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Protein

Unconventional myosin-Vb

Gene

Myo5b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi163 – 1708ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ionotropic glutamate receptor binding Source: RGD
  • motor activity Source: InterPro
  • Rab GTPase binding Source: RGD

GO - Biological processi

  • endosome localization Source: UniProtKB
  • protein transport Source: RGD
  • regulation of protein localization Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-Vb
Alternative name(s):
Myosin heavy chain myr 6
Gene namesi
Name:Myo5b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621347. Myo5b.

Subcellular locationi

GO - Cellular componenti

  • apical cortex Source: UniProtKB
  • dendritic spine Source: RGD
  • myosin complex Source: UniProtKB-KW
  • neuronal cell body Source: RGD
  • neuron projection Source: RGD
  • perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18461846Unconventional myosin-VbPRO_0000123462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1444 – 14441PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP70569.
PRIDEiP70569.

PTM databases

iPTMnetiP70569.
PhosphoSiteiP70569.

Interactioni

Subunit structurei

Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with RAB11FIP2 (By similarity). Interacts with RAB11A and RAB8A (By similarity). Found in a complex with CFTR and RAB11A (By similarity). Interacts with NPC1L1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Gria1P194902EBI-975940,EBI-371642

GO - Molecular functioni

  • ionotropic glutamate receptor binding Source: RGD
  • Rab GTPase binding Source: RGD

Protein-protein interaction databases

BioGridi247201. 2 interactions.
IntActiP70569. 8 interactions.
STRINGi10116.ENSRNOP00000019512.

Structurei

3D structure databases

ProteinModelPortaliP70569.
SMRiP70569. Positions 4-790.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 763695Myosin motorAdd
BLAST
Domaini767 – 78822IQ 1PROSITE-ProRule annotationAdd
BLAST
Domaini789 – 81325IQ 2PROSITE-ProRule annotationAdd
BLAST
Domaini814 – 83724IQ 3PROSITE-ProRule annotationAdd
BLAST
Domaini838 – 86124IQ 4PROSITE-ProRule annotationAdd
BLAST
Domaini862 – 88423IQ 5PROSITE-ProRule annotationAdd
BLAST
Domaini885 – 91430IQ 6PROSITE-ProRule annotationAdd
BLAST
Domaini1524 – 1801278DilutePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni641 – 66323Actin-bindingSequence analysisAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili915 – 1272358Sequence analysisAdd
BLAST
Coiled coili1334 – 1450117Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 dilute domain.PROSITE-ProRule annotation
Contains 6 IQ domains.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0160. Eukaryota.
KOG0161. Eukaryota.
COG5022. LUCA.
HOGENOMiHOG000171839.
HOVERGENiHBG052556.
InParanoidiP70569.
KOiK10357.
PhylomeDBiP70569.

Family and domain databases

InterProiIPR002710. Dilute_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01843. DIL. 1 hit.
PF00612. IQ. 6 hits.
PF00063. Myosin_head. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM01132. DIL. 1 hit.
SM00015. IQ. 6 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51126. DILUTE. 1 hit.
PS50096. IQ. 6 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70569-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYSELYSRY TRVWIPDPDE VWRSAELTKD YKDGDESLQL RLEDDTILDY
60 70 80 90 100
PIDVQNNQVP FLRNPDILVG ENDLTALSHL HEPAVLHNLK VRFLESNHIY
110 120 130 140 150
TYCGIVLVAI NPYEQLPIYG QDVIYAYSGQ NMGDMDPHIF AVAEEAYKQM
160 170 180 190 200
ARDEKNQSII VSGESGAGKT VSAKYAMRYF ATVGGSASDT NIEEKVLASS
210 220 230 240 250
PIMEAIGNAK TTRNDNSSRF GKYIEIGFDK KYHIIGANMR TYLLEKSRVV
260 270 280 290 300
FQADDERNYH IFYQLCAAAS LPEFKELALT CAEDFFYTAH GGNTTIEGVD
310 320 330 340 350
DAEDFEKTRQ ALTLLGVRES HQISIFKIIA SILHLGSVEI QAERDGDSCS
360 370 380 390 400
ISPQDEHLSN FCRLLGIEHS QMEHWLCHRK LVTTSETYVK TMSLQQVVNA
410 420 430 440 450
RNALAKHIYA QLFSWIVEHI NKALQTSLKQ HSFIGVLDIY GFETFEINSF
460 470 480 490 500
EQFCINYANE KLQQQFNSHV FKLEQEEYMK EQIPWTLIDF YDNQPCIDLI
510 520 530 540 550
EAKLGILDLL DEECKVPKGT DQNWAQKLYE RHSNSQHFQK PRMSNTAFIV
560 570 580 590 600
IHFADKVEYL SDGFLEKNRD TVYEEQINIL KASKFPLVAD LFRDDEDSVP
610 620 630 640 650
ATNTAKSRSS SKINVRSSRP LMKAPNKEHK KSVGYQFRTS LNLLMETLNA
660 670 680 690 700
TTPHYVRCIK PNDEKLPFHF DPKRAVQQLR ACGVLETIRI SAAGYPSRWT
710 720 730 740 750
YHDFFNRYRV LMKKRELANT TDKKNICKSV LESLIKDPDK FQFGRTKIFF
760 770 780 790 800
RAGQVAYLEK LRADKFREAT IMIQKTVRGW LQRVKYRRLR AATLTLQRFC
810 820 830 840 850
RGYLARRLTE HLRRTRAAIV FQKQYRMLKA RRAYCRVRRA AVIIQSYTRG
860 870 880 890 900
HVCTQKLPPV LTEHKATIIQ KYARGWMARR HFQRQRDAAI VIQCAFRRLK
910 920 930 940 950
ARQALKALKI EARSAEHLKR LNVGMENKVV QLQRKIDDQN KEFKTLSEQL
960 970 980 990 1000
SAVTSTHAME VEKLKKELAR YQQNQEADPS LQLQEEVQSL RTELQKAHSE
1010 1020 1030 1040 1050
RRVLEDAHNR ENGELRKRVA DLEHENALLK DEKEHLNHQI LRQSKAESSQ
1060 1070 1080 1090 1100
SSVEENLLIK KELEEERSRY QNLVKEYSQL EQRYENLRDE QQTPGHRKNP
1110 1120 1130 1140 1150
SNQSSLESDS NYPSISTSEI GDTEDALQQV EEIGIEKAAM DMTVFLKLQK
1160 1170 1180 1190 1200
RVRELEQERK KLQVQLEKEQ QDSKKVQVEQ QNNGLDVDQD ADIAYNSLKR
1210 1220 1230 1240 1250
QELESENKKL KNDLNERWKA VADQAMQDNS THSSPDSYSL LLNQLKLANE
1260 1270 1280 1290 1300
ELEVRKEEVL ILRTQIMNAD QRRLSGKNME PNINARTSWP NSEKHVDQED
1310 1320 1330 1340 1350
AIEAYHGVCQ TNSQTEDWGY LNEDGELGLA YQGLKQVARL LEAQLQAQNL
1360 1370 1380 1390 1400
KHEEEVEHLK AQVEAMKEEM DKQQQTFCQT LLLSPEAQVE FGVQQEISRL
1410 1420 1430 1440 1450
TNENLDFKEL VEKLEKNEKK LKKQLKIYMK KVQDLEAAQA LAQSDRRHHE
1460 1470 1480 1490 1500
LTRQVTVQRK EKDFQGMLEY HKEDEALLIR NLVTDLKPQM LSGTVPCLPA
1510 1520 1530 1540 1550
YILYMCIRHA DYTNDDLKVH SLLSSTINGI KKVLKKHNED FEMTSFWLSN
1560 1570 1580 1590 1600
TCRLLHCLKQ YSGDEGFMTQ NTAKQNEHCL KNFDLTEYRQ VLSDLSIQIY
1610 1620 1630 1640 1650
QQLIKIAEGL LQPMIVSAML ENESIQGLSG VRPTGYRKRS SSMVDGENSY
1660 1670 1680 1690 1700
CLEAIIRQMN FFHTVLCDQG LDPEIILQVF KQLFYMINAV TLNNLLLRKD
1710 1720 1730 1740 1750
ACSWSTGMQL RYNISQLEEW LRGKNLQQSG AVQTMEPLIQ AAQLLQLKKK
1760 1770 1780 1790 1800
TQEDAEAICS LCTSLSTQQI VKILNLYTPL NGFEERVTVS FIRTIQAQLQ
1810 1820 1830 1840
ERSDPQQLLL DSKHMFPVLF PFNPSALTMD SIHIPACLNL EFLNEV
Length:1,846
Mass (Da):213,721
Last modified:February 1, 1997 - v1
Checksum:iAEC1B6F37389EE48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60416 mRNA. Translation: AAB38840.1.
PIRiA59289.
RefSeqiNP_058779.1. NM_017083.1.
UniGeneiRn.10640.

Genome annotation databases

GeneIDi25132.
KEGGirno:25132.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60416 mRNA. Translation: AAB38840.1.
PIRiA59289.
RefSeqiNP_058779.1. NM_017083.1.
UniGeneiRn.10640.

3D structure databases

ProteinModelPortaliP70569.
SMRiP70569. Positions 4-790.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247201. 2 interactions.
IntActiP70569. 8 interactions.
STRINGi10116.ENSRNOP00000019512.

PTM databases

iPTMnetiP70569.
PhosphoSiteiP70569.

Proteomic databases

PaxDbiP70569.
PRIDEiP70569.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25132.
KEGGirno:25132.

Organism-specific databases

CTDi4645.
RGDi621347. Myo5b.

Phylogenomic databases

eggNOGiKOG0160. Eukaryota.
KOG0161. Eukaryota.
COG5022. LUCA.
HOGENOMiHOG000171839.
HOVERGENiHBG052556.
InParanoidiP70569.
KOiK10357.
PhylomeDBiP70569.

Miscellaneous databases

PROiP70569.

Family and domain databases

InterProiIPR002710. Dilute_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01843. DIL. 1 hit.
PF00612. IQ. 6 hits.
PF00063. Myosin_head. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM01132. DIL. 1 hit.
SM00015. IQ. 6 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51126. DILUTE. 1 hit.
PS50096. IQ. 6 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of myr 6, an unconventional myosin of the dilute/myosin-V family."
    Zhao L.P., Koslovsky J.S., Reinhard J., Bahler M., Witt A.E., Provance D.W., Mercer J.A.
    Proc. Natl. Acad. Sci. U.S.A. 93:10826-10831(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Primary structure and cellular localization of chicken brain myosin-V (p190), an unconventional myosin with calmodulin light chains."
    Espreafico E.M., Cheney R.E., Matteoli M., Nascimento A.A., de Camilli P.V., Larson R.E., Mooseker M.S.
    J. Cell Biol. 119:1541-1557(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMYO5B_RAT
AccessioniPrimary (citable) accession number: P70569
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.