Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Exostosin-2

Gene

Ext2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei490SubstrateBy similarity1
Metal bindingi540Manganese; catalyticBy similarity1
Binding sitei569SubstrateBy similarity1
Active sitei628By similarity1

GO - Molecular functioni

  • acetylglucosaminyltransferase activity Source: BHF-UCL
  • glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Source: BHF-UCL
  • glucuronosyltransferase activity Source: BHF-UCL
  • heparan sulfate N-acetylglucosaminyltransferase activity Source: BHF-UCL
  • metal ion binding Source: UniProtKB-KW
  • N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Source: BHF-UCL
  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: BHF-UCL
  • transferase activity, transferring glycosyl groups Source: MGI

GO - Biological processi

  • cell differentiation Source: MGI
  • cellular polysaccharide biosynthetic process Source: MGI
  • glycosaminoglycan biosynthetic process Source: MGI
  • heparan sulfate proteoglycan biosynthetic process Source: MGI
  • heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: MGI
  • mesoderm formation Source: MGI
  • ossification Source: MGI
  • protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2022928. HS-GAG biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-2 (EC:2.4.1.224, EC:2.4.1.225)
Alternative name(s):
Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
Multiple exostoses protein 2 homolog
Gene namesi
Name:Ext2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:108050. Ext2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
Transmembranei26 – 46Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini47 – 718LumenalSequence analysisAdd BLAST672

GO - Cellular componenti

  • endoplasmic reticulum Source: BHF-UCL
  • endoplasmic reticulum membrane Source: BHF-UCL
  • extracellular exosome Source: MGI
  • Golgi apparatus Source: BHF-UCL
  • Golgi membrane Source: BHF-UCL
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: MGI
  • UDP-N-acetylglucosamine transferase complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001496521 – 718Exostosin-2Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi288N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi626 ↔ 676By similarity
Glycosylationi637N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP70428.
MaxQBiP70428.
PaxDbiP70428.
PeptideAtlasiP70428.
PRIDEiP70428.

PTM databases

PhosphoSitePlusiP70428.

Expressioni

Tissue specificityi

Expressed in heart, brain, spleen, lung, liver, skeletal muscle and testis. Heart shows a high expression.2 Publications

Developmental stagei

Expressed in early stages of embryonic development.

Gene expression databases

BgeeiENSMUSG00000027198.
CleanExiMM_EXT2.
ExpressionAtlasiP70428. baseline and differential.
GenevisibleiP70428. MM.

Interactioni

Subunit structurei

Interacts with GALNT5 (By similarity). Forms a homo/hetero-oligomeric complex with EXT1. Interacts with NDST1 (By similarity).By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: BHF-UCL

Protein-protein interaction databases

DIPiDIP-29858N.
STRINGi10090.ENSMUSP00000028623.

Structurei

3D structure databases

ProteinModelPortaliP70428.
SMRiP70428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni517 – 522Substrate bindingBy similarity6
Regioni538 – 540Substrate bindingBy similarity3
Regioni624 – 628Substrate bindingBy similarity5
Regioni662 – 673Substrate bindingBy similarityAdd BLAST12

Sequence similaritiesi

Belongs to the glycosyltransferase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1021. Eukaryota.
ENOG410XTFH. LUCA.
GeneTreeiENSGT00550000074496.
HOVERGENiHBG101211.
InParanoidiP70428.
KOiK02367.
OMAiQFGYEVW.
OrthoDBiEOG091G02GW.
TreeFamiTF314231.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004263. Exostosin.
IPR027673. Exostosin-2.
IPR015338. EXT_C.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11062:SF93. PTHR11062:SF93. 2 hits.
PfamiPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P70428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCASVKSNIR GPALIPRMKT KHRIYYVTLF SIVLLGLIAT GMFQFWPHSI
60 70 80 90 100
ESSSDGGVEK RSIREVPVVR LPTDSPIPER GDLSCRMHTC FDVYRCGFNP
110 120 130 140 150
KNKIKVYIYP LKKYVDDAGV PVSSAISREY NELLTAISDS DYYTDDINRA
160 170 180 190 200
CLFVPSIDVL NQNPLRIKET AQALAQLSRW DRGTNHLLFN MLPGAPPDYN
210 220 230 240 250
TALDVPRDRA LLAGGGFSTW TYRQGYDVSI PVFSPLSAEM ALPEKAPGPR
260 270 280 290 300
RYFLLSSQMA IHPEYREELE ALQAKHQESV LVLDKCTNLS EGVLSVRKRC
310 320 330 340 350
HQHQVFDYPQ VLQEATFCTV LRGARLGQAV LSDVLQAGCV PVVIADSYIL
360 370 380 390 400
PFSEVLDWKR ASVVVPEEKM SDVYSILQNI PQRQIEEMQR QARWFWEAYF
410 420 430 440 450
QSIKAIALAT LQIINDRIYP YAAISYEEWN DPPAVKWASV SNPLFLPLIP
460 470 480 490 500
PQSQGFTAIV LTYDRVESLF RVITEVSKVP SLSKLLVVWN NQNKNPPEES
510 520 530 540 550
LWPKIRVPLK VVRTAENKLS NRFFPYDEIE TEAVLAIDDD IIMLTSDELQ
560 570 580 590 600
FGYEVWREFP DRLVGYPGRL HLWDHEMNKW KYESEWTNEV SMVLTGAAFY
610 620 630 640 650
HKYFNYLYTY KMPGDIKNWV DTHMNCEDIA MNFLVANVTG KAVIKVTPRK
660 670 680 690 700
KFKCPECTAI DGLSLDQTHM VERSECINKF ASVFGTMPLK VVEHRADPVL
710
YKDDFPEKLK SFPNIGSL
Length:718
Mass (Da):82,064
Last modified:July 27, 2011 - v2
Checksum:i45D947BF6678378B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62S → T in AAC53143 (PubMed:9110175).Curated1
Sequence conflicti160L → V in AAC53143 (PubMed:9110175).Curated1
Sequence conflicti323G → R in AAC53143 (PubMed:9110175).Curated1
Sequence conflicti355V → I in AAC53143 (PubMed:9110175).Curated1
Sequence conflicti360R → K in AAC53143 (PubMed:9110175).Curated1
Sequence conflicti389Q → H in AAC53143 (PubMed:9110175).Curated1
Sequence conflicti622T → A in AAC53143 (PubMed:9110175).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72141 mRNA. Translation: AAB17006.1.
U67837 mRNA. Translation: AAC53143.1.
AK164342 mRNA. Translation: BAE37749.1.
AL732493, AL732472 Genomic DNA. Translation: CAM22097.1.
AL732472, AL732493 Genomic DNA. Translation: CAM23881.1.
CH466519 Genomic DNA. Translation: EDL27629.1.
BC006597 mRNA. Translation: AAH06597.1.
CCDSiCCDS16456.1.
RefSeqiNP_034293.2. NM_010163.3.
XP_006498796.1. XM_006498733.3.
UniGeneiMm.4336.

Genome annotation databases

EnsembliENSMUST00000028623; ENSMUSP00000028623; ENSMUSG00000027198.
GeneIDi14043.
KEGGimmu:14043.
UCSCiuc008lgf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72141 mRNA. Translation: AAB17006.1.
U67837 mRNA. Translation: AAC53143.1.
AK164342 mRNA. Translation: BAE37749.1.
AL732493, AL732472 Genomic DNA. Translation: CAM22097.1.
AL732472, AL732493 Genomic DNA. Translation: CAM23881.1.
CH466519 Genomic DNA. Translation: EDL27629.1.
BC006597 mRNA. Translation: AAH06597.1.
CCDSiCCDS16456.1.
RefSeqiNP_034293.2. NM_010163.3.
XP_006498796.1. XM_006498733.3.
UniGeneiMm.4336.

3D structure databases

ProteinModelPortaliP70428.
SMRiP70428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29858N.
STRINGi10090.ENSMUSP00000028623.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

PTM databases

PhosphoSitePlusiP70428.

Proteomic databases

EPDiP70428.
MaxQBiP70428.
PaxDbiP70428.
PeptideAtlasiP70428.
PRIDEiP70428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028623; ENSMUSP00000028623; ENSMUSG00000027198.
GeneIDi14043.
KEGGimmu:14043.
UCSCiuc008lgf.2. mouse.

Organism-specific databases

CTDi2132.
MGIiMGI:108050. Ext2.

Phylogenomic databases

eggNOGiKOG1021. Eukaryota.
ENOG410XTFH. LUCA.
GeneTreeiENSGT00550000074496.
HOVERGENiHBG101211.
InParanoidiP70428.
KOiK02367.
OMAiQFGYEVW.
OrthoDBiEOG091G02GW.
TreeFamiTF314231.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-2022928. HS-GAG biosynthesis.

Miscellaneous databases

ChiTaRSiExt2. mouse.
PROiP70428.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027198.
CleanExiMM_EXT2.
ExpressionAtlasiP70428. baseline and differential.
GenevisibleiP70428. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004263. Exostosin.
IPR027673. Exostosin-2.
IPR015338. EXT_C.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11062:SF93. PTHR11062:SF93. 2 hits.
PfamiPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEXT2_MOUSE
AccessioniPrimary (citable) accession number: P70428
Secondary accession number(s): P70395, Q3TPI7, Q923D6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.