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P70507

- GRK4_RAT

UniProt

P70507 - GRK4_RAT

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Protein

G protein-coupled receptor kinase 4

Gene

Grk4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Specifically phosphorylates the activated forms of G protein-coupled receptors. Plays an important role in the regulation of renal sodium handling and blood pressure.1 Publication

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Enzyme regulationi

Inhibited by heparin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei215 – 2151ATPPROSITE-ProRule annotation
Active sitei311 – 3111Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi192 – 2009ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. G-protein coupled receptor kinase activity Source: UniProtKB-EC
  3. rhodopsin kinase activity Source: RGD

GO - Biological processi

  1. desensitization of G-protein coupled receptor protein signaling pathway Source: RGD
  2. G-protein coupled receptor internalization Source: RGD
  3. protein phosphorylation Source: RGD
  4. signal transduction Source: InterPro
  5. termination of G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-coupled receptor kinase 4 (EC:2.7.11.16)
Alternative name(s):
G protein-coupled receptor kinase GRK4
Gene namesi
Name:Grk4
Synonyms:Gprk2l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 14

Organism-specific databases

RGDi61858. Grk4.

Subcellular locationi

Cytoplasm. Cytoplasmcell cortex By similarity

GO - Cellular componenti

  1. cytosol Source: RGD
  2. dendrite Source: RGD
  3. neuronal cell body Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 575575G protein-coupled receptor kinase 4PRO_0000085969Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Post-translational modificationi

Palmitoylated.By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP70507.
PRIDEiP70507.

PTM databases

PhosphoSiteiP70507.

Expressioni

Tissue specificityi

Isoform GRK4A is expressed in testis. Isoform GRK4B is heterogeneously distributed in the kidney, with 20-fold enrichment in the outer medulla. Has a widespread but low level of expression in tissues other than testis.1 Publication

Gene expression databases

GenevestigatoriP70507.

Interactioni

Subunit structurei

Interacts with DRD3.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015894.

Structurei

3D structure databases

ProteinModelPortaliP70507.
SMRiP70507. Positions 22-527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 171121RGSPROSITE-ProRule annotationAdd
BLAST
Domaini186 – 448263Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini449 – 51466AGC-kinase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 153153N-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120493.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiP70507.
KOiK08291.
OMAiSERLGCK.
OrthoDBiEOG7V1FQK.
PhylomeDBiP70507.
TreeFamiTF313940.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform GRK4A (identifier: P70507-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELENFMANT LLLKARQGFT KKTGRSKKWR ELLKLPPVSM CSDLRHSIEK
60 70 80 90 100
DFSSLCDQQP IGRLLFRQFC NTKPDLKRCI EFLDAAAEYE VTIEEEQREF
110 120 130 140 150
GLSIYSRFFK ENSEVSLPQI PPDLVKECKC NLKQSSPSQN VFQDCAGVIY
160 170 180 190 200
KYLSEKPFEE YQESTYYNRF LQWKWLERRP VTKNTFRQYR VLGKGGFGEV
210 220 230 240 250
CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKRILEK LHSRFVVSLA
260 270 280 290 300
YTYETKDALC LVLTIMNGGD LKYHIYNLGN PGFEEQRAVF YAAELCCGLE
310 320 330 340 350
DLQRERIVYR DLKPENILLD DHGHIRISDL GLALEIPEGE MVRGRVGTVG
360 370 380 390 400
YMAPEIISHE KYTFSPDWWG LGCLIYEMIA GHSPFRKYKE KVNREEMERR
410 420 430 440 450
VKTETEEYSE RFSENAKSIC SMLLTKDPSK RLGCQSDGAS AVKQHPIFKD
460 470 480 490 500
INFSRLEANM LDPPFCPDPE AIYCKDILDI GQFSVVKGVN LDTNDEIFYT
510 520 530 540 550
QFATGCVTIP WQNEMIESGC FKDLNEYEDK GLSPLEKHKI CSCILRPKRN
560 570
FFHRLFRRAA CLNIAHSEER EPTEH
Length:575
Mass (Da):66,788
Last modified:February 1, 1997 - v1
Checksum:i4328ACD85D718560
GO
Isoform GRK4B (identifier: P70507-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     148-178: Missing.

Show »
Length:544
Mass (Da):62,668
Checksum:i7D57F96C831EE629
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei148 – 17831Missing in isoform GRK4B. 1 PublicationVSP_010352Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97568 mRNA. Translation: CAA66180.1.
X97568 mRNA. Translation: CAA66181.1.
RefSeqiNP_075217.1. NM_022928.1. [P70507-1]
XP_006251421.1. XM_006251359.1. [P70507-2]
UniGeneiRn.54517.

Genome annotation databases

EnsembliENSRNOT00000015894; ENSRNOP00000015894; ENSRNOG00000011847. [P70507-1]
ENSRNOT00000016153; ENSRNOP00000016153; ENSRNOG00000011847. [P70507-2]
GeneIDi59077.
KEGGirno:59077.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97568 mRNA. Translation: CAA66180.1 .
X97568 mRNA. Translation: CAA66181.1 .
RefSeqi NP_075217.1. NM_022928.1. [P70507-1 ]
XP_006251421.1. XM_006251359.1. [P70507-2 ]
UniGenei Rn.54517.

3D structure databases

ProteinModelPortali P70507.
SMRi P70507. Positions 22-527.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000015894.

PTM databases

PhosphoSitei P70507.

Proteomic databases

PaxDbi P70507.
PRIDEi P70507.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000015894 ; ENSRNOP00000015894 ; ENSRNOG00000011847 . [P70507-1 ]
ENSRNOT00000016153 ; ENSRNOP00000016153 ; ENSRNOG00000011847 . [P70507-2 ]
GeneIDi 59077.
KEGGi rno:59077.

Organism-specific databases

CTDi 2868.
RGDi 61858. Grk4.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00770000120493.
HOGENOMi HOG000006742.
HOVERGENi HBG004532.
InParanoidi P70507.
KOi K08291.
OMAi SERLGCK.
OrthoDBi EOG7V1FQK.
PhylomeDBi P70507.
TreeFami TF313940.

Miscellaneous databases

NextBioi 611700.
PROi P70507.

Gene expression databases

Genevestigatori P70507.

Family and domain databases

InterProi IPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view ]
PRINTSi PR00717. GPCRKINASE.
SMARTi SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Rat G protein-coupled receptor kinase GRK4: identification, functional expression, and differential tissue distribution of two splice variants."
    Virlon B., Firsov D., Cheval L., Reiter E., Troispoux C., Guillou F., Elalouf J.-M.
    Endocrinology 139:2784-2795(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS GRK4A AND GRK4B), CHARACTERIZATION, TISSUE SPECIFICITY.
  2. "Amelioration of genetic hypertension by suppression of renal G protein-coupled receptor kinase type 4 expression."
    Sanada H., Yatabe J., Midorikawa S., Katoh T., Hashimoto S., Watanabe T., Xu J., Luo Y., Wang X., Zeng C., Armando I., Felder R.A., Jose P.A.
    Hypertension 47:1131-1139(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGRK4_RAT
AccessioniPrimary (citable) accession number: P70507
Secondary accession number(s): P70508
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: February 1, 1997
Last modified: November 26, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3