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Protein

Mitochondrial uncoupling protein 2

Gene

Ucp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat (By similarity).By similarity

GO - Biological processi

  1. aging Source: Ensembl
  2. cellular response to amino acid starvation Source: Ensembl
  3. cellular response to glucose stimulus Source: Ensembl
  4. cellular response to insulin stimulus Source: Ensembl
  5. female pregnancy Source: Ensembl
  6. liver regeneration Source: Ensembl
  7. mitochondrial transport Source: Ensembl
  8. negative regulation of apoptotic process Source: Ensembl
  9. negative regulation of insulin secretion involved in cellular response to glucose stimulus Source: Ensembl
  10. positive regulation of cell death Source: Ensembl
  11. regulation of mitochondrial membrane potential Source: ParkinsonsUK-UCL
  12. response to fatty acid Source: Ensembl
  13. response to hypoxia Source: Ensembl
  14. response to superoxide Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_312006. The fatty acid cycling model.
REACT_336520. The proton buffering model.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial uncoupling protein 2
Short name:
UCP 2
Alternative name(s):
Solute carrier family 25 member 8
UCPH
Gene namesi
Name:Ucp2
Synonyms:Slc25a8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:109354. Ucp2.

Subcellular locationi

Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010Mitochondrial matrixSequence Analysis
Transmembranei11 – 3222Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini33 – 7745Mitochondrial intermembraneSequence AnalysisAdd
BLAST
Transmembranei78 – 10023Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini101 – 11919Mitochondrial matrixSequence AnalysisAdd
BLAST
Transmembranei120 – 13617Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini137 – 18044Mitochondrial intermembraneSequence AnalysisAdd
BLAST
Transmembranei181 – 19717Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini198 – 21417Mitochondrial matrixSequence AnalysisAdd
BLAST
Transmembranei215 – 23420Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini235 – 26834Mitochondrial intermembraneSequence AnalysisAdd
BLAST
Transmembranei269 – 29123Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini292 – 30918Mitochondrial matrixSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: UniProtKB-SubCell
  3. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Mitochondrial uncoupling protein 2PRO_0000090665Add
BLAST

Proteomic databases

PaxDbiP70406.
PRIDEiP70406.

PTM databases

PhosphoSiteiP70406.

Expressioni

Tissue specificityi

Highest in white adipose tissue, also detected in brown adipose tissue, heart and kidney. 4-6 times higher levels are detected in ob/ob and db/db mice.

Gene expression databases

BgeeiP70406.
ExpressionAtlasiP70406. baseline and differential.
GenevestigatoriP70406.

Interactioni

Subunit structurei

Acts as a dimer forming a proton channel.By similarity

Structurei

Secondary structure

1
309
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 2914Combined sources
Helixi32 – 409Combined sources
Helixi50 – 534Combined sources
Helixi62 – 7312Combined sources
Helixi75 – 795Combined sources
Helixi82 – 9514Combined sources
Turni96 – 983Combined sources
Helixi99 – 1079Combined sources
Helixi116 – 13318Combined sources
Helixi136 – 14611Combined sources
Beta strandi153 – 1553Combined sources
Helixi159 – 17012Combined sources
Helixi172 – 20231Combined sources
Turni203 – 2086Combined sources
Helixi214 – 24229Combined sources
Beta strandi247 – 2493Combined sources
Helixi253 – 26210Combined sources
Helixi267 – 2704Combined sources
Helixi274 – 29522Combined sources
Helixi301 – 3066Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LCKNMR-A14-309[»]
ProteinModelPortaliP70406.
SMRiP70406. Positions 14-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati11 – 10696Solcar 1Add
BLAST
Repeati114 – 20390Solcar 2Add
BLAST
Repeati212 – 29786Solcar 3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni276 – 29823Purine nucleotide bindingBy similarityAdd
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300436.
HOGENOMiHOG000165140.
HOVERGENiHBG009528.
InParanoidiP70406.
KOiK15103.
OMAiLLKAHLM.
OrthoDBiEOG793B7Z.
PhylomeDBiP70406.
TreeFamiTF323211.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00784. MTUNCOUPLING.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGFKATDVP PTATVKFLGA GTAACIADLI TFPLDTAKVR LQIQGESQGL
60 70 80 90 100
VRTAASAQYR GVLGTILTMV RTEGPRSLYN GLVAGLQRQM SFASVRIGLY
110 120 130 140 150
DSVKQFYTKG SEHAGIGSRL LAGSTTGALA VAVAQPTDVV KVRFQAQARA
160 170 180 190 200
GGGRRYQSTV EAYKTIAREE GIRGLWKGTS PNVARNAIVN CAELVTYDLI
210 220 230 240 250
KDTLLKANLM TDDLPCHFTS AFGAGFCTTV IASPVDVVKT RYMNSALGQY
260 270 280 290 300
HSAGHCALTM LRKEGPRAFY KGFMPSFLRL GSWNVVMFVT YEQLKRALMA

AYQSREAPF
Length:309
Mass (Da):33,374
Last modified:January 31, 1997 - v1
Checksum:i329794EEA99810E5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti285 – 2851V → I in BAA32532 (PubMed:9710252).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69135 mRNA. Translation: AAB17666.1.
U94593 mRNA. Translation: AAB53092.1.
AB012159 Genomic DNA. Translation: BAA32532.1.
BC012697 mRNA. Translation: AAH12697.1.
BC012967 mRNA. Translation: AAH12967.1.
CCDSiCCDS21498.1.
RefSeqiNP_035801.3. NM_011671.5.
XP_006507642.1. XM_006507579.1.
UniGeneiMm.171378.

Genome annotation databases

EnsembliENSMUST00000126534; ENSMUSP00000120967; ENSMUSG00000033685.
GeneIDi22228.
KEGGimmu:22228.
UCSCiuc009inb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69135 mRNA. Translation: AAB17666.1.
U94593 mRNA. Translation: AAB53092.1.
AB012159 Genomic DNA. Translation: BAA32532.1.
BC012697 mRNA. Translation: AAH12697.1.
BC012967 mRNA. Translation: AAH12967.1.
CCDSiCCDS21498.1.
RefSeqiNP_035801.3. NM_011671.5.
XP_006507642.1. XM_006507579.1.
UniGeneiMm.171378.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LCKNMR-A14-309[»]
ProteinModelPortaliP70406.
SMRiP70406. Positions 14-308.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP70406.

Proteomic databases

PaxDbiP70406.
PRIDEiP70406.

Protocols and materials databases

DNASUi22228.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000126534; ENSMUSP00000120967; ENSMUSG00000033685.
GeneIDi22228.
KEGGimmu:22228.
UCSCiuc009inb.2. mouse.

Organism-specific databases

CTDi7351.
MGIiMGI:109354. Ucp2.

Phylogenomic databases

eggNOGiNOG300436.
HOGENOMiHOG000165140.
HOVERGENiHBG009528.
InParanoidiP70406.
KOiK15103.
OMAiLLKAHLM.
OrthoDBiEOG793B7Z.
PhylomeDBiP70406.
TreeFamiTF323211.

Enzyme and pathway databases

ReactomeiREACT_312006. The fatty acid cycling model.
REACT_336520. The proton buffering model.

Miscellaneous databases

ChiTaRSiUcp2. mouse.
NextBioi302263.
PROiP70406.
SOURCEiSearch...

Gene expression databases

BgeeiP70406.
ExpressionAtlasiP70406. baseline and differential.
GenevestigatoriP70406.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00784. MTUNCOUPLING.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Raimbault S., Bouillaud F., Ricquier D.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BALB/c.
    Tissue: Muscle.
  2. "Cloning and characterization of an uncoupling protein homolog: a potential molecular mediator of human thermogenesis."
    Gimeno R.E., Dembski M., Weng X., Deng N., Shyjan A.W., Gimeno C.J., Iris F., Ellis S.J., Woolf E.A., Tartaglia L.A.
    Diabetes 46:900-906(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Spleen.
  3. "Genomic organization and promoter function of the mouse uncoupling protein 2 (UCP2) gene."
    Yamada M., Hashida T., Shibusawa N., Iwasaki T., Murakami M., Monden T., Satoh T., Mori M.
    FEBS Lett. 432:65-69(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6 X CBA.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Colon and Mammary gland.
  5. "Mitochondrial uncoupling protein 2 structure determined by NMR molecular fragment searching."
    Berardi M.J., Shih W.M., Harrison S.C., Chou J.J.
    Nature 476:109-113(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 14-309, SUBCELLULAR LOCATION, TOPOLOGY.

Entry informationi

Entry nameiUCP2_MOUSE
AccessioniPrimary (citable) accession number: P70406
Secondary accession number(s): O88285
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: January 31, 1997
Last modified: March 31, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.