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P70452

- STX4_MOUSE

UniProt

P70452 - STX4_MOUSE

Protein

Syntaxin-4

Gene

Stx4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones.3 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. SNARE binding Source: UniProtKB

    GO - Biological processi

    1. intracellular protein transport Source: InterPro
    2. SNARE complex assembly Source: BHF-UCL
    3. synaptic vesicle exocytosis Source: MGI

    Keywords - Biological processi

    Neurotransmitter transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_215302. Disinhibition of SNARE formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Syntaxin-4
    Gene namesi
    Name:Stx4
    Synonyms:Stx4a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:893577. Stx4a.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: MGI
    2. cell surface Source: BHF-UCL
    3. cytoplasm Source: MGI
    4. integral component of membrane Source: UniProtKB-KW
    5. lateral loop Source: BHF-UCL
    6. myelin sheath adaxonal region Source: BHF-UCL
    7. perinuclear region of cytoplasm Source: UniProtKB
    8. plasma membrane Source: MGI
    9. SNARE complex Source: UniProtKB
    10. specific granule Source: Ensembl
    11. trans-Golgi network Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 298298Syntaxin-4PRO_0000210203Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei15 – 151Phosphoserine2 Publications
    Modified residuei29 – 291Phosphoserine1 Publication
    Modified residuei117 – 1171Phosphoserine1 Publication
    Modified residuei248 – 2481Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP70452.
    PaxDbiP70452.
    PRIDEiP70452.

    PTM databases

    PhosphoSiteiP70452.

    Expressioni

    Gene expression databases

    ArrayExpressiP70452.
    BgeeiP70452.
    CleanExiMM_STX4A.
    GenevestigatoriP70452.

    Interactioni

    Subunit structurei

    Interacts with STXBP6. Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis By similarity. Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with VAMP2. Interacts with SNAP23 and SNAPIN. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2.By similarity8 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CenpfQ155P79EBI-645716,EBI-2211248

    Protein-protein interaction databases

    BioGridi203563. 5 interactions.
    DIPiDIP-41399N.
    IntActiP70452. 6 interactions.
    MINTiMINT-269272.

    Structurei

    Secondary structure

    1
    298
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 84

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3PUJX-ray3.31C/D1-10[»]
    3PUKX-ray3.05C/D1-10[»]
    ProteinModelPortaliP70452.
    SMRiP70452. Positions 39-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP70452.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 274274CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini296 – 2983ExtracellularSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei275 – 29521Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini200 – 26263t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni154 – 298145Interaction with CENPFAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili38 – 163126Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the syntaxin family.Curated
    Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5074.
    GeneTreeiENSGT00730000110677.
    HOGENOMiHOG000286023.
    HOVERGENiHBG000497.
    InParanoidiQ3TSL5.
    KOiK13502.
    OMAiINIRMQR.
    OrthoDBiEOG7X9G7R.
    PhylomeDBiP70452.
    TreeFamiTF313763.

    Family and domain databases

    InterProiIPR006012. Syntaxin/epimorphin_CS.
    IPR006011. Syntaxin_N.
    IPR010989. t-SNARE.
    IPR000727. T_SNARE_dom.
    [Graphical view]
    PfamiPF05739. SNARE. 1 hit.
    PF00804. Syntaxin. 1 hit.
    [Graphical view]
    SMARTiSM00503. SynN. 1 hit.
    SM00397. t_SNARE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47661. SSF47661. 1 hit.
    PROSITEiPS00914. SYNTAXIN. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P70452-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRDRTHELRQ GDNISDDEDE VRVALVVHSG AARLGSPDDE FFQKVQTIRQ    50
    TMAKLESKVR ELEKQQVTIL ATPLPEESMK QGLQNLREEI KQLGREVRAQ 100
    LKAIEPQKEE ADENYNSVNT RMKKTQHGVL SQQFVELINK CNSMQSEYRE 150
    KNVERIRRQL KITNAGMVSD EELEQMLDSG QSEVFVSNIL KDTQVTRQAL 200
    NEISARHSEI QQLERSIREL HEIFTFLATE VEMQGEMINR IEKNILSSAD 250
    YVERGQEHVK IALENQKKAR KKKVMIAICV SVTVLILAVI IGITITVG 298
    Length:298
    Mass (Da):34,165
    Last modified:February 1, 1997 - v1
    Checksum:iFCD1477E1126CEC1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti47 – 471T → I in AAH52023. (PubMed:15489334)Curated
    Sequence conflicti141 – 1411C → Y in BAE36660. (PubMed:16141072)Curated
    Sequence conflicti281 – 2811S → Y in BAE26743. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U76832 mRNA. Translation: AAB18991.1.
    AK145909 mRNA. Translation: BAE26743.1.
    AK161971 mRNA. Translation: BAE36660.1.
    BC005791 mRNA. Translation: AAH05791.1.
    BC011491 mRNA. Translation: AAH11491.1.
    BC052023 mRNA. Translation: AAH52023.1.
    CCDSiCCDS21880.1.
    RefSeqiNP_033320.1. NM_009294.3.
    UniGeneiMm.24867.

    Genome annotation databases

    EnsembliENSMUST00000033075; ENSMUSP00000033075; ENSMUSG00000030805.
    ENSMUST00000121705; ENSMUSP00000112927; ENSMUSG00000030805.
    GeneIDi20909.
    KEGGimmu:20909.
    UCSCiuc009jwy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U76832 mRNA. Translation: AAB18991.1 .
    AK145909 mRNA. Translation: BAE26743.1 .
    AK161971 mRNA. Translation: BAE36660.1 .
    BC005791 mRNA. Translation: AAH05791.1 .
    BC011491 mRNA. Translation: AAH11491.1 .
    BC052023 mRNA. Translation: AAH52023.1 .
    CCDSi CCDS21880.1.
    RefSeqi NP_033320.1. NM_009294.3.
    UniGenei Mm.24867.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3PUJ X-ray 3.31 C/D 1-10 [» ]
    3PUK X-ray 3.05 C/D 1-10 [» ]
    ProteinModelPortali P70452.
    SMRi P70452. Positions 39-292.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203563. 5 interactions.
    DIPi DIP-41399N.
    IntActi P70452. 6 interactions.
    MINTi MINT-269272.

    PTM databases

    PhosphoSitei P70452.

    Proteomic databases

    MaxQBi P70452.
    PaxDbi P70452.
    PRIDEi P70452.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033075 ; ENSMUSP00000033075 ; ENSMUSG00000030805 .
    ENSMUST00000121705 ; ENSMUSP00000112927 ; ENSMUSG00000030805 .
    GeneIDi 20909.
    KEGGi mmu:20909.
    UCSCi uc009jwy.1. mouse.

    Organism-specific databases

    CTDi 20909.
    MGIi MGI:893577. Stx4a.

    Phylogenomic databases

    eggNOGi COG5074.
    GeneTreei ENSGT00730000110677.
    HOGENOMi HOG000286023.
    HOVERGENi HBG000497.
    InParanoidi Q3TSL5.
    KOi K13502.
    OMAi INIRMQR.
    OrthoDBi EOG7X9G7R.
    PhylomeDBi P70452.
    TreeFami TF313763.

    Enzyme and pathway databases

    Reactomei REACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_215302. Disinhibition of SNARE formation.

    Miscellaneous databases

    EvolutionaryTracei P70452.
    NextBioi 299793.
    PROi P70452.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70452.
    Bgeei P70452.
    CleanExi MM_STX4A.
    Genevestigatori P70452.

    Family and domain databases

    InterProi IPR006012. Syntaxin/epimorphin_CS.
    IPR006011. Syntaxin_N.
    IPR010989. t-SNARE.
    IPR000727. T_SNARE_dom.
    [Graphical view ]
    Pfami PF05739. SNARE. 1 hit.
    PF00804. Syntaxin. 1 hit.
    [Graphical view ]
    SMARTi SM00503. SynN. 1 hit.
    SM00397. t_SNARE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47661. SSF47661. 1 hit.
    PROSITEi PS00914. SYNTAXIN. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of Munc-18c and syntaxin-4 in 3T3-L1 adipocytes. Putative role in insulin-dependent movement of GLUT-4."
      Tellam J.T., Macaulay S.L., McIntosh S., Hewish D.R., Ward C.W., James D.E.
      J. Biol. Chem. 272:6179-6186(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH STXB3, SUBCELLULAR LOCATION.
      Tissue: Adipocyte.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Olfactory bulb and Placenta.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6, Czech II and FVB/N.
      Tissue: Brain and Mammary gland.
    4. "Synip: a novel insulin-regulated syntaxin 4-binding protein mediating GLUT4 translocation in adipocytes."
      Min J., Okada S., Kanzaki M., Elmendorf J.S., Coker K.J., Ceresa B.P., Syu L.-J., Noda Y., Saltiel A.R., Pessin J.E.
      Mol. Cell 3:751-760(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH STXBP4; SNAP23 AND VAMP2.
    5. "Identification and characterization of Snapin as a ubiquitously expressed SNARE-binding protein that interacts with SNAP23 in non-neuronal cells."
      Buxton P., Zhang X.-M., Walsh B., Sriratana A., Schenberg I., Manickam E., Rowe T.
      Biochem. J. 375:433-440(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNAP23 AND SNAPIN.
    6. "A role of VAMP8/endobrevin in regulated exocytosis of pancreatic acinar cells."
      Wang C.-C., Ng C.P., Lu L., Atlashkin V., Zhang W., Seet L.-F., Hong W.
      Dev. Cell 7:359-371(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH VAMP8 AND SNAP23.
    7. "Mammalian homolog of Drosophila tumor suppressor lethal (2) giant larvae interacts with basolateral exocytic machinery in Madin-Darby canine kidney cells."
      Musch A., Cohen D., Yeaman C., Nelson W.J., Rodriguez-Boulan E., Brennwald P.J.
      Mol. Biol. Cell 13:158-168(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LLGL1.
    8. "Doc2beta is a novel Munc18c-interacting partner and positive effector of syntaxin 4-mediated exocytosis."
      Ke B., Oh E., Thurmond D.C.
      J. Biol. Chem. 282:21786-21797(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH STXBP3 AND VAMP2.
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    10. "Murine CENPF interacts with syntaxin 4 in the regulation of vesicular transport."
      Pooley R.D., Moynihan K.L., Soukoulis V., Reddy S., Francis R., Lo C., Ma L.-J., Bader D.M.
      J. Cell Sci. 121:3413-3421(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CENPF, SUBCELLULAR LOCATION.
    11. "DOC2B: a novel syntaxin-4 binding protein mediating insulin-regulated GLUT4 vesicle fusion in adipocytes."
      Fukuda N., Emoto M., Nakamori Y., Taguchi A., Miyamoto S., Uraki S., Oka Y., Tanizawa Y.
      Diabetes 58:377-384(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DOC2B.
    12. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; SER-29 AND SER-248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiSTX4_MOUSE
    AccessioniPrimary (citable) accession number: P70452
    Secondary accession number(s): Q3TSL5, Q3UKQ8, Q80WT8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3