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Protein

GTP-binding protein Rit1

Gene

Rit1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation. Involved in ELK1 transactivation through the Ras-MAPK signaling cascade that mediates a wide variety of cellular functions, including cell proliferation, survival, and differentiation (By similarity).By similarity

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 358GTPBy similarity
Nucleotide bindingi75 – 795GTPBy similarity
Nucleotide bindingi134 – 1374GTPBy similarity

GO - Molecular functioni

  • GTP binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein Rit1
Alternative name(s):
Ras-like protein expressed in many tissues
Ras-like without CAAX protein 1
Gene namesi
Name:Rit1
Synonyms:Rit
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:108053. Rit1.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: Ensembl
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 219219GTP-binding protein Rit1PRO_0000082726Add
BLAST

Proteomic databases

MaxQBiP70426.
PaxDbiP70426.
PeptideAtlasiP70426.
PRIDEiP70426.

PTM databases

iPTMnetiP70426.
PhosphoSiteiP70426.

Expressioni

Tissue specificityi

Expressed in many tissues.

Gene expression databases

BgeeiP70426.
CleanExiMM_RIT1.
ExpressionAtlasiP70426. baseline and differential.
GenevisibleiP70426. MM.

Interactioni

Subunit structurei

Interacts with MLLT4, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Strongly interacts with BRAF, but only weakly with RAF1. BARF and RAF1 association is dependent upon the GTP-bound state (By similarity). Interacts with RGL3.By similarity1 Publication

Protein-protein interaction databases

BioGridi202897. 2 interactions.
STRINGi10090.ENSMUSP00000029692.

Structurei

3D structure databases

ProteinModelPortaliP70426.
SMRiP70426. Positions 21-185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP70426.
KOiK07832.
OMAiKSSVWKR.
OrthoDBiEOG7QVM41.
PhylomeDBiP70426.
TreeFamiTF315072.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70426-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGARPIGS SCSSPAALSR EYKLVMLGAG GVGKSAMTMQ FISHRFPEDH
60 70 80 90 100
DPTIEDAYKI RIRIDDEPAN LDILDTAGQA EFTAMRDQYM RAGEGFIICY
110 120 130 140 150
SITDRRSFHE VREFKQLIYR VRRTDDTPVV LVGNKSDLKQ LRQVSKEEGL
160 170 180 190 200
SLAREFSCPF FETSAAYRYY IDDVFHALVR EIRKKEKELV LAMEKKAKPK
210
NSVWKRLKSP FRRKKDSVT
Length:219
Mass (Da):25,169
Last modified:February 1, 1997 - v1
Checksum:i3041ED75190C65AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71205 mRNA. Translation: AAB42215.1.
AK005357 mRNA. Translation: BAB23972.1.
AK014126 mRNA. Translation: BAB29168.1.
BC012694 mRNA. Translation: AAH12694.1.
CCDSiCCDS17484.1.
RefSeqiNP_033095.1. NM_009069.4.
XP_006501223.1. XM_006501160.2.
UniGeneiMm.4009.

Genome annotation databases

EnsembliENSMUST00000029692; ENSMUSP00000029692; ENSMUSG00000028057.
GeneIDi19769.
KEGGimmu:19769.
UCSCiuc008pwh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71205 mRNA. Translation: AAB42215.1.
AK005357 mRNA. Translation: BAB23972.1.
AK014126 mRNA. Translation: BAB29168.1.
BC012694 mRNA. Translation: AAH12694.1.
CCDSiCCDS17484.1.
RefSeqiNP_033095.1. NM_009069.4.
XP_006501223.1. XM_006501160.2.
UniGeneiMm.4009.

3D structure databases

ProteinModelPortaliP70426.
SMRiP70426. Positions 21-185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202897. 2 interactions.
STRINGi10090.ENSMUSP00000029692.

PTM databases

iPTMnetiP70426.
PhosphoSiteiP70426.

Proteomic databases

MaxQBiP70426.
PaxDbiP70426.
PeptideAtlasiP70426.
PRIDEiP70426.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029692; ENSMUSP00000029692; ENSMUSG00000028057.
GeneIDi19769.
KEGGimmu:19769.
UCSCiuc008pwh.2. mouse.

Organism-specific databases

CTDi6016.
MGIiMGI:108053. Rit1.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP70426.
KOiK07832.
OMAiKSSVWKR.
OrthoDBiEOG7QVM41.
PhylomeDBiP70426.
TreeFamiTF315072.

Miscellaneous databases

PROiP70426.
SOURCEiSearch...

Gene expression databases

BgeeiP70426.
CleanExiMM_RIT1.
ExpressionAtlasiP70426. baseline and differential.
GenevisibleiP70426. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rin, a neuron-specific and calmodulin-binding small G-protein, and Rit define a novel subfamily of ras proteins."
    Lee C.H.J., Della N.G., Chew C.E., Zack D.J.
    J. Neurosci. 16:6784-6794(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum and Embryo.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  4. "A novel RalGEF-like protein, RGL3, as a candidate effector for rit and Ras."
    Shao H., Andres D.A.
    J. Biol. Chem. 275:26914-26924(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RGL3.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney.

Entry informationi

Entry nameiRIT1_MOUSE
AccessioniPrimary (citable) accession number: P70426
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: February 1, 1997
Last modified: July 6, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Stimulation of the NGF and EGF receptor signaling pathways results in rapid and prolonged activation.By similarity
Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are very much 10-fold faster than those of most Ras subfamily members.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.