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P70579 (GRM8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metabotropic glutamate receptor 8

Short name=mGluR8
Gene names
Name:Grm8
Synonyms:Gprc1h, Mglur8
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length908 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Subunit structure

Interacts with PICK1. Ref.2

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Prominent expression in olfactory bulb, pontine gray, lateral reticular nucleus of the thalamus, and piriform cortex. Less abundant expression incerebral cortex, hippocampus, cerebellum, and mammillary body.

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
   Biological processOlfaction
Sensory transduction
   Cellular componentCell membrane
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Isopeptide bond
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

detection of mechanical stimulus involved in sensory perception of pain

Inferred from direct assay PubMed 18533199. Source: RGD

regulation of neuron death

Traceable author statement PubMed 11561058. Source: UniProtKB

regulation of neurotransmitter secretion

Inferred from direct assay PubMed 16084932. Source: UniProtKB

sensory perception of pain

Traceable author statement PubMed 11561058. Source: UniProtKB

sensory perception of smell

Inferred from electronic annotation. Source: UniProtKB-KW

synaptic transmission

Inferred from direct assay PubMed 15275822. Source: UniProtKB

synaptic transmission, glutamatergic

Inferred from direct assay PubMed 15275822. Source: UniProtKB

   Cellular_componentintegral component of plasma membrane

Inferred from direct assay PubMed 16045496. Source: UniProtKB

neuron projection

Inferred from direct assay PubMed 12443992. Source: RGD

neuronal cell body

Inferred from direct assay PubMed 12443992. Source: RGD

postsynaptic membrane

Traceable author statement PubMed 16084932. Source: UniProtKB

presynaptic membrane

Inferred from direct assay PubMed 15275822. Source: UniProtKB

   Molecular_functionG-protein coupled receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

glutamate receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

group III metabotropic glutamate receptor activity

Inferred from direct assay PubMed 9875342. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Potential
Chain34 – 908875Metabotropic glutamate receptor 8
PRO_0000012943

Regions

Topological domain34 – 583550Extracellular Potential
Transmembrane584 – 60825Helical; Name=1; Potential
Topological domain609 – 62012Cytoplasmic Potential
Transmembrane621 – 64121Helical; Name=2; Potential
Topological domain642 – 6476Extracellular Potential
Transmembrane648 – 66821Helical; Name=3; Potential
Topological domain669 – 69527Cytoplasmic Potential
Transmembrane696 – 71621Helical; Name=4; Potential
Topological domain717 – 74630Extracellular Potential
Transmembrane747 – 76822Helical; Name=5; Potential
Topological domain769 – 78113Cytoplasmic Potential
Transmembrane782 – 80322Helical; Name=6; Potential
Topological domain804 – 81815Extracellular Potential
Transmembrane819 – 84325Helical; Name=7; Potential
Topological domain844 – 90865Cytoplasmic Potential
Region177 – 1793Glutamate binding By similarity

Sites

Binding site1561Glutamate By similarity
Binding site2271Glutamate By similarity
Binding site3091Glutamate By similarity
Binding site4011Glutamate By similarity

Amino acid modifications

Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation2981N-linked (GlcNAc...) Potential
Glycosylation4521N-linked (GlcNAc...) Potential
Glycosylation4801N-linked (GlcNAc...) Potential
Glycosylation5651N-linked (GlcNAc...) Potential
Disulfide bond64 ↔ 106 By similarity
Disulfide bond246 ↔ 534 By similarity
Disulfide bond369 ↔ 384 By similarity
Disulfide bond424 ↔ 431 By similarity
Disulfide bond516 ↔ 535 By similarity
Disulfide bond520 ↔ 538 By similarity
Disulfide bond541 ↔ 553 By similarity
Disulfide bond556 ↔ 569 By similarity
Cross-link882Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) By similarity

Sequences

Sequence LengthMass (Da)Tools
P70579 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: FCAB54CB8E3DD915

FASTA908101,867
        10         20         30         40         50         60 
MVCEGKRLAS CPCFFLLTAK FYWILTMMQR THSQEYAHSI RVDGDIILGG LFPVHAKGER 

        70         80         90        100        110        120 
GVPCGELKKE KGIHRLEAML YAIDQINKDP DLLSNITLGV RILDTCSRDT YALEQSLTFV 

       130        140        150        160        170        180 
QALIEKDASD VKCANGDPPI FTKPDKISGV IGAAASSVSI MVANILRLFK IPQISYASTA 

       190        200        210        220        230        240 
PELSDNTRYD FFSRVVPPDS YQAQAMVDIV TALGWNYVST LASEGNYGES GVEAFTQISR 

       250        260        270        280        290        300 
EIGGVCIAQS QKIPREPRPG EFEKIIKRLL ETPNARAVIM FANEDDIRRI LEAAKKLNQS 

       310        320        330        340        350        360 
GHFLWIGSDS WGSKIAPVYQ QEEIAEGAVT ILPKRASIDG FDRYFRSRTL ANNRRNVWFA 

       370        380        390        400        410        420 
EFWEENFGCK LGSHGKRNSH IKKCTGLERI ARDSSYEQEG KVQFVIDAVY SMAYALHNMH 

       430        440        450        460        470        480 
KERCPGYIGL CPRMVTIDGK ELLGYIRAVN FNGSAGTPVT FNENGDAPGR YDIFQYQINN 

       490        500        510        520        530        540 
KSTEYKIIGH WTNQLHLKVE DMQWANREHT HPASVCSLPC KPGERKKTVK GVPCCWHCER 

       550        560        570        580        590        600 
CEGYNYQVDE LSCELCPLDQ RPNINRTGCQ RIPIIKLEWH SPWAVVPVFI AILGIIATTF 

       610        620        630        640        650        660 
VIVTFVRYND TPIVRASGRE LSYVLLTGIF LCYSITFLMI AAPDTIICSF RRIFLGLGMC 

       670        680        690        700        710        720 
FSYAALLTKT NRIHRIFEQG KKSVTAPKFI SPASQLVITF SLISVQLLGV FVWFVVDPPH 

       730        740        750        760        770        780 
TIIDYGEQRT LDPENARGVL KCDISDLSLI CSLGYSILLM VTCTVYAIKT RGVPETFNEA 

       790        800        810        820        830        840 
KPIGFTMYTT CIIWLAFIPI FFGTAQSAEK MYIQTTTLTV SMSLSASVSL GMLYMPKVYI 

       850        860        870        880        890        900 
IIFHPEQNVQ KRKRSFKAVV TAATMQSKLI QKGNDRPNGE VKSELCESLE TNTSSTKTTY 


ISYSNHSI 

« Hide

References

[1]"Cloning and expression of rat metabotropic glutamate receptor 8 reveals a distinct pharmacological profile."
Saugstad J.A., Kinzie J.M., Shinohara M.M., Segerson T.P., Westbrook G.L.
Mol. Pharmacol. 51:119-125(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Interaction of the C-terminal tail region of the metabotropic glutamate receptor 7 with the protein kinase C substrate PICK1."
El Far O., Airas J., Wischmeyer E., Nehring R.B., Karschin A., Betz H.
Eur. J. Neurosci. 12:4215-4221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PICK1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U63288 mRNA. Translation: AAB09537.1.
RefSeqNP_071538.1. NM_022202.1.
UniGeneRn.44420.

3D structure databases

ProteinModelPortalP70579.
SMRP70579. Positions 38-504.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248875. 16 interactions.
IntActP70579. 5 interactions.
MINTMINT-235225.
STRING10116.ENSRNOP00000035953.

Chemistry

BindingDBP70579.
ChEMBLCHEMBL2111335.
GuidetoPHARMACOLOGY296.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP70579.

Proteomic databases

PaxDbP70579.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID60590.
KEGGrno:60590.
UCSCRGD:619858. rat.

Organism-specific databases

CTD2918.
RGD619858. Grm8.

Phylogenomic databases

eggNOGNOG295200.
HOGENOMHOG000218635.
HOVERGENHBG107965.
InParanoidP70579.
KOK04608.
PhylomeDBP70579.

Gene expression databases

GenevestigatorP70579.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000144. GPCR_3_mtglu_rcpt_8.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
PR01058. MTABOTROPC8R.
PR00593. MTABOTROPICR.
SUPFAMSSF53822. SSF53822. 1 hit.
PROSITEPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio612332.
PROP70579.

Entry information

Entry nameGRM8_RAT
AccessionPrimary (citable) accession number: P70579
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries