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Protein

CDP-diacylglycerol--inositol 3-phosphatidyltransferase

Gene

Cdipt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme.2 Publications

Catalytic activityi

CDP-diacylglycerol + myo-inositol = CMP + phosphatidyl-1D-myo-inositol.2 Publications

Cofactori

Mn2+2 Publications, Mg2+2 PublicationsNote: Divalent metal cations; Mn2+ or Mg2+ (PubMed:3032971). According to PubMed:7998949 the cofactor is Mn2+, while Mg2+ is much less effective.2 Publications

GO - Molecular functioni

  • alcohol binding Source: RGD
  • carbohydrate binding Source: RGD
  • CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity Source: RGD
  • diacylglycerol binding Source: RGD
  • manganese ion binding Source: RGD

GO - Biological processi

  • CDP-diacylglycerol metabolic process Source: RGD
  • phosphatidylinositol biosynthetic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

Magnesium, Manganese

Enzyme and pathway databases

ReactomeiR-RNO-1483226. Synthesis of PI.

Names & Taxonomyi

Protein namesi
Recommended name:
CDP-diacylglycerol--inositol 3-phosphatidyltransferase (EC:2.7.8.11)
Alternative name(s):
Phosphatidylinositol synthase
Short name:
PI synthase
Short name:
PtdIns synthase
Gene namesi
Name:Cdipt
Synonyms:Pis1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620576. Cdipt.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalSequence analysisAdd
BLAST
Transmembranei29 – 4921HelicalSequence analysisAdd
BLAST
Transmembranei74 – 9421HelicalSequence analysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence analysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213CDP-diacylglycerol--inositol 3-phosphatidyltransferasePRO_0000056804Add
BLAST

Proteomic databases

PaxDbiP70500.
PRIDEiP70500.

PTM databases

iPTMnetiP70500.
PhosphoSiteiP70500.

Expressioni

Tissue specificityi

Detected in liver (at protein level). Widely expressed. Highly expressed in the brain and kidney; lower levels in heart, spleen, lung, liver, skeletal muscle and testis.1 Publication

Inductioni

Inhibited by PtdIns (product inhibition).

Gene expression databases

BgeeiENSRNOG00000024144.
GenevisibleiP70500. RN.

Interactioni

Protein-protein interaction databases

IntActiP70500. 1 interaction.
MINTiMINT-4568435.
STRINGi10116.ENSRNOP00000013691.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3240. Eukaryota.
COG0558. LUCA.
GeneTreeiENSGT00390000017279.
HOGENOMiHOG000204051.
HOVERGENiHBG050862.
InParanoidiP70500.
KOiK00999.
OMAiTDRCGTM.
PhylomeDBiP70500.
TreeFamiTF314603.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014387. CDP_diag_ino_3_P_euk.
[Graphical view]
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000848. CDP_diag_ino_3_P. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70500-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEENIFLFV PNLIGYARIV FAIISFYFMP CCPFTASSFY LLSGLLDAFD
60 70 80 90 100
GHAARALNQG TRFGAMLDML TDRCATMCLL VNLALLYPRA TLLFQLSMSL
110 120 130 140 150
DVASHWLHLH SSVVRGSESH KMIDLSGNPV LRIYYTSRPA LFTLCAGNEL
160 170 180 190 200
FYCLLYLFNF SEGPLVGSVG LFRMGLWITA PIALLKSIIS VIHLVTAARN
210
MAALDAADRA KKK
Length:213
Mass (Da):23,613
Last modified:February 1, 1997 - v1
Checksum:i93C7137664EAABB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82928 mRNA. Translation: BAA11634.1.
AB022890 Genomic DNA. Translation: BAA82112.1.
BC070876 mRNA. Translation: AAH70876.1.
PIRiS74247.
RefSeqiNP_620254.1. NM_138899.2.
XP_006230270.1. XM_006230208.1.
UniGeneiRn.10598.

Genome annotation databases

EnsembliENSRNOT00000013691; ENSRNOP00000013691; ENSRNOG00000024144.
ENSRNOT00000091295; ENSRNOP00000070871; ENSRNOG00000024144.
GeneIDi192260.
KEGGirno:192260.
UCSCiRGD:620576. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82928 mRNA. Translation: BAA11634.1.
AB022890 Genomic DNA. Translation: BAA82112.1.
BC070876 mRNA. Translation: AAH70876.1.
PIRiS74247.
RefSeqiNP_620254.1. NM_138899.2.
XP_006230270.1. XM_006230208.1.
UniGeneiRn.10598.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP70500. 1 interaction.
MINTiMINT-4568435.
STRINGi10116.ENSRNOP00000013691.

PTM databases

iPTMnetiP70500.
PhosphoSiteiP70500.

Proteomic databases

PaxDbiP70500.
PRIDEiP70500.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013691; ENSRNOP00000013691; ENSRNOG00000024144.
ENSRNOT00000091295; ENSRNOP00000070871; ENSRNOG00000024144.
GeneIDi192260.
KEGGirno:192260.
UCSCiRGD:620576. rat.

Organism-specific databases

CTDi10423.
RGDi620576. Cdipt.

Phylogenomic databases

eggNOGiKOG3240. Eukaryota.
COG0558. LUCA.
GeneTreeiENSGT00390000017279.
HOGENOMiHOG000204051.
HOVERGENiHBG050862.
InParanoidiP70500.
KOiK00999.
OMAiTDRCGTM.
PhylomeDBiP70500.
TreeFamiTF314603.

Enzyme and pathway databases

ReactomeiR-RNO-1483226. Synthesis of PI.

Miscellaneous databases

PROiP70500.

Gene expression databases

BgeeiENSRNOG00000024144.
GenevisibleiP70500. RN.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014387. CDP_diag_ino_3_P_euk.
[Graphical view]
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000848. CDP_diag_ino_3_P. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDIPT_RAT
AccessioniPrimary (citable) accession number: P70500
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.