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Protein

Syntaxin-4

Gene

Stx4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones.3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processNeurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-114516. Disinhibition of SNARE formation.
R-MMU-1236974. ER-Phagosome pathway.
R-MMU-421837. Clathrin derived vesicle budding.
R-MMU-449836. Other interleukin signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-4
Gene namesi
Name:Stx4
Synonyms:Stx4a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:893577. Stx4a.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 274CytoplasmicSequence analysisAdd BLAST274
Transmembranei275 – 295Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini296 – 298ExtracellularSequence analysis3

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002102031 – 298Syntaxin-4Add BLAST298

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1
Modified residuei29PhosphoserineCombined sources1
Modified residuei36PhosphoserineBy similarity1
Modified residuei117PhosphoserineCombined sources1
Modified residuei208PhosphoserineCombined sources1
Modified residuei248PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP70452.
MaxQBiP70452.
PaxDbiP70452.
PeptideAtlasiP70452.
PRIDEiP70452.

PTM databases

iPTMnetiP70452.
PhosphoSitePlusiP70452.
SwissPalmiP70452.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030805.
CleanExiMM_STX4A.
ExpressionAtlasiP70452. baseline and differential.
GenevisibleiP70452. MM.

Interactioni

Subunit structurei

Interacts with STXBP6. Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis (By similarity). Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with VAMP2. Interacts with SNAP23 and SNAPIN. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2.By similarity8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: UniProtKB

Protein-protein interaction databases

BioGridi203563. 5 interactors.
CORUMiP70452.
DIPiDIP-41399N.
IntActiP70452. 9 interactors.
MINTiMINT-269272.
STRINGi10090.ENSMUSP00000033075.

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 8Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PUJX-ray3.31C/D1-10[»]
3PUKX-ray3.05C/D1-10[»]
ProteinModelPortaliP70452.
SMRiP70452.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70452.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini200 – 262t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni154 – 298Interaction with CENPF1 PublicationAdd BLAST145

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili38 – 163Sequence analysisAdd BLAST126

Sequence similaritiesi

Belongs to the syntaxin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0810. Eukaryota.
COG5074. LUCA.
GeneTreeiENSGT00760000119200.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiP70452.
KOiK13502.
OMAiQYLAMEV.
OrthoDBiEOG091G0EUK.
PhylomeDBiP70452.
TreeFamiTF313763.

Family and domain databases

InterProiView protein in InterPro
IPR010989. SNARE.
IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR000727. T_SNARE_dom.
PfamiView protein in Pfam
PF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
SMARTiView protein in SMART
SM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiView protein in PROSITE
PS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.

Sequencei

Sequence statusi: Complete.

P70452-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDRTHELRQ GDNISDDEDE VRVALVVHSG AARLGSPDDE FFQKVQTIRQ
60 70 80 90 100
TMAKLESKVR ELEKQQVTIL ATPLPEESMK QGLQNLREEI KQLGREVRAQ
110 120 130 140 150
LKAIEPQKEE ADENYNSVNT RMKKTQHGVL SQQFVELINK CNSMQSEYRE
160 170 180 190 200
KNVERIRRQL KITNAGMVSD EELEQMLDSG QSEVFVSNIL KDTQVTRQAL
210 220 230 240 250
NEISARHSEI QQLERSIREL HEIFTFLATE VEMQGEMINR IEKNILSSAD
260 270 280 290
YVERGQEHVK IALENQKKAR KKKVMIAICV SVTVLILAVI IGITITVG
Length:298
Mass (Da):34,165
Last modified:February 1, 1997 - v1
Checksum:iFCD1477E1126CEC1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47T → I in AAH52023 (PubMed:15489334).Curated1
Sequence conflicti141C → Y in BAE36660 (PubMed:16141072).Curated1
Sequence conflicti281S → Y in BAE26743 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76832 mRNA. Translation: AAB18991.1.
AK145909 mRNA. Translation: BAE26743.1.
AK161971 mRNA. Translation: BAE36660.1.
BC005791 mRNA. Translation: AAH05791.1.
BC011491 mRNA. Translation: AAH11491.1.
BC052023 mRNA. Translation: AAH52023.1.
CCDSiCCDS21880.1.
RefSeqiNP_033320.1. NM_009294.3.
UniGeneiMm.24867.

Genome annotation databases

EnsembliENSMUST00000033075; ENSMUSP00000033075; ENSMUSG00000030805.
ENSMUST00000121705; ENSMUSP00000112927; ENSMUSG00000030805.
GeneIDi20909.
KEGGimmu:20909.
UCSCiuc009jwy.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiSTX4_MOUSE
AccessioniPrimary (citable) accession number: P70452
Secondary accession number(s): Q3TSL5, Q3UKQ8, Q80WT8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 22, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families