Reviewed,
UniProtKB/Swiss-Prot P70419 (GALT3_MOUSE)
Last modified
October 13, 2009.
Version 75.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Polypeptide N-acetylgalactosaminyltransferase 3 EC=2.4.1.41 Alternative name(s): Polypeptide GalNAc transferase 3 Short name=pp-GaNTase 3 Short name=GalNAc-T3 Protein-UDP acetylgalactosaminyltransferase 3 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 633 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward HIV envelope glycoprotein gp120, EA2, Muc2 and Muc5. Glycosylates FGF23 By similarity. Probably glycosylates fibronectin in vivo. May be involved in phosphate homeostasis. |
| Catalytic activity | UDP-N-acetyl-D-galactosamine + polypeptide = UDP + N-acetyl-D-galactosaminyl-polypeptide. Ref.1 |
| Cofactor | Manganese By similarity. Calcium By similarity. |
| Pathway | |
| Subcellular location | Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Resides preferentially in the trans and medial parts of the Golgi stack By similarity. |
| Tissue specificity | Highly expressed in the reproductive tract, principally in the testis and uterus, and to a lesser degree in the cervix with only trace levels in the ovary. Also expressed at high level in sublingual gland, stomach and colon, with more moderate amounts present in the submandibular and parotid gland as well as the kidney. Ref.1 |
| Domain | There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity. The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity. |
| Sequence similarities | Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Golgi apparatus Membrane |
| Domain | Signal-anchor Transmembrane |
| Ligand | Calcium Lectin Manganese |
| Molecular function | Glycosyltransferase Transferase |
| PTM | Disulfide bond Glycoprotein |
| Gene Ontology (GO) | |
| Cellular component | Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW polypeptide N-acetylgalactosaminyltransferase activityInferred from electronic annotation. Source: EC sugar bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 633 | 633 | Polypeptide N-acetylgalactosaminyltransferase 3 | PRO_0000059107 | |||||||
Regions | |||||||||||
| Topological domain | 1 – 19 | 19 | Cytoplasmic Potential | ||||||||
| Transmembrane | 20 – 37 | 18 | Signal-anchor for type II membrane protein Potential | ||||||||
| Topological domain | 38 – 633 | 596 | Lumenal Potential | ||||||||
| Domain | 504 – 630 | 127 | Ricin B-type lectin | ||||||||
| Region | 184 – 293 | 110 | Catalytic subdomain A | ||||||||
| Region | 356 – 418 | 63 | Catalytic subdomain B | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 297 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 484 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 517 ↔ 535 | By similarity | |||||||||
| Disulfide bond | 561 ↔ 574 | By similarity | |||||||||
| Disulfide bond | 605 ↔ 618 | By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 324 | 1 | N → S in AAB09579. Ref.1 | ||||||||
| Sequence conflict | 633 | 1 | D → E in AAB09579. Ref.1 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and expression of mouse UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T3." Zara J., Hagen F.K., Ten Hagen K.G., Van Wuyckhuyse B.C., Tabak L.A. Biochem. Biophys. Res. Commun. 228:38-44(1996) [PubMed: 8912633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY. Strain: CD-1. Tissue: Testis. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor. |
| + | Additional computationally mapped references. |
Web resources
| Functional Glycomics Gateway - GTase Polypeptide N-acetylgalactosaminyltransferase 3 |
Cross-references
Sequence databases | |
|---|---|
| U70538 mRNA. Translation: AAB09579.1. BC043331 mRNA. Translation: AAH43331.1. | |
| IPI | IPI00330383. |
| PIR | JC5247. |
| UniGene | Mm.439760 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P70419. |
Protein family/group databases | |
| CAZy | CBM13. Carbohydrate-Binding Module Family 13. GT27. Glycosyltransferase Family 27. |
Proteomic databases | |
| PRIDE | P70419. |
Genome annotation databases | |
| Ensembl | ENSMUST00000028378; ENSMUSP00000028378; ENSMUSG00000026994; Mus musculus. [Genome view] |
Organism-specific databases | |
| MGI | MGI:894695. Galnt3. |
Phylogenomic databases | |
| HOGENOM | P70419. |
| HOVERGEN | P70419. |
Enzyme and pathway databases | |
| BRENDA | 2.4.1.41. 244. |
Gene expression databases | |
| ArrayExpress | P70419. |
| Bgee | P70419. |
| Genevestigator | P70419. |
| GermOnline | ENSMUSG00000026994. Mus musculus. |
Family and domain databases | |
| InterPro | IPR001173. Glyco_trans_2. IPR000772. Ricin_B_lectin. [Graphical view] |
| Pfam | PF00535. Glycos_transf_2. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view] |
| SMART | SM00458. RICIN. 1 hit. [Graphical view] |
| PROSITE | PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | GALT3_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P70419 Secondary accession number(s): Q80V55 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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