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Protein

Class A basic helix-loop-helix protein 15

Gene

Bhlha15

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in controlling the transcriptional activity of MyoD, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3' (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class A basic helix-loop-helix protein 15
Short name:
bHLHa15
Alternative name(s):
Class B basic helix-loop-helix protein 8
Short name:
bHLHb8
Muscle, intestine and stomach expression 1
Short name:
MIST-1
Gene namesi
Name:Bhlha15
Synonyms:Bhlhb8, Mist1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi3091. Bhlha15.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197Class A basic helix-loop-helix protein 15PRO_0000127152Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121PhosphothreonineBy similarity
Modified residuei25 – 251PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP70562.
PRIDEiP70562.

PTM databases

iPTMnetiP70562.

Expressioni

Tissue specificityi

Expressed in liver, spleen and olfactory epithelium. Weaker expression is seen in skeletal muscle, cardiac muscle, eye and brain tissue.1 Publication

Gene expression databases

GenevisibleiP70562. RN.

Interactioni

Subunit structurei

Forms homodimers or heterodimers with TCF3 gene products E12 and E47. These dimers bind to the E-box site, however, heterodimer with MYOD1 does not bind target DNA.

Protein-protein interaction databases

BioGridi247373. 1 interaction.
STRINGi10116.ENSRNOP00000035219.

Structurei

3D structure databases

ProteinModelPortaliP70562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 12453bHLHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi152 – 18938Gln-richAdd
BLAST

Domaini

Lacks a classic transcription activation domain and instead possesses an N-terminal region capable of inhibiting heterologous activators.By similarity

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IQ4H. Eukaryota.
ENOG4111ZMK. LUCA.
GeneTreeiENSGT00680000099860.
HOGENOMiHOG000095227.
InParanoidiP70562.
KOiK08040.
OMAiPEPAKGL.
OrthoDBiEOG7GQXWR.
PhylomeDBiP70562.
TreeFamiTF315153.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTKNRPPRR RTPMQDAEAT PGEQTPDRSQ SGSGASEVTK GLRSRTARAS
60 70 80 90 100
GTRAEVSRRR QGSSSRRENS VQRRLESNER ERQRMHKLNN AFQALREVIP
110 120 130 140 150
HVRADKKLSK IETLTLAKNY IKSLTATILT MSSSRLPGLE APGPAPGPKL
160 170 180 190
YQHYHHQQQQ QQQQQQVAGA VLGVTEDQPQ GHLQRYSTQI HSFREGS
Length:197
Mass (Da):22,196
Last modified:February 1, 1997 - v1
Checksum:i067AEAC33B5D49CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58279 mRNA. Translation: AAC53111.1.
AF049874 Genomic DNA. Translation: AAF17707.1.
BC061868 mRNA. Translation: AAH61868.1.
RefSeqiNP_036995.1. NM_012863.1.
UniGeneiRn.9897.

Genome annotation databases

EnsembliENSRNOT00000037251; ENSRNOP00000035219; ENSRNOG00000025164.
GeneIDi25334.
KEGGirno:25334.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58279 mRNA. Translation: AAC53111.1.
AF049874 Genomic DNA. Translation: AAF17707.1.
BC061868 mRNA. Translation: AAH61868.1.
RefSeqiNP_036995.1. NM_012863.1.
UniGeneiRn.9897.

3D structure databases

ProteinModelPortaliP70562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247373. 1 interaction.
STRINGi10116.ENSRNOP00000035219.

PTM databases

iPTMnetiP70562.

Proteomic databases

PaxDbiP70562.
PRIDEiP70562.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000037251; ENSRNOP00000035219; ENSRNOG00000025164.
GeneIDi25334.
KEGGirno:25334.

Organism-specific databases

CTDi168620.
RGDi3091. Bhlha15.

Phylogenomic databases

eggNOGiENOG410IQ4H. Eukaryota.
ENOG4111ZMK. LUCA.
GeneTreeiENSGT00680000099860.
HOGENOMiHOG000095227.
InParanoidiP70562.
KOiK08040.
OMAiPEPAKGL.
OrthoDBiEOG7GQXWR.
PhylomeDBiP70562.
TreeFamiTF315153.

Miscellaneous databases

PROiP70562.

Gene expression databases

GenevisibleiP70562. RN.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mist1: a novel basic helix-loop-helix transcription factor exhibits a developmentally regulated expression pattern."
    Lemercier C., To R.Q., Swanson B.J., Lyons G.E., Konieczny S.F.
    Dev. Biol. 182:101-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, CHARACTERIZATION.
    Strain: Sprague-Dawley.
    Tissue: Olfactory epithelium.
  2. "The rat Mist1 gene: structure and promoter characterization."
    Lemercier C., Brown A., Mamani M., Ripoche J., Reiffers J.
    Gene 242:209-218(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-25, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBHA15_RAT
AccessioniPrimary (citable) accession number: P70562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: February 1, 1997
Last modified: June 8, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.