P70579 (GRM8_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metabotropic glutamate receptor 8
Short name=mGluR8

Gene names

Name:	Grm8
Synonyms:	Gprc1h, Mglur8

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	908 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.
Subunit structure	Interacts with PICK1. Ref.2
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Prominent expression in olfactory bulb, pontine gray, lateral reticular nucleus of the thalamus, and piriform cortex. Less abundant expression incerebral cortex, hippocampus, cerebellum, and mammillary body.
Sequence similarities	Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB detection of mechanical stimulus involved in sensory perception of pain Inferred from direct assay PubMed 18533199. Source: RGD regulation of neuron death Traceable author statement PubMed 11561058. Source: UniProtKB regulation of neurotransmitter secretion Inferred from direct assay PubMed 16084932. Source: UniProtKB regulation of synaptic transmission, glutamatergic Inferred from Biological aspect of Ancestor. Source: RefGenome sensory perception of pain Traceable author statement PubMed 11561058. Source: UniProtKB sensory perception of smell Inferred from electronic annotation. Source: UniProtKB-KW synaptic transmission Inferred from direct assay PubMed 15275822. Source: UniProtKB synaptic transmission, glutamatergic Inferred from direct assay PubMed 15275822. Source: UniProtKB
Cellular_component	integral component of plasma membrane Inferred from direct assay PubMed 16045496. Source: UniProtKB neuron projection Inferred from direct assay PubMed 12443992. Source: RGD neuronal cell body Inferred from direct assay PubMed 12443992. Source: RGD postsynaptic membrane Traceable author statement PubMed 16084932. Source: UniProtKB presynaptic membrane Inferred from direct assay PubMed 15275822. Source: UniProtKB
Molecular_function	G-protein coupled receptor activity Inferred from sequence or structural similarity. Source: UniProtKB glutamate receptor activity Inferred from sequence or structural similarity. Source: UniProtKB group III metabotropic glutamate receptor activity Inferred from direct assay PubMed 9875342. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 908	875	Metabotropic glutamate receptor 8		PRO_0000012943
Regions
	Topological domain	34 – 583	550	Extracellular Potential
	Transmembrane	584 – 608	25	Helical; Name=1; Potential
	Topological domain	609 – 620	12	Cytoplasmic Potential
	Transmembrane	621 – 641	21	Helical; Name=2; Potential
	Topological domain	642 – 647	6	Extracellular Potential
	Transmembrane	648 – 668	21	Helical; Name=3; Potential
	Topological domain	669 – 695	27	Cytoplasmic Potential
	Transmembrane	696 – 716	21	Helical; Name=4; Potential
	Topological domain	717 – 746	30	Extracellular Potential
	Transmembrane	747 – 768	22	Helical; Name=5; Potential
	Topological domain	769 – 781	13	Cytoplasmic Potential
	Transmembrane	782 – 803	22	Helical; Name=6; Potential
	Topological domain	804 – 818	15	Extracellular Potential
	Transmembrane	819 – 843	25	Helical; Name=7; Potential
	Topological domain	844 – 908	65	Cytoplasmic Potential
	Region	177 – 179	3	Glutamate binding By similarity
Sites
	Binding site	156	1	Glutamate By similarity
	Binding site	227	1	Glutamate By similarity
	Binding site	309	1	Glutamate By similarity
	Binding site	401	1	Glutamate By similarity
Amino acid modifications
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	452	1	N-linked (GlcNAc...) Potential
	Glycosylation	480	1	N-linked (GlcNAc...) Potential
	Glycosylation	565	1	N-linked (GlcNAc...) Potential
	Disulfide bond	64 ↔ 106		By similarity
	Disulfide bond	246 ↔ 534		By similarity
	Disulfide bond	369 ↔ 384		By similarity
	Disulfide bond	424 ↔ 431		By similarity
	Disulfide bond	516 ↔ 535		By similarity
	Disulfide bond	520 ↔ 538		By similarity
	Disulfide bond	541 ↔ 553		By similarity
	Disulfide bond	556 ↔ 569		By similarity
	Cross-link	882		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70579 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: FCAB54CB8E3DD915	FASTA	908	101,867
	10 20 30 40 50 60 MVCEGKRLAS CPCFFLLTAK FYWILTMMQR THSQEYAHSI RVDGDIILGG LFPVHAKGER 70 80 90 100 110 120 GVPCGELKKE KGIHRLEAML YAIDQINKDP DLLSNITLGV RILDTCSRDT YALEQSLTFV 130 140 150 160 170 180 QALIEKDASD VKCANGDPPI FTKPDKISGV IGAAASSVSI MVANILRLFK IPQISYASTA 190 200 210 220 230 240 PELSDNTRYD FFSRVVPPDS YQAQAMVDIV TALGWNYVST LASEGNYGES GVEAFTQISR 250 260 270 280 290 300 EIGGVCIAQS QKIPREPRPG EFEKIIKRLL ETPNARAVIM FANEDDIRRI LEAAKKLNQS 310 320 330 340 350 360 GHFLWIGSDS WGSKIAPVYQ QEEIAEGAVT ILPKRASIDG FDRYFRSRTL ANNRRNVWFA 370 380 390 400 410 420 EFWEENFGCK LGSHGKRNSH IKKCTGLERI ARDSSYEQEG KVQFVIDAVY SMAYALHNMH 430 440 450 460 470 480 KERCPGYIGL CPRMVTIDGK ELLGYIRAVN FNGSAGTPVT FNENGDAPGR YDIFQYQINN 490 500 510 520 530 540 KSTEYKIIGH WTNQLHLKVE DMQWANREHT HPASVCSLPC KPGERKKTVK GVPCCWHCER 550 560 570 580 590 600 CEGYNYQVDE LSCELCPLDQ RPNINRTGCQ RIPIIKLEWH SPWAVVPVFI AILGIIATTF 610 620 630 640 650 660 VIVTFVRYND TPIVRASGRE LSYVLLTGIF LCYSITFLMI AAPDTIICSF RRIFLGLGMC 670 680 690 700 710 720 FSYAALLTKT NRIHRIFEQG KKSVTAPKFI SPASQLVITF SLISVQLLGV FVWFVVDPPH 730 740 750 760 770 780 TIIDYGEQRT LDPENARGVL KCDISDLSLI CSLGYSILLM VTCTVYAIKT RGVPETFNEA 790 800 810 820 830 840 KPIGFTMYTT CIIWLAFIPI FFGTAQSAEK MYIQTTTLTV SMSLSASVSL GMLYMPKVYI 850 860 870 880 890 900 IIFHPEQNVQ KRKRSFKAVV TAATMQSKLI QKGNDRPNGE VKSELCESLE TNTSSTKTTY ISYSNHSI

References

[1]	"Cloning and expression of rat metabotropic glutamate receptor 8 reveals a distinct pharmacological profile." Saugstad J.A., Kinzie J.M., Shinohara M.M., Segerson T.P., Westbrook G.L. Mol. Pharmacol. 51:119-125(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Interaction of the C-terminal tail region of the metabotropic glutamate receptor 7 with the protein kinase C substrate PICK1." El Far O., Airas J., Wischmeyer E., Nehring R.B., Karschin A., Betz H. Eur. J. Neurosci. 12:4215-4221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PICK1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U63288 mRNA. Translation: AAB09537.1.
RefSeq	NP_071538.1. NM_022202.1.
UniGene	Rn.44420.
3D structure databases
ProteinModelPortal	P70579.
SMR	P70579. Positions 38-504.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	248875. 16 interactions.
IntAct	P70579. 5 interactions.
MINT	MINT-235225.
STRING	10116.ENSRNOP00000035953.
Chemistry
BindingDB	P70579.
ChEMBL	CHEMBL2718.
GuidetoPHARMACOLOGY	296.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P70579.
Proteomic databases
PaxDb	P70579.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	60590.
KEGG	rno:60590.
UCSC	RGD:619858. rat.
Organism-specific databases
CTD	2918.
RGD	619858. Grm8.
Phylogenomic databases
eggNOG	NOG295200.
HOGENOM	HOG000218635.
HOVERGEN	HBG107965.
InParanoid	P70579.
KO	K04608.
PhylomeDB	P70579.
Gene expression databases
Genevestigator	P70579.
Family and domain databases
InterPro	IPR001828. ANF_lig-bd_rcpt. IPR000337. GPCR_3. IPR011500. GPCR_3_9-Cys_dom. IPR017978. GPCR_3_C. IPR017979. GPCR_3_CS. IPR000162. GPCR_3_mtglu_rcpt. IPR000144. GPCR_3_mtglu_rcpt_8. IPR028082. Peripla_BP_I. [Graphical view]
Pfam	PF00003. 7tm_3. 1 hit. PF01094. ANF_receptor. 1 hit. PF07562. NCD3G. 1 hit. [Graphical view]
PRINTS	PR00248. GPCRMGR. PR01058. MTABOTROPC8R. PR00593. MTABOTROPICR.
SUPFAM	SSF53822. SSF53822. 1 hit.
PROSITE	PS00979. G_PROTEIN_RECEP_F3_1. 1 hit. PS00980. G_PROTEIN_RECEP_F3_2. 1 hit. PS00981. G_PROTEIN_RECEP_F3_3. 1 hit. PS50259. G_PROTEIN_RECEP_F3_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	612332.
PRO	P70579.

Entry information

Entry name

GRM8_RAT

Accession

Primary (citable) accession number: P70579

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program