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Protein

Ras-related protein Rab-8B

Gene

Rab8b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may be involved in polarized vesicular trafficking and neurotransmitter release. May participate in cell junction dynamics in Sertoli cells.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228GTPBy similarity
Nucleotide bindingi63 – 675GTPBy similarity
Nucleotide bindingi121 – 1244GTPBy similarity

GO - Molecular functioni

  1. GDP binding Source: UniProtKB
  2. GTPase activity Source: GO_Central
  3. GTP binding Source: UniProtKB-KW
  4. TPR domain binding Source: RGD

GO - Biological processi

  1. adherens junction organization Source: UniProtKB
  2. antigen processing and presentation Source: Ensembl
  3. cellular response to insulin stimulus Source: GO_Central
  4. Golgi vesicle fusion to target membrane Source: GO_Central
  5. intracellular protein transport Source: GO_Central
  6. metabolic process Source: GOC
  7. positive regulation of cell projection organization Source: RGD
  8. positive regulation of corticotropin secretion Source: RGD
  9. protein import into peroxisome membrane Source: Ensembl
  10. protein localization to plasma membrane Source: GO_Central
  11. protein secretion Source: GO_Central
  12. Rab protein signal transduction Source: GO_Central
  13. regulation of exocytosis Source: GO_Central
  14. synaptic vesicle exocytosis Source: GO_Central
  15. vesicle docking involved in exocytosis Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-8B
Gene namesi
Name:Rab8b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi628764. Rab8b.

Subcellular locationi

  1. Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated
  2. Cytoplasmic vesiclephagosome membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity

  3. Note: Recruited to phagosomes containing S.aureus or Mycobacterium (By similarity). Colocalizes with CDH1 in the basal compartment.By similarity

GO - Cellular componenti

  1. cell tip Source: RGD
  2. cytoplasmic vesicle Source: RGD
  3. endosome Source: GO_Central
  4. extracellular vesicular exosome Source: Ensembl
  5. mitochondrion Source: Ensembl
  6. perinuclear region of cytoplasm Source: RGD
  7. peroxisomal membrane Source: Ensembl
  8. phagocytic vesicle Source: UniProtKB
  9. phagocytic vesicle membrane Source: UniProtKB-SubCell
  10. plasma membrane Source: RGD
  11. secretory granule membrane Source: GO_Central
  12. synaptic vesicle Source: GO_Central
  13. trans-Golgi network transport vesicle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Ras-related protein Rab-8BPRO_0000121136Add
BLAST
Propeptidei205 – 2073Removed in mature formSequence AnalysisPRO_0000370803

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei180 – 1801PhosphoserineBy similarity
Modified residuei183 – 1831PhosphoserineBy similarity
Modified residuei204 – 2041Cysteine methyl esterSequence Analysis
Lipidationi204 – 2041S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP70550.
PRIDEiP70550.

PTM databases

PhosphoSiteiP70550.

Expressioni

Tissue specificityi

Hight levels of expression in the spleen, heart, kidney, testis and brain. Expression increases in Sertoli and germ cells during their maturation.1 Publication

Developmental stagei

Localizes in the basal compartment associating with spermatocytes in all stages of the spermatogenic cycle. Detected at the site of elongate spermatids in stages XII-XIV in addition to being found in the basal compartment in these stages.1 Publication

Gene expression databases

ExpressionAtlasiP70550. baseline and differential.
GenevestigatoriP70550.

Interactioni

Subunit structurei

Associated with actin, delta-catenin and alpha and beta tubulins. Interacts with OTOF (By similarity). Interacts with PEX5R. Interacts with RAB3IP (By similarity). Interacts with VIM. Interacts with CDH1. Interacts with MICALL2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi251776. 1 interaction.
IntActiP70550. 1 interaction.
MINTiMINT-4566455.

Structurei

3D structure databases

ProteinModelPortaliP70550.
SMRiP70550. Positions 6-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 459Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118937.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP70550.
KOiK07902.
OMAiGNKSDWS.
OrthoDBiEOG7VB2H4.
PhylomeDBiP70550.
TreeFamiTF314097.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70550-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTYDYLFK LLLIGDSGVG KTCLLFRFSE DAFNTTFIST IGIDFKIRTI
60 70 80 90 100
ELDGKKIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIK
110 120 130 140 150
NWIRNIEEHA SSDVERMILG NKCDMNDKRQ VSKERGEKLA IDYGIKFLET
160 170 180 190 200
SAKSSTNVEE AFFTLARDIM TKLNRKMNDS NSSGAGGPVK ITESRSKKTS

FFRCSLL
Length:207
Mass (Da):23,603
Last modified:February 1, 1997 - v1
Checksum:i4A41AB26BF9DCAF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53475 mRNA. Translation: AAA99782.1.
RefSeqiNP_695229.1. NM_153317.1.
UniGeneiRn.10995.

Genome annotation databases

EnsembliENSRNOT00000024286; ENSRNOP00000024287; ENSRNOG00000018009.
GeneIDi266688.
KEGGirno:266688.
UCSCiRGD:628764. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53475 mRNA. Translation: AAA99782.1.
RefSeqiNP_695229.1. NM_153317.1.
UniGeneiRn.10995.

3D structure databases

ProteinModelPortaliP70550.
SMRiP70550. Positions 6-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251776. 1 interaction.
IntActiP70550. 1 interaction.
MINTiMINT-4566455.

PTM databases

PhosphoSiteiP70550.

Proteomic databases

PaxDbiP70550.
PRIDEiP70550.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024286; ENSRNOP00000024287; ENSRNOG00000018009.
GeneIDi266688.
KEGGirno:266688.
UCSCiRGD:628764. rat.

Organism-specific databases

CTDi51762.
RGDi628764. Rab8b.

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118937.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP70550.
KOiK07902.
OMAiGNKSDWS.
OrthoDBiEOG7VB2H4.
PhylomeDBiP70550.
TreeFamiTF314097.

Miscellaneous databases

NextBioi624500.
PROiP70550.

Gene expression databases

ExpressionAtlasiP70550. baseline and differential.
GenevestigatoriP70550.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of a novel member of the Rab8 family from the rat basophilic leukaemia cell line, RBL.2H3."
    Armstrong J., Thompson N., Squire J.H., Smith J., Hayes B., Solari R.
    J. Cell Sci. 109:1265-1274(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Rab8b and its interacting partner TRIP8b are involved in regulated secretion in AtT20 cells."
    Chen S., Liang M.C., Chia J.N., Ngsee J.K., Ting A.E.
    J. Biol. Chem. 276:13209-13216(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PEX5R.
  3. "Rab8B GTPase and junction dynamics in the testis."
    Lau A.S., Mruk D.D.
    Endocrinology 144:1549-1563(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CELL JUNCTION DYNAMICS, ASSOCIATION WITH ACTIN; DELTA-CATENIN AND ALPHA AND BETA TUBULINS, INTERACTION WITH VIM AND CDH1, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiRAB8B_RAT
AccessioniPrimary (citable) accession number: P70550
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 29, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.