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Protein

G protein-coupled receptor kinase 4

Gene

Grk4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically phosphorylates the activated forms of G protein-coupled receptors. Plays an important role in the regulation of renal sodium handling and blood pressure.1 Publication

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Enzyme regulationi

Inhibited by heparin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215ATPPROSITE-ProRule annotation1
Active sitei311Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi192 – 200ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • G-protein coupled receptor kinase activity Source: UniProtKB-EC
  • rhodopsin kinase activity Source: RGD

GO - Biological processi

  • desensitization of G-protein coupled receptor protein signaling pathway Source: RGD
  • G-protein coupled receptor internalization Source: RGD
  • protein phosphorylation Source: RGD
  • signal transduction Source: InterPro

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-2514859 Inactivation, recovery and regulation of the phototransduction cascade

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-coupled receptor kinase 4 (EC:2.7.11.16)
Alternative name(s):
G protein-coupled receptor kinase GRK4
Gene namesi
Name:Grk4
Synonyms:Gprk2l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi61858 Grk4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859691 – 575G protein-coupled receptor kinase 4Add BLAST575

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei484PhosphoserineCombined sources1

Post-translational modificationi

Palmitoylated.By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP70507
PRIDEiP70507

PTM databases

iPTMnetiP70507
PhosphoSitePlusiP70507

Expressioni

Tissue specificityi

Isoform GRK4A is expressed in testis. Isoform GRK4B is heterogeneously distributed in the kidney, with 20-fold enrichment in the outer medulla. Has a widespread but low level of expression in tissues other than testis.1 Publication

Gene expression databases

BgeeiENSRNOG00000011847
GenevisibleiP70507 RN

Interactioni

Subunit structurei

Interacts with DRD3.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015894

Structurei

3D structure databases

ProteinModelPortaliP70507
SMRiP70507
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 171RGSPROSITE-ProRule annotationAdd BLAST121
Domaini186 – 448Protein kinasePROSITE-ProRule annotationAdd BLAST263
Domaini449 – 514AGC-kinase C-terminalAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 153N-terminalAdd BLAST153

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0986 Eukaryota
ENOG410YRQZ LUCA
GeneTreeiENSGT00860000133699
HOGENOMiHOG000006742
HOVERGENiHBG004532
InParanoidiP70507
KOiK08291
OMAiHSIEKDY
OrthoDBiEOG091G062G
PhylomeDBiP70507
TreeFamiTF313940

Family and domain databases

InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR000239 GPCR_kinase
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR016137 RGS
IPR036305 RGS_sf
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00615 RGS, 1 hit
PRINTSiPR00717 GPCRKINASE
SMARTiView protein in SMART
SM00315 RGS, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF48097 SSF48097, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50132 RGS, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform GRK4A (identifier: P70507-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELENFMANT LLLKARQGFT KKTGRSKKWR ELLKLPPVSM CSDLRHSIEK
60 70 80 90 100
DFSSLCDQQP IGRLLFRQFC NTKPDLKRCI EFLDAAAEYE VTIEEEQREF
110 120 130 140 150
GLSIYSRFFK ENSEVSLPQI PPDLVKECKC NLKQSSPSQN VFQDCAGVIY
160 170 180 190 200
KYLSEKPFEE YQESTYYNRF LQWKWLERRP VTKNTFRQYR VLGKGGFGEV
210 220 230 240 250
CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKRILEK LHSRFVVSLA
260 270 280 290 300
YTYETKDALC LVLTIMNGGD LKYHIYNLGN PGFEEQRAVF YAAELCCGLE
310 320 330 340 350
DLQRERIVYR DLKPENILLD DHGHIRISDL GLALEIPEGE MVRGRVGTVG
360 370 380 390 400
YMAPEIISHE KYTFSPDWWG LGCLIYEMIA GHSPFRKYKE KVNREEMERR
410 420 430 440 450
VKTETEEYSE RFSENAKSIC SMLLTKDPSK RLGCQSDGAS AVKQHPIFKD
460 470 480 490 500
INFSRLEANM LDPPFCPDPE AIYCKDILDI GQFSVVKGVN LDTNDEIFYT
510 520 530 540 550
QFATGCVTIP WQNEMIESGC FKDLNEYEDK GLSPLEKHKI CSCILRPKRN
560 570
FFHRLFRRAA CLNIAHSEER EPTEH
Length:575
Mass (Da):66,788
Last modified:February 1, 1997 - v1
Checksum:i4328ACD85D718560
GO
Isoform GRK4B (identifier: P70507-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     148-178: Missing.

Show »
Length:544
Mass (Da):62,668
Checksum:i7D57F96C831EE629
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010352148 – 178Missing in isoform GRK4B. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97568 mRNA Translation: CAA66180.1
X97568 mRNA Translation: CAA66181.1
RefSeqiNP_075217.1, NM_022928.1 [P70507-1]
XP_006251421.1, XM_006251359.2 [P70507-2]
UniGeneiRn.54517

Genome annotation databases

EnsembliENSRNOT00000015894; ENSRNOP00000015894; ENSRNOG00000011847 [P70507-1]
GeneIDi59077
KEGGirno:59077

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGRK4_RAT
AccessioniPrimary (citable) accession number: P70507
Secondary accession number(s): P70508
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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