Reviewed, UniProtKB/Swiss-Prot P70444 (BID_MOUSE)

Names and origin

Protein names

Recommended name:
    BH3-interacting domain death agonist
Alternative name(s):
    p22 BID
      Short name=BID
Cleaved into the following 3 chains:
    1- Recommended name:
            BH3-interacting domain death agonist p15
        Alternative name(s):
            p15 BID
    2- Recommended name:
            BH3-interacting domain death agonist p13
        Alternative name(s):
            p13 BID
    3- Recommended name:
            BH3-interacting domain death agonist p11
        Alternative name(s):
            p11 BID

Gene names

Name:

Bid

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c. Ref.4
Subunit structure	Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2.
Subcellular location	Cytoplasm. Mitochondrion membrane. Note: When uncleaved, it is predominantly cytoplasmic. p15 BID translocates to mitochondria as an integral membrane protein. p13 and p22 BID are associated with the mitochondrial membrane. Ref.4
Domain	Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. Apoptotic members of the Bcl-2 family.
Post-translational modification	TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products. Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Membrane Mitochondrion
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	positive regulation of apoptosis Inferred from electronic annotation. Source: InterPro protein targeting to mitochondrion Inferred from mutant phenotype. Source: MGI regulation of mitochondrial membrane permeability Inferred from genetic interaction. Source: MGI release of cytochrome c from mitochondria Inferred from mutant phenotype. Source: MGI
Cellular component	cytosol Inferred from direct assay. Source: MGI mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCL2L2	Q92843	1	EBI-783400,EBI-707714	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 195

195

BH3-interacting domain death agonist

PRO_0000143102

Chain

61 – 195

135

BH3-interacting domain death agonist p15

PRO_0000223236

Chain

76 – 195

120

BH3-interacting domain death agonist p13

PRO_0000223235

Chain

99 – 195

97

BH3-interacting domain death agonist p11

PRO_0000223234

Regions

Motif

87 – 100

14

BH3

Sites

Site

60 – 61

2

Cleavage

Site

75 – 76

2

Cleavage

Site

98 – 99

2

Cleavage

Amino acid modifications

Modified residue

78

1

Phosphoserine By similarity

Experimental info

Mutagenesis

98

1

D → A: Loss of proteolytical cleavage leading to the production of p11 BID. Ref.4 Ref.1

Sequence conflict

14

1

E → K in AAC71064. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

195

Helix Strand Turn

Details...

Helix

15 – 27

13

Beta strand

28 – 30

3

Helix

32 – 39

8

Helix

79 – 99

21

Beta strand

103 – 105

3

Helix

106 – 113

8

Beta strand

116 – 122

7

Helix

125 – 137

13

Helix

142 – 162

21

Helix

167 – 180

14

Helix

185 – 192

8

Sequences

	Sequence		Length	Mass (Da)
	P70444-1 [UniParc]. Last modified February 21, 2006. Version 2. Checksum: 52F412714FB867F3	FASTA	195	21,952
	10 20 30 40 50 60 MDSEVSNGSG LGAEHITDLL VFGFLQSSGC TRQELEVLGR ELPVQAYWEA DLEDELQTDG 70 80 90 100 110 120 SQASRSFNQG RIEPDSESQE EIIHNIARHL AQIGDEMDHN IQPTLVRQLA AQFMNGSLSE 130 140 150 160 170 180 EDKRNCLAKA LDEVKTAFPR DMENDKAMLI MTMLLAKKVA SHAPSLLRDV FHTTVNFINQ 190 NLFSYVRNLV RNEMD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BID: a novel BH3 domain-only death agonist." Wang K., Yin X.-M., Chao D.T., Milliman C.L., Korsmeyer S.J. Genes Dev. 10:2859-2869(1996) [PubMed: 8918887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF BH3 MOTIF. Tissue: T-cell.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland.
[4]	"Caspase cleaved BID targets mitochondria and is required for cytochrome c release, while BCL-XL prevents this release but not tumor necrosis factor-R1/Fas death." Gross A., Yin X.-M., Wang K., Wei M.C., Jockel J., Milliman C., Erdjument-Bromage H., Tempst P., Korsmeyer S.J. J. Biol. Chem. 274:1156-1163(1999) [PubMed: 9873064] [Abstract] Cited for: CLEAVAGE SITES, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-98, FUNCTION.
[5]	"Solution structure of the proapoptotic molecule BID: a structural basis for apoptotic agonists and antagonists." McDonnell J.M., Fushman D., Milliman C.L., Korsmeyer S.J., Cowburn D. Cell 96:625-634(1999) [PubMed: 10089878] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U75506 mRNA. Translation: AAC71064.1.
AK045731 mRNA. Translation: BAC32475.1.
AK051076 mRNA. Translation: BAC34518.1.
AK052356 mRNA. Translation: BAC34955.1.
AK077657 mRNA. Translation: BAC36932.1.
AK161235 mRNA. Translation: BAE36258.1.
BC002031 mRNA. Translation: AAH02031.1.

IPI

IPI00308086.

RefSeq

NP_031570.2.

UniGene

Mm.235081

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DDB	NMR	-	A	1-195	[»]
2VOI	X-ray	2.10	B	76-109	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P70444. 4 interactions.

PTM databases

PhosphoSite

P70444.

Proteomic databases

PRIDE

P70444.

Genome annotation databases

Ensembl

ENSMUSG00000004446. Mus musculus. [Contig view]

GeneID

12122.

KEGG

mmu:12122.

Organism-specific databases

MGI

MGI:108093. Bid.

Phylogenomic databases

HOGENOM

P70444.

HOVERGEN

P70444.

OMA

P70444. YDDELQT.

Gene expression databases

ArrayExpress

P70444.

Bgee

P70444.

CleanEx

MM_BID.

GermOnline

ENSMUSG00000004446. Mus musculus.

Family and domain databases

InterPro

IPR000712. Bcl2_BH.
IPR010479. BID.
[Graphical view]

Pfam

PF06393. BID. 1 hit.
[Graphical view]

PIRSF

PIRSF038018. BID. 1 hit.

PROSITE

PS01259. BH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

280439.

PMAP-CutDB

P70444.

SOURCE

Search...

Entry information

Entry name

BID_MOUSE

Accession

Primary (citable) accession number: P70444
Secondary accession number(s): Q99M39

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 21, 2006
Last modified:	June 16, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)