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Protein

BH3-interacting domain death agonist

Gene

Bid

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c.1 Publication

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • apoptotic mitochondrial changes Source: MGI
  • brain development Source: Ensembl
  • establishment of protein localization to membrane Source: MGI
  • extrinsic apoptotic signaling pathway Source: MGI
  • glial cell apoptotic process Source: Ensembl
  • hepatocyte apoptotic process Source: MGI
  • mitochondrial ATP synthesis coupled electron transport Source: MGI
  • mitochondrial outer membrane permeabilization Source: MGI
  • negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of apoptotic signaling pathway Source: MGI
  • positive regulation of extrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of fibroblast apoptotic process Source: MGI
  • positive regulation of mitochondrial membrane potential Source: MGI
  • positive regulation of protein homooligomerization Source: BHF-UCL
  • positive regulation of protein oligomerization Source: MGI
  • positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
  • protein homooligomerization Source: BHF-UCL
  • protein targeting to mitochondrion Source: MGI
  • regulation of apoptotic process Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of G1/S transition of mitotic cell cycle Source: MGI
  • regulation of mitochondrial membrane permeability involved in apoptotic process Source: MGI
  • regulation of protein oligomerization Source: UniProtKB
  • release of cytochrome c from mitochondria Source: CACAO
  • response to estradiol Source: Ensembl
  • signal transduction in response to DNA damage Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiR-MMU-111447. Activation of BAD and translocation to mitochondria.
R-MMU-111452. Activation and oligomerization of BAK protein.
R-MMU-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
R-MMU-114294. Activation, translocation and oligomerization of BAX.
R-MMU-75108. Activation, myristolyation of BID and translocation to mitochondria.

Names & Taxonomyi

Protein namesi
Recommended name:
BH3-interacting domain death agonist
Alternative name(s):
p22 BID
Short name:
BID
Cleaved into the following 3 chains:
Alternative name(s):
p15 BID
Alternative name(s):
p13 BID
Alternative name(s):
p11 BID
Gene namesi
Name:Bid
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:108093. Bid.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • integral component of mitochondrial membrane Source: BHF-UCL
  • membrane Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi98D → A: Loss of proteolytical cleavage leading to the production of p11 BID. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001431021 – 195BH3-interacting domain death agonistAdd BLAST195
ChainiPRO_000022323661 – 195BH3-interacting domain death agonist p15Add BLAST135
ChainiPRO_000022323576 – 195BH3-interacting domain death agonist p13Add BLAST120
ChainiPRO_000022323499 – 195BH3-interacting domain death agonist p11Add BLAST97

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei78PhosphoserineBy similarity1

Post-translational modificationi

TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.1 Publication
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei60 – 61Cleavage2
Sitei75 – 76Cleavage2
Sitei98 – 99Cleavage2

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP70444.
MaxQBiP70444.
PaxDbiP70444.
PeptideAtlasiP70444.
PRIDEiP70444.

PTM databases

iPTMnetiP70444.
PhosphoSitePlusiP70444.

Miscellaneous databases

PMAP-CutDBP70444.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004446.
CleanExiMM_BID.
ExpressionAtlasiP70444. baseline and differential.
GenevisibleiP70444. MM.

Interactioni

Subunit structurei

Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2. p15 BID interacts with ITCH.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BAXQ078122EBI-2128640,EBI-516580From a different organism.
ITCHQ96J022EBI-783400,EBI-1564678From a different organism.

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi198349. 6 interactors.
DIPiDIP-29808N.
IntActiP70444. 5 interactors.
MINTiMINT-143107.
STRINGi10090.ENSMUSP00000004560.

Structurei

Secondary structure

1195
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 27Combined sources13
Beta strandi28 – 30Combined sources3
Helixi32 – 39Combined sources8
Helixi79 – 99Combined sources21
Beta strandi103 – 105Combined sources3
Helixi106 – 113Combined sources8
Beta strandi116 – 122Combined sources7
Helixi125 – 137Combined sources13
Helixi142 – 162Combined sources21
Helixi167 – 180Combined sources14
Helixi185 – 192Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DDBNMR-A1-195[»]
2VOIX-ray2.10B76-109[»]
ProteinModelPortaliP70444.
SMRiP70444.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70444.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi87 – 100BH3Add BLAST14

Domaini

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. Apoptotic members of the Bcl-2 family.

Phylogenomic databases

eggNOGiENOG410IVZ7. Eukaryota.
ENOG410ZF8F. LUCA.
GeneTreeiENSGT00390000002868.
HOGENOMiHOG000010016.
HOVERGENiHBG001703.
InParanoidiP70444.
KOiK04726.
OMAiRDVFRTT.
OrthoDBiEOG091G0LRX.
PhylomeDBiP70444.
TreeFamiTF102047.

Family and domain databases

InterProiIPR020728. Bcl2_BH3_motif_CS.
IPR010479. BID.
[Graphical view]
PfamiPF06393. BID. 1 hit.
[Graphical view]
PIRSFiPIRSF038018. BID. 1 hit.
PROSITEiPS01259. BH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70444-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSEVSNGSG LGAEHITDLL VFGFLQSSGC TRQELEVLGR ELPVQAYWEA
60 70 80 90 100
DLEDELQTDG SQASRSFNQG RIEPDSESQE EIIHNIARHL AQIGDEMDHN
110 120 130 140 150
IQPTLVRQLA AQFMNGSLSE EDKRNCLAKA LDEVKTAFPR DMENDKAMLI
160 170 180 190
MTMLLAKKVA SHAPSLLRDV FHTTVNFINQ NLFSYVRNLV RNEMD
Length:195
Mass (Da):21,952
Last modified:February 21, 2006 - v2
Checksum:i52F412714FB867F3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14E → K in AAC71064 (PubMed:8918887).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75506 mRNA. Translation: AAC71064.1.
AK045731 mRNA. Translation: BAC32475.1.
AK051076 mRNA. Translation: BAC34518.1.
AK052356 mRNA. Translation: BAC34955.1.
AK077657 mRNA. Translation: BAC36932.1.
AK161235 mRNA. Translation: BAE36258.1.
BC002031 mRNA. Translation: AAH02031.1.
CCDSiCCDS20486.1.
RefSeqiNP_031570.2. NM_007544.3.
XP_006505472.1. XM_006505409.2.
XP_006505473.1. XM_006505410.3.
XP_006505474.1. XM_006505411.3.
UniGeneiMm.235081.

Genome annotation databases

EnsembliENSMUST00000004560; ENSMUSP00000004560; ENSMUSG00000004446.
ENSMUST00000160684; ENSMUSP00000125731; ENSMUSG00000004446.
GeneIDi12122.
KEGGimmu:12122.
UCSCiuc009dnv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75506 mRNA. Translation: AAC71064.1.
AK045731 mRNA. Translation: BAC32475.1.
AK051076 mRNA. Translation: BAC34518.1.
AK052356 mRNA. Translation: BAC34955.1.
AK077657 mRNA. Translation: BAC36932.1.
AK161235 mRNA. Translation: BAE36258.1.
BC002031 mRNA. Translation: AAH02031.1.
CCDSiCCDS20486.1.
RefSeqiNP_031570.2. NM_007544.3.
XP_006505472.1. XM_006505409.2.
XP_006505473.1. XM_006505410.3.
XP_006505474.1. XM_006505411.3.
UniGeneiMm.235081.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DDBNMR-A1-195[»]
2VOIX-ray2.10B76-109[»]
ProteinModelPortaliP70444.
SMRiP70444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198349. 6 interactors.
DIPiDIP-29808N.
IntActiP70444. 5 interactors.
MINTiMINT-143107.
STRINGi10090.ENSMUSP00000004560.

PTM databases

iPTMnetiP70444.
PhosphoSitePlusiP70444.

Proteomic databases

EPDiP70444.
MaxQBiP70444.
PaxDbiP70444.
PeptideAtlasiP70444.
PRIDEiP70444.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004560; ENSMUSP00000004560; ENSMUSG00000004446.
ENSMUST00000160684; ENSMUSP00000125731; ENSMUSG00000004446.
GeneIDi12122.
KEGGimmu:12122.
UCSCiuc009dnv.1. mouse.

Organism-specific databases

CTDi637.
MGIiMGI:108093. Bid.

Phylogenomic databases

eggNOGiENOG410IVZ7. Eukaryota.
ENOG410ZF8F. LUCA.
GeneTreeiENSGT00390000002868.
HOGENOMiHOG000010016.
HOVERGENiHBG001703.
InParanoidiP70444.
KOiK04726.
OMAiRDVFRTT.
OrthoDBiEOG091G0LRX.
PhylomeDBiP70444.
TreeFamiTF102047.

Enzyme and pathway databases

ReactomeiR-MMU-111447. Activation of BAD and translocation to mitochondria.
R-MMU-111452. Activation and oligomerization of BAK protein.
R-MMU-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
R-MMU-114294. Activation, translocation and oligomerization of BAX.
R-MMU-75108. Activation, myristolyation of BID and translocation to mitochondria.

Miscellaneous databases

EvolutionaryTraceiP70444.
PMAP-CutDBP70444.
PROiP70444.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004446.
CleanExiMM_BID.
ExpressionAtlasiP70444. baseline and differential.
GenevisibleiP70444. MM.

Family and domain databases

InterProiIPR020728. Bcl2_BH3_motif_CS.
IPR010479. BID.
[Graphical view]
PfamiPF06393. BID. 1 hit.
[Graphical view]
PIRSFiPIRSF038018. BID. 1 hit.
PROSITEiPS01259. BH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBID_MOUSE
AccessioniPrimary (citable) accession number: P70444
Secondary accession number(s): Q99M39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 21, 2006
Last modified: November 30, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.