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Protein

Type II iodothyronine deiodinase

Gene

Dio2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.

Catalytic activityi

3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei130 – 1301

GO - Molecular functioni

  • thyroxine 5'-deiodinase activity Source: RGD

GO - Biological processi

  • hormone biosynthetic process Source: UniProtKB-KW
  • response to cold Source: RGD
  • response to lipopolysaccharide Source: RGD
  • thyroid hormone generation Source: RGD
  • thyroid hormone metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Thyroid hormones biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Type II iodothyronine deiodinase (EC:1.97.1.10)
Alternative name(s):
5DII
DIOII
Type 2 DI
Type-II 5'-deiodinase
Gene namesi
Name:Dio2
Synonyms:Itdi2, Txdi2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68418. Dio2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3425HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Type II iodothyronine deiodinasePRO_0000154319Add
BLAST

Post-translational modificationi

Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33 (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Expressioni

Tissue specificityi

Expressed in cerebral cortex, cerebellum, pituitary gland, mostly in anterior pituitary gland, and pineal gland, as well as in brown adipose tissue (BAT).3 Publications

Inductioni

In the pineal gland, exhibits night/day variations with a 9-fold increased expression at night. Up-regulation is due to a large degree to the release of norepinephrine from nerve terminals in the pineal gland and cAMP signaling pathway. In BAT, up-regulated in animals exposed to cold.3 Publications

Gene expression databases

GenevisibleiP70551. RN.

Interactioni

Subunit structurei

Interacts with USP20 and USP33. Interacts with MARCH6 (By similarity).By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the iodothyronine deiodinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG146259.
HOGENOMiHOG000007088.
HOVERGENiHBG000099.
InParanoidiP70551.
KOiK17904.
PhylomeDBiP70551.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11781. PTHR11781. 1 hit.
PfamiPF00837. T4_deiodinase. 1 hit.
[Graphical view]
PIRSFiPIRSF001330. IOD. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVALL LSRSKSTRGE
60 70 80 90 100
WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPEAGNNCAS
110 120 130 140 150
EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV
160 170 180 190 200
ADFLLVYIDE AHPSDGWAVP GDSSMSFEVK KHRNQEDRCA AAHQLLERFS
210 220 230 240 250
LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE
260
VRSWLEKNFS KRUILD
Length:266
Mass (Da):29,871
Last modified:February 26, 2008 - v4
Checksum:i06DE3292FB467578
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti116 – 1161S → C in BAA25186 (PubMed:9709916).Curated

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei130 – 1301Selenocysteine
Non-standard residuei263 – 2631Selenocysteine

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53505 mRNA. Translation: AAC52767.1.
AB011068 mRNA. Translation: BAA25186.1.
PIRiT10816.
RefSeqiNP_113908.3. NM_031720.3.
UniGeneiRn.88380.

Genome annotation databases

GeneIDi65162.
KEGGirno:65162.
UCSCiRGD:68418. rat.

Keywords - Coding sequence diversityi

Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53505 mRNA. Translation: AAC52767.1.
AB011068 mRNA. Translation: BAA25186.1.
PIRiT10816.
RefSeqiNP_113908.3. NM_031720.3.
UniGeneiRn.88380.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65162.
KEGGirno:65162.
UCSCiRGD:68418. rat.

Organism-specific databases

CTDi1734.
RGDi68418. Dio2.

Phylogenomic databases

eggNOGiNOG146259.
HOGENOMiHOG000007088.
HOVERGENiHBG000099.
InParanoidiP70551.
KOiK17904.
PhylomeDBiP70551.

Miscellaneous databases

NextBioi614031.
PROiP70551.

Gene expression databases

GenevisibleiP70551. RN.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11781. PTHR11781. 1 hit.
PfamiPF00837. T4_deiodinase. 1 hit.
[Graphical view]
PIRSFiPIRSF001330. IOD. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of the mammalian type II iodothyronine deiodinase. A selenoprotein differentially expressed and regulated in human and rat brain and other tissues."
    Croteau W., Davey J.C., Galton V.A., St Germain D.L.
    J. Clin. Invest. 98:405-417(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: Sprague-Dawley.
    Tissue: Brown adipose tissue.
  2. "Induction of type 2 deiodinase activity by cyclic guanosine 3',5'-monophosphate in cultured rat glial cells."
    Gondou A., Toyoda N., Nishikawa M., Tabata S., Yonemoto T., Ogawa Y., Tokoro T., Sakaguchi N., Wang F., Inada M.
    Thyroid 8:615-622(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Expression and nocturnal increase of type II iodothyronine deiodinase mRNA in rat pineal gland."
    Murakami M., Hosoi Y., Negishi T., Kamiya Y., Ogiwara T., Mizuma H., Yamada M., Iriuchijima T., Mori M.
    Neurosci. Lett. 227:65-67(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  4. Cited for: TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiIOD2_RAT
AccessioniPrimary (citable) accession number: P70551
Secondary accession number(s): O70179
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 26, 2008
Last modified: June 24, 2015
This is version 114 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.