    Interacts with PICK1.1 Publication

    Protein-protein interaction databases

    BioGridi248875. 16 interactions.
    IntActiP70579. 5 interactions.
    MINTiMINT-235225.
    STRINGi10116.ENSRNOP00000035953.

    Structurei

    3D structure databases

    ProteinModelPortaliP70579.
    SMRiP70579. Positions 38-504.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini34 – 583550ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini609 – 62012CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini642 – 6476ExtracellularSequence Analysis
    Topological domaini669 – 69527CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini717 – 74630ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini769 – 78113CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini804 – 81815ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini844 – 90865CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei584 – 60825Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei621 – 64121Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei648 – 66821Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei696 – 71621Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei747 – 76822Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei782 – 80322Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei819 – 84325Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni177 – 1793Glutamate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG295200.
    HOGENOMiHOG000218635.
    HOVERGENiHBG107965.
    InParanoidiP70579.
    KOiK04608.
    PhylomeDBiP70579.

    Family and domain databases

    InterProiIPR001828. ANF_lig-bd_rcpt.
    IPR000337. GPCR_3.
    IPR011500. GPCR_3_9-Cys_dom.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR000162. GPCR_3_mtglu_rcpt.
    IPR000144. GPCR_3_mtglu_rcpt_8.
    IPR028082. Peripla_BP_I.
    [Graphical view]
    PfamiPF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    PF07562. NCD3G. 1 hit.
    [Graphical view]
    PRINTSiPR00248. GPCRMGR.
    PR01058. MTABOTROPC8R.
    PR00593. MTABOTROPICR.
    SUPFAMiSSF53822. SSF53822. 1 hit.
    PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
    PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
    PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P70579-1 [UniParc]FASTAAdd to Basket

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    MVCEGKRLAS CPCFFLLTAK FYWILTMMQR THSQEYAHSI RVDGDIILGG    50
    LFPVHAKGER GVPCGELKKE KGIHRLEAML YAIDQINKDP DLLSNITLGV 100
    RILDTCSRDT YALEQSLTFV QALIEKDASD VKCANGDPPI FTKPDKISGV 150
    IGAAASSVSI MVANILRLFK IPQISYASTA PELSDNTRYD FFSRVVPPDS 200
    YQAQAMVDIV TALGWNYVST LASEGNYGES GVEAFTQISR EIGGVCIAQS 250
    QKIPREPRPG EFEKIIKRLL ETPNARAVIM FANEDDIRRI LEAAKKLNQS 300
    GHFLWIGSDS WGSKIAPVYQ QEEIAEGAVT ILPKRASIDG FDRYFRSRTL 350
    ANNRRNVWFA EFWEENFGCK LGSHGKRNSH IKKCTGLERI ARDSSYEQEG 400
    KVQFVIDAVY SMAYALHNMH KERCPGYIGL CPRMVTIDGK ELLGYIRAVN 450
    FNGSAGTPVT FNENGDAPGR YDIFQYQINN KSTEYKIIGH WTNQLHLKVE 500
    DMQWANREHT HPASVCSLPC KPGERKKTVK GVPCCWHCER CEGYNYQVDE 550
    LSCELCPLDQ RPNINRTGCQ RIPIIKLEWH SPWAVVPVFI AILGIIATTF 600
    VIVTFVRYND TPIVRASGRE LSYVLLTGIF LCYSITFLMI AAPDTIICSF 650
    RRIFLGLGMC FSYAALLTKT NRIHRIFEQG KKSVTAPKFI SPASQLVITF 700
    SLISVQLLGV FVWFVVDPPH TIIDYGEQRT LDPENARGVL KCDISDLSLI 750
    CSLGYSILLM VTCTVYAIKT RGVPETFNEA KPIGFTMYTT CIIWLAFIPI 800
    FFGTAQSAEK MYIQTTTLTV SMSLSASVSL GMLYMPKVYI IIFHPEQNVQ 850
    KRKRSFKAVV TAATMQSKLI QKGNDRPNGE VKSELCESLE TNTSSTKTTY 900
    ISYSNHSI 908
    Length:908
    Mass (Da):101,867
    Last modified:February 1, 1997 - v1
    Checksum:iFCAB54CB8E3DD915
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U63288 mRNA. Translation: AAB09537.1.
    RefSeqiNP_071538.1. NM_022202.1.
    UniGeneiRn.44420.

    Genome annotation databases

    GeneIDi60590.
    KEGGirno:60590.
    UCSCiRGD:619858. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U63288 mRNA. Translation: AAB09537.1 .
    RefSeqi NP_071538.1. NM_022202.1.
    UniGenei Rn.44420.

    3D structure databases

    ProteinModelPortali P70579.
    SMRi P70579. Positions 38-504.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248875. 16 interactions.
    IntActi P70579. 5 interactions.
    MINTi MINT-235225.
    STRINGi 10116.ENSRNOP00000035953.

    Chemistry

    BindingDBi P70579.
    ChEMBLi CHEMBL2718.
    GuidetoPHARMACOLOGYi 296.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P70579.

    Proteomic databases

    PaxDbi P70579.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 60590.
    KEGGi rno:60590.
    UCSCi RGD:619858. rat.

    Organism-specific databases

    CTDi 2918.
    RGDi 619858. Grm8.

    Phylogenomic databases

    eggNOGi NOG295200.
    HOGENOMi HOG000218635.
    HOVERGENi HBG107965.
    InParanoidi P70579.
    KOi K04608.
    PhylomeDBi P70579.

    Miscellaneous databases

    NextBioi 612332.
    PROi P70579.

    Gene expression databases

    Genevestigatori P70579.

    Family and domain databases

    InterProi IPR001828. ANF_lig-bd_rcpt.
    IPR000337. GPCR_3.
    IPR011500. GPCR_3_9-Cys_dom.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR000162. GPCR_3_mtglu_rcpt.
    IPR000144. GPCR_3_mtglu_rcpt_8.
    IPR028082. Peripla_BP_I.
    [Graphical view ]
    Pfami PF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    PF07562. NCD3G. 1 hit.
    [Graphical view ]
    PRINTSi PR00248. GPCRMGR.
    PR01058. MTABOTROPC8R.
    PR00593. MTABOTROPICR.
    SUPFAMi SSF53822. SSF53822. 1 hit.
    PROSITEi PS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
    PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
    PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of rat metabotropic glutamate receptor 8 reveals a distinct pharmacological profile."
      Saugstad J.A., Kinzie J.M., Shinohara M.M., Segerson T.P., Westbrook G.L.
      Mol. Pharmacol. 51:119-125(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Interaction of the C-terminal tail region of the metabotropic glutamate receptor 7 with the protein kinase C substrate PICK1."
      El Far O., Airas J., Wischmeyer E., Nehring R.B., Karschin A., Betz H.
      Eur. J. Neurosci. 12:4215-4221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PICK1.

    Entry informationi

    Entry nameiGRM8_RAT
    AccessioniPrimary (citable) accession number: P70579
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3