Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P70425

- RIT2_MOUSE

UniProt

P70425 - RIT2_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

GTP-binding protein Rit2

Gene

Rit2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Binds and exchanges GTP and GDP.

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 348GTPBy similarity
Nucleotide bindingi74 – 785GTPBy similarity
Nucleotide bindingi133 – 1364GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. GTP catabolic process Source: InterPro
  2. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_252757. Signalling to p38 via RIT and RIN.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein Rit2
Alternative name(s):
Ras-like protein expressed in neurons
Ras-like without CAAX protein 2
Gene namesi
Name:Rit2
Synonyms:Rin, Roc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:108054. Rit2.

Subcellular locationi

Nucleus By similarity. Cell membrane
Note: Colocalizes with PLXNB3 at the plasma membrane.By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi34 – 341S → N: Dominant negative. Loss of interaction with MLLT4, RLF and RALGDS. 1 Publication
Mutagenesisi52 – 521T → A: Loss of interaction with MLLT4, RLF and RALGDS; when associated with L-78. 1 Publication
Mutagenesisi78 – 781Q → L: Constitutively active. Dramatic reduction of the rate of GTP hydrolysis. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217GTP-binding protein Rit2PRO_0000082728Add
BLAST

Proteomic databases

MaxQBiP70425.
PaxDbiP70425.
PRIDEiP70425.

Expressioni

Tissue specificityi

Neuron-specific.

Gene expression databases

BgeeiP70425.
CleanExiMM_RIT2.
ExpressionAtlasiP70425. baseline and differential.
GenevestigatoriP70425.

Interactioni

Subunit structurei

Interacts with PLXNB3 (By similarity). Interacts with MLLT4, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Binds calmodulin.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RLFQ131292EBI-2649620,EBI-958266From a different organism.

Protein-protein interaction databases

BioGridi202893. 1 interaction.
IntActiP70425. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliP70425.
SMRiP70425. Positions 33-183.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00770000120468.
HOVERGENiHBG009351.
InParanoidiP70425.
KOiK07833.
OMAiVYKIVIL.
PhylomeDBiP70425.
TreeFamiTF315072.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70425-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEVENEAHCC PGSSSGGSRE YKVVMLGAGG VGKSAVTMQF ISHQFPDYHD
60 70 80 90 100
PTIEDAYKTQ VRIDNEPAYL DILDTAGQAE FTAMREQYMR GGEGFIICYS
110 120 130 140 150
VTDRQSFQEA AKFKELIFQV RHTYEIPLVL VGNKIDLEQF RQVSTEEGMN
160 170 180 190 200
LARDYNCAFF ETSAALRFGI DDAFQGLVRE IRRKESMLSL VERKLKRKDS
210
LWKKIKASLK KKRENML
Length:217
Mass (Da):24,802
Last modified:February 1, 1997 - v1
Checksum:i189AA6DCCE75034F
GO

Sequence cautioni

The sequence BAE23581.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31V → A in AAD13022. (PubMed:10545207)Curated
Sequence conflicti150 – 1501N → T in AAD13022. (PubMed:10545207)Curated
Sequence conflicti176 – 1761G → S in BAC32750. (PubMed:16141072)Curated
Sequence conflicti217 – 2171L → I in AAD13022. (PubMed:10545207)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71202 mRNA. Translation: AAB42212.1.
AF084463 mRNA. Translation: AAD13022.1.
AK040743 mRNA. Translation: BAC30690.1.
AK046425 mRNA. Translation: BAC32724.1.
AK046484 mRNA. Translation: BAC32750.1.
AK138206 mRNA. Translation: BAE23581.1. Different initiation.
AK139394 mRNA. Translation: BAE23992.1.
AK140133 mRNA. Translation: BAE24250.1.
AK140726 mRNA. Translation: BAE24457.1.
AK162862 mRNA. Translation: BAE37088.1.
AK162953 mRNA. Translation: BAE37131.1.
BC018267 mRNA. Translation: AAH18267.1.
CCDSiCCDS29108.1.
RefSeqiNP_033091.1. NM_009065.2.
UniGeneiMm.5163.

Genome annotation databases

EnsembliENSMUST00000153060; ENSMUSP00000114323; ENSMUSG00000057455.
GeneIDi19762.
KEGGimmu:19762.
UCSCiuc008ehx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71202 mRNA. Translation: AAB42212.1 .
AF084463 mRNA. Translation: AAD13022.1 .
AK040743 mRNA. Translation: BAC30690.1 .
AK046425 mRNA. Translation: BAC32724.1 .
AK046484 mRNA. Translation: BAC32750.1 .
AK138206 mRNA. Translation: BAE23581.1 . Different initiation.
AK139394 mRNA. Translation: BAE23992.1 .
AK140133 mRNA. Translation: BAE24250.1 .
AK140726 mRNA. Translation: BAE24457.1 .
AK162862 mRNA. Translation: BAE37088.1 .
AK162953 mRNA. Translation: BAE37131.1 .
BC018267 mRNA. Translation: AAH18267.1 .
CCDSi CCDS29108.1.
RefSeqi NP_033091.1. NM_009065.2.
UniGenei Mm.5163.

3D structure databases

ProteinModelPortali P70425.
SMRi P70425. Positions 33-183.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202893. 1 interaction.
IntActi P70425. 3 interactions.

Proteomic databases

MaxQBi P70425.
PaxDbi P70425.
PRIDEi P70425.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000153060 ; ENSMUSP00000114323 ; ENSMUSG00000057455 .
GeneIDi 19762.
KEGGi mmu:19762.
UCSCi uc008ehx.1. mouse.

Organism-specific databases

CTDi 6014.
MGIi MGI:108054. Rit2.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00770000120468.
HOVERGENi HBG009351.
InParanoidi P70425.
KOi K07833.
OMAi VYKIVIL.
PhylomeDBi P70425.
TreeFami TF315072.

Enzyme and pathway databases

Reactomei REACT_252757. Signalling to p38 via RIT and RIN.

Miscellaneous databases

ChiTaRSi Rit2. mouse.
NextBioi 297208.
PROi P70425.
SOURCEi Search...

Gene expression databases

Bgeei P70425.
CleanExi MM_RIT2.
ExpressionAtlasi P70425. baseline and differential.
Genevestigatori P70425.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view ]
PANTHERi PTHR24070. PTHR24070. 1 hit.
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00173. RAS. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51421. RAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rin, a neuron-specific and calmodulin-binding small G-protein, and Rit define a novel subfamily of ras proteins."
    Lee C.H.J., Della N.G., Chew C.E., Zack D.J.
    J. Neurosci. 16:6784-6794(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Tissue: Retina.
  2. "Biochemical characterization of the Ras-related GTPases Rit and Rin."
    Shao H., Kadono-Okuda K., Finlin B.S., Andres D.A.
    Arch. Biochem. Biophys. 371:207-219(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, INTERACTION WITH MLLT4; RP RALGDS AND RLF, MUTAGENESIS OF SER-34; THR-52 AND GLN-78.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Blood vessel, Brain cortex, Corpora quadrigemina, Corpus striatum, Hypothalamus and Spinal cord.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Retina.

Entry informationi

Entry nameiRIT2_MOUSE
AccessioniPrimary (citable) accession number: P70425
Secondary accession number(s): Q3UST1
, Q3UUP4, Q8BQT5, Q9QWX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: February 1, 1997
Last modified: November 26, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are much faster than those of most Ras subfamily members.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3