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P70579

- GRM8_RAT

UniProt

P70579 - GRM8_RAT

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Protein

Metabotropic glutamate receptor 8

Gene
Grm8, Gprc1h, Mglur8
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei156 – 1561Glutamate By similarity
Binding sitei227 – 2271Glutamate By similarity
Binding sitei309 – 3091Glutamate By similarity
Binding sitei401 – 4011Glutamate By similarity

GO - Molecular functioni

  1. glutamate receptor activity Source: UniProtKB
  2. G-protein coupled receptor activity Source: UniProtKB
  3. group III metabotropic glutamate receptor activity Source: RGD

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
  2. detection of mechanical stimulus involved in sensory perception of pain Source: RGD
  3. regulation of neuron death Source: UniProtKB
  4. regulation of neurotransmitter secretion Source: UniProtKB
  5. regulation of synaptic transmission, glutamatergic Source: RefGenome
  6. sensory perception of pain Source: UniProtKB
  7. sensory perception of smell Source: UniProtKB-KW
  8. synaptic transmission Source: UniProtKB
  9. synaptic transmission, glutamatergic Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Olfaction, Sensory transduction

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 8
Short name:
mGluR8
Gene namesi
Name:Grm8
Synonyms:Gprc1h, Mglur8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi619858. Grm8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini34 – 583550Extracellular Reviewed predictionAdd
BLAST
Transmembranei584 – 60825Helical; Name=1; Reviewed predictionAdd
BLAST
Topological domaini609 – 62012Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei621 – 64121Helical; Name=2; Reviewed predictionAdd
BLAST
Topological domaini642 – 6476Extracellular Reviewed prediction
Transmembranei648 – 66821Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini669 – 69527Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei696 – 71621Helical; Name=4; Reviewed predictionAdd
BLAST
Topological domaini717 – 74630Extracellular Reviewed predictionAdd
BLAST
Transmembranei747 – 76822Helical; Name=5; Reviewed predictionAdd
BLAST
Topological domaini769 – 78113Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei782 – 80322Helical; Name=6; Reviewed predictionAdd
BLAST
Topological domaini804 – 81815Extracellular Reviewed predictionAdd
BLAST
Transmembranei819 – 84325Helical; Name=7; Reviewed predictionAdd
BLAST
Topological domaini844 – 90865Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: UniProtKB
  2. neuronal cell body Source: RGD
  3. neuron projection Source: RGD
  4. postsynaptic membrane Source: UniProtKB
  5. presynaptic membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333 Reviewed predictionAdd
BLAST
Chaini34 – 908875Metabotropic glutamate receptor 8PRO_0000012943Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi64 ↔ 106 By similarity
Glycosylationi95 – 951N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi246 ↔ 534 By similarity
Glycosylationi298 – 2981N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi369 ↔ 384 By similarity
Disulfide bondi424 ↔ 431 By similarity
Glycosylationi452 – 4521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi480 – 4801N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi516 ↔ 535 By similarity
Disulfide bondi520 ↔ 538 By similarity
Disulfide bondi541 ↔ 553 By similarity
Disulfide bondi556 ↔ 569 By similarity
Glycosylationi565 – 5651N-linked (GlcNAc...) Reviewed prediction
Cross-linki882 – 882Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP70579.

PTM databases

PhosphoSiteiP70579.

Expressioni

Tissue specificityi

Prominent expression in olfactory bulb, pontine gray, lateral reticular nucleus of the thalamus, and piriform cortex. Less abundant expression incerebral cortex, hippocampus, cerebellum, and mammillary body.

Gene expression databases

GenevestigatoriP70579.

Interactioni

Subunit structurei

Interacts with PICK1.1 Publication

Protein-protein interaction databases

BioGridi248875. 16 interactions.
IntActiP70579. 5 interactions.
MINTiMINT-235225.
STRINGi10116.ENSRNOP00000035953.

Structurei

3D structure databases

ProteinModelPortaliP70579.
SMRiP70579. Positions 38-504.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni177 – 1793Glutamate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295200.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiP70579.
KOiK04608.
PhylomeDBiP70579.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000144. GPCR_3_mtglu_rcpt_8.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01058. MTABOTROPC8R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70579-1 [UniParc]FASTAAdd to Basket

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MVCEGKRLAS CPCFFLLTAK FYWILTMMQR THSQEYAHSI RVDGDIILGG    50
LFPVHAKGER GVPCGELKKE KGIHRLEAML YAIDQINKDP DLLSNITLGV 100
RILDTCSRDT YALEQSLTFV QALIEKDASD VKCANGDPPI FTKPDKISGV 150
IGAAASSVSI MVANILRLFK IPQISYASTA PELSDNTRYD FFSRVVPPDS 200
YQAQAMVDIV TALGWNYVST LASEGNYGES GVEAFTQISR EIGGVCIAQS 250
QKIPREPRPG EFEKIIKRLL ETPNARAVIM FANEDDIRRI LEAAKKLNQS 300
GHFLWIGSDS WGSKIAPVYQ QEEIAEGAVT ILPKRASIDG FDRYFRSRTL 350
ANNRRNVWFA EFWEENFGCK LGSHGKRNSH IKKCTGLERI ARDSSYEQEG 400
KVQFVIDAVY SMAYALHNMH KERCPGYIGL CPRMVTIDGK ELLGYIRAVN 450
FNGSAGTPVT FNENGDAPGR YDIFQYQINN KSTEYKIIGH WTNQLHLKVE 500
DMQWANREHT HPASVCSLPC KPGERKKTVK GVPCCWHCER CEGYNYQVDE 550
LSCELCPLDQ RPNINRTGCQ RIPIIKLEWH SPWAVVPVFI AILGIIATTF 600
VIVTFVRYND TPIVRASGRE LSYVLLTGIF LCYSITFLMI AAPDTIICSF 650
RRIFLGLGMC FSYAALLTKT NRIHRIFEQG KKSVTAPKFI SPASQLVITF 700
SLISVQLLGV FVWFVVDPPH TIIDYGEQRT LDPENARGVL KCDISDLSLI 750
CSLGYSILLM VTCTVYAIKT RGVPETFNEA KPIGFTMYTT CIIWLAFIPI 800
FFGTAQSAEK MYIQTTTLTV SMSLSASVSL GMLYMPKVYI IIFHPEQNVQ 850
KRKRSFKAVV TAATMQSKLI QKGNDRPNGE VKSELCESLE TNTSSTKTTY 900
ISYSNHSI 908
Length:908
Mass (Da):101,867
Last modified:February 1, 1997 - v1
Checksum:iFCAB54CB8E3DD915
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U63288 mRNA. Translation: AAB09537.1.
RefSeqiNP_071538.1. NM_022202.1.
UniGeneiRn.44420.

Genome annotation databases

GeneIDi60590.
KEGGirno:60590.
UCSCiRGD:619858. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U63288 mRNA. Translation: AAB09537.1 .
RefSeqi NP_071538.1. NM_022202.1.
UniGenei Rn.44420.

3D structure databases

ProteinModelPortali P70579.
SMRi P70579. Positions 38-504.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248875. 16 interactions.
IntActi P70579. 5 interactions.
MINTi MINT-235225.
STRINGi 10116.ENSRNOP00000035953.

Chemistry

BindingDBi P70579.
ChEMBLi CHEMBL2718.
GuidetoPHARMACOLOGYi 296.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P70579.

Proteomic databases

PaxDbi P70579.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 60590.
KEGGi rno:60590.
UCSCi RGD:619858. rat.

Organism-specific databases

CTDi 2918.
RGDi 619858. Grm8.

Phylogenomic databases

eggNOGi NOG295200.
HOGENOMi HOG000218635.
HOVERGENi HBG107965.
InParanoidi P70579.
KOi K04608.
PhylomeDBi P70579.

Miscellaneous databases

NextBioi 612332.
PROi P70579.

Gene expression databases

Genevestigatori P70579.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000144. GPCR_3_mtglu_rcpt_8.
IPR028082. Peripla_BP_I.
[Graphical view ]
Pfami PF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view ]
PRINTSi PR00248. GPCRMGR.
PR01058. MTABOTROPC8R.
PR00593. MTABOTROPICR.
SUPFAMi SSF53822. SSF53822. 1 hit.
PROSITEi PS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of rat metabotropic glutamate receptor 8 reveals a distinct pharmacological profile."
    Saugstad J.A., Kinzie J.M., Shinohara M.M., Segerson T.P., Westbrook G.L.
    Mol. Pharmacol. 51:119-125(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Interaction of the C-terminal tail region of the metabotropic glutamate receptor 7 with the protein kinase C substrate PICK1."
    El Far O., Airas J., Wischmeyer E., Nehring R.B., Karschin A., Betz H.
    Eur. J. Neurosci. 12:4215-4221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PICK1.

Entry informationi

Entry nameiGRM8_RAT
AccessioniPrimary (citable) accession number: P70579
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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