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P70419

- GALT3_MOUSE

UniProt

P70419 - GALT3_MOUSE

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Protein
Polypeptide N-acetylgalactosaminyltransferase 3
Gene
Galnt3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward HIV envelope glycoprotein gp120, EA2, Muc2 and Muc5. Glycosylates FGF23 By similarity. Probably glycosylates fibronectin in vivo. May be involved in phosphate homeostasis.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

Cofactori

Manganese By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei225 – 2251Substrate By similarity
Binding sitei254 – 2541Substrate By similarity
Metal bindingi277 – 2771Manganese By similarity
Metal bindingi279 – 2791Manganese By similarity
Binding sitei387 – 3871Substrate By similarity
Metal bindingi415 – 4151Manganese By similarity
Binding sitei418 – 4181Substrate By similarity

GO - Molecular functioni

  1. calcium ion binding Source: Ensembl
  2. manganese ion binding Source: Ensembl
  3. polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein O-linked glycosylation via serine Source: Ensembl
  2. protein O-linked glycosylation via threonine Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.41. 3474.
ReactomeiREACT_198517. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 3 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 3
Short name:
GalNAc-T3
Short name:
pp-GaNTase 3
Protein-UDP acetylgalactosaminyltransferase 3
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3
Gene namesi
Name:Galnt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:894695. Galnt3.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity
Note: Resides preferentially in the trans and medial parts of the Golgi stack By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei20 – 3718Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini38 – 633596Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. nucleus Source: Ensembl
  4. perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 633633Polypeptide N-acetylgalactosaminyltransferase 3
PRO_0000059107Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi173 ↔ 410 By similarity
Glycosylationi297 – 2971N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi401 ↔ 482 By similarity
Glycosylationi484 – 4841N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi517 ↔ 535 By similarity
Disulfide bondi561 ↔ 574 By similarity
Disulfide bondi605 ↔ 618 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP70419.
PaxDbiP70419.
PRIDEiP70419.

PTM databases

PhosphoSiteiP70419.

Expressioni

Tissue specificityi

Highly expressed in the reproductive tract, principally in the testis and uterus, and to a lesser degree in the cervix with only trace levels in the ovary. Also expressed at high level in sublingual gland, stomach and colon, with more moderate amounts present in the submandibular and parotid gland as well as the kidney.1 Publication

Gene expression databases

BgeeiP70419.
GenevestigatoriP70419.

Interactioni

Protein-protein interaction databases

IntActiP70419. 1 interaction.
MINTiMINT-4095534.
STRINGi10090.ENSMUSP00000028378.

Structurei

3D structure databases

ProteinModelPortaliP70419.
SMRiP70419. Positions 122-633.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini504 – 630127Ricin B-type lectin
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni184 – 293110Catalytic subdomain A
Add
BLAST
Regioni356 – 41863Catalytic subdomain B
Add
BLAST

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG239675.
GeneTreeiENSGT00750000117451.
HOGENOMiHOG000038227.
HOVERGENiHBG051699.
InParanoidiQ3UXL2.
KOiK00710.
OMAiQYFEYSA.
OrthoDBiEOG7J9VP2.
TreeFamiTF313267.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70419-1 [UniParc]FASTAAdd to Basket

« Hide

MAHLKRLVKL HIKRHYHRKF WKLGAVIFFF LVVLILMQRE VSVQYSKEES    50
KMERNLKNKN KMLDFMLEAV NNIKDAMPKM QIGAPIKENI DVRERPCLQG 100
YYTAAELKPV FDRPPQDSNA PGASGKPFKI THLSPEEQKE KERGETKHCF 150
NAFASDRISL HRDLGPDTRP PECIEQKFKR CPPLPTTSVI IVFHNEAWST 200
LLRTVHSVLY SSPAILLKEI ILVDDASVDD YLHEKLEEYI KQFSIVKIVR 250
QQERKGLITA RLLGAAVATA ETLTFLDAHC ECFYGWLEPL LARIAENYTA 300
VVSPDIASID LNTFEFNKPS PYGSNHNRGN FDWSLSFGWE SLPDHEKQRR 350
KDETYPIKTP TFAGGLFSIS KKYFEHIGSY DEEMEIWGGE NIEMSFRVWQ 400
CGGQLEIMPC SVVGHVFRSK SPHTFPKGTQ VIARNQVRLA EVWMDEYKEI 450
FYRRNTDAAK IVKQKSFGDL SKRFEIKKRL QCKNFTWYLN TIYPEAYVPD 500
LNPVISGYIK SVGQPLCLDV GENNQGGKPL ILYTCHGLGG NQYFEYSAQR 550
EIRHNIQKEL CLHATQGVVQ LKACVYKGHR TIAPGEQIWE IRKDQLLYNP 600
LFKMCLSSNG EHPNLVPCDA TDLLQKWIFS QND 633
Length:633
Mass (Da):72,932
Last modified:July 27, 2011 - v3
Checksum:i8BEE7CDDBCF56428
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti324 – 3241S → N in AAH43331. 1 Publication
Sequence conflicti633 – 6331D → E in AAB09579. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U70538 mRNA. Translation: AAB09579.1.
AK135489 mRNA. Translation: BAE22551.1.
AL929230, AL928586 Genomic DNA. Translation: CAM20333.1.
AL928586, AL929230 Genomic DNA. Translation: CAM21878.1.
CH466519 Genomic DNA. Translation: EDL27008.1.
BC043331 mRNA. Translation: AAH43331.1.
CCDSiCCDS16075.1.
PIRiJC5247.
RefSeqiNP_056551.2. NM_015736.2.
UniGeneiMm.439760.

Genome annotation databases

EnsembliENSMUST00000028378; ENSMUSP00000028378; ENSMUSG00000026994.
GeneIDi14425.
KEGGimmu:14425.
UCSCiuc008jwu.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Polypeptide N-acetylgalactosaminyltransferase 3

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U70538 mRNA. Translation: AAB09579.1 .
AK135489 mRNA. Translation: BAE22551.1 .
AL929230 , AL928586 Genomic DNA. Translation: CAM20333.1 .
AL928586 , AL929230 Genomic DNA. Translation: CAM21878.1 .
CH466519 Genomic DNA. Translation: EDL27008.1 .
BC043331 mRNA. Translation: AAH43331.1 .
CCDSi CCDS16075.1.
PIRi JC5247.
RefSeqi NP_056551.2. NM_015736.2.
UniGenei Mm.439760.

3D structure databases

ProteinModelPortali P70419.
SMRi P70419. Positions 122-633.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P70419. 1 interaction.
MINTi MINT-4095534.
STRINGi 10090.ENSMUSP00000028378.

Protein family/group databases

CAZyi CBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

PTM databases

PhosphoSitei P70419.

Proteomic databases

MaxQBi P70419.
PaxDbi P70419.
PRIDEi P70419.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028378 ; ENSMUSP00000028378 ; ENSMUSG00000026994 .
GeneIDi 14425.
KEGGi mmu:14425.
UCSCi uc008jwu.2. mouse.

Organism-specific databases

CTDi 2591.
MGIi MGI:894695. Galnt3.

Phylogenomic databases

eggNOGi NOG239675.
GeneTreei ENSGT00750000117451.
HOGENOMi HOG000038227.
HOVERGENi HBG051699.
InParanoidi Q3UXL2.
KOi K00710.
OMAi QYFEYSA.
OrthoDBi EOG7J9VP2.
TreeFami TF313267.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BRENDAi 2.4.1.41. 3474.
Reactomei REACT_198517. O-linked glycosylation of mucins.

Miscellaneous databases

ChiTaRSi GALNT3. mouse.
NextBioi 286025.
PROi P70419.
SOURCEi Search...

Gene expression databases

Bgeei P70419.
Genevestigatori P70419.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view ]
Pfami PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view ]
SMARTi SM00458. RICIN. 1 hit.
[Graphical view ]
SUPFAMi SSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of mouse UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T3."
    Zara J., Hagen F.K., Ten Hagen K.G., Van Wuyckhuyse B.C., Tabak L.A.
    Biochem. Biophys. Res. Commun. 228:38-44(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY.
    Strain: CD-1.
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Muellerian duct.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiGALT3_MOUSE
AccessioniPrimary (citable) accession number: P70419
Secondary accession number(s): Q3UXL2, Q80V55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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