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Protein

GTP-binding protein Rit2

Gene

Rit2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds and exchanges GTP and GDP. Binds and modulates the activation of POU4F1 as gene expression regulator.1 Publication

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 34GTPBy similarity8
Nucleotide bindingi74 – 78GTPBy similarity5
Nucleotide bindingi133 – 136GTPBy similarity4

GO - Molecular functioni

  • calmodulin binding Source: ParkinsonsUK-UCL
  • chromatin binding Source: ParkinsonsUK-UCL
  • GTPase activity Source: ParkinsonsUK-UCL
  • GTP binding Source: ParkinsonsUK-UCL
  • semaphorin receptor binding Source: MGI

GO - Biological processi

  • intracellular signal transduction Source: MGI
  • maintenance of protein location in cell Source: MGI
  • negative regulation of neuron projection development Source: ParkinsonsUK-UCL
  • positive regulation of MAPK cascade Source: ParkinsonsUK-UCL
  • positive regulation of neuron projection development Source: ParkinsonsUK-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: ParkinsonsUK-UCL
  • Ras protein signal transduction Source: ParkinsonsUK-UCL
  • regulation of Cdc42 protein signal transduction Source: ParkinsonsUK-UCL
  • regulation of endocytosis Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding, GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein Rit2
Alternative name(s):
Ras-like protein expressed in neurons
Ras-like without CAAX protein 2
Gene namesi
Name:Rit2
Synonyms:Rin, Roc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:108054. Rit2.

Subcellular locationi

  • Nucleus By similarity
  • Cell membrane By similarity

  • Note: Colocalizes with PLXNB3 at the plasma membrane.By similarity

GO - Cellular componenti

  • cell body Source: ParkinsonsUK-UCL
  • cytoplasm Source: ParkinsonsUK-UCL
  • dendritic tree Source: ParkinsonsUK-UCL
  • membrane raft Source: MGI
  • neuron projection Source: MGI
  • nucleus Source: ParkinsonsUK-UCL
  • plasma membrane Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi34S → N: Dominant negative. Loss of interaction with AFDN, RLF and RALGDS. 1 Publication1
Mutagenesisi52T → A: Loss of interaction with AFDN, RLF and RALGDS; when associated with L-78. 1 Publication1
Mutagenesisi78Q → L: Constitutively active. Dramatic reduction of the rate of GTP hydrolysis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000827281 – 217GTP-binding protein Rit2Add BLAST217

Proteomic databases

MaxQBiP70425.
PaxDbiP70425.
PRIDEiP70425.

PTM databases

PhosphoSitePlusiP70425.

Expressioni

Tissue specificityi

Neuron-specific.

Gene expression databases

BgeeiENSMUSG00000057455.
CleanExiMM_RIT2.
ExpressionAtlasiP70425. baseline and differential.
GenevisibleiP70425. MM.

Interactioni

Subunit structurei

Interacts with PLXNB3 (By similarity). Interacts with AFDN, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Binds calmodulin. Interacts with POU4F1 (via N-terminus); the interation controls POU4F1 transactivation activity on some neuronal target genes (PubMed:12934100).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RLFQ131292EBI-2649620,EBI-958266From a different organism.

GO - Molecular functioni

  • calmodulin binding Source: ParkinsonsUK-UCL
  • semaphorin receptor binding Source: MGI

Protein-protein interaction databases

BioGridi202893. 1 interactor.
IntActiP70425. 3 interactors.
STRINGi10090.ENSMUSP00000114323.

Structurei

3D structure databases

ProteinModelPortaliP70425.
SMRiP70425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133678.
HOVERGENiHBG009351.
InParanoidiP70425.
KOiK07833.
OMAiRCGEGFM.
OrthoDBiEOG091G0UAU.
PhylomeDBiP70425.
TreeFamiTF315072.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVENEAHCC PGSSSGGSRE YKVVMLGAGG VGKSAVTMQF ISHQFPDYHD
60 70 80 90 100
PTIEDAYKTQ VRIDNEPAYL DILDTAGQAE FTAMREQYMR GGEGFIICYS
110 120 130 140 150
VTDRQSFQEA AKFKELIFQV RHTYEIPLVL VGNKIDLEQF RQVSTEEGMN
160 170 180 190 200
LARDYNCAFF ETSAALRFGI DDAFQGLVRE IRRKESMLSL VERKLKRKDS
210
LWKKIKASLK KKRENML
Length:217
Mass (Da):24,802
Last modified:February 1, 1997 - v1
Checksum:i189AA6DCCE75034F
GO

Sequence cautioni

The sequence BAE23581 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3V → A in AAD13022 (PubMed:10545207).Curated1
Sequence conflicti150N → T in AAD13022 (PubMed:10545207).Curated1
Sequence conflicti176G → S in BAC32750 (PubMed:16141072).Curated1
Sequence conflicti217L → I in AAD13022 (PubMed:10545207).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71202 mRNA. Translation: AAB42212.1.
AF084463 mRNA. Translation: AAD13022.1.
AK040743 mRNA. Translation: BAC30690.1.
AK046425 mRNA. Translation: BAC32724.1.
AK046484 mRNA. Translation: BAC32750.1.
AK138206 mRNA. Translation: BAE23581.1. Different initiation.
AK139394 mRNA. Translation: BAE23992.1.
AK140133 mRNA. Translation: BAE24250.1.
AK140726 mRNA. Translation: BAE24457.1.
AK162862 mRNA. Translation: BAE37088.1.
AK162953 mRNA. Translation: BAE37131.1.
BC018267 mRNA. Translation: AAH18267.1.
CCDSiCCDS29108.1.
RefSeqiNP_033091.1. NM_009065.2.
UniGeneiMm.5163.

Genome annotation databases

EnsembliENSMUST00000153060; ENSMUSP00000114323; ENSMUSG00000057455.
GeneIDi19762.
KEGGimmu:19762.
UCSCiuc008ehx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71202 mRNA. Translation: AAB42212.1.
AF084463 mRNA. Translation: AAD13022.1.
AK040743 mRNA. Translation: BAC30690.1.
AK046425 mRNA. Translation: BAC32724.1.
AK046484 mRNA. Translation: BAC32750.1.
AK138206 mRNA. Translation: BAE23581.1. Different initiation.
AK139394 mRNA. Translation: BAE23992.1.
AK140133 mRNA. Translation: BAE24250.1.
AK140726 mRNA. Translation: BAE24457.1.
AK162862 mRNA. Translation: BAE37088.1.
AK162953 mRNA. Translation: BAE37131.1.
BC018267 mRNA. Translation: AAH18267.1.
CCDSiCCDS29108.1.
RefSeqiNP_033091.1. NM_009065.2.
UniGeneiMm.5163.

3D structure databases

ProteinModelPortaliP70425.
SMRiP70425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202893. 1 interactor.
IntActiP70425. 3 interactors.
STRINGi10090.ENSMUSP00000114323.

PTM databases

PhosphoSitePlusiP70425.

Proteomic databases

MaxQBiP70425.
PaxDbiP70425.
PRIDEiP70425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000153060; ENSMUSP00000114323; ENSMUSG00000057455.
GeneIDi19762.
KEGGimmu:19762.
UCSCiuc008ehx.1. mouse.

Organism-specific databases

CTDi6014.
MGIiMGI:108054. Rit2.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133678.
HOVERGENiHBG009351.
InParanoidiP70425.
KOiK07833.
OMAiRCGEGFM.
OrthoDBiEOG091G0UAU.
PhylomeDBiP70425.
TreeFamiTF315072.

Miscellaneous databases

ChiTaRSiRit2. mouse.
PROiP70425.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057455.
CleanExiMM_RIT2.
ExpressionAtlasiP70425. baseline and differential.
GenevisibleiP70425. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIT2_MOUSE
AccessioniPrimary (citable) accession number: P70425
Secondary accession number(s): Q3UST1
, Q3UUP4, Q8BQT5, Q9QWX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are much faster than those of most Ras subfamily members.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.