Reviewed, UniProtKB/Swiss-Prot P70453 (PDE7A_MOUSE)

Names and origin

Protein names

Recommended name:
    High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A
    EC=3.1.4.17
Alternative name(s):
    P2A

Gene names

Name:

Pde7a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase is highly specific for cAMP and may have a role in muscle signal transduction.
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
Cofactor	Divalent cations.
Enzyme regulation	Insensitive to all selective PDE inhibitors.
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Subunit structure	Interacts with CBFA2T3 By similarity.
Tissue specificity	Widely expressed with highest levels in the skeletal muscle.
Domain	Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	cAMP
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P70453-1)

Also known as: PDE7A2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P70453-2)

Also known as: PDE7A1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MGITLIWCLALVLIKWITSK → MEVCYQLPVLPLDRPVPQHVLSRRGAISFSSSSALFGCPHPRQLSQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A		PRO_0000198834
Regions
	Region	161 – 425	265	Catalytic By similarity
Natural variations
	Alternative sequence	1 – 20	20	MGITL…WITSK → MEVCYQLPVLPLDRPVPQHV LSRRGAISFSSSSALFGCPH PRQLSQ in isoform 2.		VSP_004594
Experimental info
	Sequence conflict	407	1	A → D in AAG16295. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PDE7A2) [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 0B826B96490D9F6E	FASTA	456	52,442
	10 20 30 40 50 60 MGITLIWCLA LVLIKWITSK RRGAISYDSS DQTALYIRML GDVRVRSRAG FETERRGSHP 70 80 90 100 110 120 YIDFRIFHSQ SDIEASVSAR NIRRLLSFQR YLRSSRVFRG ATVCSSLDIL DEDYNGQAKC 130 140 150 160 170 180 MLEKVGNWNF DIFLFDRLTN GNSLVSLTFH LFSLHGLIEY FHLDMVKLRR FLVMIQEDYH 190 200 210 220 230 240 SQNPYHNAVH AADVTQAMHC YLKEPKLASS VTPWDILLSL IAAATHDLDH PGVNQPFLIK 250 260 270 280 290 300 TNHYLATLYK NSSVLENHHW RSAVGLLRES GLFSHLPLES RQEMEAQIGA LILATDISRQ 310 320 330 340 350 360 NEYLSLFRSH LDKGDLHLDD GRHRHLVLQM ALKCADICNP CRNWELSKQW SEKVTEEFFH 370 380 390 400 410 420 QGDIEKKYHL GVSPLCDRQT ESIANIQIGF MTYLVEPLFT EWARFSATRL SQTMLGHVGL 430 440 450 NKASWKGLQR QQPSSEDANA AFELNSQLLT QENRLS
	Isoform 2 (PDE7A1). Checksum: E841D06E864F7714	FASTA	482	55,245
	10 20 30 40 50 60 MEVCYQLPVL PLDRPVPQHV LSRRGAISFS SSSALFGCPH PRQLSQRRGA ISYDSSDQTA 70 80 90 100 110 120 LYIRMLGDVR VRSRAGFETE RRGSHPYIDF RIFHSQSDIE ASVSARNIRR LLSFQRYLRS 130 140 150 160 170 180 SRVFRGATVC SSLDILDEDY NGQAKCMLEK VGNWNFDIFL FDRLTNGNSL VSLTFHLFSL 190 200 210 220 230 240 HGLIEYFHLD MVKLRRFLVM IQEDYHSQNP YHNAVHAADV TQAMHCYLKE PKLASSVTPW 250 260 270 280 290 300 DILLSLIAAA THDLDHPGVN QPFLIKTNHY LATLYKNSSV LENHHWRSAV GLLRESGLFS 310 320 330 340 350 360 HLPLESRQEM EAQIGALILA TDISRQNEYL SLFRSHLDKG DLHLDDGRHR HLVLQMALKC 370 380 390 400 410 420 ADICNPCRNW ELSKQWSEKV TEEFFHQGDI EKKYHLGVSP LCDRQTESIA NIQIGFMTYL 430 440 450 460 470 480 VEPLFTEWAR FSATRLSQTM LGHVGLNKAS WKGLQRQQPS SEDANAAFEL NSQLLTQENR LS

References

[1]	"Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants." Bloom T.J., Beavo J.A. Proc. Natl. Acad. Sci. U.S.A. 93:14188-14192(1996) [PubMed: 8943082] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Skeletal muscle.
[2]	"Cloning, characterization, and tissue distribution of mouse phosphodiesterase 7A1." Wang P., Wu P., Egan R.W., Billah M.M. Biochem. Biophys. Res. Commun. 276:1271-1277(2000) [PubMed: 11027622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain and Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U68171 mRNA. Translation: AAB08479.1. AY007702 mRNA. Translation: AAG16295.1.
IPI	IPI00230552. IPI00762046.
RefSeq	NP_001116231.1.
UniGene	Mm.355614
3D structure databases
HSSP	HSSP built from PDB template 1OYN based on UniProtKB Q08499.
SMR	P70453. Positions 113-429.
ModBase	Search...
Protein-protein interaction databases
STRING	P70453.
PTM databases
PhosphoSite	P70453.
Proteomic databases
PRIDE	P70453.
Genome annotation databases
Ensembl	ENSMUST00000091314; ENSMUSP00000088863; ENSMUSG00000069094; Mus musculus. [Genome view] ENSMUST00000099195; ENSMUSP00000096800; ENSMUSG00000069094; Mus musculus. [Genome view]
GeneID	18583.
KEGG	mmu:18583.
UCSC	uc008ort.1. mouse.
Organism-specific databases
MGI	MGI:1202402. Pde7a.
Phylogenomic databases
HOVERGEN	P70453.
Enzyme and pathway databases
BRENDA	3.1.4.17. 244.
Gene expression databases
ArrayExpress	P70453.
Bgee	P70453.
CleanEx	MM_PDE7A.
Genevestigator	P70453.
GermOnline	ENSMUSG00000069094. Mus musculus.
Family and domain databases
InterPro	IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

PDE7A_MOUSE

Accession

Primary (citable) accession number: P70453
Secondary accession number(s): Q9ERB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	October 13, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)