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P70551 (IOD2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type II iodothyronine deiodinase

EC=1.97.1.10
Alternative name(s):
5DII
DIOII
Type 2 DI
Type-II 5'-deiodinase
Gene names
Name:Dio2
Synonyms:Itdi2, Txdi2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.

Catalytic activity

3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.

Subunit structure

Interacts with USP20 and USP33. Interacts with MARCH6 By similarity.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Tissue specificity

Expressed in cerebral cortex, cerebellum, pituitary gland, mostly in anterior pituitary gland, and pineal gland, as well as in brown adipose tissue (BAT). Ref.1 Ref.3 Ref.4

Induction

In the pineal gland, exhibits night/day variations with a 9-fold increased expression at night. Up-regulation is due to a large degree to the release of norepinephrine from nerve terminals in the pineal gland and cAMP signaling pathway. In BAT, up-regulated in animals exposed to cold. Ref.1 Ref.3 Ref.4

Post-translational modification

Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33 By similarity.

Sequence similarities

Belongs to the iodothyronine deiodinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 266266Type II iodothyronine deiodinase
PRO_0000154319

Regions

Transmembrane10 – 3425Helical; Potential

Sites

Active site1301

Amino acid modifications

Non-standard residue1301Selenocysteine
Non-standard residue2631Selenocysteine

Experimental info

Sequence conflict1161S → C in BAA25186. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P70551 [UniParc].

Last modified February 26, 2008. Version 4.
Checksum: 06DE3292FB467578

FASTA26629,871
        10         20         30         40         50         60 
MGLLSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVALL LSRSKSTRGE WRRMLTSEGL 

        70         80         90        100        110        120 
RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPEAGNNCAS EKTADGAECH LLDFASAERP 

       130        140        150        160        170        180 
LVVNFGSATU PPFTRQLPAF RQLVEEFSSV ADFLLVYIDE AHPSDGWAVP GDSSMSFEVK 

       190        200        210        220        230        240 
KHRNQEDRCA AAHQLLERFS LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG 

       250        260 
KGPFSYNLQE VRSWLEKNFS KRUILD 

« Hide

References

[1]"Cloning of the mammalian type II iodothyronine deiodinase. A selenoprotein differentially expressed and regulated in human and rat brain and other tissues."
Croteau W., Davey J.C., Galton V.A., St Germain D.L.
J. Clin. Invest. 98:405-417(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Strain: Sprague-Dawley.
Tissue: Brown adipose tissue.
[2]"Induction of type 2 deiodinase activity by cyclic guanosine 3',5'-monophosphate in cultured rat glial cells."
Gondou A., Toyoda N., Nishikawa M., Tabata S., Yonemoto T., Ogawa Y., Tokoro T., Sakaguchi N., Wang F., Inada M.
Thyroid 8:615-622(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Expression and nocturnal increase of type II iodothyronine deiodinase mRNA in rat pineal gland."
Murakami M., Hosoi Y., Negishi T., Kamiya Y., Ogiwara T., Mizuma H., Yamada M., Iriuchijima T., Mori M.
Neurosci. Lett. 227:65-67(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[4]"Night/day changes in pineal expression of >600 genes: central role of adrenergic/cAMP signaling."
Bailey M.J., Coon S.L., Carter D.A., Humphries A., Kim J.S., Shi Q., Gaildrat P., Morin F., Ganguly S., Hogenesch J.B., Weller J.L., Rath M.F., Moller M., Baler R., Sugden D., Rangel Z.G., Munson P.J., Klein D.C.
J. Biol. Chem. 284:7606-7622(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U53505 mRNA. Translation: AAC52767.1.
AB011068 mRNA. Translation: BAA25186.1.
PIRT10816.
RefSeqNP_113908.3. NM_031720.3.
UniGeneRn.88380.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID65162.
KEGGrno:65162.
UCSCRGD:68418. rat.

Organism-specific databases

CTD1734.
RGD68418. Dio2.

Phylogenomic databases

eggNOGNOG146259.
HOGENOMHOG000007088.
HOVERGENHBG000099.
InParanoidP70551.
KOK17904.
PhylomeDBP70551.

Gene expression databases

GenevestigatorP70551.

Family and domain databases

Gene3D3.40.30.10. 1 hit.
InterProIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERPTHR11781. PTHR11781. 1 hit.
PfamPF00837. T4_deiodinase. 1 hit.
[Graphical view]
SUPFAMSSF52833. SSF52833. 1 hit.
PROSITEPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio614031.
PROP70551.

Entry information

Entry nameIOD2_RAT
AccessionPrimary (citable) accession number: P70551
Secondary accession number(s): O70179
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 26, 2008
Last modified: April 16, 2014
This is version 109 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families