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P70453

- PDE7A_MOUSE

UniProt

P70453 - PDE7A_MOUSE

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Protein
High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A
Gene
Pde7a
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction By similarity.

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Enzyme regulationi

Insensitive to all selective PDE inhibitors.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei186 – 1861Proton donor By similarity
Metal bindingi190 – 1901Divalent metal cation 1 By similarity
Metal bindingi226 – 2261Divalent metal cation 1 By similarity
Metal bindingi227 – 2271Divalent metal cation 1 By similarity
Metal bindingi227 – 2271Divalent metal cation 2 By similarity
Metal bindingi336 – 3361Divalent metal cation 1 By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: MGI
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cAMP catabolic process Source: UniProtKB-UniPathway
  2. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00762; UER00747.

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A (EC:3.1.4.53)
Alternative name(s):
P2A
Gene namesi
Name:Pde7a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1202402. Pde7a.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A
PRO_0000198834Add
BLAST

Proteomic databases

PRIDEiP70453.

PTM databases

PhosphoSiteiP70453.

Expressioni

Tissue specificityi

Widely expressed with highest levels in the skeletal muscle.

Gene expression databases

ArrayExpressiP70453.
BgeeiP70453.
CleanExiMM_PDE7A.
GenevestigatoriP70453.

Interactioni

Subunit structurei

Interacts with CBFA2T3 By similarity.

Protein-protein interaction databases

BioGridi202081. 1 interaction.
IntActiP70453. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP70453.
SMRiP70453. Positions 113-430.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 425265Catalytic By similarity
Add
BLAST

Domaini

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG300643.
GeneTreeiENSGT00730000110321.
HOGENOMiHOG000220881.
HOVERGENiHBG053543.
KOiK01120.
OMAiLCDRQTE.
OrthoDBiEOG7M98G3.
TreeFamiTF314638.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P70453-1) [UniParc]FASTAAdd to Basket

Also known as: PDE7A2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGITLIWCLA LVLIKWITSK RRGAISYDSS DQTALYIRML GDVRVRSRAG    50
FETERRGSHP YIDFRIFHSQ SDIEASVSAR NIRRLLSFQR YLRSSRVFRG 100
ATVCSSLDIL DEDYNGQAKC MLEKVGNWNF DIFLFDRLTN GNSLVSLTFH 150
LFSLHGLIEY FHLDMVKLRR FLVMIQEDYH SQNPYHNAVH AADVTQAMHC 200
YLKEPKLASS VTPWDILLSL IAAATHDLDH PGVNQPFLIK TNHYLATLYK 250
NSSVLENHHW RSAVGLLRES GLFSHLPLES RQEMEAQIGA LILATDISRQ 300
NEYLSLFRSH LDKGDLHLDD GRHRHLVLQM ALKCADICNP CRNWELSKQW 350
SEKVTEEFFH QGDIEKKYHL GVSPLCDRQT ESIANIQIGF MTYLVEPLFT 400
EWARFSDTRL SQTMLGHVGL NKASWKGLQR QQPSSEDANA AFELNSQLLT 450
QENRLS 456
Length:456
Mass (Da):52,486
Last modified:July 27, 2011 - v2
Checksum:i0B84B62094BD9F6E
GO
Isoform 2 (identifier: P70453-2) [UniParc]FASTAAdd to Basket

Also known as: PDE7A1

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MGITLIWCLALVLIKWITSK → MEVCYQLPVLPLDRPVPQHVLSRRGAISFSSSSALFGCPHPRQLSQ

Show »
Length:482
Mass (Da):55,289
Checksum:iE8470DD85BFF7714
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2020MGITL…WITSK → MEVCYQLPVLPLDRPVPQHV LSRRGAISFSSSSALFGCPH PRQLSQ in isoform 2.
VSP_004594Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti407 – 4071D → A in AAB08479. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U68171 mRNA. Translation: AAB08479.1.
AY007702 mRNA. Translation: AAG16295.1.
AK168957 mRNA. Translation: BAE40763.1.
CCDSiCCDS38398.1. [P70453-1]
CCDS50871.1. [P70453-2]
RefSeqiNP_001116231.1. NM_001122759.2. [P70453-2]
NP_032828.2. NM_008802.3. [P70453-1]
UniGeneiMm.355614.

Genome annotation databases

EnsembliENSMUST00000091314; ENSMUSP00000088863; ENSMUSG00000069094. [P70453-1]
ENSMUST00000099195; ENSMUSP00000096800; ENSMUSG00000069094. [P70453-2]
GeneIDi18583.
KEGGimmu:18583.
UCSCiuc008ort.2. mouse. [P70453-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U68171 mRNA. Translation: AAB08479.1 .
AY007702 mRNA. Translation: AAG16295.1 .
AK168957 mRNA. Translation: BAE40763.1 .
CCDSi CCDS38398.1. [P70453-1 ]
CCDS50871.1. [P70453-2 ]
RefSeqi NP_001116231.1. NM_001122759.2. [P70453-2 ]
NP_032828.2. NM_008802.3. [P70453-1 ]
UniGenei Mm.355614.

3D structure databases

ProteinModelPortali P70453.
SMRi P70453. Positions 113-430.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202081. 1 interaction.
IntActi P70453. 1 interaction.

Chemistry

ChEMBLi CHEMBL2040702.

PTM databases

PhosphoSitei P70453.

Proteomic databases

PRIDEi P70453.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000091314 ; ENSMUSP00000088863 ; ENSMUSG00000069094 . [P70453-1 ]
ENSMUST00000099195 ; ENSMUSP00000096800 ; ENSMUSG00000069094 . [P70453-2 ]
GeneIDi 18583.
KEGGi mmu:18583.
UCSCi uc008ort.2. mouse. [P70453-1 ]

Organism-specific databases

CTDi 5150.
MGIi MGI:1202402. Pde7a.

Phylogenomic databases

eggNOGi NOG300643.
GeneTreei ENSGT00730000110321.
HOGENOMi HOG000220881.
HOVERGENi HBG053543.
KOi K01120.
OMAi LCDRQTE.
OrthoDBi EOG7M98G3.
TreeFami TF314638.

Enzyme and pathway databases

UniPathwayi UPA00762 ; UER00747 .

Miscellaneous databases

NextBioi 294452.
PROi P70453.
SOURCEi Search...

Gene expression databases

ArrayExpressi P70453.
Bgeei P70453.
CleanExi MM_PDE7A.
Genevestigatori P70453.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
InterProi IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants."
    Bloom T.J., Beavo J.A.
    Proc. Natl. Acad. Sci. U.S.A. 93:14188-14192(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Skeletal muscle.
  2. "Cloning, characterization, and tissue distribution of mouse phosphodiesterase 7A1."
    Wang P., Wu P., Egan R.W., Billah M.M.
    Biochem. Biophys. Res. Commun. 276:1271-1277(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Brain and Testis.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Amnion.

Entry informationi

Entry nameiPDE7A_MOUSE
AccessioniPrimary (citable) accession number: P70453
Secondary accession number(s): Q3TFY5, Q9ERB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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