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P70428

- EXT2_MOUSE

UniProt

P70428 - EXT2_MOUSE

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Protein

Exostosin-2

Gene

Ext2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

Manganese.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei490 – 4901SubstrateBy similarity
Metal bindingi540 – 5401Manganese; catalyticBy similarity
Binding sitei569 – 5691SubstrateBy similarity
Active sitei628 – 6281By similarity

GO - Molecular functioni

  1. acetylglucosaminyltransferase activity Source: BHF-UCL
  2. glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Source: BHF-UCL
  3. glucuronosyltransferase activity Source: BHF-UCL
  4. heparan sulfate N-acetylglucosaminyltransferase activity Source: BHF-UCL
  5. N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Source: BHF-UCL
  6. protein homodimerization activity Source: BHF-UCL

GO - Biological processi

  1. cell differentiation Source: MGI
  2. glycosaminoglycan biosynthetic process Source: InterPro
  3. heparan sulfate proteoglycan biosynthetic process Source: MGI
  4. heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: Ensembl
  5. mesoderm formation Source: MGI
  6. ossification Source: Ensembl
  7. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198654. HS-GAG biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-2 (EC:2.4.1.224, EC:2.4.1.225)
Alternative name(s):
Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
Multiple exostoses protein 2 homolog
Gene namesi
Name:Ext2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:108050. Ext2.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: BHF-UCL
  2. endoplasmic reticulum membrane Source: BHF-UCL
  3. extracellular vesicular exosome Source: Ensembl
  4. Golgi apparatus Source: BHF-UCL
  5. Golgi membrane Source: BHF-UCL
  6. integral component of membrane Source: UniProtKB-KW
  7. intrinsic component of endoplasmic reticulum membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718Exostosin-2PRO_0000149652Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi626 ↔ 676By similarity
Glycosylationi637 – 6371N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP70428.
PaxDbiP70428.
PRIDEiP70428.

PTM databases

PhosphoSiteiP70428.

Expressioni

Tissue specificityi

Heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.

Developmental stagei

Expressed in early stages of embryonic development.

Gene expression databases

BgeeiP70428.
CleanExiMM_EXT2.
ExpressionAtlasiP70428. baseline and differential.
GenevestigatoriP70428.

Interactioni

Subunit structurei

Interacts with GALNT5 (By similarity). Forms a homo/hetero-oligomeric complex with EXT1.By similarity

Protein-protein interaction databases

DIPiDIP-29858N.

Structurei

3D structure databases

ProteinModelPortaliP70428.
SMRiP70428. Positions 456-690.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini47 – 718672LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei26 – 4621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni517 – 5226Substrate bindingBy similarity
Regioni538 – 5403Substrate bindingBy similarity
Regioni624 – 6285Substrate bindingBy similarity
Regioni662 – 67312Substrate bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG272619.
GeneTreeiENSGT00550000074496.
HOVERGENiHBG101211.
InParanoidiP70428.
KOiK02367.
OMAiQFGYEVW.
OrthoDBiEOG789C9T.
TreeFamiTF314231.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004263. Exostosin.
IPR027673. Exostosin-2.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11062. PTHR11062. 1 hit.
PTHR11062:SF6. PTHR11062:SF6. 1 hit.
PfamiPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P70428-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCASVKSNIR GPALIPRMKT KHRIYYVTLF SIVLLGLIAT GMFQFWPHSI
60 70 80 90 100
ESSSDGGVEK RSIREVPVVR LPTDSPIPER GDLSCRMHTC FDVYRCGFNP
110 120 130 140 150
KNKIKVYIYP LKKYVDDAGV PVSSAISREY NELLTAISDS DYYTDDINRA
160 170 180 190 200
CLFVPSIDVL NQNPLRIKET AQALAQLSRW DRGTNHLLFN MLPGAPPDYN
210 220 230 240 250
TALDVPRDRA LLAGGGFSTW TYRQGYDVSI PVFSPLSAEM ALPEKAPGPR
260 270 280 290 300
RYFLLSSQMA IHPEYREELE ALQAKHQESV LVLDKCTNLS EGVLSVRKRC
310 320 330 340 350
HQHQVFDYPQ VLQEATFCTV LRGARLGQAV LSDVLQAGCV PVVIADSYIL
360 370 380 390 400
PFSEVLDWKR ASVVVPEEKM SDVYSILQNI PQRQIEEMQR QARWFWEAYF
410 420 430 440 450
QSIKAIALAT LQIINDRIYP YAAISYEEWN DPPAVKWASV SNPLFLPLIP
460 470 480 490 500
PQSQGFTAIV LTYDRVESLF RVITEVSKVP SLSKLLVVWN NQNKNPPEES
510 520 530 540 550
LWPKIRVPLK VVRTAENKLS NRFFPYDEIE TEAVLAIDDD IIMLTSDELQ
560 570 580 590 600
FGYEVWREFP DRLVGYPGRL HLWDHEMNKW KYESEWTNEV SMVLTGAAFY
610 620 630 640 650
HKYFNYLYTY KMPGDIKNWV DTHMNCEDIA MNFLVANVTG KAVIKVTPRK
660 670 680 690 700
KFKCPECTAI DGLSLDQTHM VERSECINKF ASVFGTMPLK VVEHRADPVL
710
YKDDFPEKLK SFPNIGSL
Length:718
Mass (Da):82,064
Last modified:July 27, 2011 - v2
Checksum:i45D947BF6678378B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621S → T in AAC53143. (PubMed:9110175)Curated
Sequence conflicti160 – 1601L → V in AAC53143. (PubMed:9110175)Curated
Sequence conflicti323 – 3231G → R in AAC53143. (PubMed:9110175)Curated
Sequence conflicti355 – 3551V → I in AAC53143. (PubMed:9110175)Curated
Sequence conflicti360 – 3601R → K in AAC53143. (PubMed:9110175)Curated
Sequence conflicti389 – 3891Q → H in AAC53143. (PubMed:9110175)Curated
Sequence conflicti622 – 6221T → A in AAC53143. (PubMed:9110175)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U72141 mRNA. Translation: AAB17006.1.
U67837 mRNA. Translation: AAC53143.1.
AK164342 mRNA. Translation: BAE37749.1.
AL732493, AL732472 Genomic DNA. Translation: CAM22097.1.
AL732472, AL732493 Genomic DNA. Translation: CAM23881.1.
CH466519 Genomic DNA. Translation: EDL27629.1.
BC006597 mRNA. Translation: AAH06597.1.
CCDSiCCDS16456.1.
RefSeqiNP_034293.2. NM_010163.3.
XP_006498796.1. XM_006498733.1.
UniGeneiMm.4336.

Genome annotation databases

EnsembliENSMUST00000028623; ENSMUSP00000028623; ENSMUSG00000027198.
GeneIDi14043.
KEGGimmu:14043.
UCSCiuc008lgf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U72141 mRNA. Translation: AAB17006.1 .
U67837 mRNA. Translation: AAC53143.1 .
AK164342 mRNA. Translation: BAE37749.1 .
AL732493 , AL732472 Genomic DNA. Translation: CAM22097.1 .
AL732472 , AL732493 Genomic DNA. Translation: CAM23881.1 .
CH466519 Genomic DNA. Translation: EDL27629.1 .
BC006597 mRNA. Translation: AAH06597.1 .
CCDSi CCDS16456.1.
RefSeqi NP_034293.2. NM_010163.3.
XP_006498796.1. XM_006498733.1.
UniGenei Mm.4336.

3D structure databases

ProteinModelPortali P70428.
SMRi P70428. Positions 456-690.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29858N.

Protein family/group databases

CAZyi GT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

PTM databases

PhosphoSitei P70428.

Proteomic databases

MaxQBi P70428.
PaxDbi P70428.
PRIDEi P70428.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028623 ; ENSMUSP00000028623 ; ENSMUSG00000027198 .
GeneIDi 14043.
KEGGi mmu:14043.
UCSCi uc008lgf.2. mouse.

Organism-specific databases

CTDi 2132.
MGIi MGI:108050. Ext2.

Phylogenomic databases

eggNOGi NOG272619.
GeneTreei ENSGT00550000074496.
HOVERGENi HBG101211.
InParanoidi P70428.
KOi K02367.
OMAi QFGYEVW.
OrthoDBi EOG789C9T.
TreeFami TF314231.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_198654. HS-GAG biosynthesis.

Miscellaneous databases

ChiTaRSi EXT2. mouse.
NextBioi 284982.
PROi P70428.
SOURCEi Search...

Gene expression databases

Bgeei P70428.
CleanExi MM_EXT2.
ExpressionAtlasi P70428. baseline and differential.
Genevestigatori P70428.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR004263. Exostosin.
IPR027673. Exostosin-2.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR11062. PTHR11062. 1 hit.
PTHR11062:SF6. PTHR11062:SF6. 1 hit.
Pfami PF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the murine homolog of the human EXT2 multiple exostoses gene."
    Stickens D.J., Evans G.A.
    Biochem. Mol. Med. 61:16-21(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The structure of the human multiple exostoses 2 gene and characterization of homologs in mouse and Caenorhabditis elegans."
    Clines G.A., Ashley J.A., Shah S., Lovett M.
    Genome Res. 7:359-367(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Spinal ganglion.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  7. "The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate."
    McCormick C., Duncan G., Goutsos K.T., Tufaro F.
    Proc. Natl. Acad. Sci. U.S.A. 97:668-673(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiEXT2_MOUSE
AccessioniPrimary (citable) accession number: P70428
Secondary accession number(s): P70395, Q3TPI7, Q923D6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3