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P70459

- ERF_MOUSE

UniProt

P70459 - ERF_MOUSE

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Protein

ETS domain-containing transcription factor ERF

Gene

Erf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation (By similarity). Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment. May be important for regulating trophoblast stem cell differentiation.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi27 – 10781ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome
  3. transcription corepressor activity Source: Ensembl

GO - Biological processi

  1. chorio-allantoic fusion Source: MGI
  2. ectodermal cell differentiation Source: MGI
  3. in utero embryonic development Source: MGI
  4. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  5. regulation of transcription from RNA polymerase II promoter Source: RefGenome
  6. transcription from RNA polymerase II promoter Source: GOC
  7. trophoblast giant cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_188971. Oncogene Induced Senescence.

Names & Taxonomyi

Protein namesi
Recommended name:
ETS domain-containing transcription factor ERF
Gene namesi
Name:Erf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:109637. Erf.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice fail to undergo chorioallantoic attachment and labyrinth development and die in utero due to severe placenta defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 551551ETS domain-containing transcription factor ERFPRO_0000204102Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3 – 31PhosphothreonineBy similarity
Modified residuei7 – 71PhosphothreonineBy similarity
Modified residuei20 – 201PhosphoserineBy similarity
Modified residuei185 – 1851PhosphoserineBy similarity
Modified residuei190 – 1901PhosphoserineBy similarity
Modified residuei327 – 3271PhosphoserineBy similarity
Modified residuei433 – 4331PhosphoserineBy similarity
Modified residuei437 – 4371PhosphoserineBy similarity
Modified residuei443 – 4431PhosphothreonineBy similarity
Modified residuei446 – 4461PhosphoserineBy similarity
Modified residuei529 – 5291Phosphothreonine; by MAPK1By similarity
Modified residuei534 – 5341PhosphoserineBy similarity
Modified residuei551 – 5511PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-529. Phosphorylation regulates the activity of ERF (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP70459.
PaxDbiP70459.
PRIDEiP70459.

PTM databases

PhosphoSiteiP70459.

Expressioni

Tissue specificityi

Expressed along the osteogenic margins of the developing calvarial bones, in a similar distribution to that observed for the master osteogenic regulator RUNX2.1 Publication

Developmental stagei

Expressed throughout embryonic development and adulthood. In the developing placenta, after 7.5 dpc expression is restricted to the extraembryonic ectoderm, and after 9.5 dpc to subpopulation of labyrinth cells.1 Publication

Gene expression databases

BgeeiP70459.
CleanExiMM_ERF.
ExpressionAtlasiP70459. baseline and differential.
GenevestigatoriP70459.

Structurei

3D structure databases

ProteinModelPortaliP70459.
SMRiP70459. Positions 24-111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi166 – 1716Poly-Ser
Compositional biasi290 – 2934Poly-Gly
Compositional biasi362 – 37312Poly-SerAdd
BLAST
Compositional biasi420 – 4256Poly-Pro

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG331846.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000070246.
HOVERGENiHBG005183.
InParanoidiP70459.
KOiK09434.
OrthoDBiEOG71P2BB.
PhylomeDBiP70459.
TreeFamiTF351065.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70459-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTPADTGFA FPDWAYKPES SPGSRQIQLW HFILELLRKE EYQGVIAWQG
60 70 80 90 100
DYGEFVIKDP DEVARLWGVR KCKPQMNYDK LSRALRYYYN KRILHKTKGK
110 120 130 140 150
RFTYKFNFNK LVLVNYPFID MGLAGGAVPQ SAPPVPSGGS HFRFPPSTPS
160 170 180 190 200
EVLSPTEDPR SPPACSSSSS SLFSAVVARR LGRGSVSDCS DGTSELEEPL
210 220 230 240 250
GEDPRARPPG PPELGAFRGP PLARLPHDPG VFRVYPRPRG GPEPLSPFPV
260 270 280 290 300
SPLAGPGSLL PPQLSPALPM TPTHLAYTPS PTLSPMYPSG GGGPSGSGGG
310 320 330 340 350
SHFSFSPEDM KRYLQAHTQS VYNYHLSPRA FLHYPGLVVP QPQRPDKCPL
360 370 380 390 400
PPMAPETPPV PSSASSSSSS SSSPFKFKLQ PPPLGRRQRA AGEKAPGGTD
410 420 430 440 450
KSSGGSGSGG LAEGAGAVAP PPPPPQIKVE PISEGESEEV EVTDISDEDE
460 470 480 490 500
EDGEVFKTPR APPAPPKPEP GEAPGVAQCM PLKLRFKRRW SEDCRLEGGG
510 520 530 540 550
CLSGGPEDEG EDKKVRGDVG PGESGGPLTP RRVSSDLQHA TAQLSLEHRD

S
Length:551
Mass (Da):59,050
Last modified:February 1, 1997 - v1
Checksum:i5AC1B72FB2743FE5
GO

Sequence cautioni

The sequence AAH53045.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH59176.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681G → V in AAH63092. (PubMed:15489334)Curated
Sequence conflicti134 – 1341P → S in AAH59176. (PubMed:15489334)Curated
Sequence conflicti134 – 1341P → S in AAH53045. (PubMed:15489334)Curated
Sequence conflicti396 – 3961P → L in BAE34912. (PubMed:16141072)Curated
Sequence conflicti396 – 3961P → L in EDL24284. 1 PublicationCurated
Sequence conflicti396 – 3961P → L in AAH59176. (PubMed:15489334)Curated
Sequence conflicti396 – 3961P → L in AAH53045. (PubMed:15489334)Curated
Sequence conflicti396 – 3961P → L in AAH63092. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58533, U58534 Genomic DNA. Translation: AAC09474.1.
AK159225 mRNA. Translation: BAE34912.1.
CH466593 Genomic DNA. Translation: EDL24284.1.
BC053045 mRNA. Translation: AAH53045.1. Different initiation.
BC059176 mRNA. Translation: AAH59176.1. Different initiation.
BC063092 mRNA. Translation: AAH63092.1.
CCDSiCCDS20978.1.
RefSeqiNP_034285.3. NM_010155.3.
UniGeneiMm.8068.

Genome annotation databases

EnsembliENSMUST00000045847; ENSMUSP00000041912; ENSMUSG00000040857.
GeneIDi13875.
KEGGimmu:13875.
UCSCiuc009frz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58533 , U58534 Genomic DNA. Translation: AAC09474.1 .
AK159225 mRNA. Translation: BAE34912.1 .
CH466593 Genomic DNA. Translation: EDL24284.1 .
BC053045 mRNA. Translation: AAH53045.1 . Different initiation.
BC059176 mRNA. Translation: AAH59176.1 . Different initiation.
BC063092 mRNA. Translation: AAH63092.1 .
CCDSi CCDS20978.1.
RefSeqi NP_034285.3. NM_010155.3.
UniGenei Mm.8068.

3D structure databases

ProteinModelPortali P70459.
SMRi P70459. Positions 24-111.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P70459.

Proteomic databases

MaxQBi P70459.
PaxDbi P70459.
PRIDEi P70459.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000045847 ; ENSMUSP00000041912 ; ENSMUSG00000040857 .
GeneIDi 13875.
KEGGi mmu:13875.
UCSCi uc009frz.2. mouse.

Organism-specific databases

CTDi 2077.
MGIi MGI:109637. Erf.

Phylogenomic databases

eggNOGi NOG331846.
GeneTreei ENSGT00760000118907.
HOGENOMi HOG000070246.
HOVERGENi HBG005183.
InParanoidi P70459.
KOi K09434.
OrthoDBi EOG71P2BB.
PhylomeDBi P70459.
TreeFami TF351065.

Enzyme and pathway databases

Reactomei REACT_188971. Oncogene Induced Senescence.

Miscellaneous databases

ChiTaRSi Erf. mouse.
NextBioi 284800.
PROi P70459.
SOURCEi Search...

Gene expression databases

Bgeei P70459.
CleanExi MM_ERF.
ExpressionAtlasi P70459. baseline and differential.
Genevestigatori P70459.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00178. Ets. 1 hit.
[Graphical view ]
PRINTSi PR00454. ETSDOMAIN.
SMARTi SM00413. ETS. 1 hit.
[Graphical view ]
PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "ERF: genomic organization, chromosomal localization and promoter analysis of the human and mouse genes."
    Liu D., Pavlopoulos E., Modi W., Moschonas N., Mavrothalassitis G.J.
    Oncogene 14:1445-1451(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Jaw.
  5. "Transcriptional repressor erf determines extraembryonic ectoderm differentiation."
    Papadaki C., Alexiou M., Cecena G., Verykokakis M., Bilitou A., Cross J.C., Oshima R.G., Mavrothalassitis G.
    Mol. Cell. Biol. 27:5201-5213(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  6. Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiERF_MOUSE
AccessioniPrimary (citable) accession number: P70459
Secondary accession number(s): Q3TXK4, Q6P544, Q7TSJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: November 26, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3