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P70550 (RAB8B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-8B
Gene names
Name:Rab8b
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may be involved in polarized vesicular trafficking and neurotransmitter release. May participate in cell junction dynamics in Sertoli cells. Ref.3

Subunit structure

Associated with actin, delta-catenin and alpha and beta tubulins. Interacts with OTOF By similarity. Interacts with PEX5R. Interacts with RAB3IP By similarity. Interacts with VIM. Interacts with CDH1. Interacts with MICALL2 By similarity. Ref.2 Ref.3

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Cytoplasmic vesiclephagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Recruited to phagosomes containing S.aureus or Mycobacterium By similarity. Colocalizes with CDH1 in the basal compartment.

Tissue specificity

Hight levels of expression in the spleen, heart, kidney, testis and brain. Expression increases in Sertoli and germ cells during their maturation. Ref.3

Developmental stage

Localizes in the basal compartment associating with spermatocytes in all stages of the spermatogenic cycle. Detected at the site of elongate spermatids in stages XII-XIV in addition to being found in the basal compartment in these stages. Ref.3

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCell membrane
Cytoplasmic vesicle
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMLipoprotein
Methylation
Phosphoprotein
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadherens junction organization

Inferred from direct assay Ref.3. Source: UniProtKB

positive regulation of cell projection organization

Inferred from direct assay Ref.2. Source: RGD

positive regulation of corticotropin secretion

Inferred from direct assay Ref.2. Source: RGD

protein import into peroxisome membrane

Inferred from electronic annotation. Source: Ensembl

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcell tip

Inferred from direct assay Ref.2. Source: RGD

cytoplasmic vesicle

Inferred from direct assay Ref.1. Source: RGD

mitochondrion

Inferred from electronic annotation. Source: Ensembl

perinuclear region of cytoplasm

Inferred from direct assay Ref.2. Source: RGD

peroxisomal membrane

Inferred from electronic annotation. Source: Ensembl

phagocytic vesicle

Inferred from sequence or structural similarity. Source: UniProtKB

phagocytic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from direct assay Ref.1. Source: RGD

   Molecular_functionGDP binding

Inferred from sequence or structural similarity. Source: UniProtKB

GTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

TPR domain binding

Inferred from physical interaction Ref.2. Source: RGD

protein binding

Inferred from physical interaction Ref.3. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 204204Ras-related protein Rab-8B
PRO_0000121136
Propeptide205 – 2073Removed in mature form Potential
PRO_0000370803

Regions

Nucleotide binding15 – 228GTP By similarity
Nucleotide binding63 – 675GTP By similarity
Nucleotide binding121 – 1244GTP By similarity
Motif37 – 459Effector region By similarity

Amino acid modifications

Modified residue1801Phosphoserine By similarity
Modified residue1831Phosphoserine By similarity
Modified residue2041Cysteine methyl ester Potential
Lipidation2041S-geranylgeranyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
P70550 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 4A41AB26BF9DCAF4

FASTA20723,603
        10         20         30         40         50         60 
MAKTYDYLFK LLLIGDSGVG KTCLLFRFSE DAFNTTFIST IGIDFKIRTI ELDGKKIKLQ 

        70         80         90        100        110        120 
IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIK NWIRNIEEHA SSDVERMILG 

       130        140        150        160        170        180 
NKCDMNDKRQ VSKERGEKLA IDYGIKFLET SAKSSTNVEE AFFTLARDIM TKLNRKMNDS 

       190        200 
NSSGAGGPVK ITESRSKKTS FFRCSLL 

« Hide

References

[1]"Identification of a novel member of the Rab8 family from the rat basophilic leukaemia cell line, RBL.2H3."
Armstrong J., Thompson N., Squire J.H., Smith J., Hayes B., Solari R.
J. Cell Sci. 109:1265-1274(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Rab8b and its interacting partner TRIP8b are involved in regulated secretion in AtT20 cells."
Chen S., Liang M.C., Chia J.N., Ngsee J.K., Ting A.E.
J. Biol. Chem. 276:13209-13216(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PEX5R.
[3]"Rab8B GTPase and junction dynamics in the testis."
Lau A.S., Mruk D.D.
Endocrinology 144:1549-1563(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN CELL JUNCTION DYNAMICS, ASSOCIATION WITH ACTIN; DELTA-CATENIN AND ALPHA AND BETA TUBULINS, INTERACTION WITH VIM AND CDH1, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U53475 mRNA. Translation: AAA99782.1.
RefSeqNP_695229.1. NM_153317.1.
UniGeneRn.10995.

3D structure databases

ProteinModelPortalP70550.
SMRP70550. Positions 6-176.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid251776. 1 interaction.
IntActP70550. 1 interaction.
MINTMINT-4566455.

PTM databases

PhosphoSiteP70550.

Proteomic databases

PaxDbP70550.
PRIDEP70550.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000024286; ENSRNOP00000024287; ENSRNOG00000018009.
GeneID266688.
KEGGrno:266688.
UCSCRGD:628764. rat.

Organism-specific databases

CTD51762.
RGD628764. Rab8b.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00750000117584.
HOGENOMHOG000233968.
HOVERGENHBG009351.
InParanoidP70550.
KOK07902.
OMATWHANID.
OrthoDBEOG7VB2H4.
PhylomeDBP70550.
TreeFamTF314097.

Gene expression databases

GenevestigatorP70550.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio624500.
PROP70550.

Entry information

Entry nameRAB8B_RAT
AccessionPrimary (citable) accession number: P70550
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families