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Protein

Syntaxin-4

Gene

Stx4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones.3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processNeurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-114516 Disinhibition of SNARE formation
R-MMU-1236974 ER-Phagosome pathway
R-MMU-421837 Clathrin derived vesicle budding
R-MMU-449836 Other interleukin signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-4
Gene namesi
Name:Stx4
Synonyms:Stx4a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:893577 Stx4a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 274CytoplasmicSequence analysisAdd BLAST274
Transmembranei275 – 295Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini296 – 298ExtracellularSequence analysis3

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002102031 – 298Syntaxin-4Add BLAST298

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1
Modified residuei29PhosphoserineCombined sources1
Modified residuei36PhosphoserineBy similarity1
Modified residuei117PhosphoserineCombined sources1
Modified residuei208PhosphoserineCombined sources1
Modified residuei248PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP70452
MaxQBiP70452
PaxDbiP70452
PeptideAtlasiP70452
PRIDEiP70452

PTM databases

iPTMnetiP70452
PhosphoSitePlusiP70452
SwissPalmiP70452

Expressioni

Gene expression databases

BgeeiENSMUSG00000030805
CleanExiMM_STX4A
ExpressionAtlasiP70452 baseline and differential
GenevisibleiP70452 MM

Interactioni

Subunit structurei

Interacts with STXBP6. Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis (By similarity). Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with VAMP2. Interacts with SNAP23 and SNAPIN. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2.By similarity8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: UniProtKB

Protein-protein interaction databases

BioGridi2035635 interactors.
CORUMiP70452
DIPiDIP-41399N
IntActiP70452 9 interactors.
MINTiP70452
STRINGi10090.ENSMUSP00000033075

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 8Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PUJX-ray3.31C/D1-10[»]
3PUKX-ray3.05C/D1-10[»]
ProteinModelPortaliP70452
SMRiP70452
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70452

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini200 – 262t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni154 – 298Interaction with CENPF1 PublicationAdd BLAST145

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili38 – 163Sequence analysisAdd BLAST126

Sequence similaritiesi

Belongs to the syntaxin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0810 Eukaryota
COG5074 LUCA
GeneTreeiENSGT00760000119200
HOGENOMiHOG000286023
HOVERGENiHBG000497
InParanoidiP70452
KOiK13502
OMAiQYLAMEV
OrthoDBiEOG091G0EUK
PhylomeDBiP70452
TreeFamiTF313763

Family and domain databases

InterProiView protein in InterPro
IPR010989 SNARE
IPR006012 Syntaxin/epimorphin_CS
IPR006011 Syntaxin_N
IPR000727 T_SNARE_dom
PfamiView protein in Pfam
PF05739 SNARE, 1 hit
PF00804 Syntaxin, 1 hit
SMARTiView protein in SMART
SM00503 SynN, 1 hit
SM00397 t_SNARE, 1 hit
SUPFAMiSSF47661 SSF47661, 1 hit
PROSITEiView protein in PROSITE
PS00914 SYNTAXIN, 1 hit
PS50192 T_SNARE, 1 hit

Sequencei

Sequence statusi: Complete.

P70452-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDRTHELRQ GDNISDDEDE VRVALVVHSG AARLGSPDDE FFQKVQTIRQ
60 70 80 90 100
TMAKLESKVR ELEKQQVTIL ATPLPEESMK QGLQNLREEI KQLGREVRAQ
110 120 130 140 150
LKAIEPQKEE ADENYNSVNT RMKKTQHGVL SQQFVELINK CNSMQSEYRE
160 170 180 190 200
KNVERIRRQL KITNAGMVSD EELEQMLDSG QSEVFVSNIL KDTQVTRQAL
210 220 230 240 250
NEISARHSEI QQLERSIREL HEIFTFLATE VEMQGEMINR IEKNILSSAD
260 270 280 290
YVERGQEHVK IALENQKKAR KKKVMIAICV SVTVLILAVI IGITITVG
Length:298
Mass (Da):34,165
Last modified:February 1, 1997 - v1
Checksum:iFCD1477E1126CEC1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47T → I in AAH52023 (PubMed:15489334).Curated1
Sequence conflicti141C → Y in BAE36660 (PubMed:16141072).Curated1
Sequence conflicti281S → Y in BAE26743 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76832 mRNA Translation: AAB18991.1
AK145909 mRNA Translation: BAE26743.1
AK161971 mRNA Translation: BAE36660.1
BC005791 mRNA Translation: AAH05791.1
BC011491 mRNA Translation: AAH11491.1
BC052023 mRNA Translation: AAH52023.1
CCDSiCCDS21880.1
RefSeqiNP_033320.1, NM_009294.3
UniGeneiMm.24867

Genome annotation databases

EnsembliENSMUST00000033075; ENSMUSP00000033075; ENSMUSG00000030805
ENSMUST00000121705; ENSMUSP00000112927; ENSMUSG00000030805
GeneIDi20909
KEGGimmu:20909
UCSCiuc009jwy.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSTX4_MOUSE
AccessioniPrimary (citable) accession number: P70452
Secondary accession number(s): Q3TSL5, Q3UKQ8, Q80WT8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome