P70453 (PDE7A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A
EC=3.1.4.17

Alternative name(s):
P2A

Gene names

Name:

Pde7a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction By similarity.
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Enzyme regulation	Insensitive to all selective PDE inhibitors.
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Subunit structure	Interacts with CBFA2T3 By similarity.
Tissue specificity	Widely expressed with highest levels in the skeletal muscle.
Domain	Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding cAMP
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cAMP catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway signal transduction Inferred from electronic annotation. Source: InterPro
Molecular_function	3',5'-cyclic-AMP phosphodiesterase activity Inferred from sequence orthology Ref.1. Source: MGI metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P70453-1)

Also known as: PDE7A2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P70453-2)

Also known as: PDE7A1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MGITLIWCLALVLIKWITSK → MEVCYQLPVLPLDRPVPQHVLSRRGAISFSSSSALFGCPHPRQLSQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A		PRO_0000198834
Regions
	Region	161 – 425	265	Catalytic By similarity
Sites
	Active site	186	1	Proton donor By similarity
	Metal binding	190	1	Divalent metal cation 1 By similarity
	Metal binding	226	1	Divalent metal cation 1 By similarity
	Metal binding	227	1	Divalent metal cation 1 By similarity
	Metal binding	227	1	Divalent metal cation 2 By similarity
	Metal binding	336	1	Divalent metal cation 1 By similarity
Natural variations
	Alternative sequence	1 – 20	20	MGITL…WITSK → MEVCYQLPVLPLDRPVPQHV LSRRGAISFSSSSALFGCPH PRQLSQ in isoform 2.		VSP_004594
Experimental info
	Sequence conflict	407	1	D → A in AAB08479. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PDE7A2) [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 0B84B62094BD9F6E	FASTA	456	52,486
	10 20 30 40 50 60 MGITLIWCLA LVLIKWITSK RRGAISYDSS DQTALYIRML GDVRVRSRAG FETERRGSHP 70 80 90 100 110 120 YIDFRIFHSQ SDIEASVSAR NIRRLLSFQR YLRSSRVFRG ATVCSSLDIL DEDYNGQAKC 130 140 150 160 170 180 MLEKVGNWNF DIFLFDRLTN GNSLVSLTFH LFSLHGLIEY FHLDMVKLRR FLVMIQEDYH 190 200 210 220 230 240 SQNPYHNAVH AADVTQAMHC YLKEPKLASS VTPWDILLSL IAAATHDLDH PGVNQPFLIK 250 260 270 280 290 300 TNHYLATLYK NSSVLENHHW RSAVGLLRES GLFSHLPLES RQEMEAQIGA LILATDISRQ 310 320 330 340 350 360 NEYLSLFRSH LDKGDLHLDD GRHRHLVLQM ALKCADICNP CRNWELSKQW SEKVTEEFFH 370 380 390 400 410 420 QGDIEKKYHL GVSPLCDRQT ESIANIQIGF MTYLVEPLFT EWARFSDTRL SQTMLGHVGL 430 440 450 NKASWKGLQR QQPSSEDANA AFELNSQLLT QENRLS
	Isoform 2 (PDE7A1) [UniParc]. Checksum: E8470DD85BFF7714	FASTA	482	55,289
	10 20 30 40 50 60 MEVCYQLPVL PLDRPVPQHV LSRRGAISFS SSSALFGCPH PRQLSQRRGA ISYDSSDQTA 70 80 90 100 110 120 LYIRMLGDVR VRSRAGFETE RRGSHPYIDF RIFHSQSDIE ASVSARNIRR LLSFQRYLRS 130 140 150 160 170 180 SRVFRGATVC SSLDILDEDY NGQAKCMLEK VGNWNFDIFL FDRLTNGNSL VSLTFHLFSL 190 200 210 220 230 240 HGLIEYFHLD MVKLRRFLVM IQEDYHSQNP YHNAVHAADV TQAMHCYLKE PKLASSVTPW 250 260 270 280 290 300 DILLSLIAAA THDLDHPGVN QPFLIKTNHY LATLYKNSSV LENHHWRSAV GLLRESGLFS 310 320 330 340 350 360 HLPLESRQEM EAQIGALILA TDISRQNEYL SLFRSHLDKG DLHLDDGRHR HLVLQMALKC 370 380 390 400 410 420 ADICNPCRNW ELSKQWSEKV TEEFFHQGDI EKKYHLGVSP LCDRQTESIA NIQIGFMTYL 430 440 450 460 470 480 VEPLFTEWAR FSDTRLSQTM LGHVGLNKAS WKGLQRQQPS SEDANAAFEL NSQLLTQENR LS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants." Bloom T.J., Beavo J.A. Proc. Natl. Acad. Sci. U.S.A. 93:14188-14192(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Skeletal muscle.
[2]	"Cloning, characterization, and tissue distribution of mouse phosphodiesterase 7A1." Wang P., Wu P., Egan R.W., Billah M.M. Biochem. Biophys. Res. Commun. 276:1271-1277(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain and Testis.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Amnion.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U68171 mRNA. Translation: AAB08479.1. AY007702 mRNA. Translation: AAG16295.1. AK168957 mRNA. Translation: BAE40763.1.
IPI	IPI00230552. IPI00762046.
RefSeq	NP_001116231.1. NM_001122759.1. NP_032828.2. NM_008802.2.
UniGene	Mm.355614.
3D structure databases
ProteinModelPortal	P70453.
SMR	P70453. Positions 113-430.
ModBase	Search...
Protein-protein interaction databases
IntAct	P70453. 1 interaction.
PTM databases
PhosphoSite	P70453.
Proteomic databases
PRIDE	P70453.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000091314; ENSMUSP00000088863; ENSMUSG00000069094. ENSMUST00000099195; ENSMUSP00000096800; ENSMUSG00000069094.
GeneID	18583.
KEGG	mmu:18583.
UCSC	uc008ort.2. mouse.
Organism-specific databases
CTD	5150.
MGI	MGI:1202402. Pde7a.
Phylogenomic databases
eggNOG	NOG300643.
GeneTree	ENSGT00690000101749.
HOGENOM	HOG000220881.
HOVERGEN	HBG053543.
KO	K01120.
OMA	SHMPLES.
Enzyme and pathway databases
UniPathway	UPA00762; UER00747.
Gene expression databases
ArrayExpress	P70453.
Bgee	P70453.
CleanEx	MM_PDE7A.
Genevestigator	P70453.
GermOnline	ENSMUSG00000069094. Mus musculus.
Family and domain databases
Gene3D	1.10.1300.10. 1 hit.
InterPro	IPR003607. HD/PDEase_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	294452.
SOURCE	Search...

Entry information

Entry name

PDE7A_MOUSE

Accession

Primary (citable) accession number: P70453
Secondary accession number(s): Q3TFY5, Q9ERB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 27, 2011
Last modified:	May 29, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program