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Protein

Acyl-protein thioesterase 1

Gene

Lypla1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Has low lysophospholipase activity (By similarity).By similarity3 Publications

Catalytic activityi

Palmitoyl-protein + H2O = palmitate + protein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei119 – 1191Charge relay systemBy similarity
Active sitei174 – 1741Charge relay systemBy similarity
Active sitei208 – 2081Charge relay systemBy similarity

GO - Molecular functioni

  • lysophospholipase activity Source: RGD
  • palmitoyl-(protein) hydrolase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-RNO-203615. eNOS activation.

Protein family/group databases

ESTHERiratno-lypla1a. LYsophospholipase_carboxylesterase.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-protein thioesterase 1 (EC:3.1.2.-)
Short name:
APT-1
Alternative name(s):
Lysophospholipase 1
Lysophospholipase I
Short name:
LPL-I
Short name:
LysoPLA I
Gene namesi
Name:Lypla1
Synonyms:Apt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi3025. Lypla1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2280.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Acyl-protein thioesterase 1PRO_0000102270Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei224 – 2241N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP70470.
PRIDEiP70470.

PTM databases

iPTMnetiP70470.

Expressioni

Tissue specificityi

Ubiquitous. Detected at low levels in all tissues tested.2 Publications

Gene expression databases

GenevisibleiP70470. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP70470. 1 interaction.
STRINGi10116.ENSRNOP00000011312.

Chemistry

BindingDBiP70470.

Structurei

3D structure databases

ProteinModelPortaliP70470.
SMRiP70470. Positions 6-229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2112. Eukaryota.
COG0400. LUCA.
GeneTreeiENSGT00390000009648.
HOGENOMiHOG000260139.
HOVERGENiHBG052378.
InParanoidiP70470.
KOiK06128.
OMAiFSQGPIN.
OrthoDBiEOG7M0NSQ.
PhylomeDBiP70470.
TreeFamiTF314619.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR003140. PLipase/COase/thioEstase.
[Graphical view]
PfamiPF02230. Abhydrolase_2. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P70470-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSSHIK
60 70 80 90 100
YICPHAPVMP VTLNMSMMMP SWFDIIGLSP DSQEDESGIK QAAETVKALI
110 120 130 140 150
DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA
160 170 180 190 200
SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK GLVNPANVTF
210 220 230
KVYEGMMHSS CQQEMMDVKY FIDKLLPPID
Length:230
Mass (Da):24,709
Last modified:February 1, 1997 - v1
Checksum:iAAFE8C4702EAAD74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63885 mRNA. Translation: BAA09935.1.
U97146 mRNA. Translation: AAC63430.1.
BC085750 mRNA. Translation: AAH85750.1.
RefSeqiNP_037138.1. NM_013006.1.
UniGeneiRn.3594.

Genome annotation databases

EnsembliENSRNOT00000011312; ENSRNOP00000011312; ENSRNOG00000008320.
GeneIDi25514.
KEGGirno:25514.
UCSCiRGD:3025. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63885 mRNA. Translation: BAA09935.1.
U97146 mRNA. Translation: AAC63430.1.
BC085750 mRNA. Translation: AAH85750.1.
RefSeqiNP_037138.1. NM_013006.1.
UniGeneiRn.3594.

3D structure databases

ProteinModelPortaliP70470.
SMRiP70470. Positions 6-229.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP70470. 1 interaction.
STRINGi10116.ENSRNOP00000011312.

Chemistry

BindingDBiP70470.
ChEMBLiCHEMBL2280.

Protein family/group databases

ESTHERiratno-lypla1a. LYsophospholipase_carboxylesterase.

PTM databases

iPTMnetiP70470.

Proteomic databases

PaxDbiP70470.
PRIDEiP70470.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011312; ENSRNOP00000011312; ENSRNOG00000008320.
GeneIDi25514.
KEGGirno:25514.
UCSCiRGD:3025. rat.

Organism-specific databases

CTDi10434.
RGDi3025. Lypla1.

Phylogenomic databases

eggNOGiKOG2112. Eukaryota.
COG0400. LUCA.
GeneTreeiENSGT00390000009648.
HOGENOMiHOG000260139.
HOVERGENiHBG052378.
InParanoidiP70470.
KOiK06128.
OMAiFSQGPIN.
OrthoDBiEOG7M0NSQ.
PhylomeDBiP70470.
TreeFamiTF314619.

Enzyme and pathway databases

ReactomeiR-RNO-203615. eNOS activation.

Miscellaneous databases

PROiP70470.

Gene expression databases

GenevisibleiP70470. RN.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR003140. PLipase/COase/thioEstase.
[Graphical view]
PfamiPF02230. Abhydrolase_2. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Purification, cDNA cloning, and regulation of lysophospholipase from rat liver."
    Sugimoto H., Hayashi H., Yamashita S.
    J. Biol. Chem. 271:7705-7711(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-30 AND 192-196, FUNCTION, TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Liver.
  2. "cDNA cloning and expression of a novel family of enzymes with calcium-independent phospholipase A2 and lysophospholipase activities."
    Portilla D., Crew M.D., Grant D., Serrero G., Bates L.M., Dai G., Sasner M., Cheng J., Buonanno A.
    J. Am. Soc. Nephrol. 9:1178-1186(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Hypothalamus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  4. "A cytoplasmic acyl-protein thioesterase that removes palmitate from G protein alpha subunits and p21(RAS)."
    Duncan J.A., Gilman A.G.
    J. Biol. Chem. 273:15830-15837(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 98-112 AND 191-201, FUNCTION.

Entry informationi

Entry nameiLYPA1_RAT
AccessioniPrimary (citable) accession number: P70470
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: February 1, 1997
Last modified: June 8, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.