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Protein

Hepatocyte nuclear factor 6

Gene

Onecut1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. The affinity of HNF-6-alpha and HNF-6-beta for DNA differs depending on the target sequence.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi283 – 369CUTPROSITE-ProRule annotationAdd BLAST87
DNA bindingi385 – 444HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • DNA binding Source: RGD
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: BHF-UCL
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: RGD

GO - Biological processi

  • cell differentiation Source: GO_Central
  • glucose metabolic process Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • system development Source: GO_Central

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte nuclear factor 6
Short name:
HNF-6
Alternative name(s):
One cut domain family member 1
One cut homeobox 1
Gene namesi
Name:Onecut1
Synonyms:Hnf6, Hnf6a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2811. Onecut1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002024041 – 465Hepatocyte nuclear factor 6Add BLAST465

Expressioni

Tissue specificityi

Expressed in liver, brain, spleen and testis.

Interactioni

Subunit structurei

Binds DNA as a monomer.

Structurei

Secondary structure

1465
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi294 – 307Combined sources14
Helixi312 – 318Combined sources7
Helixi324 – 332Combined sources9
Helixi337 – 339Combined sources3
Helixi344 – 355Combined sources12
Helixi358 – 364Combined sources7
Helixi394 – 406Combined sources13
Helixi412 – 422Combined sources11
Helixi426 – 438Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D5VX-ray2.00A/B289-444[»]
ProteinModelPortaliP70512.
SMRiP70512.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70512.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi69 – 72Poly-His4
Compositional biasi124 – 138Poly-HisAdd BLAST15
Compositional biasi455 – 460Poly-Ser6

Sequence similaritiesi

Belongs to the CUT homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

HOGENOMiHOG000232114.
HOVERGENiHBG005981.
InParanoidiP70512.
KOiK08026.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiView protein in InterPro
IPR003350. CUT_dom.
IPR009057. Homeobox-like.
IPR001356. Homeobox_dom.
IPR010982. Lambda_DNA-bd_dom.
PfamiView protein in Pfam
PF02376. CUT. 1 hit.
PF00046. Homeobox. 1 hit.
SMARTiView protein in SMART
SM01109. CUT. 1 hit.
SM00389. HOX. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiView protein in PROSITE
PS51042. CUT. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: P70512-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNAQLTMEAI GELHGVSHEP VPAPADLLGG SPHARSSVGH RGSHLPPAHP
60 70 80 90 100
RSMGMASLLD GGSGGSDYHH HHRAPEHSLA GPLHPTMTMA CETPPGMSMP
110 120 130 140 150
TTYTTLTPLQ PLPPISTVSD KFPHHHHHHH HHHHPHHHQR LAGNVSGSFT
160 170 180 190 200
LMRDERGLAS MNNLYTPYHK DVAGMGQSLS PLSGSGLGSI HNSQQGLPHY
210 220 230 240 250
AHPGAAMPTD KMLTPNDFEA HHPAMLGRHG EQHLTPTSAG MVPINGLPPH
260 270 280 290 300
HPHAHLNAQG HGQLLGTARE PNPSVTGAQV SNGSNSGQME EINTKEVAQR
310 320 330 340 350
ITTELKRYSI PQAIFAQRVL CRSQGTLSDL LRNPKPWSKL KSGRETFRRM
360 370 380 390 400
WKWLQEPEFQ RMSALRLAAC KRKEQEHGKD RGNTPKKPRL VFTDVQRRTL
410 420 430 440 450
HAIFKENKRP SKELQITISQ QLGLELSTVS NFFMNARRRS LDKWQDEGSS
460
NSGNSSSSSS TCTKA
Length:465
Mass (Da):51,067
Last modified:February 1, 1997 - v1
Checksum:iBD651267FD7AC896
GO
Isoform Beta (identifier: P70512-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     368-368: A → AESAMGGSVPSLRITSGGPQLSVPPLP

Show »
Length:491
Mass (Da):53,584
Checksum:iC0B87B4FB6F6C978
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002312368A → AESAMGGSVPSLRITSGGPQ LSVPPLP in isoform Beta. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96553 Genomic DNA. Translation: CAA65389.1.
Y14933 mRNA. Translation: CAA75150.1.
RefSeqiNP_073162.1. NM_022671.2. [P70512-1]
UniGeneiRn.48812.

Genome annotation databases

GeneIDi25231.
KEGGirno:25231.
UCSCiRGD:2811. rat. [P70512-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96553 Genomic DNA. Translation: CAA65389.1.
Y14933 mRNA. Translation: CAA75150.1.
RefSeqiNP_073162.1. NM_022671.2. [P70512-1]
UniGeneiRn.48812.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D5VX-ray2.00A/B289-444[»]
ProteinModelPortaliP70512.
SMRiP70512.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25231.
KEGGirno:25231.
UCSCiRGD:2811. rat. [P70512-1]

Organism-specific databases

CTDi3175.
RGDi2811. Onecut1.

Phylogenomic databases

HOGENOMiHOG000232114.
HOVERGENiHBG005981.
InParanoidiP70512.
KOiK08026.

Miscellaneous databases

EvolutionaryTraceiP70512.
PROiP70512.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiView protein in InterPro
IPR003350. CUT_dom.
IPR009057. Homeobox-like.
IPR001356. Homeobox_dom.
IPR010982. Lambda_DNA-bd_dom.
PfamiView protein in Pfam
PF02376. CUT. 1 hit.
PF00046. Homeobox. 1 hit.
SMARTiView protein in SMART
SM01109. CUT. 1 hit.
SM00389. HOX. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiView protein in PROSITE
PS51042. CUT. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHNF6_RAT
AccessioniPrimary (citable) accession number: P70512
Secondary accession number(s): O88755
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: February 15, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.