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P70459

- ERF_MOUSE

UniProt

P70459 - ERF_MOUSE

Protein

ETS domain-containing transcription factor ERF

Gene

Erf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation By similarity. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment. May be important for regulating trophoblast stem cell differentiation.By similarity1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi27 – 10781ETSPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome
    3. transcription corepressor activity Source: Ensembl

    GO - Biological processi

    1. chorio-allantoic fusion Source: MGI
    2. ectodermal cell differentiation Source: MGI
    3. in utero embryonic development Source: MGI
    4. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    5. regulation of transcription from RNA polymerase II promoter Source: RefGenome
    6. transcription from RNA polymerase II promoter Source: GOC
    7. trophoblast giant cell differentiation Source: MGI

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_188971. Oncogene Induced Senescence.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ETS domain-containing transcription factor ERF
    Gene namesi
    Name:Erf
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:109637. Erf.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice fail to undergo chorioallantoic attachment and labyrinth development and die in utero due to severe placenta defects.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 551551ETS domain-containing transcription factor ERFPRO_0000204102Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei3 – 31PhosphothreonineBy similarity
    Modified residuei7 – 71PhosphothreonineBy similarity
    Modified residuei20 – 201PhosphoserineBy similarity
    Modified residuei185 – 1851PhosphoserineBy similarity
    Modified residuei190 – 1901PhosphoserineBy similarity
    Modified residuei327 – 3271PhosphoserineBy similarity
    Modified residuei433 – 4331PhosphoserineBy similarity
    Modified residuei437 – 4371PhosphoserineBy similarity
    Modified residuei443 – 4431PhosphothreonineBy similarity
    Modified residuei446 – 4461PhosphoserineBy similarity
    Modified residuei529 – 5291Phosphothreonine; by MAPK1By similarity
    Modified residuei534 – 5341PhosphoserineBy similarity
    Modified residuei551 – 5511PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-529. Phosphorylation regulates the activity of ERF By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP70459.
    PRIDEiP70459.

    PTM databases

    PhosphoSiteiP70459.

    Expressioni

    Tissue specificityi

    Expressed along the osteogenic margins of the developing calvarial bones, in a similar distribution to that observed for the master osteogenic regulator RUNX2.1 Publication

    Developmental stagei

    Expressed throughout embryonic development and adulthood. In the developing placenta, after 7.5 dpc expression is restricted to the extraembryonic ectoderm, and after 9.5 dpc to subpopulation of labyrinth cells.1 Publication

    Gene expression databases

    ArrayExpressiP70459.
    BgeeiP70459.
    CleanExiMM_ERF.
    GenevestigatoriP70459.

    Structurei

    3D structure databases

    ProteinModelPortaliP70459.
    SMRiP70459. Positions 24-111.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi166 – 1716Poly-Ser
    Compositional biasi290 – 2934Poly-Gly
    Compositional biasi362 – 37312Poly-SerAdd
    BLAST
    Compositional biasi420 – 4256Poly-Pro

    Sequence similaritiesi

    Belongs to the ETS family.Curated
    Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG331846.
    GeneTreeiENSGT00750000117307.
    HOGENOMiHOG000070246.
    HOVERGENiHBG005183.
    InParanoidiQ6P544.
    KOiK09434.
    OrthoDBiEOG71P2BB.
    PhylomeDBiP70459.
    TreeFamiTF351065.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR000418. Ets_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00178. Ets. 1 hit.
    [Graphical view]
    PRINTSiPR00454. ETSDOMAIN.
    SMARTiSM00413. ETS. 1 hit.
    [Graphical view]
    PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P70459-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTPADTGFA FPDWAYKPES SPGSRQIQLW HFILELLRKE EYQGVIAWQG    50
    DYGEFVIKDP DEVARLWGVR KCKPQMNYDK LSRALRYYYN KRILHKTKGK 100
    RFTYKFNFNK LVLVNYPFID MGLAGGAVPQ SAPPVPSGGS HFRFPPSTPS 150
    EVLSPTEDPR SPPACSSSSS SLFSAVVARR LGRGSVSDCS DGTSELEEPL 200
    GEDPRARPPG PPELGAFRGP PLARLPHDPG VFRVYPRPRG GPEPLSPFPV 250
    SPLAGPGSLL PPQLSPALPM TPTHLAYTPS PTLSPMYPSG GGGPSGSGGG 300
    SHFSFSPEDM KRYLQAHTQS VYNYHLSPRA FLHYPGLVVP QPQRPDKCPL 350
    PPMAPETPPV PSSASSSSSS SSSPFKFKLQ PPPLGRRQRA AGEKAPGGTD 400
    KSSGGSGSGG LAEGAGAVAP PPPPPQIKVE PISEGESEEV EVTDISDEDE 450
    EDGEVFKTPR APPAPPKPEP GEAPGVAQCM PLKLRFKRRW SEDCRLEGGG 500
    CLSGGPEDEG EDKKVRGDVG PGESGGPLTP RRVSSDLQHA TAQLSLEHRD 550
    S 551
    Length:551
    Mass (Da):59,050
    Last modified:February 1, 1997 - v1
    Checksum:i5AC1B72FB2743FE5
    GO

    Sequence cautioni

    The sequence AAH53045.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence AAH59176.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 681G → V in AAH63092. (PubMed:15489334)Curated
    Sequence conflicti134 – 1341P → S in AAH59176. (PubMed:15489334)Curated
    Sequence conflicti134 – 1341P → S in AAH53045. (PubMed:15489334)Curated
    Sequence conflicti396 – 3961P → L in BAE34912. (PubMed:16141072)Curated
    Sequence conflicti396 – 3961P → L in EDL24284. 1 PublicationCurated
    Sequence conflicti396 – 3961P → L in AAH59176. (PubMed:15489334)Curated
    Sequence conflicti396 – 3961P → L in AAH53045. (PubMed:15489334)Curated
    Sequence conflicti396 – 3961P → L in AAH63092. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U58533, U58534 Genomic DNA. Translation: AAC09474.1.
    AK159225 mRNA. Translation: BAE34912.1.
    CH466593 Genomic DNA. Translation: EDL24284.1.
    BC053045 mRNA. Translation: AAH53045.1. Different initiation.
    BC059176 mRNA. Translation: AAH59176.1. Different initiation.
    BC063092 mRNA. Translation: AAH63092.1.
    CCDSiCCDS20978.1.
    RefSeqiNP_034285.3. NM_010155.3.
    UniGeneiMm.8068.

    Genome annotation databases

    EnsembliENSMUST00000045847; ENSMUSP00000041912; ENSMUSG00000040857.
    GeneIDi13875.
    KEGGimmu:13875.
    UCSCiuc009frz.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U58533 , U58534 Genomic DNA. Translation: AAC09474.1 .
    AK159225 mRNA. Translation: BAE34912.1 .
    CH466593 Genomic DNA. Translation: EDL24284.1 .
    BC053045 mRNA. Translation: AAH53045.1 . Different initiation.
    BC059176 mRNA. Translation: AAH59176.1 . Different initiation.
    BC063092 mRNA. Translation: AAH63092.1 .
    CCDSi CCDS20978.1.
    RefSeqi NP_034285.3. NM_010155.3.
    UniGenei Mm.8068.

    3D structure databases

    ProteinModelPortali P70459.
    SMRi P70459. Positions 24-111.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P70459.

    Proteomic databases

    PaxDbi P70459.
    PRIDEi P70459.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000045847 ; ENSMUSP00000041912 ; ENSMUSG00000040857 .
    GeneIDi 13875.
    KEGGi mmu:13875.
    UCSCi uc009frz.2. mouse.

    Organism-specific databases

    CTDi 2077.
    MGIi MGI:109637. Erf.

    Phylogenomic databases

    eggNOGi NOG331846.
    GeneTreei ENSGT00750000117307.
    HOGENOMi HOG000070246.
    HOVERGENi HBG005183.
    InParanoidi Q6P544.
    KOi K09434.
    OrthoDBi EOG71P2BB.
    PhylomeDBi P70459.
    TreeFami TF351065.

    Enzyme and pathway databases

    Reactomei REACT_188971. Oncogene Induced Senescence.

    Miscellaneous databases

    ChiTaRSi ERF. mouse.
    NextBioi 284800.
    PROi P70459.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70459.
    Bgeei P70459.
    CleanExi MM_ERF.
    Genevestigatori P70459.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR000418. Ets_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00178. Ets. 1 hit.
    [Graphical view ]
    PRINTSi PR00454. ETSDOMAIN.
    SMARTi SM00413. ETS. 1 hit.
    [Graphical view ]
    PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ERF: genomic organization, chromosomal localization and promoter analysis of the human and mouse genes."
      Liu D., Pavlopoulos E., Modi W., Moschonas N., Mavrothalassitis G.J.
      Oncogene 14:1445-1451(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain and Jaw.
    5. "Transcriptional repressor erf determines extraembryonic ectoderm differentiation."
      Papadaki C., Alexiou M., Cecena G., Verykokakis M., Bilitou A., Cross J.C., Oshima R.G., Mavrothalassitis G.
      Mol. Cell. Biol. 27:5201-5213(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    6. Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiERF_MOUSE
    AccessioniPrimary (citable) accession number: P70459
    Secondary accession number(s): Q3TXK4, Q6P544, Q7TSJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3