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Protein

Lactadherin

Gene

Mfge8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Contributes to phagocytic removal of apoptotic cells in many tissues. Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Appears to participate in the O-acetylation of GD3 ganglioside sialic acid.By similarity

GO - Biological processi

  • angiogenesis Source: UniProtKB-KW
  • cell adhesion Source: UniProtKB-KW
  • positive regulation of cell proliferation Source: RGD
  • response to estrogen Source: RGD
  • single fertilization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Angiogenesis, Cell adhesion, Fertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Lactadherin
Alternative name(s):
MFGM
Milk fat globule-EGF factor 8
Short name:
MFG-E8
O-acetyl GD3 ganglioside synthase
Short name:
AGS
SED1
Gene namesi
Name:Mfge8
Synonyms:Ags
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3083. Mfge8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 427405LactadherinPRO_0000007654Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 39By similarity
Disulfide bondi33 ↔ 49By similarity
Disulfide bondi51 ↔ 60By similarity
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence analysis
Disulfide bondi68 ↔ 79By similarity
Disulfide bondi73 ↔ 96By similarity
Disulfide bondi98 ↔ 107By similarity
Disulfide bondi111 ↔ 267By similarity
Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence analysis
Disulfide bondi254 ↔ 258By similarity
Disulfide bondi272 ↔ 427By similarity
Glycosylationi280 – 2801N-linked (GlcNAc...)Sequence analysis
Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP70490.

Expressioni

Tissue specificityi

Spleen, lung, heart, brain and muscle.

Gene expression databases

ExpressionAtlasiP70490. baseline and differential.
GenevisibleiP70490. RN.

Interactioni

Protein-protein interaction databases

IntActiP70490. 2 interactions.
MINTiMINT-4996971.

Structurei

3D structure databases

ProteinModelPortaliP70490.
SMRiP70490. Positions 272-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 6138EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini64 – 10845EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini111 – 267157F5/8 type C 1PROSITE-ProRule annotationAdd
BLAST
Domaini272 – 427156F5/8 type C 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi87 – 893Cell attachment site

Domaini

The F5/8 type C 2 domain mediates high-affinity binding to phosphatidylserine-containing membranes.By similarity

Sequence similaritiesi

Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 2 F5/8 type C domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00760000119073.
HOGENOMiHOG000236278.
HOVERGENiHBG002385.
InParanoidiP70490.
KOiK17253.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR027060. Lactadherin.
[Graphical view]
PANTHERiPTHR10127:SF303. PTHR10127:SF303. 1 hit.
PfamiPF00008. EGF. 2 hits.
PF00754. F5_F8_type_C. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00231. FA58C. 2 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFSRVLAAL CGVLLCASGL FAASGDFCDS SLCLNGGTCL MGQDNDIYCL
60 70 80 90 100
CPEGFTGLVC NETEKGPCSP NPCFHDAKCL VTEDTQRGDI FTEYICQCPV
110 120 130 140 150
GYSGIHCELG CSTKLGLEGG AIADSQISAS SVYMGFMGLQ RWGPELARLY
160 170 180 190 200
RTGIVNAWTA SSYDSKPWIQ VDFLRKMRVS GVMTQGASRA GRAEYLKTFK
210 220 230 240 250
VAYSLDGRRF EFIQDESGTG DKEFMGNQDN NSLKINMFNP TLEAQYIRLY
260 270 280 290 300
PVSCHRGCTL RFELLGCELH GCSEPLGLKN NTIPDSQITA SSSYKTWNLR
310 320 330 340 350
AFGWYPHLGR LDNQGKINAW TAQSNSAKEW LQVDLGTQKK VTGIITQGAR
360 370 380 390 400
DFGHIQYVAS YKVAHSDDGV QWTVYEEQGT SKVFQGNLDN NSHKKNIFEK
410 420
PFMARYVRVL PLSWHNRITL RLELLGC
Length:427
Mass (Da):47,413
Last modified:February 1, 1997 - v1
Checksum:iEA8C8631F3EE6047
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84068 mRNA. Translation: BAA12210.1.
BC085754 mRNA. Translation: AAH85754.1.
PIRiJC4915.
RefSeqiNP_001035276.1. NM_001040186.2.
NP_036943.1. NM_012811.3.
UniGeneiRn.3742.

Genome annotation databases

EnsembliENSRNOT00000024253; ENSRNOP00000024253; ENSRNOG00000017510.
GeneIDi25277.
KEGGirno:25277.
UCSCiRGD:3083. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84068 mRNA. Translation: BAA12210.1.
BC085754 mRNA. Translation: AAH85754.1.
PIRiJC4915.
RefSeqiNP_001035276.1. NM_001040186.2.
NP_036943.1. NM_012811.3.
UniGeneiRn.3742.

3D structure databases

ProteinModelPortaliP70490.
SMRiP70490. Positions 272-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP70490. 2 interactions.
MINTiMINT-4996971.

Proteomic databases

PRIDEiP70490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024253; ENSRNOP00000024253; ENSRNOG00000017510.
GeneIDi25277.
KEGGirno:25277.
UCSCiRGD:3083. rat.

Organism-specific databases

CTDi4240.
RGDi3083. Mfge8.

Phylogenomic databases

GeneTreeiENSGT00760000119073.
HOGENOMiHOG000236278.
HOVERGENiHBG002385.
InParanoidiP70490.
KOiK17253.

Miscellaneous databases

PROiP70490.

Gene expression databases

ExpressionAtlasiP70490. baseline and differential.
GenevisibleiP70490. RN.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR027060. Lactadherin.
[Graphical view]
PANTHERiPTHR10127:SF303. PTHR10127:SF303. 1 hit.
PfamiPF00008. EGF. 2 hits.
PF00754. F5_F8_type_C. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00231. FA58C. 2 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of cDNA for O-acetylation of GD3 ganglioside."
    Ogura K., Nara K., Watanabe Y., Kohno K., Tai T., Sanai Y.
    Biochem. Biophys. Res. Commun. 225:932-938(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiMFGM_RAT
AccessioniPrimary (citable) accession number: P70490
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 8, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.