Reviewed, UniProtKB/Swiss-Prot P70531 (EF2K_RAT)

Names and origin

Protein names

Recommended name:
    Elongation factor 2 kinase
    EC=2.7.11.20
Alternative name(s):
    eEF-2 kinase
      Short name=eEF-2K
    Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase

Gene names

Name:

Eef2k

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	In vitro, it phosphorylates 2 adjacent threonine residues, 'Thr-57' and 'Thr-59', in the N-terminus of eukaryotic elongation factor-2. Binds calmodulin.
Catalytic activity	ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.
Enzyme regulation	Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.
Subunit structure	Monomer or homodimer Potential.
Tissue specificity	Mostly in skeletal muscle.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the protein kinase superfamily. Alpha-type protein kinase family. Contains 1 alpha-type protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Calcium Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW elongation factor-2 kinase activity Ref.1 Inferred from mutant phenotype. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Elongation factor 2 kinase		PRO_0000086938
Regions
	Domain	115 – 325	211	Alpha-type protein kinase
	Nucleotide binding	295 – 301	7	ATP By similarity
	Region	593 – 609	17	Calmodulin-binding Potential
	Region	609 – 626	18	Pseudosubstrate/autoinhibitory domain Potential
Amino acid modifications
	Modified residue	18	1	Phosphoserine By similarity
	Modified residue	27	1	Phosphoserine By similarity
	Modified residue	29	1	Phosphothreonine By similarity
	Modified residue	31	1	Phosphoserine By similarity
	Modified residue	70	1	Phosphoserine By similarity
	Modified residue	134	1	Phosphoserine By similarity
	Modified residue	347	1	Phosphothreonine By similarity
	Modified residue	444	1	Phosphoserine By similarity
	Modified residue	469	1	Phosphoserine By similarity
	Modified residue	473	1	Phosphoserine By similarity
	Modified residue	476	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70531-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E4DDD832AE9F7165	FASTA	724	81,489
	10 20 30 40 50 60 MADEDLIFRL EGVDGGGSSG AGRHGDSDTD SDDDEGYFIC PITDDHMSNQ NVNSKGQGYY 70 80 90 100 110 120 NNLLKTECGS TGSPASSFHF KEAWKHAIEK AKHMPDPWAE FHLEDIATEH ATRHRYNAVT 130 140 150 160 170 180 GEWLKDEVLI KMASQPFGRG AMRECFRTKK LSNFLHAQHW KGASNYVAKR YLEPVDRSVY 190 200 210 220 230 240 FEDVQLQMEA KLWGEEYNRH KPPKQVDIMQ MCIIELKDRQ GQPLFHLEHY IEGKYIKYNS 250 260 270 280 290 300 NSGFVRDDNI RLTPQAFSHF TFERSGHQLI VVDIQGVGDL YTDPQIHTEK GTDFGDGNLG 310 320 330 340 350 360 VRGMALFFYS HACNRICQSM GLAPFDLSPR EQDAVNQSTK LLQSAKTILR GTEEKCGSPR 370 380 390 400 410 420 IRTLSGSRPP LLLRLSENSG DENMSDVTFD SLPSSPSSAT PHSQKLDHLH WPVFGDLDNM 430 440 450 460 470 480 GPRDHDRMDN HRDSENSGDS GYPSEKRSDL DDPEPREHGH SNGNRRPESD EDSLGSSGRV 490 500 510 520 530 540 CVETWNLLNP SRLHLPRPSA VALEVQRLNA LDLGRKIGKS VLGKVHLAMV RYHEGGRFCE 550 560 570 580 590 600 KDEEWDQESA IFHLEHAADL GELEAIVGLG LMYSQLPHHI LADVSLEETE ENKTKGFDYL 610 620 630 640 650 660 LKAAEAGDRQ SMILVARAFD TGLNLSPDRC QDWSEALHWY NTALETTDCD EGGEYDGIQD 670 680 690 700 710 720 EPQYALLARE AEMLLTGGFG LDKNPQRSGD LYTQAAEAAM EAMKGRLANQ YYEKAEEAWA QMEE

References

[1]	"Cloning and expression of cDNA encoding protein synthesis elongation factor-2 kinase." Redpath N.T., Price N.T., Proud C.G. J. Biol. Chem. 271:17547-17554(1996) [PubMed: 8663182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[2]	"Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase." Ryazanov A.G., Ward M.D., Mendola C.E., Pavur K.S., Dorovkov M.V., Wiedmann M., Erdjument-Bromage H., Tempst P., Parmer T.G., Prostko C.R., Germino F.J., Hait W.N. Proc. Natl. Acad. Sci. U.S.A. 94:4884-4889(1997) [PubMed: 9144159] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New England Deaconess Hospital. Tissue: Pheochromocytoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X96426 mRNA. Translation: CAA65286.1. U93849 mRNA. Translation: AAB58272.1.
IPI	IPI00189647.
RefSeq	NP_037079.1.
UniGene	Rn.10958
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P70531.
PTM databases
PhosphoSite	P70531.
Genome annotation databases
Ensembl	ENSRNOT00000022726; ENSRNOP00000022726; ENSRNOG00000016448; Rattus norvegicus. [Genome view]
GeneID	25435.
KEGG	rno:25435.
UCSC	BC061825. rat.
Organism-specific databases
CTD	25435.
RGD	2538. Eef2k.
Phylogenomic databases
HOVERGEN	P70531.
OMA	PKQVDIM.
Enzyme and pathway databases
BRENDA	2.7.11.20. 248.
Gene expression databases
ArrayExpress	P70531.
Genevestigator	P70531.
GermOnline	ENSRNOG00000016448. Rattus norvegicus.
Family and domain databases
InterPro	IPR017400. Elongation_factor_2_kinase. IPR004166. MHCK_EF2_kinase. IPR006597. Sel1-like. IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
Pfam	PF02816. Alpha_kinase. 1 hit. PF08238. Sel1. 3 hits. [Graphical view]
PIRSF	PIRSF038139. Elongation_factor_2_kinase. 1 hit.
SMART	SM00811. Alpha_kinase. 1 hit. [Graphical view]
PROSITE	PS51158. ALPHA_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	606641.

Entry information

Entry name

EF2K_RAT

Accession

Primary (citable) accession number: P70531
Secondary accession number(s): O09089

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)