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Protein

Class A basic helix-loop-helix protein 15

Gene

Bhlha15

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in controlling the transcriptional activity of MyoD, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3' (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Class A basic helix-loop-helix protein 15
Short name:
bHLHa15
Alternative name(s):
Class B basic helix-loop-helix protein 8
Short name:
bHLHb8
Muscle, intestine and stomach expression 1
Short name:
MIST-1
Gene namesi
Name:Bhlha15
Synonyms:Bhlhb8, Mist1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi3091 Bhlha15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271521 – 197Class A basic helix-loop-helix protein 15Add BLAST197

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphothreonineBy similarity1
Modified residuei25PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP70562
PRIDEiP70562

PTM databases

iPTMnetiP70562
PhosphoSitePlusiP70562

Expressioni

Tissue specificityi

Expressed in liver, spleen and olfactory epithelium. Weaker expression is seen in skeletal muscle, cardiac muscle, eye and brain tissue.1 Publication

Gene expression databases

BgeeiENSRNOG00000025164
GenevisibleiP70562 RN

Interactioni

Subunit structurei

Forms homodimers or heterodimers with TCF3 gene products E12 and E47. These dimers bind to the E-box site, however, heterodimer with MYOD1 does not bind target DNA.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247373, 1 interactor
STRINGi10116.ENSRNOP00000035219

Structurei

3D structure databases

ProteinModelPortaliP70562
SMRiP70562
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 124bHLHPROSITE-ProRule annotationAdd BLAST53

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi152 – 189Gln-richAdd BLAST38

Domaini

Lacks a classic transcription activation domain and instead possesses an N-terminal region capable of inhibiting heterologous activators.By similarity

Phylogenomic databases

eggNOGiENOG410IQ4H Eukaryota
ENOG4111ZMK LUCA
GeneTreeiENSGT00680000099860
HOGENOMiHOG000095227
InParanoidiP70562
KOiK08040
OMAiHRYSTQI
OrthoDBiEOG091G15AI
PhylomeDBiP70562
TreeFamiTF315153

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

P70562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTKNRPPRR RTPMQDAEAT PGEQTPDRSQ SGSGASEVTK GLRSRTARAS
60 70 80 90 100
GTRAEVSRRR QGSSSRRENS VQRRLESNER ERQRMHKLNN AFQALREVIP
110 120 130 140 150
HVRADKKLSK IETLTLAKNY IKSLTATILT MSSSRLPGLE APGPAPGPKL
160 170 180 190
YQHYHHQQQQ QQQQQQVAGA VLGVTEDQPQ GHLQRYSTQI HSFREGS
Length:197
Mass (Da):22,196
Last modified:February 1, 1997 - v1
Checksum:i067AEAC33B5D49CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58279 mRNA Translation: AAC53111.1
AF049874 Genomic DNA Translation: AAF17707.1
BC061868 mRNA Translation: AAH61868.1
RefSeqiNP_036995.1, NM_012863.1
UniGeneiRn.9897

Genome annotation databases

EnsembliENSRNOT00000037251; ENSRNOP00000035219; ENSRNOG00000025164
GeneIDi25334
KEGGirno:25334

Similar proteinsi

Entry informationi

Entry nameiBHA15_RAT
AccessioniPrimary (citable) accession number: P70562
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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