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Protein

Acyl-protein thioesterase 1

Gene

Lypla1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Has low lysophospholipase activity (By similarity).By similarity3 Publications

Catalytic activityi

Palmitoyl-protein + H2O = palmitate + protein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei119Charge relay systemBy similarity1
Active sitei174Charge relay systemBy similarity1
Active sitei208Charge relay systemBy similarity1

GO - Molecular functioni

  • lysophospholipase activity Source: RGD
  • palmitoyl-(protein) hydrolase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processFatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-RNO-203615. eNOS activation.

Protein family/group databases

ESTHERiratno-lypla1a. LYsophospholipase_carboxylesterase.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-protein thioesterase 1 (EC:3.1.2.-)
Short name:
APT-1
Alternative name(s):
Lysophospholipase 1
Lysophospholipase I
Short name:
LPL-I
Short name:
LysoPLA I
Gene namesi
Name:Lypla1
Synonyms:Apt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi3025. Lypla1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2280.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022701 – 230Acyl-protein thioesterase 1Add BLAST230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP70470.
PRIDEiP70470.

PTM databases

iPTMnetiP70470.
PhosphoSitePlusiP70470.

Expressioni

Tissue specificityi

Ubiquitous. Detected at low levels in all tissues tested.2 Publications

Gene expression databases

BgeeiENSRNOG00000008320.
GenevisibleiP70470. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP70470. 1 interactor.
STRINGi10116.ENSRNOP00000011312.

Chemistry databases

BindingDBiP70470.

Structurei

3D structure databases

ProteinModelPortaliP70470.
SMRiP70470.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2112. Eukaryota.
COG0400. LUCA.
GeneTreeiENSGT00390000009648.
HOGENOMiHOG000260139.
HOVERGENiHBG052378.
InParanoidiP70470.
KOiK06128.
OMAiSACPEEM.
OrthoDBiEOG091G080L.
PhylomeDBiP70470.
TreeFamiTF314619.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiView protein in InterPro
IPR029058. AB_hydrolase.
IPR003140. PLipase/COase/thioEstase.
PfamiView protein in Pfam
PF02230. Abhydrolase_2. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P70470-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSSHIK
60 70 80 90 100
YICPHAPVMP VTLNMSMMMP SWFDIIGLSP DSQEDESGIK QAAETVKALI
110 120 130 140 150
DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA
160 170 180 190 200
SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK GLVNPANVTF
210 220 230
KVYEGMMHSS CQQEMMDVKY FIDKLLPPID
Length:230
Mass (Da):24,709
Last modified:February 1, 1997 - v1
Checksum:iAAFE8C4702EAAD74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63885 mRNA. Translation: BAA09935.1.
U97146 mRNA. Translation: AAC63430.1.
BC085750 mRNA. Translation: AAH85750.1.
RefSeqiNP_037138.1. NM_013006.1.
UniGeneiRn.3594.

Genome annotation databases

EnsembliENSRNOT00000011312; ENSRNOP00000011312; ENSRNOG00000008320.
GeneIDi25514.
KEGGirno:25514.
UCSCiRGD:3025. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLYPA1_RAT
AccessioniPrimary (citable) accession number: P70470
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: February 1, 1997
Last modified: June 7, 2017
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families