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Protein

Ena/VASP-like protein

Gene

Evl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.2 Publications

GO - Molecular functioni

  • profilin binding Source: UniProtKB
  • SH3 domain binding Source: UniProtKB

GO - Biological processi

  • actin filament-based movement Source: UniProtKB
  • actin nucleation Source: UniProtKB
  • actin polymerization or depolymerization Source: UniProtKB
  • axon guidance Source: UniProtKB
  • barbed-end actin filament capping Source: UniProtKB
  • negative regulation of epithelial cell migration Source: MGI
  • negative regulation of ruffle assembly Source: MGI
  • platelet activation Source: UniProtKB
  • positive regulation of actin filament polymerization Source: UniProtKB
  • positive regulation of stress fiber assembly Source: MGI
  • protein homotetramerization Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-MMU-202433. Generation of second messenger molecules.
R-MMU-376176. Signaling by Robo receptor.
R-MMU-5663220. RHO GTPases Activate Formins.

Names & Taxonomyi

Protein namesi
Recommended name:
Ena/VASP-like protein
Alternative name(s):
Ena/vasodilator-stimulated phosphoprotein-like
Gene namesi
Name:Evl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1194884. Evl.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoskeleton Source: UniProtKB-SubCell
  • focal adhesion Source: UniProtKB
  • lamellipodium Source: UniProtKB
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000871051 – 414Ena/VASP-like proteinAdd BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei130PhosphoserineBy similarity1
Modified residuei244PhosphoserineBy similarity1
Modified residuei257PhosphoserineBy similarity1
Modified residuei302PhosphoserineBy similarity1
Modified residuei304PhosphoserineBy similarity1
Modified residuei327PhosphoserineBy similarity1
Modified residuei329PhosphoserineCombined sources1
Modified residuei339PhosphoserineBy similarity1
Modified residuei347PhosphoserineBy similarity1
Modified residuei352PhosphoserineBy similarity1
Modified residuei367PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by PKA; phosphorylation abolishes binding to SH3 domains of ABL and SRC.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP70429.
PaxDbiP70429.
PRIDEiP70429.

PTM databases

iPTMnetiP70429.
PhosphoSitePlusiP70429.

Expressioni

Tissue specificityi

Highest expression in thymus and spleen (at protein level). Low levels in placenta, ovary, testis, fat and lung (at protein level). Isoform 1 and isoform 2 are expressed in cortical neurons and glial cells.2 Publications

Developmental stagei

At an early stage, highly expressed in the branchial and pharyngeal arches, but not in the brain. Expression in the brain starts at 15 dpc (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000021262.
CleanExiMM_EVL.
ExpressionAtlasiP70429. baseline and differential.
GenevisibleiP70429. MM.

Interactioni

Subunit structurei

Homotetramer (By similarity). Binds to the SH3 domains of ABL1, LYN and SRC. Also binds to profilin, with preference for isoform IIa of PFN2, and the WW domain of APBB1/FE65. Binds to SEMA6A. Interacts, via the Pro-rich region, with the C-terminal SH3 domain of DNMBP. Interacts with RAPH1. Binds, via the EVH1 domain, the Pro-rich domain of ZYX (By similarity). Binds, via the EVH1 domain, the Pro-rich domain of Listeria monocytogenes actA.By similarity5 Publications

GO - Molecular functioni

  • profilin binding Source: UniProtKB
  • SH3 domain binding Source: UniProtKB

Protein-protein interaction databases

BioGridi199547. 2 interactors.
DIPiDIP-40886N.
IntActiP70429. 1 interactor.
MINTiMINT-142769.
STRINGi10090.ENSMUSP00000021689.

Structurei

Secondary structure

1414
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 17Combined sources14
Turni18 – 21Combined sources4
Beta strandi22 – 25Combined sources4
Beta strandi34 – 41Combined sources8
Turni42 – 45Combined sources4
Beta strandi46 – 52Combined sources7
Turni54 – 56Combined sources3
Beta strandi59 – 64Combined sources6
Beta strandi70 – 75Combined sources6
Beta strandi78 – 82Combined sources5
Beta strandi87 – 93Combined sources7
Helixi95 – 111Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QC6X-ray2.60A/B1-130[»]
ProteinModelPortaliP70429.
SMRiP70429.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70429.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 112WH1PROSITE-ProRule annotationAdd BLAST112

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 411EVH2Add BLAST192
Regioni220 – 240EVH2 block AAdd BLAST21
Regioni263 – 280EVH2 block BAdd BLAST18
Regioni377 – 411EVH2 block CAdd BLAST35

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi229 – 232KLKR4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi160 – 204Pro-richAdd BLAST45

Domaini

The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.

Sequence similaritiesi

Belongs to the Ena/VASP family.Curated
Contains 1 WH1 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiENOG410KD2J. Eukaryota.
ENOG410YM7V. LUCA.
GeneTreeiENSGT00730000110272.
HOGENOMiHOG000013015.
HOVERGENiHBG006655.
InParanoidiP70429.
OMAiQHRQESL.
OrthoDBiEOG091G0QTE.
PhylomeDBiP70429.
TreeFamiTF321411.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR017354. Vasodilator_phosphoprotein.
IPR014885. VASP_tetra.
IPR000697. WH1/EVH1_dom.
[Graphical view]
PfamiPF08776. VASP_tetra. 1 hit.
PF00568. WH1. 1 hit.
[Graphical view]
PIRSFiPIRSF038010. Vasodilator_Phospo. 1 hit.
SMARTiSM00461. WH1. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50229. WH1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: P70429-1) [UniParc]FASTAAdd to basket
Also known as: EVL-I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEQSICQAR ASVMVYDDTS KKWVPIKPGQ QGFSRINIYH NTASSTFRVV
60 70 80 90 100
GVKLQDQQVV INYSIVKGLK YNQATPTFHQ WRDARQVYGL NFASKEEATT
110 120 130 140 150
FSNAMLFALN IMNSQEGGPS TQRQVQNGPS PEEMDIQRRQ VMEQQHRQES
160 170 180 190 200
LERRISATGP ILPPGHPSSA ASTTLSCSGP PPPPPPPVPP PPTGSTPPPP
210 220 230 240 250
PPLPAGGAQG TNHDESSASG LAAALAGAKL RRVQRPEDAS GGSSPSGTSK
260 270 280 290 300
SDANRASSGG GGGGLMEEMN KLLAKRRKAA SQTDKPADRK EDESQTEDPS
310 320 330 340 350
TSPSPGTRAT SQPPNSSEAG RKPWERSNSV EKPVSSLLSR TPSVAKSPEA
360 370 380 390 400
KSPLQSQPHS RVKPAGSVND VGLDALDLDR MKQEILEEVV RELHKVKEEI
410
IDAIRQELSG ISTT
Length:414
Mass (Da):44,337
Last modified:November 15, 2002 - v2
Checksum:i146A018BCD6CA370
GO
Isoform 1 (identifier: P70429-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     339-359: Missing.

Show »
Length:393
Mass (Da):42,123
Checksum:iBBD63FB013ADBE00
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004045339 – 359Missing in isoform 1. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72519 mRNA. Translation: AAC52862.1.
AF279662 mRNA. Translation: AAG23653.1.
CCDSiCCDS26161.1. [P70429-2]
CCDS49167.1. [P70429-1]
RefSeqiNP_001156866.1. NM_001163394.1. [P70429-1]
NP_031991.3. NM_007965.3. [P70429-2]
UniGeneiMm.238841.

Genome annotation databases

EnsembliENSMUST00000021689; ENSMUSP00000021689; ENSMUSG00000021262. [P70429-1]
ENSMUST00000077735; ENSMUSP00000076916; ENSMUSG00000021262. [P70429-2]
GeneIDi14026.
KEGGimmu:14026.
UCSCiuc007ozv.1. mouse. [P70429-2]
uc007ozw.1. mouse. [P70429-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72519 mRNA. Translation: AAC52862.1.
AF279662 mRNA. Translation: AAG23653.1.
CCDSiCCDS26161.1. [P70429-2]
CCDS49167.1. [P70429-1]
RefSeqiNP_001156866.1. NM_001163394.1. [P70429-1]
NP_031991.3. NM_007965.3. [P70429-2]
UniGeneiMm.238841.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QC6X-ray2.60A/B1-130[»]
ProteinModelPortaliP70429.
SMRiP70429.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199547. 2 interactors.
DIPiDIP-40886N.
IntActiP70429. 1 interactor.
MINTiMINT-142769.
STRINGi10090.ENSMUSP00000021689.

PTM databases

iPTMnetiP70429.
PhosphoSitePlusiP70429.

Proteomic databases

MaxQBiP70429.
PaxDbiP70429.
PRIDEiP70429.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021689; ENSMUSP00000021689; ENSMUSG00000021262. [P70429-1]
ENSMUST00000077735; ENSMUSP00000076916; ENSMUSG00000021262. [P70429-2]
GeneIDi14026.
KEGGimmu:14026.
UCSCiuc007ozv.1. mouse. [P70429-2]
uc007ozw.1. mouse. [P70429-1]

Organism-specific databases

CTDi51466.
MGIiMGI:1194884. Evl.

Phylogenomic databases

eggNOGiENOG410KD2J. Eukaryota.
ENOG410YM7V. LUCA.
GeneTreeiENSGT00730000110272.
HOGENOMiHOG000013015.
HOVERGENiHBG006655.
InParanoidiP70429.
OMAiQHRQESL.
OrthoDBiEOG091G0QTE.
PhylomeDBiP70429.
TreeFamiTF321411.

Enzyme and pathway databases

ReactomeiR-MMU-202433. Generation of second messenger molecules.
R-MMU-376176. Signaling by Robo receptor.
R-MMU-5663220. RHO GTPases Activate Formins.

Miscellaneous databases

EvolutionaryTraceiP70429.
PROiP70429.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021262.
CleanExiMM_EVL.
ExpressionAtlasiP70429. baseline and differential.
GenevisibleiP70429. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR017354. Vasodilator_phosphoprotein.
IPR014885. VASP_tetra.
IPR000697. WH1/EVH1_dom.
[Graphical view]
PfamiPF08776. VASP_tetra. 1 hit.
PF00568. WH1. 1 hit.
[Graphical view]
PIRSFiPIRSF038010. Vasodilator_Phospo. 1 hit.
SMARTiSM00461. WH1. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50229. WH1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEVL_MOUSE
AccessioniPrimary (citable) accession number: P70429
Secondary accession number(s): Q9ERU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: November 2, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Required to transform actin polymerization into active movement for the propulsive force of Listeria monocytogenes.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.