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Protein

Ras-related protein Rab-8B

Gene

Rab8b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may be involved in polarized vesicular trafficking and neurotransmitter release. May participate in cell junction dynamics in Sertoli cells.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTPBy similarity8
Nucleotide bindingi63 – 67GTPBy similarity5
Nucleotide bindingi121 – 124GTPBy similarity4

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • receptor binding Source: RGD
  • TPR domain binding Source: RGD

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-8854214. TBC/RABGAPs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-8B
Gene namesi
Name:Rab8b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi628764. Rab8b.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001211361 – 204Ras-related protein Rab-8BAdd BLAST204
PropeptideiPRO_0000370803205 – 207Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei180PhosphoserineBy similarity1
Modified residuei183PhosphoserineBy similarity1
Modified residuei204Cysteine methyl esterSequence analysis1
Lipidationi204S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP70550.
PRIDEiP70550.

PTM databases

iPTMnetiP70550.
PhosphoSitePlusiP70550.

Expressioni

Tissue specificityi

Hight levels of expression in the spleen, heart, kidney, testis and brain. Expression increases in Sertoli and germ cells during their maturation.1 Publication

Developmental stagei

Localizes in the basal compartment associating with spermatocytes in all stages of the spermatogenic cycle. Detected at the site of elongate spermatids in stages XII-XIV in addition to being found in the basal compartment in these stages.1 Publication

Gene expression databases

BgeeiENSRNOG00000018009.
ExpressionAtlasiP70550. baseline and differential.
GenevisibleiP70550. RN.

Interactioni

Subunit structurei

Associated with actin, delta-catenin and alpha and beta tubulins. Interacts with OTOF (By similarity). Interacts with PEX5R. Interacts with RAB3IP (By similarity). Interacts with VIM. Interacts with CDH1. Interacts with MICALL2 (By similarity).By similarity

GO - Molecular functioni

  • receptor binding Source: RGD
  • TPR domain binding Source: RGD

Protein-protein interaction databases

BioGridi251776. 1 interactor.
IntActiP70550. 1 interactor.
MINTiMINT-4566455.
STRINGi10116.ENSRNOP00000024287.

Structurei

3D structure databases

ProteinModelPortaliP70550.
SMRiP70550.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0078. Eukaryota.
ENOG410XPUI. LUCA.
GeneTreeiENSGT00890000139330.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP70550.
KOiK07902.
OMAiNRKMNDG.
OrthoDBiEOG091G0LA6.
PhylomeDBiP70550.
TreeFamiTF314097.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51419. RAB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70550-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTYDYLFK LLLIGDSGVG KTCLLFRFSE DAFNTTFIST IGIDFKIRTI
60 70 80 90 100
ELDGKKIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIK
110 120 130 140 150
NWIRNIEEHA SSDVERMILG NKCDMNDKRQ VSKERGEKLA IDYGIKFLET
160 170 180 190 200
SAKSSTNVEE AFFTLARDIM TKLNRKMNDS NSSGAGGPVK ITESRSKKTS

FFRCSLL
Length:207
Mass (Da):23,603
Last modified:February 1, 1997 - v1
Checksum:i4A41AB26BF9DCAF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53475 mRNA. Translation: AAA99782.1.
RefSeqiNP_695229.1. NM_153317.1.
UniGeneiRn.10995.

Genome annotation databases

EnsembliENSRNOT00000024286; ENSRNOP00000024287; ENSRNOG00000018009.
GeneIDi266688.
KEGGirno:266688.
UCSCiRGD:628764. rat.

Similar proteinsi

Entry informationi

Entry nameiRAB8B_RAT
AccessioniPrimary (citable) accession number: P70550
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 22, 2017
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families