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P70507

- GRK4_RAT

UniProt

P70507 - GRK4_RAT

Protein

G protein-coupled receptor kinase 4

Gene

Grk4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Specifically phosphorylates the activated forms of G protein-coupled receptors. Plays an important role in the regulation of renal sodium handling and blood pressure.1 Publication

    Catalytic activityi

    ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

    Enzyme regulationi

    Inhibited by heparin.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei215 – 2151ATPPROSITE-ProRule annotation
    Active sitei311 – 3111Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi192 – 2009ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. G-protein coupled receptor kinase activity Source: UniProtKB-EC
    3. rhodopsin kinase activity Source: RGD

    GO - Biological processi

    1. desensitization of G-protein coupled receptor protein signaling pathway Source: RGD
    2. G-protein coupled receptor internalization Source: RGD
    3. protein phosphorylation Source: RGD
    4. signal transduction Source: InterPro
    5. termination of G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G protein-coupled receptor kinase 4 (EC:2.7.11.16)
    Alternative name(s):
    G protein-coupled receptor kinase GRK4
    Gene namesi
    Name:Grk4
    Synonyms:Gprk2l
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 14

    Organism-specific databases

    RGDi61858. Grk4.

    Subcellular locationi

    Cytoplasm. Cytoplasmcell cortex By similarity

    GO - Cellular componenti

    1. cell cortex Source: UniProtKB-SubCell
    2. cytosol Source: RGD
    3. dendrite Source: RGD
    4. neuronal cell body Source: RGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 575575G protein-coupled receptor kinase 4PRO_0000085969Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Post-translational modificationi

    Palmitoylated.By similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP70507.
    PRIDEiP70507.

    PTM databases

    PhosphoSiteiP70507.

    Expressioni

    Tissue specificityi

    Isoform GRK4A is expressed in testis. Isoform GRK4B is heterogeneously distributed in the kidney, with 20-fold enrichment in the outer medulla. Has a widespread but low level of expression in tissues other than testis.1 Publication

    Gene expression databases

    GenevestigatoriP70507.

    Interactioni

    Subunit structurei

    Interacts with DRD3.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000015894.

    Structurei

    3D structure databases

    ProteinModelPortaliP70507.
    SMRiP70507. Positions 22-527.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini51 – 171121RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini186 – 448263Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini449 – 51466AGC-kinase C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 153153N-terminalAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00730000110512.
    HOGENOMiHOG000006742.
    HOVERGENiHBG004532.
    InParanoidiP70507.
    KOiK08291.
    OMAiSERLGCK.
    OrthoDBiEOG7V1FQK.
    PhylomeDBiP70507.
    TreeFamiTF313940.

    Family and domain databases

    InterProiIPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR00717. GPCRKINASE.
    SMARTiSM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform GRK4A (identifier: P70507-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MELENFMANT LLLKARQGFT KKTGRSKKWR ELLKLPPVSM CSDLRHSIEK    50
    DFSSLCDQQP IGRLLFRQFC NTKPDLKRCI EFLDAAAEYE VTIEEEQREF 100
    GLSIYSRFFK ENSEVSLPQI PPDLVKECKC NLKQSSPSQN VFQDCAGVIY 150
    KYLSEKPFEE YQESTYYNRF LQWKWLERRP VTKNTFRQYR VLGKGGFGEV 200
    CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKRILEK LHSRFVVSLA 250
    YTYETKDALC LVLTIMNGGD LKYHIYNLGN PGFEEQRAVF YAAELCCGLE 300
    DLQRERIVYR DLKPENILLD DHGHIRISDL GLALEIPEGE MVRGRVGTVG 350
    YMAPEIISHE KYTFSPDWWG LGCLIYEMIA GHSPFRKYKE KVNREEMERR 400
    VKTETEEYSE RFSENAKSIC SMLLTKDPSK RLGCQSDGAS AVKQHPIFKD 450
    INFSRLEANM LDPPFCPDPE AIYCKDILDI GQFSVVKGVN LDTNDEIFYT 500
    QFATGCVTIP WQNEMIESGC FKDLNEYEDK GLSPLEKHKI CSCILRPKRN 550
    FFHRLFRRAA CLNIAHSEER EPTEH 575
    Length:575
    Mass (Da):66,788
    Last modified:February 1, 1997 - v1
    Checksum:i4328ACD85D718560
    GO
    Isoform GRK4B (identifier: P70507-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         148-178: Missing.

    Show »
    Length:544
    Mass (Da):62,668
    Checksum:i7D57F96C831EE629
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei148 – 17831Missing in isoform GRK4B. 1 PublicationVSP_010352Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97568 mRNA. Translation: CAA66180.1.
    X97568 mRNA. Translation: CAA66181.1.
    RefSeqiNP_075217.1. NM_022928.1. [P70507-1]
    XP_006251421.1. XM_006251359.1. [P70507-2]
    UniGeneiRn.54517.

    Genome annotation databases

    EnsembliENSRNOT00000015894; ENSRNOP00000015894; ENSRNOG00000011847. [P70507-1]
    ENSRNOT00000016153; ENSRNOP00000016153; ENSRNOG00000011847. [P70507-2]
    GeneIDi59077.
    KEGGirno:59077.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97568 mRNA. Translation: CAA66180.1 .
    X97568 mRNA. Translation: CAA66181.1 .
    RefSeqi NP_075217.1. NM_022928.1. [P70507-1 ]
    XP_006251421.1. XM_006251359.1. [P70507-2 ]
    UniGenei Rn.54517.

    3D structure databases

    ProteinModelPortali P70507.
    SMRi P70507. Positions 22-527.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000015894.

    PTM databases

    PhosphoSitei P70507.

    Proteomic databases

    PaxDbi P70507.
    PRIDEi P70507.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000015894 ; ENSRNOP00000015894 ; ENSRNOG00000011847 . [P70507-1 ]
    ENSRNOT00000016153 ; ENSRNOP00000016153 ; ENSRNOG00000011847 . [P70507-2 ]
    GeneIDi 59077.
    KEGGi rno:59077.

    Organism-specific databases

    CTDi 2868.
    RGDi 61858. Grk4.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00730000110512.
    HOGENOMi HOG000006742.
    HOVERGENi HBG004532.
    InParanoidi P70507.
    KOi K08291.
    OMAi SERLGCK.
    OrthoDBi EOG7V1FQK.
    PhylomeDBi P70507.
    TreeFami TF313940.

    Miscellaneous databases

    NextBioi 611700.
    PROi P70507.

    Gene expression databases

    Genevestigatori P70507.

    Family and domain databases

    InterProi IPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00717. GPCRKINASE.
    SMARTi SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rat G protein-coupled receptor kinase GRK4: identification, functional expression, and differential tissue distribution of two splice variants."
      Virlon B., Firsov D., Cheval L., Reiter E., Troispoux C., Guillou F., Elalouf J.-M.
      Endocrinology 139:2784-2795(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS GRK4A AND GRK4B), CHARACTERIZATION, TISSUE SPECIFICITY.
    2. "Amelioration of genetic hypertension by suppression of renal G protein-coupled receptor kinase type 4 expression."
      Sanada H., Yatabe J., Midorikawa S., Katoh T., Hashimoto S., Watanabe T., Xu J., Luo Y., Wang X., Zeng C., Armando I., Felder R.A., Jose P.A.
      Hypertension 47:1131-1139(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiGRK4_RAT
    AccessioniPrimary (citable) accession number: P70507
    Secondary accession number(s): P70508
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3