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P70406 (UCP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial uncoupling protein 2

Short name=UCP 2
Alternative name(s):
Solute carrier family 25 member 8
UCPH
Gene names
Name:Ucp2
Synonyms:Slc25a8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat By similarity.

Subunit structure

Acts as a dimer forming a proton channel By similarity.

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein Ref.5.

Tissue specificity

Highest in white adipose tissue, also detected in brown adipose tissue, heart and kidney. 4-6 times higher levels are detected in ob/ob and db/db mice.

Sequence similarities

Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]

Contains 3 Solcar repeats.

Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
Mitochondrion
Mitochondrion inner membrane
   DomainRepeat
Transmembrane
Transmembrane helix
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from electronic annotation. Source: Ensembl

cellular response to amino acid starvation

Inferred from electronic annotation. Source: Ensembl

cellular response to glucose stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to insulin stimulus

Inferred from electronic annotation. Source: Ensembl

female pregnancy

Inferred from electronic annotation. Source: Ensembl

liver regeneration

Inferred from electronic annotation. Source: Ensembl

mitochondrial transport

Inferred from electronic annotation. Source: Ensembl

negative regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of insulin secretion involved in cellular response to glucose stimulus

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell death

Inferred from electronic annotation. Source: Ensembl

regulation of mitochondrial membrane potential

Inferred from mutant phenotype PubMed 22736029. Source: ParkinsonsUK-UCL

response to fatty acid

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from electronic annotation. Source: Ensembl

response to superoxide

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

mitochondrial inner membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from direct assay PubMed 14651853PubMed 18614015. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Mitochondrial uncoupling protein 2
PRO_0000090665

Regions

Topological domain1 – 1010Mitochondrial matrix Potential
Transmembrane11 – 3222Helical; Name=1; Potential
Topological domain33 – 7745Mitochondrial intermembrane Potential
Transmembrane78 – 10023Helical; Name=2; Potential
Topological domain101 – 11919Mitochondrial matrix Potential
Transmembrane120 – 13617Helical; Name=3; Potential
Topological domain137 – 18044Mitochondrial intermembrane Potential
Transmembrane181 – 19717Helical; Name=4; Potential
Topological domain198 – 21417Mitochondrial matrix Potential
Transmembrane215 – 23420Helical; Name=5; Potential
Topological domain235 – 26834Mitochondrial intermembrane Potential
Transmembrane269 – 29123Helical; Name=6; Potential
Topological domain292 – 30918Mitochondrial matrix Potential
Repeat11 – 10696Solcar 1
Repeat114 – 20390Solcar 2
Repeat212 – 29786Solcar 3
Region276 – 29823Purine nucleotide binding By similarity

Experimental info

Sequence conflict2851V → I in BAA32532. Ref.3

Secondary structure

...................................... 309
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P70406 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 329794EEA99810E5

FASTA30933,374
        10         20         30         40         50         60 
MVGFKATDVP PTATVKFLGA GTAACIADLI TFPLDTAKVR LQIQGESQGL VRTAASAQYR 

        70         80         90        100        110        120 
GVLGTILTMV RTEGPRSLYN GLVAGLQRQM SFASVRIGLY DSVKQFYTKG SEHAGIGSRL 

       130        140        150        160        170        180 
LAGSTTGALA VAVAQPTDVV KVRFQAQARA GGGRRYQSTV EAYKTIAREE GIRGLWKGTS 

       190        200        210        220        230        240 
PNVARNAIVN CAELVTYDLI KDTLLKANLM TDDLPCHFTS AFGAGFCTTV IASPVDVVKT 

       250        260        270        280        290        300 
RYMNSALGQY HSAGHCALTM LRKEGPRAFY KGFMPSFLRL GSWNVVMFVT YEQLKRALMA 


AYQSREAPF 

« Hide

References

« Hide 'large scale' references
[1]Raimbault S., Bouillaud F., Ricquier D.
Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: BALB/c.
Tissue: Muscle.
[2]"Cloning and characterization of an uncoupling protein homolog: a potential molecular mediator of human thermogenesis."
Gimeno R.E., Dembski M., Weng X., Deng N., Shyjan A.W., Gimeno C.J., Iris F., Ellis S.J., Woolf E.A., Tartaglia L.A.
Diabetes 46:900-906(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Spleen.
[3]"Genomic organization and promoter function of the mouse uncoupling protein 2 (UCP2) gene."
Yamada M., Hashida T., Shibusawa N., Iwasaki T., Murakami M., Monden T., Satoh T., Mori M.
FEBS Lett. 432:65-69(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C57BL/6 X CBA.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Colon and Mammary gland.
[5]"Mitochondrial uncoupling protein 2 structure determined by NMR molecular fragment searching."
Berardi M.J., Shih W.M., Harrison S.C., Chou J.J.
Nature 476:109-113(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 14-309, SUBCELLULAR LOCATION, TOPOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U69135 mRNA. Translation: AAB17666.1.
U94593 mRNA. Translation: AAB53092.1.
AB012159 Genomic DNA. Translation: BAA32532.1.
BC012697 mRNA. Translation: AAH12697.1.
BC012967 mRNA. Translation: AAH12967.1.
RefSeqNP_035801.3. NM_011671.4.
XP_006507642.1. XM_006507579.1.
UniGeneMm.171378.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2LCKNMR-A14-309[»]
ProteinModelPortalP70406.
SMRP70406. Positions 14-308.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP70406.

Proteomic databases

PaxDbP70406.
PRIDEP70406.

Protocols and materials databases

DNASU22228.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000126534; ENSMUSP00000120967; ENSMUSG00000033685.
GeneID22228.
KEGGmmu:22228.
UCSCuc009inb.2. mouse.

Organism-specific databases

CTD7351.
MGIMGI:109354. Ucp2.

Phylogenomic databases

eggNOGNOG300436.
HOGENOMHOG000165140.
HOVERGENHBG009528.
InParanoidP70406.
KOK15103.
OMASPNIARN.
OrthoDBEOG793B7Z.
PhylomeDBP70406.
TreeFamTF323211.

Gene expression databases

ArrayExpressP70406.
BgeeP70406.
GenevestigatorP70406.

Family and domain databases

Gene3D1.50.40.10. 1 hit.
InterProIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSPR00784. MTUNCOUPLING.
SUPFAMSSF103506. SSF103506. 1 hit.
PROSITEPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSUCP2. mouse.
NextBio302263.
PROP70406.
SOURCESearch...

Entry information

Entry nameUCP2_MOUSE
AccessionPrimary (citable) accession number: P70406
Secondary accession number(s): O88285
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot