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Protein

Syntaxin-4

Gene

Stx4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-114516. Disinhibition of SNARE formation.
R-MMU-1236974. ER-Phagosome pathway.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-421837. Clathrin derived vesicle budding.
R-MMU-449147. Signaling by Interleukins.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-4
Gene namesi
Name:Stx4
Synonyms:Stx4a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:893577. Stx4a.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 274CytoplasmicSequence analysisAdd BLAST274
Transmembranei275 – 295Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini296 – 298ExtracellularSequence analysis3

GO - Cellular componenti

  • basolateral plasma membrane Source: MGI
  • cell surface Source: BHF-UCL
  • cytoplasm Source: MGI
  • dendritic spine Source: MGI
  • endosome Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • integral component of membrane Source: GO_Central
  • intracellular Source: MGI
  • lamellipodium Source: MGI
  • lateral loop Source: BHF-UCL
  • membrane Source: MGI
  • myelin sheath adaxonal region Source: BHF-UCL
  • perinuclear region of cytoplasm Source: UniProtKB
  • plasma membrane Source: MGI
  • SNARE complex Source: UniProtKB
  • somatodendritic compartment Source: MGI
  • specific granule Source: MGI
  • storage vacuole Source: MGI
  • synapse Source: MGI
  • synaptic vesicle Source: GO_Central
  • trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002102031 – 298Syntaxin-4Add BLAST298

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1
Modified residuei29PhosphoserineCombined sources1
Modified residuei36PhosphoserineBy similarity1
Modified residuei117PhosphoserineCombined sources1
Modified residuei208PhosphoserineCombined sources1
Modified residuei248PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP70452.
MaxQBiP70452.
PaxDbiP70452.
PeptideAtlasiP70452.
PRIDEiP70452.

PTM databases

iPTMnetiP70452.
PhosphoSitePlusiP70452.
SwissPalmiP70452.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030805.
CleanExiMM_STX4A.
ExpressionAtlasiP70452. baseline and differential.
GenevisibleiP70452. MM.

Interactioni

Subunit structurei

Interacts with STXBP6. Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis (By similarity). Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with VAMP2. Interacts with SNAP23 and SNAPIN. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2.By similarity8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CenpfQ155P79EBI-645716,EBI-2211248
Snap23O090442EBI-645716,EBI-1812522

GO - Molecular functioni

  • myosin binding Source: GO_Central
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: UniProtKB

Protein-protein interaction databases

BioGridi203563. 5 interactors.
DIPiDIP-41399N.
IntActiP70452. 8 interactors.
MINTiMINT-269272.
STRINGi10090.ENSMUSP00000033075.

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 8Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PUJX-ray3.31C/D1-10[»]
3PUKX-ray3.05C/D1-10[»]
ProteinModelPortaliP70452.
SMRiP70452.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70452.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini200 – 262t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni154 – 298Interaction with CENPF1 PublicationAdd BLAST145

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili38 – 163Sequence analysisAdd BLAST126

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0810. Eukaryota.
COG5074. LUCA.
GeneTreeiENSGT00760000119200.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiP70452.
KOiK13502.
OMAiQKVQTIR.
OrthoDBiEOG091G0EUK.
PhylomeDBiP70452.
TreeFamiTF313763.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70452-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDRTHELRQ GDNISDDEDE VRVALVVHSG AARLGSPDDE FFQKVQTIRQ
60 70 80 90 100
TMAKLESKVR ELEKQQVTIL ATPLPEESMK QGLQNLREEI KQLGREVRAQ
110 120 130 140 150
LKAIEPQKEE ADENYNSVNT RMKKTQHGVL SQQFVELINK CNSMQSEYRE
160 170 180 190 200
KNVERIRRQL KITNAGMVSD EELEQMLDSG QSEVFVSNIL KDTQVTRQAL
210 220 230 240 250
NEISARHSEI QQLERSIREL HEIFTFLATE VEMQGEMINR IEKNILSSAD
260 270 280 290
YVERGQEHVK IALENQKKAR KKKVMIAICV SVTVLILAVI IGITITVG
Length:298
Mass (Da):34,165
Last modified:February 1, 1997 - v1
Checksum:iFCD1477E1126CEC1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47T → I in AAH52023 (PubMed:15489334).Curated1
Sequence conflicti141C → Y in BAE36660 (PubMed:16141072).Curated1
Sequence conflicti281S → Y in BAE26743 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76832 mRNA. Translation: AAB18991.1.
AK145909 mRNA. Translation: BAE26743.1.
AK161971 mRNA. Translation: BAE36660.1.
BC005791 mRNA. Translation: AAH05791.1.
BC011491 mRNA. Translation: AAH11491.1.
BC052023 mRNA. Translation: AAH52023.1.
CCDSiCCDS21880.1.
RefSeqiNP_033320.1. NM_009294.3.
UniGeneiMm.24867.

Genome annotation databases

EnsembliENSMUST00000033075; ENSMUSP00000033075; ENSMUSG00000030805.
ENSMUST00000121705; ENSMUSP00000112927; ENSMUSG00000030805.
GeneIDi20909.
KEGGimmu:20909.
UCSCiuc009jwy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76832 mRNA. Translation: AAB18991.1.
AK145909 mRNA. Translation: BAE26743.1.
AK161971 mRNA. Translation: BAE36660.1.
BC005791 mRNA. Translation: AAH05791.1.
BC011491 mRNA. Translation: AAH11491.1.
BC052023 mRNA. Translation: AAH52023.1.
CCDSiCCDS21880.1.
RefSeqiNP_033320.1. NM_009294.3.
UniGeneiMm.24867.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PUJX-ray3.31C/D1-10[»]
3PUKX-ray3.05C/D1-10[»]
ProteinModelPortaliP70452.
SMRiP70452.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203563. 5 interactors.
DIPiDIP-41399N.
IntActiP70452. 8 interactors.
MINTiMINT-269272.
STRINGi10090.ENSMUSP00000033075.

PTM databases

iPTMnetiP70452.
PhosphoSitePlusiP70452.
SwissPalmiP70452.

Proteomic databases

EPDiP70452.
MaxQBiP70452.
PaxDbiP70452.
PeptideAtlasiP70452.
PRIDEiP70452.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033075; ENSMUSP00000033075; ENSMUSG00000030805.
ENSMUST00000121705; ENSMUSP00000112927; ENSMUSG00000030805.
GeneIDi20909.
KEGGimmu:20909.
UCSCiuc009jwy.1. mouse.

Organism-specific databases

CTDi20909.
MGIiMGI:893577. Stx4a.

Phylogenomic databases

eggNOGiKOG0810. Eukaryota.
COG5074. LUCA.
GeneTreeiENSGT00760000119200.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiP70452.
KOiK13502.
OMAiQKVQTIR.
OrthoDBiEOG091G0EUK.
PhylomeDBiP70452.
TreeFamiTF313763.

Enzyme and pathway databases

ReactomeiR-MMU-114516. Disinhibition of SNARE formation.
R-MMU-1236974. ER-Phagosome pathway.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-421837. Clathrin derived vesicle budding.
R-MMU-449147. Signaling by Interleukins.

Miscellaneous databases

EvolutionaryTraceiP70452.
PROiP70452.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030805.
CleanExiMM_STX4A.
ExpressionAtlasiP70452. baseline and differential.
GenevisibleiP70452. MM.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTX4_MOUSE
AccessioniPrimary (citable) accession number: P70452
Secondary accession number(s): Q3TSL5, Q3UKQ8, Q80WT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.