P70550 (RAB8B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-8B

Gene names

Name:

Rab8b

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in vesicular trafficking and neurotransmitter release By similarity. May participate in cell junction dynamics in Sertoli cells. Ref.3
Subunit structure	Interacts with OTOF, PEX5R/TRIP8B and RAB3IP By similarity. Interacts with VIM AND CDH1. Associated with actin, delta-catenin and alpha and beta tubulins. Ref.2 Ref.3
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Cytoplasmic vesicle › phagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Recruited to phagosomes containing S.aureus or Mycobacterium By similarity. Colocalizes with CDH1 in the basal compartment.
Tissue specificity	Hight levels of expression in the spleen, heart, kidney, testis and brain. Expression increases in Sertoli and germ cells during their maturation. Ref.3
Developmental stage	Localizes in the basal compartment associating with spermatocytes in all stages of the spermatogenic cycle. Detected at the site of elongate spermatids in stages XII-XIV in addition to being found in the basal compartment in these stages. Ref.3
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adherens junction organization Inferred from direct assay Ref.3. Source: UniProtKB positive regulation of cell projection organization Inferred from direct assay Ref.2. Source: RGD positive regulation of corticotropin secretion Inferred from direct assay Ref.2. Source: RGD protein import into peroxisome membrane Inferred from electronic annotation. Source: Compara small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cell tip Inferred from direct assay Ref.2. Source: RGD mitochondrion Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from direct assay Ref.2. Source: RGD peroxisomal membrane Inferred from electronic annotation. Source: Compara phagocytic vesicle Inferred from sequence or structural similarity. Source: UniProtKB phagocytic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay Ref.1. Source: RGD
Molecular_function	GDP binding Inferred from sequence or structural similarity. Source: UniProtKB GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	Ras-related protein Rab-8B		PRO_0000121136
	Propeptide	205 – 207	3	Removed in mature form Potential		PRO_0000370803
Regions
	Nucleotide binding	15 – 22	8	GTP By similarity
	Nucleotide binding	63 – 67	5	GTP By similarity
	Nucleotide binding	121 – 124	4	GTP By similarity
	Motif	37 – 45	9	Effector region By similarity
Amino acid modifications
	Modified residue	204	1	Cysteine methyl ester Potential
	Lipidation	204	1	S-geranylgeranyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70550 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 4A41AB26BF9DCAF4	FASTA	207	23,603
	10 20 30 40 50 60 MAKTYDYLFK LLLIGDSGVG KTCLLFRFSE DAFNTTFIST IGIDFKIRTI ELDGKKIKLQ 70 80 90 100 110 120 IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIK NWIRNIEEHA SSDVERMILG 130 140 150 160 170 180 NKCDMNDKRQ VSKERGEKLA IDYGIKFLET SAKSSTNVEE AFFTLARDIM TKLNRKMNDS 190 200 NSSGAGGPVK ITESRSKKTS FFRCSLL

References

[1]	"Identification of a novel member of the Rab8 family from the rat basophilic leukaemia cell line, RBL.2H3." Armstrong J., Thompson N., Squire J.H., Smith J., Hayes B., Solari R. J. Cell Sci. 109:1265-1274(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Rab8b and its interacting partner TRIP8b are involved in regulated secretion in AtT20 cells." Chen S., Liang M.C., Chia J.N., Ngsee J.K., Ting A.E. J. Biol. Chem. 276:13209-13216(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PEX5R.
[3]	"Rab8B GTPase and junction dynamics in the testis." Lau A.S., Mruk D.D. Endocrinology 144:1549-1563(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, FUNCTION IN CELL JUNCTION DYNAMICS, ASSOCIATION WITH ACTIN; DELTA-CATENIN AND ALPHA AND BETA TUBULINS, INTERACTION WITH VIM AND CDH1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U53475 mRNA. Translation: AAA99782.1.
IPI	IPI00189690.
RefSeq	NP_695229.1. NM_153317.1.
UniGene	Rn.10995.
3D structure databases
ProteinModelPortal	P70550.
SMR	P70550. Positions 6-176.
ModBase	Search...
Protein-protein interaction databases
IntAct	P70550. 1 interaction.
PTM databases
PhosphoSite	P70550.
Proteomic databases
PaxDb	P70550.
PRIDE	P70550.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000024286; ENSRNOP00000024287; ENSRNOG00000018009.
GeneID	266688.
KEGG	rno:266688.
UCSC	RGD:628764. rat.
Organism-specific databases
CTD	51762.
RGD	628764. Rab8b.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00700000104031.
HOGENOM	HOG000233968.
HOVERGEN	HBG009351.
InParanoid	P70550.
KO	K07902.
OMA	TSFFRCL.
Gene expression databases
ArrayExpress	P70550.
Genevestigator	P70550.
GermOnline	ENSRNOG00000018009. Rattus norvegicus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	624500.

Entry information

Entry name

RAB8B_RAT

Accession

Primary (citable) accession number: P70550

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program