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Protein

Myelin transcription factor 1-like protein

Gene

Myt1l

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that plays a key role in neuronal differentiation (PubMed:9373037). Acts by specifically repressing expression of non-neuronal genes during neuron differentiation (By similarity). In contrast to other transcription repressors that inhibit specific lineages, mediates repression of multiple differentiation programs (By similarity). Also represses expression of negative regulators of neurogenesis, such as members of the Notch signaling pathway, including HES1 (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Directly binds the 5'-AAGTT-3' core motif present on the promoter of target genes and represses transcription by recruiting a multiprotein complex containing SIN3B (By similarity). The 5'-AAGTT-3' core motif is absent from the promoter of neural genes (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 65CCHHC-type 1PROSITE-ProRule annotationAdd BLAST44
Zinc fingeri496 – 539CCHHC-type 2PROSITE-ProRule annotationAdd BLAST44
Zinc fingeri540 – 583CCHHC-type 3PROSITE-ProRule annotationAdd BLAST44
Zinc fingeri895 – 938CCHHC-type 4PROSITE-ProRule annotationAdd BLAST44
Zinc fingeri944 – 987CCHHC-type 5PROSITE-ProRule annotationAdd BLAST44
Zinc fingeri997 – 1040CCHHC-type 6PROSITE-ProRule annotationAdd BLAST44

GO - Molecular functioni

  • cobalt ion binding Source: CAFA
  • retinoic acid-responsive element binding Source: RGD
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription coactivator activity Source: RGD
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding Source: NTNU_SB
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: InterPro
  • zinc ion binding Source: CAFA

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Myelin transcription factor 1-like protein
Short name:
MyT1-L
Short name:
MyT1L
Alternative name(s):
Neural zinc finger factor 11 Publication
Short name:
NZF-11 Publication
Gene namesi
Name:Myt1l
Synonyms:Nzf11 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi620550. Myt1l.

Subcellular locationi

  • Nucleus 1 Publication
  • Chromosome By similarity

  • Note: Preferentially binds to DNA binding sites that are in an open chromatin configuration.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000966751 – 1187Myelin transcription factor 1-like proteinAdd BLAST1187

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP70475.
PRIDEiP70475.

PTM databases

iPTMnetiP70475.
PhosphoSitePlusiP70475.

Expressioni

Tissue specificityi

Brain, testis and pituitary gland. Expression is higher in the brain than in the testis and pituitary gland. Highest level expression seen in the developing CNS.2 Publications

Developmental stagei

Detected at E11.5 and expression is seen throughout the proliferating cortex neuroepithelium, developing medulla, and spinal cord. At E12.5 found in the nasal epithelium and at E13.5 detected in the trigeminal ganglia, dorsal root ganglia and the ganglion cell layer of the retina. At E14-E15 expressed at high levels in the brain and spinal cord and then levels subsequently decrease.2 Publications

Interactioni

Subunit structurei

Interacts with SIN3B.By similarity

Protein-protein interaction databases

BioGridi250552. 1 interactor.
STRINGi10116.ENSRNOP00000005905.

Structurei

Secondary structure

11187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni515 – 517Combined sources3
Beta strandi524 – 528Combined sources5
Beta strandi534 – 539Combined sources6
Beta strandi542 – 544Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PXENMR-A487-548[»]
DisProtiDP00049.
ProteinModelPortaliP70475.
SMRiP70475.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70475.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1055 – 1131Sequence analysisAdd BLAST77

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi83 – 199Asp/Glu-richAdd BLAST117
Compositional biasi685 – 718Ser-richAdd BLAST34

Sequence similaritiesi

Belongs to the MYT1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 65CCHHC-type 1PROSITE-ProRule annotationAdd BLAST44
Zinc fingeri496 – 539CCHHC-type 2PROSITE-ProRule annotationAdd BLAST44
Zinc fingeri540 – 583CCHHC-type 3PROSITE-ProRule annotationAdd BLAST44
Zinc fingeri895 – 938CCHHC-type 4PROSITE-ProRule annotationAdd BLAST44
Zinc fingeri944 – 987CCHHC-type 5PROSITE-ProRule annotationAdd BLAST44
Zinc fingeri997 – 1040CCHHC-type 6PROSITE-ProRule annotationAdd BLAST44

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG3803. Eukaryota.
ENOG410ZPWS. LUCA.
HOGENOMiHOG000234099.
HOVERGENiHBG006433.
InParanoidiP70475.
OMAiPYYGKDP.
PhylomeDBiP70475.

Family and domain databases

InterProiView protein in InterPro
IPR013681. Myelin_TF.
IPR002515. Znf_C2HC.
PfamiView protein in Pfam
PF08474. MYT1. 1 hit.
PF01530. zf-C2HC. 6 hits.
SUPFAMiSSF103637. SSF103637. 6 hits.
PROSITEiView protein in PROSITE
PS51802. ZF_CCHHC. 6 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P70475-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDVDAEEKRH RTRSKGVRVP VEPAIQELFS CPTPGCDGSG HVSGKYARHR
60 70 80 90 100
SVYGCPLAKK RKTQDKQPQE PAPKRKPFAV KADSSSVDEC YESDGTEDMD
110 120 130 140 150
DKEEDDDEEF SEDNDEQGDD DDEDEVDRED EEEIEEEDDE DDEDDDDGDD
160 170 180 190 200
VEEEEDDDDE EEEEEEEEEE NEDHQMSCTR IMQDPEKDDN NNDEYDNYDE
210 220 230 240 250
LVAKSLLNLG KIAEDAAYRA RTESEMNSNT SNSLEDDSDK NENLGRKSEL
260 270 280 290 300
SLDLDSDVVR ETVDSLKLLA QGHGVVLSEN ISDRSYAEGM SQQDSRNMNY
310 320 330 340 350
VMLGKPMNNG LMEKMVEESD EEVCLSSLEC LRNQCFDLAR KLSETNPQDR
360 370 380 390 400
SQPPNMSVRQ HVRQEDDFPG RTPDRSYSDM MNLMRLEEQL SPRSRTFSSC
410 420 430 440 450
AKEDGCHERD DDTTSVNSDR SEEVFDMTKG NLTLLEKAIA LETERAKAMR
460 470 480 490 500
EKMAMDAGRR DNLRSYEDQS PRQLAGEDRK SKSSDSHVKK PYYDPSRTEK
510 520 530 540 550
RESKCPTPGC DGTGHVTGLY PHHRSLSGCP HKDRVPPEIL AMHENVLKCP
560 570 580 590 600
TPGCTGRGHV NSNRNSHRSL SGCPIAAAEK LAKAQEKHQS CDVSKSNQAS
610 620 630 640 650
DRVLRPMCFV KQLEIPQYGY RNNVPTTTPR SNLAKELEKY SKTSFEYNSY
660 670 680 690 700
DNHTYGKRAI APKVQTRDIS PKGYDDAKRY CKNASPSSST TSSYAPSSSS
710 720 730 740 750
NLSCGGGSSA SSTCSKSSFD YTHDMEAAHM AATAILNLST RCREMPQNLS
760 770 780 790 800
TKPQDLCTAR NPDMEVDENG TLDLSMNKQR PRDSCCPVLT PLEPMSPQQQ
810 820 830 840 850
AVMSSRCFQL SEGDCWDLPV DYTKMKPRRV DEEDPKEITP EDLDPFQEAL
860 870 880 890 900
EERRYPGEVT IPSPKPKYPQ CKESKKDLIT LSGCPLADKS IRSMLATSSQ
910 920 930 940 950
ELKCPTPGCD GSGHITGNYA SHRSLSGCPR AKKSGIRIAQ SKEDKEDQEP
960 970 980 990 1000
IRCPVPGCDG QGHITGKYAS HRSASGCPLA AKRQKDGYLN GSQFSWKSVK
1010 1020 1030 1040 1050
TEGMSCPTPG CDGSGHVSGS FLTHRSLSGC PRATSAMKKA KLSGEQMLTI
1060 1070 1080 1090 1100
KQRASNGIEN DEEIKQLDEE IKELNESNSQ MEADMIKLRT QVTITTMESN
1110 1120 1130 1140 1150
LKTIEEENKV IEQQNESLLH ELANLSQSLI HSLANIQLPH MDPINEQNFD
1160 1170 1180
AYVTTLTEMY TNQDRYQSPE NKALLENIKQ AVRGIQV
Length:1,187
Mass (Da):132,926
Last modified:June 7, 2017 - v3
Checksum:i10B2616415195DD1
GO
Isoform 2 (identifier: P70475-2)
Sequence is not available
Length:
Mass (Da):
Isoform 3 (identifier: P70475-3)
Sequence is not available
Length:
Mass (Da):

Sequence cautioni

The sequence AABR07063792 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AABR07063793 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AABR07063794 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AABR07063795 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AABR07063796 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AABR07063797 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAC52728 differs from that shown. Reason: Frameshift at positions 663 and 681.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39S → T in AAC52728 (PubMed:8631881).Curated1
Sequence conflicti226M → V in AAC52728 (PubMed:8631881).Curated1
Sequence conflicti237 – 239DSD → HSS in AAC52728 (PubMed:8631881).Curated3
Sequence conflicti415S → T in AAC52728 (PubMed:8631881).Curated1
Sequence conflicti517T → N in AAC52728 (PubMed:8631881).Curated1
Sequence conflicti561N → H in AAB40718 (PubMed:8980226).Curated1
Sequence conflicti804S → T in AAB40718 (PubMed:8980226).Curated1
Sequence conflicti918N → I in AAB40718 (PubMed:8980226).Curated1
Sequence conflicti1019G → A in AAB40718 (PubMed:8980226).Curated1
Sequence conflicti1092 – 1098VTITTME → ITTMD in AAB40718 (PubMed:8980226).Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48809 mRNA. Translation: AAC52728.1. Frameshift.
AABR07063792 Genomic DNA. No translation available.
AABR07063793 Genomic DNA. No translation available.
AABR07063794 Genomic DNA. No translation available.
AABR07063795 Genomic DNA. No translation available.
AABR07063796 Genomic DNA. No translation available.
AABR07063797 Genomic DNA. No translation available.
U67081 mRNA. Translation: AAB40718.1.
PIRiT46637.
RefSeqiNP_446340.1. NM_053888.1.
UniGeneiRn.10559.

Genome annotation databases

GeneIDi116668.
KEGGirno:116668.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMYT1L_RAT
AccessioniPrimary (citable) accession number: P70475
Secondary accession number(s): D4A9W2, P70589
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: June 7, 2017
Last modified: July 5, 2017
This is version 113 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families