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Protein

GTP-binding protein Rit2

Gene

Rit2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds and exchanges GTP and GDP. Binds and modulates the activation of POU4F1 as gene expression regulator.1 Publication

Miscellaneous

Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are much faster than those of most Ras subfamily members.

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 34GTPBy similarity8
Nucleotide bindingi74 – 78GTPBy similarity5
Nucleotide bindingi133 – 136GTPBy similarity4

GO - Molecular functioni

  • calmodulin binding Source: ParkinsonsUK-UCL
  • chromatin binding Source: ParkinsonsUK-UCL
  • GTPase activity Source: ParkinsonsUK-UCL
  • GTP binding Source: ParkinsonsUK-UCL
  • semaphorin receptor binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein Rit2
Alternative name(s):
Ras-like protein expressed in neurons
Ras-like without CAAX protein 2
Gene namesi
Name:Rit2
Synonyms:Rin, Roc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:108054 Rit2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi34S → N: Dominant negative. Loss of interaction with AFDN, RLF and RALGDS. 1 Publication1
Mutagenesisi52T → A: Loss of interaction with AFDN, RLF and RALGDS; when associated with L-78. 1 Publication1
Mutagenesisi78Q → L: Constitutively active. Dramatic reduction of the rate of GTP hydrolysis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000827281 – 217GTP-binding protein Rit2Add BLAST217

Proteomic databases

MaxQBiP70425
PaxDbiP70425
PRIDEiP70425

PTM databases

PhosphoSitePlusiP70425
SwissPalmiP70425

Expressioni

Tissue specificityi

Expressed in ganglion cell layer (GCL), inner plexiform layer (IPL) and inner nuclear layer (INL) of the retina. Expressed in retinal ganglion cells (RGCs). Expressed in horizontal, bipolar and amacrine cells, but not Mueller glia, of the INL (at protein level). Neuron-specific (PubMed:8824319). Expressed in ganglion cell layer (GCL) and inner plexiform layer (IPL) (PubMed:23805044).2 Publications

Developmental stagei

Expressed weakly in ganglion cell layer (GCL) and inner neuroblastic layer (NBL) of the embryonic retina at 15.5 dpc. Expression increases progressively in the retina from new borns at postnatal day 2 (P2), P5, P15 to 8 week-old adult (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000057455
CleanExiMM_RIT2
ExpressionAtlasiP70425 baseline and differential
GenevisibleiP70425 MM

Interactioni

Subunit structurei

Interacts with PLXNB3 (By similarity). Interacts with AFDN, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Binds calmodulin. Interacts with POU4F1 (via N-terminus); the interaction controls POU4F1 transactivation activity on some neuronal target genes (PubMed:12934100).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RLFQ131292EBI-2649620,EBI-958266From Homo sapiens.

GO - Molecular functioni

  • calmodulin binding Source: ParkinsonsUK-UCL
  • semaphorin receptor binding Source: MGI

Protein-protein interaction databases

BioGridi202893, 1 interactor
IntActiP70425, 3 interactors
STRINGi10090.ENSMUSP00000114323

Structurei

3D structure databases

ProteinModelPortaliP70425
SMRiP70425
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133678
HOVERGENiHBG009351
InParanoidiP70425
KOiK07833
OMAiRFLDYHD
OrthoDBiEOG091G0UAU
PhylomeDBiP70425
TreeFamiTF315072

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequencei

Sequence statusi: Complete.

P70425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVENEAHCC PGSSSGGSRE YKVVMLGAGG VGKSAVTMQF ISHQFPDYHD
60 70 80 90 100
PTIEDAYKTQ VRIDNEPAYL DILDTAGQAE FTAMREQYMR GGEGFIICYS
110 120 130 140 150
VTDRQSFQEA AKFKELIFQV RHTYEIPLVL VGNKIDLEQF RQVSTEEGMN
160 170 180 190 200
LARDYNCAFF ETSAALRFGI DDAFQGLVRE IRRKESMLSL VERKLKRKDS
210
LWKKIKASLK KKRENML
Length:217
Mass (Da):24,802
Last modified:February 1, 1997 - v1
Checksum:i189AA6DCCE75034F
GO

Sequence cautioni

The sequence BAE23581 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3V → A in AAD13022 (PubMed:10545207).Curated1
Sequence conflicti150N → T in AAD13022 (PubMed:10545207).Curated1
Sequence conflicti176G → S in BAC32750 (PubMed:16141072).Curated1
Sequence conflicti217L → I in AAD13022 (PubMed:10545207).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71202 mRNA Translation: AAB42212.1
AF084463 mRNA Translation: AAD13022.1
AK040743 mRNA Translation: BAC30690.1
AK046425 mRNA Translation: BAC32724.1
AK046484 mRNA Translation: BAC32750.1
AK138206 mRNA Translation: BAE23581.1 Different initiation.
AK139394 mRNA Translation: BAE23992.1
AK140133 mRNA Translation: BAE24250.1
AK140726 mRNA Translation: BAE24457.1
AK162862 mRNA Translation: BAE37088.1
AK162953 mRNA Translation: BAE37131.1
BC018267 mRNA Translation: AAH18267.1
CCDSiCCDS29108.1
RefSeqiNP_033091.1, NM_009065.2
UniGeneiMm.5163

Genome annotation databases

EnsembliENSMUST00000153060; ENSMUSP00000114323; ENSMUSG00000057455
GeneIDi19762
KEGGimmu:19762
UCSCiuc008ehx.1 mouse

Similar proteinsi

Entry informationi

Entry nameiRIT2_MOUSE
AccessioniPrimary (citable) accession number: P70425
Secondary accession number(s): Q3UST1
, Q3UUP4, Q8BQT5, Q9QWX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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