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P70551

- IOD2_RAT

UniProt

P70551 - IOD2_RAT

Protein

Type II iodothyronine deiodinase

Gene

Dio2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 4 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.

    Catalytic activityi

    3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei130 – 1301

    GO - Molecular functioni

    1. thyroxine 5'-deiodinase activity Source: RGD

    GO - Biological processi

    1. hormone biosynthetic process Source: UniProtKB-KW
    2. response to cold Source: RGD
    3. response to lipopolysaccharide Source: RGD
    4. thyroid hormone generation Source: RGD
    5. thyroid hormone metabolic process Source: RGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Thyroid hormones biosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type II iodothyronine deiodinase (EC:1.97.1.10)
    Alternative name(s):
    5DII
    DIOII
    Type 2 DI
    Type-II 5'-deiodinase
    Gene namesi
    Name:Dio2
    Synonyms:Itdi2, Txdi2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi68418. Dio2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 266266Type II iodothyronine deiodinasePRO_0000154319Add
    BLAST

    Post-translational modificationi

    Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33 By similarity.By similarity

    Keywords - PTMi

    Ubl conjugation

    Expressioni

    Tissue specificityi

    Expressed in cerebral cortex, cerebellum, pituitary gland, mostly in anterior pituitary gland, and pineal gland, as well as in brown adipose tissue (BAT).3 Publications

    Inductioni

    In the pineal gland, exhibits night/day variations with a 9-fold increased expression at night. Up-regulation is due to a large degree to the release of norepinephrine from nerve terminals in the pineal gland and cAMP signaling pathway. In BAT, up-regulated in animals exposed to cold.3 Publications

    Gene expression databases

    GenevestigatoriP70551.

    Interactioni

    Subunit structurei

    Interacts with USP20 and USP33. Interacts with MARCH6 By similarity.By similarity

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei10 – 3425HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the iodothyronine deiodinase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG146259.
    HOGENOMiHOG000007088.
    HOVERGENiHBG000099.
    InParanoidiP70551.
    KOiK17904.
    PhylomeDBiP70551.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR000643. Iodothyronine_deiodinase.
    IPR008261. Iodothyronine_deiodinase_AS.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR11781. PTHR11781. 1 hit.
    PfamiPF00837. T4_deiodinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 1 hit.
    PROSITEiPS01205. T4_DEIODINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P70551-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLLSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVALL LSRSKSTRGE    50
    WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPEAGNNCAS 100
    EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV 150
    ADFLLVYIDE AHPSDGWAVP GDSSMSFEVK KHRNQEDRCA AAHQLLERFS 200
    LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE 250
    VRSWLEKNFS KRUILD 266
    Length:266
    Mass (Da):29,871
    Last modified:February 26, 2008 - v4
    Checksum:i06DE3292FB467578
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti116 – 1161S → C in BAA25186. (PubMed:9709916)Curated

    Non-standard residue

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-standard residuei130 – 1301Selenocysteine
    Non-standard residuei263 – 2631Selenocysteine

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53505 mRNA. Translation: AAC52767.1.
    AB011068 mRNA. Translation: BAA25186.1.
    PIRiT10816.
    RefSeqiNP_113908.3. NM_031720.3.
    UniGeneiRn.88380.

    Genome annotation databases

    GeneIDi65162.
    KEGGirno:65162.
    UCSCiRGD:68418. rat.

    Keywords - Coding sequence diversityi

    Selenocysteine

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53505 mRNA. Translation: AAC52767.1 .
    AB011068 mRNA. Translation: BAA25186.1 .
    PIRi T10816.
    RefSeqi NP_113908.3. NM_031720.3.
    UniGenei Rn.88380.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 65162.
    KEGGi rno:65162.
    UCSCi RGD:68418. rat.

    Organism-specific databases

    CTDi 1734.
    RGDi 68418. Dio2.

    Phylogenomic databases

    eggNOGi NOG146259.
    HOGENOMi HOG000007088.
    HOVERGENi HBG000099.
    InParanoidi P70551.
    KOi K17904.
    PhylomeDBi P70551.

    Miscellaneous databases

    NextBioi 614031.
    PROi P70551.

    Gene expression databases

    Genevestigatori P70551.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR000643. Iodothyronine_deiodinase.
    IPR008261. Iodothyronine_deiodinase_AS.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    PANTHERi PTHR11781. PTHR11781. 1 hit.
    Pfami PF00837. T4_deiodinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 1 hit.
    PROSITEi PS01205. T4_DEIODINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the mammalian type II iodothyronine deiodinase. A selenoprotein differentially expressed and regulated in human and rat brain and other tissues."
      Croteau W., Davey J.C., Galton V.A., St Germain D.L.
      J. Clin. Invest. 98:405-417(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
      Strain: Sprague-Dawley.
      Tissue: Brown adipose tissue.
    2. "Induction of type 2 deiodinase activity by cyclic guanosine 3',5'-monophosphate in cultured rat glial cells."
      Gondou A., Toyoda N., Nishikawa M., Tabata S., Yonemoto T., Ogawa Y., Tokoro T., Sakaguchi N., Wang F., Inada M.
      Thyroid 8:615-622(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Expression and nocturnal increase of type II iodothyronine deiodinase mRNA in rat pineal gland."
      Murakami M., Hosoi Y., Negishi T., Kamiya Y., Ogiwara T., Mizuma H., Yamada M., Iriuchijima T., Mori M.
      Neurosci. Lett. 227:65-67(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    4. Cited for: TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiIOD2_RAT
    AccessioniPrimary (citable) accession number: P70551
    Secondary accession number(s): O70179
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 111 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3