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Reviewed, UniProtKB/Swiss-Prot P70428 (EXT2_MOUSE)

Last modified June 16, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Exostosin-2
    EC=2.4.1.224
    EC=2.4.1.225
Alternative name(s):
    Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
    Multiple exostoses protein 2 homolog
Gene names
Name: Ext2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length718 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor.

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.

UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Interacts with GALNT5 By similarity. Forms a homo/hetero-oligomeric complex with EXT1.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Note: The EXT1/EXT2 complex is localized in the Golgi apparatus.

Tissue specificity

Heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.

Developmental stage

Expressed in early stages of embryonic development.

Sequence similarities

Belongs to the glycosyltransferase 47 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 718718Exostosin-2
PRO_0000149652

Regions

Topological domain1 – 2525Cytoplasmic Potential
Transmembrane26 – 4621Signal-anchor for type II membrane protein Potential
Topological domain47 – 718672Lumenal Potential

Amino acid modifications

Glycosylation2881N-linked (GlcNAc...) Potential
Glycosylation6371N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict621S → T in AAC53143. Ref.2
Sequence conflict1601L → V in AAC53143. Ref.2
Sequence conflict3231R → G in AAH06597. Ref.3
Sequence conflict3551V → I in AAC53143. Ref.2
Sequence conflict3601K → R in AAH06597. Ref.3
Sequence conflict3891Q → H in AAC53143. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P70428-1 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: C2E765393A7F7ABD

FASTA71882,105
        10         20         30         40         50         60 
MCASVKSNIR GPALIPRMKT KHRIYYVTLF SIVLLGLIAT GMFQFWPHSI ESSSDGGVEK 

        70         80         90        100        110        120 
RSIREVPVVR LPTDSPIPER GDLSCRMHTC FDVYRCGFNP KNKIKVYIYP LKKYVDDAGV 

       130        140        150        160        170        180 
PVSSAISREY NELLTAISDS DYYTDDINRA CLFVPSIDVL NQNPLRIKET AQALAQLSRW 

       190        200        210        220        230        240 
DRGTNHLLFN MLPGAPPDYN TALDVPRDRA LLAGGGFSTW TYRQGYDVSI PVFSPLSAEM 

       250        260        270        280        290        300 
ALPEKAPGPR RYFLLSSQMA IHPEYREELE ALQAKHQESV LVLDKCTNLS EGVLSVRKRC 

       310        320        330        340        350        360 
HQHQVFDYPQ VLQEATFCTV LRRARLGQAV LSDVLQAGCV PVVIADSYIL PFSEVLDWKK 

       370        380        390        400        410        420 
ASVVVPEEKM SDVYSILQNI PQRQIEEMQR QARWFWEAYF QSIKAIALAT LQIINDRIYP 

       430        440        450        460        470        480 
YAAISYEEWN DPPAVKWASV SNPLFLPLIP PQSQGFTAIV LTYDRVESLF RVITEVSKVP 

       490        500        510        520        530        540 
SLSKLLVVWN NQNKNPPEES LWPKIRVPLK VVRTAENKLS NRFFPYDEIE TEAVLAIDDD 

       550        560        570        580        590        600 
IIMLTSDELQ FGYEVWREFP DRLVGYPGRL HLWDHEMNKW KYESEWTNEV SMVLTGAAFY 

       610        620        630        640        650        660 
HKYFNYLYTY KMPGDIKNWV DAHMNCEDIA MNFLVANVTG KAVIKVTPRK KFKCPECTAI 

       670        680        690        700        710 
DGLSLDQTHM VERSECINKF ASVFGTMPLK VVEHRADPVL YKDDFPEKLK SFPNIGSL 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of the murine homolog of the human EXT2 multiple exostoses gene."
Stickens D.J., Evans G.A.
Biochem. Mol. Med. 61:16-21(1997) [PubMed: 9232192] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The structure of the human multiple exostoses 2 gene and characterization of homologs in mouse and Caenorhabditis elegans."
Clines G.A., Ashley J.A., Shah S., Lovett M.
Genome Res. 7:359-367(1997) [PubMed: 9110175] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary gland.
[4]"The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate."
McCormick C., Duncan G., Goutsos K.T., Tufaro F.
Proc. Natl. Acad. Sci. U.S.A. 97:668-673(2000) [PubMed: 10639137] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

U72141 mRNA. Translation: AAB17006.1.
U67837 mRNA. Translation: AAC53143.1.
BC006597 mRNA. Translation: AAH06597.1.
IPIIPI00308075.
UniGeneMm.4336

3D structure databases

HSSPHSSP built from PDB template 1OMX based on UniProtKB Q9ES89.
ModBaseSearch...

Protein family/group databases

CAZyGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Genome annotation databases

EnsemblENSMUSG00000027198. Mus musculus. [Contig view]
KEGGmmu:14043.

Organism-specific databases

MGIMGI:108050. Ext2.

Phylogenomic databases

HOVERGENP70428.

Enzyme and pathway databases

BRENDA2.4.1.225. 244.

Gene expression databases

ArrayExpressP70428.
BgeeP70428.
CleanExMM_EXT2.
GermOnlineENSMUSG00000027198. Mus musculus.

Family and domain databases

InterProIPR004263. Exostosin.
IPR015338. HexNAc_Trfase_a.
[Graphical view]
PfamPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio284982.
SOURCESearch...

Entry information

Entry nameEXT2_MOUSE
AccessionPrimary (citable) accession number: P70428
Secondary accession number(s): P70395, Q923D6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents