Reviewed,
UniProtKB/Swiss-Prot P70478 (APC_RAT)
Last modified
November 25, 2008.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Adenomatous polyposis coli protein Short name=Protein APC | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 2842 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. APC activity is correlated with its phosphorylation state By similarity. |
| Subunit structure | Forms homooligomers and heterooligomers with APC2. Interacts with PDZ domains of DLG1 and DLG3. Associates with catenins. Binds axin. Interacts with the N-terminus of ARHGEF4, and the C-terminus of MAPRE1, MAPRE2 and MAPRE3. Found in a complex consisting of ARHGEF4, APC and CTNNB1. Interacts with DIAPH1 and DIAPH2. Interacts with APC2 By similarity. |
| Post-translational modification | Phosphorylated by GSK3B By similarity. |
| Sequence similarities | Belongs to the adenomatous polyposis coli (APC) family. Contains 7 ARM repeats. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 2842 | 2842 | Adenomatous polyposis coli protein | PRO_0000064629 | |||||
Regions | |||||||||
| Repeat | 451 – 493 | 43 | ARM 1 | ||||||
| Repeat | 503 – 545 | 43 | ARM 2 | ||||||
| Repeat | 546 – 589 | 44 | ARM 3 | ||||||
| Repeat | 590 – 636 | 47 | ARM 4 | ||||||
| Repeat | 637 – 681 | 45 | ARM 5 | ||||||
| Repeat | 682 – 723 | 42 | ARM 6 | ||||||
| Repeat | 724 – 765 | 42 | ARM 7 | ||||||
| Region | 1864 – 1891 | 28 | Highly charged | ||||||
| Coiled coil | 1 – 62 | 62 | Potential | ||||||
| Coiled coil | 125 – 260 | 136 | Potential | ||||||
| Motif | 2840 – 2842 | 3 | PDZ-binding By similarity | ||||||
| Compositional bias | 1 – 728 | 728 | Leu-rich | ||||||
| Compositional bias | 739 – 2831 | 2093 | Ser-rich | ||||||
| Compositional bias | 1130 – 1155 | 26 | Asp/Glu-rich (acidic) | ||||||
| Compositional bias | 1556 – 1575 | 20 | Asp/Glu-rich (acidic) | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1036 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1178 | 1 | Phosphoserine | ||||||
| Modified residue | 1368 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1695 | 1 | Phosphothreonine | ||||||
| Modified residue | 1714 | 1 | Phosphoserine | ||||||
| Modified residue | 1859 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1861 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1862 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 2125 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 2283 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 2398 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 2671 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 2674 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 2676 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 2710 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 2788 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 2834 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 2836 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 2837 | 1 | Phosphotyrosine By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 523 | 1 | C → R in an IQ-induced colon tumor | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "cDNA cloning of the rat APC gene and assignment to chromosome 18." Toyota M., Ushijima T., Kakiuchi H., Watanabe M., Imai K., Yachi A., Sugimura T., Nagao M. Mamm. Genome 6:746-748(1995) [PubMed: 8563176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer 344/N. Tissue: Brain. |
| [2] | "Specific 5'-GGGA-3'-->5'-GGA-3' mutation of the Apc gene in rat colon tumors induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine." Kakiuchi H., Watanabe M., Ushijima T., Toyota M., Imai K., Weisburger J.H., Sugimura T., Nagao M. Proc. Natl. Acad. Sci. U.S.A. 92:910-914(1995) [PubMed: 7846077] [Abstract] Cited for: MUTAGENESIS. Strain: Fischer 344/N and Sprague-Dawley. |
| [3] | "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed: 16641100] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1178; THR-1695 AND SER-1714, MASS SPECTROMETRY. Tissue: Kidney. |
Cross-references
Sequence databases | |
|---|---|
| D38629 mRNA. Translation: BAA07609.1. | |
| RefSeq | NP_036631.1. |
| UniGene | Rn.88057 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1M5I based on UniProtKB P25054. |
| SMR | P70478. Positions 2-55, 128-237. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P70478. |
PTM databases | |
| PhosphoSite | P70478. |
Genome annotation databases | |
| Ensembl | ENSRNOG00000020423. Rattus norvegicus. [Contig view] |
| GeneID | 24205. |
| KEGG | rno:24205. |
Organism-specific databases | |
| RGD | 2123. Apc. |
Phylogenomic databases | |
| HOVERGEN | P70478. |
Gene expression databases | |
| ArrayExpress | P70478. |
| GermOnline | ENSRNOG00000020423. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR009240. APC_15aa. IPR009234. APC_basic. IPR009223. APC_crr. IPR011989. ARM-like. IPR000225. Armadillo. IPR009232. EB1_bd. IPR009224. SAMP. [Graphical view] |
| Gene3D | G3DSA:1.25.10.10. ARM-like. 1 hit. |
| Pfam | PF05972. APC_15aa. 4 hits. PF05956. APC_basic. 1 hit. PF05923. APC_crr. 7 hits. PF00514. Arm. 4 hits. PF05937. EB1_binding. 1 hit. PF05924. SAMP. 3 hits. [Graphical view] |
| SMART | SM00185. ARM. 6 hits. [Graphical view] |
| PROSITE | PS50176. ARM_REPEAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 602593. |
Entry information
| Entry name | APC_RAT | ||||||||
| Accession | Primary (citable) accession number: P70478 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


