P70564 (SPB5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serpin B5

Alternative name(s):
Maspin
Peptidase inhibitor 5
Short name=PI-5

Gene names

Name:	Serpinb5
Synonyms:	Pi5

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity By similarity.
Subunit structure	Interacts with IRF6 By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the serpin family. Ov-serpin subfamily.

Ontologies

Keywords
Cellular component	Secreted
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of proteolysis Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Serpin B5		PRO_0000032488
Sites
	Site	340 – 341	2	Reactive bond homolog By similarity
Amino acid modifications
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	188	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	361	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P70564 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E82C4EC6A0F2E482	FASTA	375	42,063
	10 20 30 40 50 60 MDALRLANSA FAVELFKQLC EKEPAGNILF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF 70 80 90 100 110 120 ENVKDVPFGF KPITSDVNKL SSFYSLKLIK RLYIDKSLNL STEFISSTKR PYANELETVD 130 140 150 160 170 180 FKDKLEETKG QINSSIKELT DGHFEDILPE NSISDQTKIL VVNAAYFVGK WMKKFPESET 190 200 210 220 230 240 KECPFRINKT DTKPVQMMNL EATFCLGNID DINCKIIELP FQNKHLSMLI VLPKDVEDES 250 260 270 280 290 300 TGLEKIEKQL NPETLLQWTN PSTMANAKVK LSLPKFKVEK MIDPKASLES LGLKSLFNES 310 320 330 340 350 360 TSDFSGMSET KGVSVSNVIH RVCLEITEDG GDSIEVPGSR ILQHKDEFKA DHPFLFIVRH 370 NKTRNIVFLG KFSSP

References

[1]	"Rat and human maspins: structures, metastatic suppressor activity and mutation in prostate cancer cells." Umekita Y., Hiipakka R.A., Liao S. Cancer Lett. 113:87-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Vagina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U58857 mRNA. Translation: AAB06043.1.
IPI	IPI00369142.
RefSeq	NP_476449.1. NM_057108.2.
UniGene	Rn.25752.
3D structure databases
ProteinModelPortal	P70564.
SMR	P70564. Positions 1-375.
ModBase	Search...
Protein family/group databases
MEROPS	I04.980.
Proteomic databases
PaxDb	P70564.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	116589.
KEGG	rno:116589.
UCSC	RGD:69342. rat.
Organism-specific databases
CTD	5268.
RGD	69342. Serpinb5.
Phylogenomic databases
eggNOG	COG4826.
HOGENOM	HOG000238519.
HOVERGEN	HBG005957.
InParanoid	P70564.
KO	K10139.
OrthoDB	EOG495ZS0.
Gene expression databases
Genevestigator	P70564.
GermOnline	ENSRNOG00000002640. Rattus norvegicus.
Family and domain databases
InterPro	IPR023795. Protease_inhib_I4_serpin_CS. IPR000240. Serpin_B9/Maspin. IPR023796. Serpin_dom. IPR000215. Serpin_fam. [Graphical view]
PANTHER	PTHR11461. PTHR11461. 1 hit.
Pfam	PF00079. Serpin. 1 hit. [Graphical view]
PRINTS	PR00676. MASPIN.
SMART	SM00093. SERPIN. 1 hit. [Graphical view]
SUPFAM	SSF56574. Prot_inh_serpin. 1 hit.
PROSITE	PS00284. SERPIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	619265.

Entry information

Entry name

SPB5_RAT

Accession

Primary (citable) accession number: P70564

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program