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P70507 (GRK4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
G protein-coupled receptor kinase 4
EC=2.7.11.16
Alternative name(s):
G protein-coupled receptor kinase GRK4
Gene names
Name:Grk4
Synonyms:Gprk2l
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length575 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically phosphorylates the activated forms of G protein-coupled receptors. Plays an important role in the regulation of renal sodium handling and blood pressure. Ref.2

Catalytic activity

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Enzyme regulation

Inhibited by heparin By similarity.

Subunit structure

Interacts with DRD3 By similarity.

Subcellular location

Cytoplasm. Cytoplasm › cell cortex By similarity.

Tissue specificity

Isoform GRK4A is expressed in testis. Isoform GRK4B is heterogeneously distributed in the kidney, with 20-fold enrichment in the outer medulla. Has a widespread but low level of expression in tissues other than testis. Ref.1

Post-translational modification

Palmitoylated By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Contains 1 RGS domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform GRK4A (identifier: P70507-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform GRK4B (identifier: P70507-2)

The sequence of this isoform differs from the canonical sequence as follows:
     148-178: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 575575G protein-coupled receptor kinase 4
PRO_0000085969

Regions

Domain51 – 171121RGS
Domain186 – 448263Protein kinase
Domain449 – 51466AGC-kinase C-terminal
Nucleotide binding192 – 2009ATP By similarity
Region1 – 153153N-terminal

Sites

Active site3111Proton acceptor By similarity
Binding site2151ATP By similarity

Natural variations

Alternative sequence148 – 17831Missing in isoform GRK4B.
VSP_010352

Sequences

Sequence LengthMass (Da)Tools
Isoform GRK4A [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 4328ACD85D718560

FASTA57566,788
        10         20         30         40         50         60 
MELENFMANT LLLKARQGFT KKTGRSKKWR ELLKLPPVSM CSDLRHSIEK DFSSLCDQQP 

        70         80         90        100        110        120 
IGRLLFRQFC NTKPDLKRCI EFLDAAAEYE VTIEEEQREF GLSIYSRFFK ENSEVSLPQI 

       130        140        150        160        170        180 
PPDLVKECKC NLKQSSPSQN VFQDCAGVIY KYLSEKPFEE YQESTYYNRF LQWKWLERRP 

       190        200        210        220        230        240 
VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKRILEK 

       250        260        270        280        290        300 
LHSRFVVSLA YTYETKDALC LVLTIMNGGD LKYHIYNLGN PGFEEQRAVF YAAELCCGLE 

       310        320        330        340        350        360 
DLQRERIVYR DLKPENILLD DHGHIRISDL GLALEIPEGE MVRGRVGTVG YMAPEIISHE 

       370        380        390        400        410        420 
KYTFSPDWWG LGCLIYEMIA GHSPFRKYKE KVNREEMERR VKTETEEYSE RFSENAKSIC 

       430        440        450        460        470        480 
SMLLTKDPSK RLGCQSDGAS AVKQHPIFKD INFSRLEANM LDPPFCPDPE AIYCKDILDI 

       490        500        510        520        530        540 
GQFSVVKGVN LDTNDEIFYT QFATGCVTIP WQNEMIESGC FKDLNEYEDK GLSPLEKHKI 

       550        560        570 
CSCILRPKRN FFHRLFRRAA CLNIAHSEER EPTEH

« Hide

Isoform GRK4B [UniParc].

Checksum: 7D57F96C831EE629
Show »

FASTA54462,668

References

[1]"Rat G protein-coupled receptor kinase GRK4: identification, functional expression, and differential tissue distribution of two splice variants."
Virlon B., Firsov D., Cheval L., Reiter E., Troispoux C., Guillou F., Elalouf J.-M.
Endocrinology 139:2784-2795(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS GRK4A AND GRK4B), CHARACTERIZATION, TISSUE SPECIFICITY.
[2]"Amelioration of genetic hypertension by suppression of renal G protein-coupled receptor kinase type 4 expression."
Sanada H., Yatabe J., Midorikawa S., Katoh T., Hashimoto S., Watanabe T., Xu J., Luo Y., Wang X., Zeng C., Armando I., Felder R.A., Jose P.A.
Hypertension 47:1131-1139(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X97568 mRNA. Translation: CAA66180.1.
X97568 mRNA. Translation: CAA66181.1.
IPIIPI00189571.
IPI00332016.
RefSeqNP_075217.1. NM_022928.1.
UniGeneRn.54517.

3D structure databases

ProteinModelPortalP70507.
SMRP70507. Positions 22-527.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000015894.

PTM databases

PhosphoSiteP70507.

Proteomic databases

PaxDbP70507.
PRIDEP70507.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000015894; ENSRNOP00000015894; ENSRNOG00000011847.
ENSRNOT00000016153; ENSRNOP00000016153; ENSRNOG00000011847.
GeneID59077.
KEGGrno:59077.

Organism-specific databases

CTD2868.
RGD61858. Grk4.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000074318.
HOGENOMHOG000006742.
HOVERGENHBG004532.
InParanoidP70507.
KOK08291.
OMAHSIEKDY.
OrthoDBEOG4HHP24.

Gene expression databases

GenevestigatorP70507.
GermOnlineENSRNOG00000011847. Rattus norvegicus.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR000342. Regulat_G_prot_signal.
IPR016137. Regulat_G_prot_signal_superfam.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR00717. GPCRKINASE.
SMARTSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio611700.

Entry information

Entry nameGRK4_RAT
AccessionPrimary (citable) accession number: P70507
Secondary accession number(s): P70508
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: February 1, 1997
Last modified: April 3, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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