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Protein

GTP cyclohydrolase 1 feedback regulatory protein

Gene

Gchfr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase 1. This inhibition is reversed by L-phenylalanine.

GO - Molecular functioni

  • amino acid binding Source: RGD
  • enzyme binding Source: RGD
  • enzyme inhibitor activity Source: RGD
  • GTP cyclohydrolase binding Source: RGD
  • GTP-dependent protein binding Source: RGD

GO - Biological processi

  • negative regulation of biosynthetic process Source: InterPro
  • negative regulation of GTP cyclohydrolase I activity Source: RGD
  • protein complex assembly Source: RGD
  • protein heterooligomerization Source: RGD
  • regulation of nitric oxide biosynthetic process Source: RGD
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase 1 feedback regulatory protein
Short name:
GFRP
Alternative name(s):
GTP cyclohydrolase I feedback regulatory protein
p35
Gene namesi
Name:Gchfr
Synonyms:Gfrp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621746. Gchfr.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • dendrite Source: Ensembl
  • melanosome Source: Ensembl
  • nuclear membrane Source: UniProtKB-SubCell
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001896772 – 84GTP cyclohydrolase 1 feedback regulatory proteinAdd BLAST83

Proteomic databases

PaxDbiP70552.
PRIDEiP70552.

PTM databases

iPTMnetiP70552.
PhosphoSitePlusiP70552.

Expressioni

Tissue specificityi

Liver and kidney. Somewhat lower level in testis, heart, brain and lung.

Gene expression databases

BgeeiENSRNOG00000012290.
GenevisibleiP70552. RN.

Interactioni

Subunit structurei

Homopentamer. Forms a complex with GCH1 where a GCH1 homodecamer is sandwiched by two GFRP homopentamers. Interacts with GCH1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gch1P222883EBI-1032715,EBI-1032708

GO - Molecular functioni

  • enzyme binding Source: RGD
  • GTP cyclohydrolase binding Source: RGD
  • GTP-dependent protein binding Source: RGD

Protein-protein interaction databases

BioGridi251135. 2 interactors.
IntActiP70552. 1 interactor.
MINTiMINT-4570049.
STRINGi10116.ENSRNOP00000016458.

Structurei

Secondary structure

184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Beta strandi12 – 14Combined sources3
Beta strandi17 – 20Combined sources4
Helixi26 – 31Combined sources6
Beta strandi35 – 37Combined sources3
Beta strandi46 – 51Combined sources6
Helixi53 – 62Combined sources10
Beta strandi66 – 73Combined sources8
Beta strandi76 – 82Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IS7X-ray2.80K/L/M/N/O/P/Q/R/S/T1-84[»]
1IS8X-ray2.70K/L/M/N/O/P/Q/R/S/T1-84[»]
1JG5X-ray2.60A/B/C/D/E2-84[»]
1WPLX-ray2.80K/L/M/N/O/P/Q/R/S/T1-84[»]
ProteinModelPortaliP70552.
SMRiP70552.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70552.

Family & Domainsi

Sequence similaritiesi

Belongs to the GFRP family.Curated

Phylogenomic databases

eggNOGiENOG410J0GT. Eukaryota.
ENOG4111X10. LUCA.
GeneTreeiENSGT00440000033849.
HOGENOMiHOG000008074.
HOVERGENiHBG000233.
InParanoidiP70552.
OMAiTMVGDEY.
OrthoDBiEOG091G15IO.
PhylomeDBiP70552.
TreeFamiTF329303.

Family and domain databases

Gene3Di3.30.1410.10. 1 hit.
InterProiIPR009112. GTP_CycHdrlase_I_reg.
[Graphical view]
PfamiPF06399. GFRP. 1 hit.
[Graphical view]
ProDomiPD028559. GTP_CycHdrlase_I_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF69761. SSF69761. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70552-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYLLISTQI RMEVGPTMVG DEHSDPELMQ QLGASKRRVL GNNFYEYYVN
60 70 80
DPPRIVLDKL ECRGFRVLSM TGVGQTLVWC LHKE
Length:84
Mass (Da):9,672
Last modified:January 23, 2007 - v3
Checksum:i874EC3B28EC7DDFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53710 mRNA. Translation: AAC52776.1.
U85512 mRNA. Translation: AAD09241.1.
BC086944 mRNA. Translation: AAH86944.1.
RefSeqiNP_598279.1. NM_133595.2.
UniGeneiRn.11137.

Genome annotation databases

EnsembliENSRNOT00000016458; ENSRNOP00000016458; ENSRNOG00000012290.
GeneIDi171128.
KEGGirno:171128.
UCSCiRGD:621746. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53710 mRNA. Translation: AAC52776.1.
U85512 mRNA. Translation: AAD09241.1.
BC086944 mRNA. Translation: AAH86944.1.
RefSeqiNP_598279.1. NM_133595.2.
UniGeneiRn.11137.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IS7X-ray2.80K/L/M/N/O/P/Q/R/S/T1-84[»]
1IS8X-ray2.70K/L/M/N/O/P/Q/R/S/T1-84[»]
1JG5X-ray2.60A/B/C/D/E2-84[»]
1WPLX-ray2.80K/L/M/N/O/P/Q/R/S/T1-84[»]
ProteinModelPortaliP70552.
SMRiP70552.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251135. 2 interactors.
IntActiP70552. 1 interactor.
MINTiMINT-4570049.
STRINGi10116.ENSRNOP00000016458.

PTM databases

iPTMnetiP70552.
PhosphoSitePlusiP70552.

Proteomic databases

PaxDbiP70552.
PRIDEiP70552.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016458; ENSRNOP00000016458; ENSRNOG00000012290.
GeneIDi171128.
KEGGirno:171128.
UCSCiRGD:621746. rat.

Organism-specific databases

CTDi2644.
RGDi621746. Gchfr.

Phylogenomic databases

eggNOGiENOG410J0GT. Eukaryota.
ENOG4111X10. LUCA.
GeneTreeiENSGT00440000033849.
HOGENOMiHOG000008074.
HOVERGENiHBG000233.
InParanoidiP70552.
OMAiTMVGDEY.
OrthoDBiEOG091G15IO.
PhylomeDBiP70552.
TreeFamiTF329303.

Enzyme and pathway databases

ReactomeiR-RNO-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

Miscellaneous databases

EvolutionaryTraceiP70552.
PROiP70552.

Gene expression databases

BgeeiENSRNOG00000012290.
GenevisibleiP70552. RN.

Family and domain databases

Gene3Di3.30.1410.10. 1 hit.
InterProiIPR009112. GTP_CycHdrlase_I_reg.
[Graphical view]
PfamiPF06399. GFRP. 1 hit.
[Graphical view]
ProDomiPD028559. GTP_CycHdrlase_I_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF69761. SSF69761. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGFRP_RAT
AccessioniPrimary (citable) accession number: P70552
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.