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P70579

- GRM8_RAT

UniProt

P70579 - GRM8_RAT

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Protein

Metabotropic glutamate receptor 8

Gene

Grm8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Display
All None

  • Function
  • Names & Taxonomy
  • Subcellular locationSubcell. location
  • Pathology & BiotechPathol./Biotech
  • PTM / Processing
  • Expression
  • Interaction
  • Structure
  • Family & Domains
  • Sequence
  • Cross-references
  • Publications
  • Entry information
  • Miscellaneous
  • Similar proteins
Top

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei156 – 1561GlutamateBy similarity
Binding sitei227 – 2271GlutamateBy similarity
Binding sitei309 – 3091GlutamateBy similarity
Binding sitei401 – 4011GlutamateBy similarity

GO - Molecular functioni

  1. glutamate receptor activity Source: UniProtKB
  2. G-protein coupled receptor activity Source: UniProtKB
  3. group III metabotropic glutamate receptor activity Source: RGD

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
  2. detection of mechanical stimulus involved in sensory perception of pain Source: RGD
  3. regulation of neuron death Source: UniProtKB
  4. regulation of neurotransmitter secretion Source: UniProtKB
  5. regulation of synaptic transmission, glutamatergic Source: RefGenome
  6. sensory perception of pain Source: UniProtKB
  7. sensory perception of smell Source: UniProtKB-KW
  8. synaptic transmission Source: UniProtKB
  9. synaptic transmission, glutamatergic Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Olfaction, Sensory transduction

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 8
Short name:
mGluR8
Gene namesi
Name:Grm8
Synonyms:Gprc1h, Mglur8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi619858. Grm8.

Subcellular locationi

Cell membrane; Multi-pass membrane protein

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini34 – 583550ExtracellularSequence AnalysisAdd
BLAST
Transmembranei584 – 60825Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini609 – 62012CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei621 – 64121Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini642 – 6476ExtracellularSequence Analysis
Transmembranei648 – 66821Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini669 – 69527CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei696 – 71621Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini717 – 74630ExtracellularSequence AnalysisAdd
BLAST
Transmembranei747 – 76822Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini769 – 78113CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei782 – 80322Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini804 – 81815ExtracellularSequence AnalysisAdd
BLAST
Transmembranei819 – 84325Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini844 – 90865CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: UniProtKB
  2. neuronal cell body Source: RGD
  3. neuron projection Source: RGD
  4. postsynaptic membrane Source: UniProtKB
  5. presynaptic membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence AnalysisAdd
BLAST
Chaini34 – 908875Metabotropic glutamate receptor 8PRO_0000012943Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi64 ↔ 106By similarity
Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi246 ↔ 534By similarity
Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi369 ↔ 384By similarity
Disulfide bondi424 ↔ 431By similarity
Glycosylationi452 – 4521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi480 – 4801N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi516 ↔ 535By similarity
Disulfide bondi520 ↔ 538By similarity
Disulfide bondi541 ↔ 553By similarity
Disulfide bondi556 ↔ 569By similarity
Glycosylationi565 – 5651N-linked (GlcNAc...)Sequence Analysis
Cross-linki882 – 882Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP70579.

PTM databases

PhosphoSiteiP70579.

Expressioni

Tissue specificityi

Prominent expression in olfactory bulb, pontine gray, lateral reticular nucleus of the thalamus, and piriform cortex. Less abundant expression incerebral cortex, hippocampus, cerebellum, and mammillary body.

Gene expression databases

GenevestigatoriP70579.

Interactioni

Subunit structurei

Interacts with PICK1.1 Publication

Protein-protein interaction databases

BioGridi248875. 16 interactions.
IntActiP70579. 5 interactions.
MINTiMINT-235225.
STRINGi10116.ENSRNOP00000035953.

Structurei

3D structure databases

ProteinModelPortaliP70579.
SMRiP70579. Positions 38-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni177 – 1793Glutamate bindingBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 3 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295200.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiP70579.
KOiK04608.
PhylomeDBiP70579.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000144. GPCR_3_mtglu_rcpt_8.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01058. MTABOTROPC8R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70579-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVCEGKRLAS CPCFFLLTAK FYWILTMMQR THSQEYAHSI RVDGDIILGG 
        60         70         80         90        100
LFPVHAKGER GVPCGELKKE KGIHRLEAML YAIDQINKDP DLLSNITLGV 
       110        120        130        140        150
RILDTCSRDT YALEQSLTFV QALIEKDASD VKCANGDPPI FTKPDKISGV 
       160        170        180        190        200
IGAAASSVSI MVANILRLFK IPQISYASTA PELSDNTRYD FFSRVVPPDS 
       210        220        230        240        250
YQAQAMVDIV TALGWNYVST LASEGNYGES GVEAFTQISR EIGGVCIAQS 
       260        270        280        290        300
QKIPREPRPG EFEKIIKRLL ETPNARAVIM FANEDDIRRI LEAAKKLNQS 
       310        320        330        340        350
GHFLWIGSDS WGSKIAPVYQ QEEIAEGAVT ILPKRASIDG FDRYFRSRTL 
       360        370        380        390        400
ANNRRNVWFA EFWEENFGCK LGSHGKRNSH IKKCTGLERI ARDSSYEQEG 
       410        420        430        440        450
KVQFVIDAVY SMAYALHNMH KERCPGYIGL CPRMVTIDGK ELLGYIRAVN 
       460        470        480        490        500
FNGSAGTPVT FNENGDAPGR YDIFQYQINN KSTEYKIIGH WTNQLHLKVE 
       510        520        530        540        550
DMQWANREHT HPASVCSLPC KPGERKKTVK GVPCCWHCER CEGYNYQVDE 
       560        570        580        590        600
LSCELCPLDQ RPNINRTGCQ RIPIIKLEWH SPWAVVPVFI AILGIIATTF 
       610        620        630        640        650
VIVTFVRYND TPIVRASGRE LSYVLLTGIF LCYSITFLMI AAPDTIICSF 
       660        670        680        690        700
RRIFLGLGMC FSYAALLTKT NRIHRIFEQG KKSVTAPKFI SPASQLVITF 
       710        720        730        740        750
SLISVQLLGV FVWFVVDPPH TIIDYGEQRT LDPENARGVL KCDISDLSLI 
       760        770        780        790        800
CSLGYSILLM VTCTVYAIKT RGVPETFNEA KPIGFTMYTT CIIWLAFIPI 
       810        820        830        840        850
FFGTAQSAEK MYIQTTTLTV SMSLSASVSL GMLYMPKVYI IIFHPEQNVQ 
       860        870        880        890        900
KRKRSFKAVV TAATMQSKLI QKGNDRPNGE VKSELCESLE TNTSSTKTTY 

ISYSNHSI
Length:908
Mass (Da):101,867
Last modified:February 1, 1997 - v1
Checksum:iFCAB54CB8E3DD915
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63288 mRNA. Translation: AAB09537.1.
RefSeqiNP_071538.1. NM_022202.1.
UniGeneiRn.44420.

Genome annotation databases

GeneIDi60590.
KEGGirno:60590.
UCSCiRGD:619858. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
 U63288 mRNA. Translation: AAB09537.1 .
RefSeqi NP_071538.1. NM_022202.1.
UniGenei Rn.44420.

3D structure databases

ProteinModelPortali P70579.
SMRi P70579. Positions 38-504.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248875. 16 interactions.
IntActi P70579. 5 interactions.
MINTi MINT-235225.
STRINGi 10116.ENSRNOP00000035953.

Chemistry

BindingDBi P70579.
ChEMBLi CHEMBL2111335.
GuidetoPHARMACOLOGYi 296.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P70579.

Proteomic databases

PaxDbi P70579.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 60590.
KEGGi rno:60590.
UCSCi RGD:619858. rat.

Organism-specific databases

CTDi 2918.
RGDi 619858. Grm8.

Phylogenomic databases

eggNOGi NOG295200.
HOGENOMi HOG000218635.
HOVERGENi HBG107965.
InParanoidi P70579.
KOi K04608.
PhylomeDBi P70579.

Miscellaneous databases

NextBioi 612332.
PROi P70579.

Gene expression databases

Genevestigatori P70579.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000144. GPCR_3_mtglu_rcpt_8.
IPR028082. Peripla_BP_I.
[Graphical view ]
Pfami PF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view ]
PRINTSi PR00248. GPCRMGR.
PR01058. MTABOTROPC8R.
PR00593. MTABOTROPICR.
SUPFAMi SSF53822. SSF53822. 1 hit.
PROSITEi PS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of rat metabotropic glutamate receptor 8 reveals a distinct pharmacological profile."
    Saugstad J.A., Kinzie J.M., Shinohara M.M., Segerson T.P., Westbrook G.L.
    Mol. Pharmacol. 51:119-125(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Interaction of the C-terminal tail region of the metabotropic glutamate receptor 7 with the protein kinase C substrate PICK1."
    El Far O., Airas J., Wischmeyer E., Nehring R.B., Karschin A., Betz H.
    Eur. J. Neurosci. 12:4215-4221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PICK1.
+Additional computationally mapped references.

Entry informationi

Entry nameiGRM8_RAT
AccessioniPrimary (citable) accession number: P70579
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.
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