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Protein

GTP cyclohydrolase 1 feedback regulatory protein

Gene

Gchfr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase 1. This inhibition is reversed by L-phenylalanine.

GO - Molecular functioni

  • amino acid binding Source: RGD
  • enzyme binding Source: RGD
  • enzyme inhibitor activity Source: RGD
  • GTP cyclohydrolase binding Source: RGD
  • GTP-dependent protein binding Source: RGD

GO - Biological processi

  • negative regulation of biosynthetic process Source: InterPro
  • negative regulation of GTP cyclohydrolase I activity Source: RGD
  • protein heterooligomerization Source: RGD
  • regulation of nitric oxide biosynthetic process Source: RGD

Enzyme and pathway databases

ReactomeiR-RNO-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase 1 feedback regulatory protein
Short name:
GFRP
Alternative name(s):
GTP cyclohydrolase I feedback regulatory protein
p35
Gene namesi
Name:Gchfr
Synonyms:Gfrp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621746 Gchfr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001896771 – 84GTP cyclohydrolase 1 feedback regulatory proteinAdd BLAST84

Proteomic databases

PaxDbiP70552
PRIDEiP70552

PTM databases

iPTMnetiP70552
PhosphoSitePlusiP70552

Expressioni

Tissue specificityi

Liver and kidney. Somewhat lower level in testis, heart, brain and lung.

Gene expression databases

BgeeiENSRNOG00000012290
GenevisibleiP70552 RN

Interactioni

Subunit structurei

Homopentamer. Forms a complex with GCH1 where a GCH1 homodecamer is sandwiched by two GFRP homopentamers. Interacts with GCH1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gch1P222883EBI-1032715,EBI-1032708

GO - Molecular functioni

  • enzyme binding Source: RGD
  • GTP cyclohydrolase binding Source: RGD
  • GTP-dependent protein binding Source: RGD

Protein-protein interaction databases

BioGridi251135, 2 interactors
CORUMiP70552
IntActiP70552, 1 interactor
STRINGi10116.ENSRNOP00000016458

Structurei

Secondary structure

184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Beta strandi12 – 14Combined sources3
Beta strandi17 – 20Combined sources4
Helixi26 – 31Combined sources6
Beta strandi35 – 37Combined sources3
Beta strandi46 – 51Combined sources6
Helixi53 – 62Combined sources10
Beta strandi66 – 73Combined sources8
Beta strandi76 – 82Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IS7X-ray2.80K/L/M/N/O/P/Q/R/S/T1-84[»]
1IS8X-ray2.70K/L/M/N/O/P/Q/R/S/T1-84[»]
1JG5X-ray2.60A/B/C/D/E2-84[»]
1WPLX-ray2.80K/L/M/N/O/P/Q/R/S/T1-84[»]
ProteinModelPortaliP70552
SMRiP70552
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70552

Family & Domainsi

Sequence similaritiesi

Belongs to the GFRP family.Curated

Phylogenomic databases

eggNOGiENOG410J0GT Eukaryota
ENOG4111X10 LUCA
GeneTreeiENSGT00440000033849
HOGENOMiHOG000008074
HOVERGENiHBG000233
InParanoidiP70552
OMAiTMVGDEY
OrthoDBiEOG091G15IO
PhylomeDBiP70552
TreeFamiTF329303

Family and domain databases

Gene3Di3.30.1410.10, 1 hit
InterProiView protein in InterPro
IPR036717 GFRP_sf
IPR009112 GTP_CycHdrlase_I_reg
PANTHERiPTHR16852 PTHR16852, 1 hit
PfamiView protein in Pfam
PF06399 GFRP, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD028559 GTP_CycHdrlase_I_reg, 1 hit
SUPFAMiSSF69761 SSF69761, 1 hit

Sequencei

Sequence statusi: Complete.

P70552-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYLLISTQI RMEVGPTMVG DEHSDPELMQ QLGASKRRVL GNNFYEYYVN
60 70 80
DPPRIVLDKL ECRGFRVLSM TGVGQTLVWC LHKE
Length:84
Mass (Da):9,672
Last modified:January 23, 2007 - v3
Checksum:i874EC3B28EC7DDFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53710 mRNA Translation: AAC52776.1
U85512 mRNA Translation: AAD09241.1
BC086944 mRNA Translation: AAH86944.1
RefSeqiNP_598279.1, NM_133595.2
UniGeneiRn.11137

Genome annotation databases

EnsembliENSRNOT00000016458; ENSRNOP00000016458; ENSRNOG00000012290
GeneIDi171128
KEGGirno:171128
UCSCiRGD:621746 rat

Similar proteinsi

Entry informationi

Entry nameiGFRP_RAT
AccessioniPrimary (citable) accession number: P70552
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health