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P70550

- RAB8B_RAT

UniProt

P70550 - RAB8B_RAT

Protein

Ras-related protein Rab-8B

Gene

Rab8b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may be involved in polarized vesicular trafficking and neurotransmitter release. May participate in cell junction dynamics in Sertoli cells.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 228GTPBy similarity
    Nucleotide bindingi63 – 675GTPBy similarity
    Nucleotide bindingi121 – 1244GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: UniProtKB
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. TPR domain binding Source: RGD

    GO - Biological processi

    1. adherens junction organization Source: UniProtKB
    2. positive regulation of cell projection organization Source: RGD
    3. positive regulation of corticotropin secretion Source: RGD
    4. protein import into peroxisome membrane Source: Ensembl
    5. small GTPase mediated signal transduction Source: InterPro

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-8B
    Gene namesi
    Name:Rab8b
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi628764. Rab8b.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated. Cytoplasmic vesiclephagosome membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
    Note: Recruited to phagosomes containing S.aureus or Mycobacterium By similarity. Colocalizes with CDH1 in the basal compartment.By similarity

    GO - Cellular componenti

    1. cell tip Source: RGD
    2. cytoplasmic vesicle Source: RGD
    3. mitochondrion Source: Ensembl
    4. perinuclear region of cytoplasm Source: RGD
    5. peroxisomal membrane Source: Ensembl
    6. phagocytic vesicle Source: UniProtKB
    7. phagocytic vesicle membrane Source: UniProtKB-SubCell
    8. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 204204Ras-related protein Rab-8BPRO_0000121136Add
    BLAST
    Propeptidei205 – 2073Removed in mature formSequence AnalysisPRO_0000370803

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei180 – 1801PhosphoserineBy similarity
    Modified residuei183 – 1831PhosphoserineBy similarity
    Modified residuei204 – 2041Cysteine methyl esterSequence Analysis
    Lipidationi204 – 2041S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Proteomic databases

    PaxDbiP70550.
    PRIDEiP70550.

    PTM databases

    PhosphoSiteiP70550.

    Expressioni

    Tissue specificityi

    Hight levels of expression in the spleen, heart, kidney, testis and brain. Expression increases in Sertoli and germ cells during their maturation.1 Publication

    Developmental stagei

    Localizes in the basal compartment associating with spermatocytes in all stages of the spermatogenic cycle. Detected at the site of elongate spermatids in stages XII-XIV in addition to being found in the basal compartment in these stages.1 Publication

    Gene expression databases

    GenevestigatoriP70550.

    Interactioni

    Subunit structurei

    Associated with actin, delta-catenin and alpha and beta tubulins. Interacts with OTOF By similarity. Interacts with PEX5R. Interacts with RAB3IP By similarity. Interacts with VIM. Interacts with CDH1. Interacts with MICALL2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi251776. 1 interaction.
    IntActiP70550. 1 interaction.
    MINTiMINT-4566455.

    Structurei

    3D structure databases

    ProteinModelPortaliP70550.
    SMRiP70550. Positions 6-176.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi37 – 459Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00750000117584.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiP70550.
    KOiK07902.
    OMAiTWHANID.
    OrthoDBiEOG7VB2H4.
    PhylomeDBiP70550.
    TreeFamiTF314097.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P70550-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKTYDYLFK LLLIGDSGVG KTCLLFRFSE DAFNTTFIST IGIDFKIRTI    50
    ELDGKKIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIK 100
    NWIRNIEEHA SSDVERMILG NKCDMNDKRQ VSKERGEKLA IDYGIKFLET 150
    SAKSSTNVEE AFFTLARDIM TKLNRKMNDS NSSGAGGPVK ITESRSKKTS 200
    FFRCSLL 207
    Length:207
    Mass (Da):23,603
    Last modified:February 1, 1997 - v1
    Checksum:i4A41AB26BF9DCAF4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53475 mRNA. Translation: AAA99782.1.
    RefSeqiNP_695229.1. NM_153317.1.
    UniGeneiRn.10995.

    Genome annotation databases

    EnsembliENSRNOT00000024286; ENSRNOP00000024287; ENSRNOG00000018009.
    GeneIDi266688.
    KEGGirno:266688.
    UCSCiRGD:628764. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53475 mRNA. Translation: AAA99782.1 .
    RefSeqi NP_695229.1. NM_153317.1.
    UniGenei Rn.10995.

    3D structure databases

    ProteinModelPortali P70550.
    SMRi P70550. Positions 6-176.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 251776. 1 interaction.
    IntActi P70550. 1 interaction.
    MINTi MINT-4566455.

    PTM databases

    PhosphoSitei P70550.

    Proteomic databases

    PaxDbi P70550.
    PRIDEi P70550.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000024286 ; ENSRNOP00000024287 ; ENSRNOG00000018009 .
    GeneIDi 266688.
    KEGGi rno:266688.
    UCSCi RGD:628764. rat.

    Organism-specific databases

    CTDi 51762.
    RGDi 628764. Rab8b.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00750000117584.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi P70550.
    KOi K07902.
    OMAi TWHANID.
    OrthoDBi EOG7VB2H4.
    PhylomeDBi P70550.
    TreeFami TF314097.

    Miscellaneous databases

    NextBioi 624500.
    PROi P70550.

    Gene expression databases

    Genevestigatori P70550.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a novel member of the Rab8 family from the rat basophilic leukaemia cell line, RBL.2H3."
      Armstrong J., Thompson N., Squire J.H., Smith J., Hayes B., Solari R.
      J. Cell Sci. 109:1265-1274(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Rab8b and its interacting partner TRIP8b are involved in regulated secretion in AtT20 cells."
      Chen S., Liang M.C., Chia J.N., Ngsee J.K., Ting A.E.
      J. Biol. Chem. 276:13209-13216(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PEX5R.
    3. "Rab8B GTPase and junction dynamics in the testis."
      Lau A.S., Mruk D.D.
      Endocrinology 144:1549-1563(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN CELL JUNCTION DYNAMICS, ASSOCIATION WITH ACTIN; DELTA-CATENIN AND ALPHA AND BETA TUBULINS, INTERACTION WITH VIM AND CDH1, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiRAB8B_RAT
    AccessioniPrimary (citable) accession number: P70550
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3