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Protein

Ena/VASP-like protein

Gene

Evl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.2 Publications

Miscellaneous

Required to transform actin polymerization into active movement for the propulsive force of Listeria monocytogenes.

GO - Molecular functioni

  • actin binding Source: UniProtKB-KW
  • profilin binding Source: UniProtKB
  • SH3 domain binding Source: UniProtKB

GO - Biological processi

  • actin filament-based movement Source: UniProtKB
  • actin nucleation Source: UniProtKB
  • actin polymerization or depolymerization Source: UniProtKB
  • axon guidance Source: UniProtKB
  • barbed-end actin filament capping Source: UniProtKB
  • cellular response to interferon-gamma Source: MGI
  • negative regulation of epithelial cell migration Source: MGI
  • negative regulation of ruffle assembly Source: MGI
  • platelet activation Source: UniProtKB
  • positive regulation of actin filament polymerization Source: UniProtKB
  • positive regulation of stress fiber assembly Source: MGI
  • protein homotetramerization Source: InterPro

Keywordsi

Molecular functionActin-binding

Enzyme and pathway databases

ReactomeiR-MMU-376176 Signaling by ROBO receptors
R-MMU-5663220 RHO GTPases Activate Formins

Names & Taxonomyi

Protein namesi
Recommended name:
Ena/VASP-like protein
Alternative name(s):
Ena/vasodilator-stimulated phosphoprotein-like
Gene namesi
Name:Evl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1194884 Evl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000871051 – 414Ena/VASP-like proteinAdd BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei130PhosphoserineBy similarity1
Modified residuei244PhosphoserineBy similarity1
Modified residuei257PhosphoserineBy similarity1
Modified residuei302PhosphoserineBy similarity1
Modified residuei304PhosphoserineBy similarity1
Modified residuei327PhosphoserineBy similarity1
Modified residuei329PhosphoserineCombined sources1
Modified residuei339PhosphoserineBy similarity1
Modified residuei347PhosphoserineBy similarity1
Modified residuei352PhosphoserineBy similarity1
Modified residuei367PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by PKA; phosphorylation abolishes binding to SH3 domains of ABL and SRC.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP70429
PaxDbiP70429
PRIDEiP70429

PTM databases

iPTMnetiP70429
PhosphoSitePlusiP70429

Expressioni

Tissue specificityi

Highest expression in thymus and spleen (at protein level). Low levels in placenta, ovary, testis, fat and lung (at protein level). Isoform 1 and isoform 2 are expressed in cortical neurons and glial cells.2 Publications

Developmental stagei

At an early stage, highly expressed in the branchial and pharyngeal arches, but not in the brain. Expression in the brain starts at 15 dpc (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000021262
CleanExiMM_EVL
ExpressionAtlasiP70429 baseline and differential
GenevisibleiP70429 MM

Interactioni

Subunit structurei

Homotetramer (By similarity). Binds to the SH3 domains of ABL1, LYN and SRC (PubMed:10945997). Also binds to profilin, with preference for isoform IIa of PFN2, and the WW domain of APBB1/FE65 (PubMed:10945997). Binds to SEMA6A (PubMed:10993894). Interacts, via the Pro-rich region, with the C-terminal SH3 domain of DNMBP (PubMed:14506234). Interacts with RAPH1 (By similarity). Binds, via the EVH1 domain, the Pro-rich domain of Listeria monocytogenes actA (PubMed:10087267). Binds, via the EVH1 domain, the Pro-rich domain of ZYX. Interacts with FYB1. Interacts with ZDHHC17 (By similarity).By similarity5 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB-KW
  • profilin binding Source: UniProtKB
  • SH3 domain binding Source: UniProtKB

Protein-protein interaction databases

BioGridi199547, 2 interactors
DIPiDIP-40886N
ELMiP70429
IntActiP70429, 2 interactors
STRINGi10090.ENSMUSP00000021689

Structurei

Secondary structure

1414
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 17Combined sources14
Turni18 – 21Combined sources4
Beta strandi22 – 25Combined sources4
Beta strandi34 – 41Combined sources8
Turni42 – 45Combined sources4
Beta strandi46 – 52Combined sources7
Turni54 – 56Combined sources3
Beta strandi59 – 64Combined sources6
Beta strandi70 – 75Combined sources6
Beta strandi78 – 82Combined sources5
Beta strandi87 – 93Combined sources7
Helixi95 – 111Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QC6X-ray2.60A/B1-130[»]
ProteinModelPortaliP70429
SMRiP70429
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70429

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 112WH1PROSITE-ProRule annotationAdd BLAST112

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 411EVH2Add BLAST192
Regioni220 – 240EVH2 block AAdd BLAST21
Regioni263 – 280EVH2 block BAdd BLAST18
Regioni340 – 360Required for interaction with ZDHHC17By similarityAdd BLAST21
Regioni377 – 411EVH2 block CAdd BLAST35

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi229 – 232KLKR4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi160 – 204Pro-richAdd BLAST45

Domaini

The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.

Sequence similaritiesi

Belongs to the Ena/VASP family.Curated

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiENOG410KD2J Eukaryota
ENOG410YM7V LUCA
GeneTreeiENSGT00730000110272
HOGENOMiHOG000013015
HOVERGENiHBG006655
InParanoidiP70429
OMAiSTQRQVQ
OrthoDBiEOG091G0QTE
PhylomeDBiP70429
TreeFamiTF321411

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR034319 ENA/VASP-like_protein
IPR011993 PH-like_dom_sf
IPR017354 VASP/EVL
IPR038023 VASP_sf
IPR014885 VASP_tetra
IPR000697 WH1/EVH1_dom
PANTHERiPTHR11202:SF4 PTHR11202:SF4, 1 hit
PfamiView protein in Pfam
PF08776 VASP_tetra, 1 hit
PF00568 WH1, 1 hit
PIRSFiPIRSF038010 Vasodilator_Phospo, 1 hit
SMARTiView protein in SMART
SM00461 WH1, 1 hit
SUPFAMiSSF118370 SSF118370, 1 hit
PROSITEiView protein in PROSITE
PS50229 WH1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: P70429-1) [UniParc]FASTAAdd to basket
Also known as: EVL-I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEQSICQAR ASVMVYDDTS KKWVPIKPGQ QGFSRINIYH NTASSTFRVV
60 70 80 90 100
GVKLQDQQVV INYSIVKGLK YNQATPTFHQ WRDARQVYGL NFASKEEATT
110 120 130 140 150
FSNAMLFALN IMNSQEGGPS TQRQVQNGPS PEEMDIQRRQ VMEQQHRQES
160 170 180 190 200
LERRISATGP ILPPGHPSSA ASTTLSCSGP PPPPPPPVPP PPTGSTPPPP
210 220 230 240 250
PPLPAGGAQG TNHDESSASG LAAALAGAKL RRVQRPEDAS GGSSPSGTSK
260 270 280 290 300
SDANRASSGG GGGGLMEEMN KLLAKRRKAA SQTDKPADRK EDESQTEDPS
310 320 330 340 350
TSPSPGTRAT SQPPNSSEAG RKPWERSNSV EKPVSSLLSR TPSVAKSPEA
360 370 380 390 400
KSPLQSQPHS RVKPAGSVND VGLDALDLDR MKQEILEEVV RELHKVKEEI
410
IDAIRQELSG ISTT
Length:414
Mass (Da):44,337
Last modified:November 15, 2002 - v2
Checksum:i146A018BCD6CA370
GO
Isoform 1 (identifier: P70429-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     339-359: Missing.

Show »
Length:393
Mass (Da):42,123
Checksum:iBBD63FB013ADBE00
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004045339 – 359Missing in isoform 1. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72519 mRNA Translation: AAC52862.1
AF279662 mRNA Translation: AAG23653.1
CCDSiCCDS26161.1 [P70429-2]
CCDS49167.1 [P70429-1]
RefSeqiNP_001156866.1, NM_001163394.1 [P70429-1]
NP_031991.3, NM_007965.3 [P70429-2]
UniGeneiMm.238841

Genome annotation databases

EnsembliENSMUST00000021689; ENSMUSP00000021689; ENSMUSG00000021262 [P70429-1]
ENSMUST00000077735; ENSMUSP00000076916; ENSMUSG00000021262 [P70429-2]
GeneIDi14026
KEGGimmu:14026
UCSCiuc007ozv.1 mouse [P70429-2]
uc007ozw.1 mouse [P70429-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEVL_MOUSE
AccessioniPrimary (citable) accession number: P70429
Secondary accession number(s): Q9ERU8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: March 28, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health