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Protein

Hepatocyte nuclear factor 6

Gene

Onecut1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. The affinity of HNF-6-alpha and HNF-6-beta for DNA differs depending on the target sequence.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi283 – 36987CUTPROSITE-ProRule annotationAdd
BLAST
DNA bindingi385 – 44460HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: RGD
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: BHF-UCL
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: RGD

GO - Biological processi

  • glucose metabolic process Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte nuclear factor 6
Short name:
HNF-6
Alternative name(s):
One cut domain family member 1
One cut homeobox 1
Gene namesi
Name:Onecut1
Synonyms:Hnf6, Hnf6a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2811. Onecut1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465Hepatocyte nuclear factor 6PRO_0000202404Add
BLAST

Expressioni

Tissue specificityi

Expressed in liver, brain, spleen and testis.

Interactioni

Subunit structurei

Binds DNA as a monomer.

Structurei

Secondary structure

1
465
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi294 – 30714
Helixi312 – 3187
Helixi324 – 3329
Helixi337 – 3393
Helixi344 – 35512
Helixi358 – 3647
Helixi394 – 40613
Helixi412 – 42211
Helixi426 – 43813

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D5VX-ray2.00A/B289-444[»]
ProteinModelPortaliP70512.
SMRiP70512. Positions 291-437.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70512.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi69 – 724Poly-His
Compositional biasi124 – 13815Poly-HisAdd
BLAST
Compositional biasi455 – 4606Poly-Ser

Sequence similaritiesi

Belongs to the CUT homeobox family.Curated
Contains 1 CUT DNA-binding domain.PROSITE-ProRule annotation
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

HOGENOMiHOG000232114.
HOVERGENiHBG005981.
InParanoidiP70512.
KOiK08026.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR003350. CUT_dom.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF02376. CUT. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM01109. CUT. 1 hit.
SM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS51042. CUT. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: P70512-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNAQLTMEAI GELHGVSHEP VPAPADLLGG SPHARSSVGH RGSHLPPAHP
60 70 80 90 100
RSMGMASLLD GGSGGSDYHH HHRAPEHSLA GPLHPTMTMA CETPPGMSMP
110 120 130 140 150
TTYTTLTPLQ PLPPISTVSD KFPHHHHHHH HHHHPHHHQR LAGNVSGSFT
160 170 180 190 200
LMRDERGLAS MNNLYTPYHK DVAGMGQSLS PLSGSGLGSI HNSQQGLPHY
210 220 230 240 250
AHPGAAMPTD KMLTPNDFEA HHPAMLGRHG EQHLTPTSAG MVPINGLPPH
260 270 280 290 300
HPHAHLNAQG HGQLLGTARE PNPSVTGAQV SNGSNSGQME EINTKEVAQR
310 320 330 340 350
ITTELKRYSI PQAIFAQRVL CRSQGTLSDL LRNPKPWSKL KSGRETFRRM
360 370 380 390 400
WKWLQEPEFQ RMSALRLAAC KRKEQEHGKD RGNTPKKPRL VFTDVQRRTL
410 420 430 440 450
HAIFKENKRP SKELQITISQ QLGLELSTVS NFFMNARRRS LDKWQDEGSS
460
NSGNSSSSSS TCTKA
Length:465
Mass (Da):51,067
Last modified:February 1, 1997 - v1
Checksum:iBD651267FD7AC896
GO
Isoform Beta (identifier: P70512-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     368-368: A → AESAMGGSVPSLRITSGGPQLSVPPLP

Show »
Length:491
Mass (Da):53,584
Checksum:iC0B87B4FB6F6C978
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei368 – 3681A → AESAMGGSVPSLRITSGGPQ LSVPPLP in isoform Beta. 1 PublicationVSP_002312

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96553 Genomic DNA. Translation: CAA65389.1.
Y14933 mRNA. Translation: CAA75150.1.
RefSeqiNP_073162.1. NM_022671.2. [P70512-1]
UniGeneiRn.48812.

Genome annotation databases

GeneIDi25231.
KEGGirno:25231.
UCSCiRGD:2811. rat. [P70512-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96553 Genomic DNA. Translation: CAA65389.1.
Y14933 mRNA. Translation: CAA75150.1.
RefSeqiNP_073162.1. NM_022671.2. [P70512-1]
UniGeneiRn.48812.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D5VX-ray2.00A/B289-444[»]
ProteinModelPortaliP70512.
SMRiP70512. Positions 291-437.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25231.
KEGGirno:25231.
UCSCiRGD:2811. rat. [P70512-1]

Organism-specific databases

CTDi3175.
RGDi2811. Onecut1.

Phylogenomic databases

HOGENOMiHOG000232114.
HOVERGENiHBG005981.
InParanoidiP70512.
KOiK08026.

Miscellaneous databases

EvolutionaryTraceiP70512.
PROiP70512.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR003350. CUT_dom.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF02376. CUT. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM01109. CUT. 1 hit.
SM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS51042. CUT. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Hepatocyte nuclear factor 6, a transcription factor that contains a novel type of homeodomain and a single cut domain."
    Lemaigre F.P., Durviaux S.M., Truong O., Lannoy V.J., Hsuan J.J., Rousseau G.G.
    Proc. Natl. Acad. Sci. U.S.A. 93:9460-9464(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM ALPHA).
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Isoforms of hepatocyte nuclear factor-6 differ in DNA-binding properties, contain a bifunctional homeodomain, and define the new ONECUT class of homeodomain proteins."
    Lannoy V.J., Buerglin T.R., Rousseau G.G., Lemaigre F.P.
    J. Biol. Chem. 273:13552-13562(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
    Strain: Wistar.
  3. "The transcriptional activator hepatocyte nuclear factor 6 regulates liver gene expression."
    Samadani U., Costa R.H.
    Mol. Cell. Biol. 16:6273-6284(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHNF6_RAT
AccessioniPrimary (citable) accession number: P70512
Secondary accession number(s): O88755
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: June 8, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.