Sodium/calcium exchanger 1 precursor

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P70414 (NAC1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sodium/calcium exchanger 1

Alternative name(s):
Na(+)/Ca(2+)-exchange protein 1

Gene names

Name:	Slc8a1
Synonyms:	Ncx

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	970 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Rapidly transports Ca²⁺ during excitation-contraction coupling. Ca²⁺ is extruded from the cell during relaxation so as to prevent overloading of intracellular stores.
Enzyme regulation	By ATP.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Cardiac sarcolemma.
Sequence similarities	Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily. Contains 2 Calx-beta domains.

Ontologies

Keywords
Biological process	Antiport Calcium transport Ion transport Sodium transport Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium Calmodulin-binding Metal-binding Sodium
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	calcium ion import Inferred from mutant phenotype PubMed 10967099. Source: BHF-UCL calcium ion transport into cytosol Inferred from mutant phenotype PubMed 10967099. Source: BHF-UCL cardiac muscle cell development Inferred from mutant phenotype PubMed 10967099. Source: BHF-UCL cardiac muscle contraction Inferred from genetic interaction PubMed 15308581. Source: MGI cell communication Inferred from electronic annotation. Source: InterPro cellular response to caffeine Inferred from mutant phenotype PubMed 10967099. Source: BHF-UCL embryonic heart tube development Inferred from mutant phenotype PubMed 21302256. Source: MGI embryonic placenta development Inferred from mutant phenotype PubMed 21302256. Source: MGI heart morphogenesis Inferred from genetic interaction PubMed 15308581. Source: MGI muscle fiber development Inferred from genetic interaction PubMed 15308581. Source: MGI post-embryonic development Inferred from genetic interaction PubMed 15308581. Source: MGI reduction of cytosolic calcium ion concentration Inferred from mutant phenotype PubMed 10967099. Source: BHF-UCL regulation of heart rate Inferred from mutant phenotype PubMed 10967099. Source: BHF-UCL regulation of the force of heart contraction Inferred from mutant phenotype PubMed 12767889. Source: MGI relaxation of smooth muscle Inferred from mutant phenotype PubMed 10967099. Source: BHF-UCL sodium ion export Inferred from mutant phenotype PubMed 10967099. Source: BHF-UCL vascular smooth muscle contraction Inferred from mutant phenotype PubMed 10967099. Source: BHF-UCL
Cellular_component	T-tubule Inferred from direct assay PubMed 16292983. Source: BHF-UCL basolateral plasma membrane Inferred from direct assay PubMed 11423561. Source: MGI integral to membrane Traceable author statement PubMed 15336980. Source: MGI
Molecular_function	calcium:sodium antiporter activity Inferred from mutant phenotype PubMed 10967099. Source: BHF-UCL metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Nanog	Q80Z64	9	EBI-2312694,EBI-2312517
Nr0b1	Q61066	2	EBI-2312694,EBI-2312665

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 32

Potential

Chain

33 – 970

938

Sodium/calcium exchanger 1

PRO_0000019380

Regions

Topological domain

33 – 71

Extracellular Potential

Transmembrane

72 – 93

Helical; Potential

Topological domain

94 – 133

Cytoplasmic Potential

Transmembrane

134 – 155

Helical; Potential

Topological domain

156 – 167

Extracellular Potential

Transmembrane

168 – 188

Helical; Potential

Topological domain

189 – 199

Cytoplasmic Potential

Transmembrane

200 – 222

Helical; Potential

Topological domain

223 – 225

Extracellular Potential

Transmembrane

226 – 249

Helical; Potential

Topological domain

250 – 769

520

Cytoplasmic Potential

Transmembrane

770 – 789

Helical; Potential

Topological domain

790 – 796

Extracellular Potential

Transmembrane

797 – 819

Helical; Potential

Topological domain

820 – 821

Cytoplasmic Potential

Transmembrane

822 – 840

Helical; Potential

Topological domain

841 – 871

Extracellular Potential

Transmembrane

872 – 892

Helical; Potential

Topological domain

893 – 903

Cytoplasmic Potential

Transmembrane

904 – 924

Helical; Potential

Topological domain

925 – 941

Extracellular Potential

Transmembrane

942 – 958

Helical; Potential

Topological domain

959 – 970

Cytoplasmic Potential

Repeat

138 – 178

Alpha-1

Domain

393 – 493

101

Calx-beta 1

Domain

524 – 624

101

Calx-beta 2

Repeat

839 – 875

Alpha-2

Region

251 – 270

Calmodulin-binding Potential

Compositional bias

236 – 239

Poly-Phe

Compositional bias

689 – 692

Poly-Glu

Compositional bias

756 – 760

Poly-Asp

Sites

Metal binding

417

Calcium 3 By similarity

Metal binding

453

Calcium 1 By similarity

Metal binding

453

Calcium 2 By similarity

Metal binding

478

Calcium 4 By similarity

Metal binding

479

Calcium 3 By similarity

Metal binding

479

Calcium 4; via carbonyl oxygen By similarity

Metal binding

481

Calcium 3; via carbonyl oxygen By similarity

Metal binding

483

Calcium 1 By similarity

Metal binding

483

Calcium 2 By similarity

Metal binding

483

Calcium 3 By similarity

Metal binding

486

Calcium 1 By similarity

Metal binding

530

Calcium 3 By similarity

Metal binding

531

Calcium 4 By similarity

Metal binding

532

Calcium 3 By similarity

Metal binding

532

Calcium 4 By similarity

Metal binding

548

Calcium 6 By similarity

Metal binding

584

Calcium 5 By similarity

Metal binding

610

Calcium 5 By similarity

Metal binding

610

Calcium 6 By similarity

Metal binding

612

Calcium 6 By similarity

Metal binding

680

Calcium 6 By similarity

Amino acid modifications

Modified residue

389

Phosphoserine Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

157

N-linked (GlcNAc...) Potential

Glycosylation

866

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P70414 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: F5FC0BD07F2B6602
Show »

FASTA

970

108,035

        10         20         30         40         50         60 
MLRLSLPPNV SMGFRLVALV ALLFSHVDHI TADTEAETGG NETTECTGSY YCKKGVILPI 

        70         80         90        100        110        120 
WEPQDPSFGD KIARATVYFV AMVYMFLGVS IIADRFMSSI EVITSQEKEI TIKKPNGETT 

       130        140        150        160        170        180 
KTTVRIWNET VSNLTLMALG SSAPEILLSV IEVCGHNFTA GDLGPSTIVG SAAFNMFIII 

       190        200        210        220        230        240 
ALCVYVVPDG ETRKIKHLRV FFVTAAWSIF AYTWLYIILS VSSPGVVEVW EGLLTFFFFP 

       250        260        270        280        290        300 
ICVVFAWVAD RRLLFYKYVY KRYRAGKQRG MIIEHEGDRP ASKTEIEMDG KVVNSHVDNF 

       310        320        330        340        350        360 
LDGALVLEVD ERDQDDEEAR REMARILKEL KQKHPEKEIE QLIELANYQV LSQQQKSRAF 

       370        380        390        400        410        420 
YRIQATRLMT GAGNILKRHA ADQARKAVSM HEVNMEMAEN DPVSKIFFEQ GTYQCLENCG 

       430        440        450        460        470        480 
TVALTIMRRG GDLSTTVFVD FRTEDGTANA ASDYEFTEGT VIFKPGETQK EIRVGIIDDD 

       490        500        510        520        530        540 
IFEEDENFLV HLSNVRVSSD VSEDGILESN HASSIACLGS PSTATITIFD DDHAGIFTFE 

       550        560        570        580        590        600 
EPVTHVSESI GIMEVKVLRT SGARGNVIIP YKTIEGTARG GGEDFEDTCG EPEFQNDEIV 

       610        620        630        640        650        660 
KTISVKVIDD EEYEKNKTFF IEIGEPRLVE MSEKKALLLN ELGGFTLTGK EMYGQPIFRK 

       670        680        690        700        710        720 
VHARDHPIPS TVITISEEYD DKQPLTSKEE EERRIAEMGR PILGEHTKLE VIIQESYEFK 

       730        740        750        760        770        780 
STVDKLIKKT NLALVVGTNS WREQFIEAIT VSAGEDDDDD ECGEEKLPSC FDYVMHFLTV 

       790        800        810        820        830        840 
FWKVLFAFVP PTEYWNGWAC FIVSILMIGL LTAFIGDLAS HFGCTIGLKD SVTAVVFVAL 

       850        860        870        880        890        900 
GTSVPDTFAS KVAATQDQYA DASIGNVTGS NAVNVFLGIG VAWSIAAIYH AANGEQFKVS 

       910        920        930        940        950        960 
PGTLAFSVTL FTIFAFINVG VLLYRRRPEI GGELGGPRTA KLLTSSLFVL LWLLYIFFSS 

       970 
LEAYCHIKGF

« Hide

References

[1]	"Cloning of the mouse cardiac Na(+)-Ca2+ exchanger and functional expression in Xenopus oocytes." Kim I., Lee C.O. Ann. N. Y. Acad. Sci. 779:126-128(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U70033 mRNA. Translation: AAB46708.1.
IPI	IPI00109213.
RefSeq	NP_001106269.1. NM_001112798.1. NP_035536.2. NM_011406.2.
UniGene	Mm.265834. Mm.489060.
3D structure databases
ProteinModelPortal	P70414.
SMR	P70414. Positions 403-724.
ModBase	Search...
Protein-protein interaction databases
IntAct	P70414. 6 interactions.
PTM databases
PhosphoSite	P70414.
Proteomic databases
PaxDb	P70414.
PRIDE	P70414.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	20541.
KEGG	mmu:20541.
UCSC	uc008drs.1. mouse.
Organism-specific databases
CTD	6546.
MGI	MGI:107956. Slc8a1.
Phylogenomic databases
eggNOG	NOG241889.
HOGENOM	HOG000266971.
HOVERGEN	HBG006441.
InParanoid	P70414.
KO	K05849.
OrthoDB	EOG495ZR4.
Gene expression databases
Genevestigator	P70414.
GermOnline	ENSMUSG00000054640. Mus musculus.
Family and domain databases
InterPro	IPR003644. Calx_beta. IPR001623. DnaJ_domain. IPR004836. Na_Ca_Ex. IPR002987. NaCa_exhngr1. IPR004837. NaCa_Exmemb. [Graphical view]
Pfam	PF03160. Calx-beta. 2 hits. PF01699. Na_Ca_ex. 2 hits. [Graphical view]
PRINTS	PR01259. NACAEXCHNGR. PR01260. NACAEXCHNGR1.
SMART	SM00237. Calx_beta. 2 hits. [Graphical view]
TIGRFAMs	TIGR00845. caca. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	SLC8A1. mouse.
NextBio	298827.
SOURCE	Search...

Entry information

Entry name

NAC1_MOUSE

Accession

Primary (citable) accession number: P70414

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents