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Protein

Type II iodothyronine deiodinase

Gene

Dio2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.

Catalytic activityi

3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1301

GO - Molecular functioni

  • thyroxine 5'-deiodinase activity Source: RGD

GO - Biological processi

  • hormone biosynthetic process Source: UniProtKB-KW
  • response to cold Source: RGD
  • response to lipopolysaccharide Source: RGD
  • thyroid hormone generation Source: RGD
  • thyroid hormone metabolic process Source: RGD

Keywordsi

Molecular functionOxidoreductase
Biological processThyroid hormones biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Type II iodothyronine deiodinase (EC:1.21.99.4)
Alternative name(s):
5DII
DIOII
Type 2 DI
Type-II 5'-deiodinase
Gene namesi
Name:Dio2
Synonyms:Itdi2, Txdi2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68418 Dio2

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei10 – 34HelicalSequence analysisAdd BLAST25

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001543191 – 266Type II iodothyronine deiodinaseAdd BLAST266

Post-translational modificationi

Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33 (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiP70551

Expressioni

Tissue specificityi

Expressed in cerebral cortex, cerebellum, pituitary gland, mostly in anterior pituitary gland, and pineal gland, as well as in brown adipose tissue (BAT).3 Publications

Inductioni

In the pineal gland, exhibits night/day variations with a 9-fold increased expression at night. Up-regulation is due to a large degree to the release of norepinephrine from nerve terminals in the pineal gland and cAMP signaling pathway. In BAT, up-regulated in animals exposed to cold.3 Publications

Interactioni

Subunit structurei

Interacts with USP20 and USP33. Interacts with MARCH6 (By similarity).By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the iodothyronine deiodinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000007088
HOVERGENiHBG000099
InParanoidiP70551
KOiK17904
PhylomeDBiP70551

Family and domain databases

InterProiView protein in InterPro
IPR000643 Iodothyronine_deiodinase
IPR008261 Iodothyronine_deiodinase_AS
IPR036249 Thioredoxin-like_sf
PANTHERiPTHR11781 PTHR11781, 1 hit
PfamiView protein in Pfam
PF00837 T4_deiodinase, 1 hit
PIRSFiPIRSF001330 IOD, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS01205 T4_DEIODINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P70551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVALL LSRSKSTRGE
60 70 80 90 100
WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPEAGNNCAS
110 120 130 140 150
EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV
160 170 180 190 200
ADFLLVYIDE AHPSDGWAVP GDSSMSFEVK KHRNQEDRCA AAHQLLERFS
210 220 230 240 250
LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE
260
VRSWLEKNFS KRUILD
Length:266
Mass (Da):29,871
Last modified:February 26, 2008 - v4
Checksum:i06DE3292FB467578
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116S → C in BAA25186 (PubMed:9709916).Curated1

Non-standard residue

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-standard residuei130Selenocysteine1
Non-standard residuei263Selenocysteine1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53505 mRNA Translation: AAC52767.1
AB011068 mRNA Translation: BAA25186.1
PIRiT10816
RefSeqiNP_113908.3, NM_031720.4
UniGeneiRn.88380

Genome annotation databases

GeneIDi65162
KEGGirno:65162
UCSCiRGD:68418 rat

Keywords - Coding sequence diversityi

Selenocysteine

Similar proteinsi

Entry informationi

Entry nameiIOD2_RAT
AccessioniPrimary (citable) accession number: P70551
Secondary accession number(s): O70179
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 26, 2008
Last modified: May 23, 2018
This is version 123 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health