Phospholipase D1 - Rattus norvegicus (Rat)

Names and origin

Protein names

Recommended name:
    Phospholipase D1
      Short name=PLD 1
      Short name=rPLD1
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 1
    Phosphatidylcholine-hydrolyzing phospholipase D1

Gene names

Name:

Pld1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	1074 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic By similarity.
Catalytic activity	A phosphatidylcholine + H₂O = choline + a phosphatidate.
Enzyme regulation	Stimulated by phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate, activated by the phosphokinase C-alpha, by the ADP-ribosylation factor-1 (ARF-1), and to a lesser extent by GTP-binding proteins: RHO A, RAC-1 and CDC42 By similarity.
Subunit structure	Interacts with PIP5K1A By similarity.
Subcellular location	Cytoplasm › perinuclear region By similarity. Endoplasmic reticulum membrane; Lipid-anchor By similarity. Golgi apparatus membrane; Lipid-anchor By similarity. Late endosome membrane; Lipid-anchor By similarity. Note: Membrane-associated.
Post-translational modification	Phosphorylated on serine and threonine residues. Ref.7 It is uncertain whether palmitoylation is on Cys-240 and/or Cys-241. Palmitoylation is required prior to phosphorylation.
Sequence similarities	Belongs to the phospholipase D family. Contains 1 PH domain. Contains 2 PLD phosphodiesterase domains. Contains 1 PX (phox homology) domain.

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Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cytoplasm Endoplasmic reticulum Endosome Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Molecular function	Hydrolase
PTM	Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	cell communication Inferred from electronic annotation. Source: InterPro phosphatidic acid biosynthetic process Inferred from mutant phenotype. Source: MGI phospholipid catabolic process Inferred from mutant phenotype. Source: RGD positive regulation of cell migration Inferred from mutant phenotype. Source: RGD response to peptide hormone stimulus Inferred from mutant phenotype. Source: RGD
Cellular component	Golgi cisterna Inferred from direct assay. Source: RGD anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW lamellipodium Inferred from direct assay. Source: RGD microsome Inferred from direct assay. Source: RGD perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell vesicle Inferred from direct assay. Source: RGD
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phosphoinositide binding Inferred from electronic annotation. Source: InterPro phospholipase D activity Inferred from direct assay. Source: RGD protein binding Inferred from direct assay. Source: RGD
Complete GO annotation...

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform PLD1A (identifier: P70496-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform PLD1B (identifier: P70496-2) The sequence of this isoform differs from the canonical sequence as follows: 585-623: SYFNHYRSHQNLIHGIKPHLKLFRPSSESEQGLTRHSAD → N

Isoform PLD1C (identifier: P70496-3) The sequence of this isoform differs from the canonical sequence as follows: 586-590: YFNHY → ESRLR 591-1074: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1074

1074

Phospholipase D1

PRO_0000218804

Regions

Domain

81 – 212

132

PX

Domain

219 – 328

110

PH

Domain

459 – 486

28

PLD phosphodiesterase 1

Domain

891 – 918

28

PLD phosphodiesterase 2

Region

463 – 928

466

Catalytic

Amino acid modifications

Modified residue

629

1

Phosphoserine By similarity

Lipidation

240

1

S-palmitoyl cysteine Probable

Lipidation

241

1

S-palmitoyl cysteine Probable

Natural variations

Alternative sequence

585 – 623

39

SYFNH…RHSAD → N in isoform PLD1B.

VSP_005024

Alternative sequence

586 – 590

5

YFNHY → ESRLR in isoform PLD1C.

VSP_005025

Alternative sequence

591 – 1074

484

Missing in isoform PLD1C.

VSP_005026

Experimental info

Mutagenesis

240

1

C → A: Abolishes palmitoylation and weakens membrane association; when associated with A-241. Ref.7

Mutagenesis

241

1

C → A: Abolishes palmitoylation and weakens membrane association; when associated with A-240. Ref.7

Sequence conflict

4

1

R → K in BAA24576. Ref.4

Sequence conflict

22

1

S → R Ref.3

Sequence conflict

22

1

S → R Ref.6

Sequence conflict

283

1

R → K in BAA24076. Ref.1

Sequence conflict

283

1

R → K in BAA24077. Ref.1

Sequence conflict

542

1

N → D Ref.1

Sequence conflict

542

1

N → D Ref.2

Sequence conflict

542

1

N → D Ref.4

Sequence conflict

600

1

I → L in BAA24576. Ref.4

Sequence conflict

709

1

P → A in BAA24076. Ref.1

Sequence conflict

709

1

P → A in BAA24077. Ref.1

Sequence conflict

735 – 736

2

PG → EV in BAA24076. Ref.1

Sequence conflict

735 – 736

2

PG → EV in BAA24077. Ref.1

Sequence conflict

739 – 740

2

HA → QP in BAA24076. Ref.1

Sequence conflict

739 – 740

2

HA → QP in BAA24077. Ref.1

Sequence conflict

773

1

S → T Ref.1

Sequence conflict

775

1

H → R Ref.1

Sequence conflict

786

1

S → T in BAA24076. Ref.1

Sequence conflict

786

1

S → T in BAA24077. Ref.1

Sequence conflict

800

1

A → C in BAA24076. Ref.1

Sequence conflict

800

1

A → C in BAA24077. Ref.1

Sequence conflict

809

1

H → T in BAA24076. Ref.1

Sequence conflict

809

1

H → T in BAA24077. Ref.1

Sequence conflict

830

1

D → N in BAA24076. Ref.1

Sequence conflict

830

1

D → N in BAA24077. Ref.1

Sequence conflict

934 – 935

2

TE → RQ in AAB86910. Ref.3

Sequence conflict

951 – 954

4

FAQG → SLSTV Ref.1

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Sequences

Sequence		Length	Mass (Da)
Isoform PLD1A [UniParc]. Last modified October 18, 2001. Version 3. Checksum: 286943A447A881DB Show »	FASTA	1,074	123,814
10 20 30 40 50 60 MSLRSEARVN TSTLQKIAAD MSNLIENLDT RELHFEGEEV EYDASPGDPT AQEACIPFSS 70 80 90 100 110 120 IYNTQGFKEP NIQIYLSGCP VKAQVLEVER FTSTSRMPSV NLYTIELTHG EFTWQVKRKF 130 140 150 160 170 180 KHFQEFHREL LKYKAFIRIP IPTKRHTFRR QNVKEEPREM PSLPRSSENA IQEEQFFGRR 190 200 210 220 230 240 KQLEDYLTKI LKMPMYRNYH ATTEFLDVSQ LSFIHDLGPK GLEGMIMKRS GGHRIPGVNC 250 260 270 280 290 300 CGHGRACYRW SKRWLIVKDS FLLYMKPDSG AIAFVLLVDK EFRIKVGKKE TETKYGLRID 310 320 330 340 350 360 NLSRTLILKC NSYRHARWWG GAIEEFIQKH GTDFLKDHRF GSYAAVHENI LAKWYVNAKG 370 380 390 400 410 420 YFEDIANAME GATEEIFITD WWLSPEIFLK RPVVEGNRWR LDCILKRKAQ QGVRIFIMLY 430 440 450 460 470 480 KEVELALGIN SEYTKRTLMR LHPNIKVMRH PDHVSSSVYL WAHHEKLVII DQSVAFVGGI 490 500 510 520 530 540 DLAYGRWDDN EHRLTDVGSV KRVTSGQSLG SLTAASVESM ESLSLKDKHQ SHKNEPVLKS 550 560 570 580 590 600 VNDTDMKLKG IGKSRKFSKF SLYRQLHRRN LHNSDSISSV DSASSYFNHY RSHQNLIHGI 610 620 630 640 650 660 KPHLKLFRPS SESEQGLTRH SADTGSIRSV QTGVGELHGE TRFWHGKDYC NFVFKDWVQL 670 680 690 700 710 720 DKPFADFIDR YSTPRMPWHD IGSVVHGKAA RDVARHFIQR WNFTKIMKPK YRSLSYPFLL 730 740 750 760 770 780 PKSQATAHEL RYQVPGAVHA KAQLLRSAAD WSAGIKHHEE SIHAAYTHVI ENSKHYIYIE 790 800 810 820 830 840 NQFFISCADD KVVFNKVGNA IAQRILKAHR EGQRYRVYIV IPLLPGFEGD ISTGGGNALQ 850 860 870 880 890 900 AIMHFNYRTM CRGESSILEQ LKPELGNKWI NYISFCGLRT HAELEGNLVT ELIYVHSKLL 910 920 930 940 950 960 IADDNTVIIG SANINDRSML GKRDSEMAVI VQDTETVPSV MDGKEYQAGR FAQGLRLECF 970 980 990 1000 1010 1020 RLVLGYLSDP SEDIQDPVSD KFFKEIWVST AARNATIYDK VFRCLPNDEV HNLIQLRDFI 1030 1040 1050 1060 1070 NKPILAKEDR LRAEEELRKI RGFLVQFPFY FLSEENLLPS VGTKEAIVPM EVWT « Hide
Isoform PLD1B. Checksum: 350956C43A38E506 Show »	FASTA	1,036	119,357
10 20 30 40 50 60 MSLRSEARVN TSTLQKIAAD MSNLIENLDT RELHFEGEEV EYDASPGDPT AQEACIPFSS 70 80 90 100 110 120 IYNTQGFKEP NIQIYLSGCP VKAQVLEVER FTSTSRMPSV NLYTIELTHG EFTWQVKRKF 130 140 150 160 170 180 KHFQEFHREL LKYKAFIRIP IPTKRHTFRR QNVKEEPREM PSLPRSSENA IQEEQFFGRR 190 200 210 220 230 240 KQLEDYLTKI LKMPMYRNYH ATTEFLDVSQ LSFIHDLGPK GLEGMIMKRS GGHRIPGVNC 250 260 270 280 290 300 CGHGRACYRW SKRWLIVKDS FLLYMKPDSG AIAFVLLVDK EFRIKVGKKE TETKYGLRID 310 320 330 340 350 360 NLSRTLILKC NSYRHARWWG GAIEEFIQKH GTDFLKDHRF GSYAAVHENI LAKWYVNAKG 370 380 390 400 410 420 YFEDIANAME GATEEIFITD WWLSPEIFLK RPVVEGNRWR LDCILKRKAQ QGVRIFIMLY 430 440 450 460 470 480 KEVELALGIN SEYTKRTLMR LHPNIKVMRH PDHVSSSVYL WAHHEKLVII DQSVAFVGGI 490 500 510 520 530 540 DLAYGRWDDN EHRLTDVGSV KRVTSGQSLG SLTAASVESM ESLSLKDKHQ SHKNEPVLKS 550 560 570 580 590 600 VNDTDMKLKG IGKSRKFSKF SLYRQLHRRN LHNSDSISSV DSASNTGSIR SVQTGVGELH 610 620 630 640 650 660 GETRFWHGKD YCNFVFKDWV QLDKPFADFI DRYSTPRMPW HDIGSVVHGK AARDVARHFI 670 680 690 700 710 720 QRWNFTKIMK PKYRSLSYPF LLPKSQATAH ELRYQVPGAV HAKAQLLRSA ADWSAGIKHH 730 740 750 760 770 780 EESIHAAYTH VIENSKHYIY IENQFFISCA DDKVVFNKVG NAIAQRILKA HREGQRYRVY 790 800 810 820 830 840 IVIPLLPGFE GDISTGGGNA LQAIMHFNYR TMCRGESSIL EQLKPELGNK WINYISFCGL 850 860 870 880 890 900 RTHAELEGNL VTELIYVHSK LLIADDNTVI IGSANINDRS MLGKRDSEMA VIVQDTETVP 910 920 930 940 950 960 SVMDGKEYQA GRFAQGLRLE CFRLVLGYLS DPSEDIQDPV SDKFFKEIWV STAARNATIY 970 980 990 1000 1010 1020 DKVFRCLPND EVHNLIQLRD FINKPILAKE DRLRAEEELR KIRGFLVQFP FYFLSEENLL 1030 PSVGTKEAIV PMEVWT « Hide
Isoform PLD1C. Checksum: FBD1FD4D7FAF9D18 Show »	FASTA	590	68,318
10 20 30 40 50 60 MSLRSEARVN TSTLQKIAAD MSNLIENLDT RELHFEGEEV EYDASPGDPT AQEACIPFSS 70 80 90 100 110 120 IYNTQGFKEP NIQIYLSGCP VKAQVLEVER FTSTSRMPSV NLYTIELTHG EFTWQVKRKF 130 140 150 160 170 180 KHFQEFHREL LKYKAFIRIP IPTKRHTFRR QNVKEEPREM PSLPRSSENA IQEEQFFGRR 190 200 210 220 230 240 KQLEDYLTKI LKMPMYRNYH ATTEFLDVSQ LSFIHDLGPK GLEGMIMKRS GGHRIPGVNC 250 260 270 280 290 300 CGHGRACYRW SKRWLIVKDS FLLYMKPDSG AIAFVLLVDK EFRIKVGKKE TETKYGLRID 310 320 330 340 350 360 NLSRTLILKC NSYRHARWWG GAIEEFIQKH GTDFLKDHRF GSYAAVHENI LAKWYVNAKG 370 380 390 400 410 420 YFEDIANAME GATEEIFITD WWLSPEIFLK RPVVEGNRWR LDCILKRKAQ QGVRIFIMLY 430 440 450 460 470 480 KEVELALGIN SEYTKRTLMR LHPNIKVMRH PDHVSSSVYL WAHHEKLVII DQSVAFVGGI 490 500 510 520 530 540 DLAYGRWDDN EHRLTDVGSV KRVTSGQSLG SLTAASVESM ESLSLKDKHQ SHKNEPVLKS 550 560 570 580 590 VNDTDMKLKG IGKSRKFSKF SLYRQLHRRN LHNSDSISSV DSASSESRLR « Hide

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References

[1]	"Molecular cloning and chromosome mapping of rat phospholipase D genes, Pld1a, Pld1b and Pld2." Nakashima S., Matsuda Y., Akao Y., Yoshimura S., Sakai H., Hayakawa K., Andoh M., Nozawa Y. Cytogenet. Cell Genet. 79:109-113(1997) [PubMed: 9533024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS PLD1A AND PLD1B).
[2]	"Differential mRNA expression of phospholipase D (PLD) isozymes during cAMP-induced differentiation in C6 glioma cells." Yoshimura S., Nakashima S., Ohguchi K., Sakai H., Shinoda J., Sakai N., Nozawa Y. Biochem. Biophys. Res. Commun. 225:494-499(1996) [PubMed: 8753790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 467-673 (ISOFORMS PLD1A AND PLD1B). Tissue: Glial cell.
[3]	"Cloning and characterization of phospholipase D from rat brain." Park S.K., Provost J.J., Bae C.D., Ho W.T., Exton J.H. J. Biol. Chem. 272:29263-29271(1997) [PubMed: 9361006] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PLD1B).
[4]	"Cloning, differential regulation and tissue distribution of alternatively spliced isoforms of ADP-ribosylation-factor-dependent phospholipase D from rat liver." Katayama K., Kodaki T., Nagamachi Y., Yamashita S. Biochem. J. 329:647-652(1998) [PubMed: 9445394] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS PLD1A AND PLD1B).
[5]	"Molecular cloning of a cDNA homologous to a phospholipase D1 segment." Lassegue B., Alexander R.W., Griendling K. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-511.
[6]	"Phospholipase D not having transphosphatidylation reaction." Park S.K., Exton J.H. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PLD1C). Tissue: Lung.
[7]	"Requirements and effects of palmitoylation of rat PLD1." Xie Z., Ho W.T., Exton J.H. J. Biol. Chem. 276:9383-9391(2001) [PubMed: 11121416] [Abstract] Cited for: PALMITOYLATION AT CYS-240 AND CYS-241, MUTAGENESIS OF CYS-240 AND CYS-241, PHOSPHORYLATION.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AB003170 mRNA. Translation: BAA24076.1. AB003171 mRNA. Translation: BAA24077.1. U69550 mRNA. Translation: AAB86910.1. AB000778 mRNA. Translation: BAA24576.1. AB000779 mRNA. Translation: BAA24577.1. U88986 mRNA. Translation: AAB91329.1. AF017251 mRNA. Translation: AAD01609.1.
IPI	IPI00188898. IPI00231169. IPI00231170.
PIR	T13725. T13732. T13943. T46635.
RefSeq	NP_112254.1.
UniGene	Rn.11130
3D structure databases
SMR	P70496. Positions 97-211, 759-940.
ModBase	Search...
Protein-protein interaction databases
STRING	P70496.
PTM databases
PhosphoSite	P70496.
Genome annotation databases
Ensembl	ENSRNOT00000039296; ENSRNOP00000034466; ENSRNOG00000028156; Rattus norvegicus. [Genome view]
GeneID	25096.
KEGG	rno:25096.
UCSC	NM_030992. rat.
Organism-specific databases
CTD	25096.
RGD	3349. Pld1.
Phylogenomic databases
eggNOG	roNOG09371.
HOVERGEN	P70496.
InParanoid	P70496.
Enzyme and pathway databases
BRENDA	3.1.4.4. 248.
Gene expression databases
ArrayExpress	P70496.
Genevestigator	P70496.
GermOnline	ENSRNOG00000028156. Rattus norvegicus.
Family and domain databases
InterPro	IPR011993. PH_type. IPR015679. Phospholipase_D. IPR001849. Pleckstrin_homology. IPR001736. PLipase_D/transphosphatidylase. IPR016555. PLipase_D_euk. IPR001683. PX. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.30.1520.10. PX. 1 hit.
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00169. PH. 1 hit. PF00614. PLDc. 1 hit. PF00787. PX. 1 hit. [Graphical view]
PIRSF	PIRSF009376. Phospholipase_D_euk. 1 hit.
SMART	SM00233. PH. 1 hit. SM00155. PLDc. 2 hits. SM00312. PX. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50035. PLD. 2 hits. PS50195. PX. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	605397.

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Entry information

Entry name

PLD1_RAT

Accession

Primary (citable) accession number: P70496
Secondary accession number(s): O08959

Q9QWJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 18, 2001
Last modified:	January 19, 2010
This is version 85 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents