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Protein

ETS domain-containing transcription factor ERF

Gene

Erf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation (By similarity). Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment. May be important for regulating trophoblast stem cell differentiation.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi27 – 107ETSPROSITE-ProRule annotationAdd BLAST81

GO - Molecular functioni

GO - Biological processi

  • chorio-allantoic fusion Source: MGI
  • ectodermal cell differentiation Source: MGI
  • in utero embryonic development Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW
  • trophoblast giant cell differentiation Source: MGI

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
ETS domain-containing transcription factor ERF
Gene namesi
Name:Erf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:109637. Erf.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice fail to undergo chorioallantoic attachment and labyrinth development and die in utero due to severe placenta defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041021 – 551ETS domain-containing transcription factor ERFAdd BLAST551

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3PhosphothreonineBy similarity1
Modified residuei7PhosphothreonineBy similarity1
Modified residuei20PhosphoserineBy similarity1
Modified residuei24PhosphoserineBy similarity1
Modified residuei185PhosphoserineBy similarity1
Modified residuei190PhosphoserineBy similarity1
Modified residuei327PhosphoserineBy similarity1
Modified residuei433PhosphoserineBy similarity1
Modified residuei437PhosphoserineBy similarity1
Modified residuei443PhosphothreonineBy similarity1
Modified residuei446PhosphoserineBy similarity1
Cross-linki467Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki483Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki514Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei529Phosphothreonine; by MAPK1By similarity1
Modified residuei534PhosphoserineBy similarity1
Modified residuei535PhosphoserineBy similarity1
Modified residuei551PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-529. Phosphorylation regulates the activity of ERF (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP70459.
PaxDbiP70459.
PeptideAtlasiP70459.
PRIDEiP70459.

PTM databases

iPTMnetiP70459.
PhosphoSitePlusiP70459.

Expressioni

Tissue specificityi

Expressed along the osteogenic margins of the developing calvarial bones, in a similar distribution to that observed for the master osteogenic regulator RUNX2.1 Publication

Developmental stagei

Expressed throughout embryonic development and adulthood. In the developing placenta, after 7.5 dpc expression is restricted to the extraembryonic ectoderm, and after 9.5 dpc to subpopulation of labyrinth cells.1 Publication

Gene expression databases

CleanExiMM_ERF.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041912.

Structurei

3D structure databases

ProteinModelPortaliP70459.
SMRiP70459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi166 – 171Poly-Ser6
Compositional biasi290 – 293Poly-Gly4
Compositional biasi362 – 373Poly-SerAdd BLAST12
Compositional biasi420 – 425Poly-Pro6

Sequence similaritiesi

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
HOGENOMiHOG000070246.
HOVERGENiHBG005183.
InParanoidiP70459.
KOiK09434.
PhylomeDBiP70459.
TreeFamiTF351065.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR032925. ERF.
IPR000418. Ets_dom.
IPR036388. WH-like_DNA-bd_sf.
IPR036390. WH_DNA-bd_sf.
PANTHERiPTHR11849:SF31. PTHR11849:SF31. 2 hits.
PfamiView protein in Pfam
PF00178. Ets. 1 hit.
PRINTSiPR00454. ETSDOMAIN.
SMARTiView protein in SMART
SM00413. ETS. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiView protein in PROSITE
PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P70459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTPADTGFA FPDWAYKPES SPGSRQIQLW HFILELLRKE EYQGVIAWQG
60 70 80 90 100
DYGEFVIKDP DEVARLWGVR KCKPQMNYDK LSRALRYYYN KRILHKTKGK
110 120 130 140 150
RFTYKFNFNK LVLVNYPFID MGLAGGAVPQ SAPPVPSGGS HFRFPPSTPS
160 170 180 190 200
EVLSPTEDPR SPPACSSSSS SLFSAVVARR LGRGSVSDCS DGTSELEEPL
210 220 230 240 250
GEDPRARPPG PPELGAFRGP PLARLPHDPG VFRVYPRPRG GPEPLSPFPV
260 270 280 290 300
SPLAGPGSLL PPQLSPALPM TPTHLAYTPS PTLSPMYPSG GGGPSGSGGG
310 320 330 340 350
SHFSFSPEDM KRYLQAHTQS VYNYHLSPRA FLHYPGLVVP QPQRPDKCPL
360 370 380 390 400
PPMAPETPPV PSSASSSSSS SSSPFKFKLQ PPPLGRRQRA AGEKAPGGTD
410 420 430 440 450
KSSGGSGSGG LAEGAGAVAP PPPPPQIKVE PISEGESEEV EVTDISDEDE
460 470 480 490 500
EDGEVFKTPR APPAPPKPEP GEAPGVAQCM PLKLRFKRRW SEDCRLEGGG
510 520 530 540 550
CLSGGPEDEG EDKKVRGDVG PGESGGPLTP RRVSSDLQHA TAQLSLEHRD

S
Length:551
Mass (Da):59,050
Last modified:February 1, 1997 - v1
Checksum:i5AC1B72FB2743FE5
GO

Sequence cautioni

P70459: The sequence AAH53045 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
P70459: The sequence AAH59176 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68G → V in AAH63092 (PubMed:15489334).Curated1
Sequence conflicti134P → S in AAH59176 (PubMed:15489334).Curated1
Sequence conflicti134P → S in AAH53045 (PubMed:15489334).Curated1
Sequence conflicti396P → L in BAE34912 (PubMed:16141072).Curated1
Sequence conflicti396P → L in EDL24284 (Ref. 3) Curated1
Sequence conflicti396P → L in AAH59176 (PubMed:15489334).Curated1
Sequence conflicti396P → L in AAH53045 (PubMed:15489334).Curated1
Sequence conflicti396P → L in AAH63092 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58533, U58534 Genomic DNA. Translation: AAC09474.1.
AK159225 mRNA. Translation: BAE34912.1.
CH466593 Genomic DNA. Translation: EDL24284.1.
BC053045 mRNA. Translation: AAH53045.1. Different initiation.
BC059176 mRNA. Translation: AAH59176.1. Different initiation.
BC063092 mRNA. Translation: AAH63092.1.
CCDSiCCDS20978.1.
RefSeqiNP_034285.3. NM_010155.3.
UniGeneiMm.8068.

Genome annotation databases

GeneIDi13875.
KEGGimmu:13875.
UCSCiuc009frz.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiERF_MOUSE
AccessioniPrimary (citable) accession number: P70459
Secondary accession number(s): Q3TXK4, Q6P544, Q7TSJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: October 25, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families