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P70404

- IDHG1_MOUSE

UniProt

P70404 - IDHG1_MOUSE

Protein

Isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial

Gene

Idh3g

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Enzyme regulationi

    Activated by increasing ADP/ATP ratios and by Ca2+.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei136 – 1361SubstrateBy similarity
    Binding sitei167 – 1671SubstrateBy similarity
    Sitei174 – 1741Critical for catalysisBy similarity
    Sitei221 – 2211Critical for catalysisBy similarity
    Metal bindingi254 – 2541Magnesium or manganeseBy similarity
    Binding sitei254 – 2541SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi56 – 8429NADSequence AnalysisAdd
    BLAST
    Nucleotide bindingi309 – 3168ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB
    3. magnesium ion binding Source: InterPro
    4. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: UniProtKB
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, NAD, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial (EC:1.1.1.41)
    Alternative name(s):
    Isocitric dehydrogenase subunit gamma
    NAD(+)-specific ICDH subunit gamma
    Gene namesi
    Name:Idh3g
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1099463. Idh3g.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB
    2. nucleolus Source: Ensembl

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3939MitochondrionBy similarityAdd
    BLAST
    Chaini40 – 393354Isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrialPRO_0000014451Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei206 – 2061N6-acetyllysine1 Publication
    Modified residuei226 – 2261N6-succinyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP70404.
    PaxDbiP70404.
    PRIDEiP70404.

    PTM databases

    PhosphoSiteiP70404.

    Expressioni

    Gene expression databases

    ArrayExpressiP70404.
    BgeeiP70404.
    CleanExiMM_IDH3G.
    GenevestigatoriP70404.

    Interactioni

    Subunit structurei

    Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

    Protein-protein interaction databases

    BioGridi200511. 1 interaction.
    IntActiP70404. 4 interactions.
    MINTiMINT-1861482.

    Structurei

    3D structure databases

    ProteinModelPortaliP70404.
    SMRiP70404. Positions 50-383.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0473.
    HOVERGENiHBG052080.
    InParanoidiP70404.
    KOiK00030.
    OMAiNETRLHT.
    OrthoDBiEOG75B85R.
    PhylomeDBiP70404.
    TreeFamiTF315033.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P70404-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALKVAIAAG GAAKAMLKPT LLCRPWEVLA AHVAPRRSIS SQQTIPPSAK    50
    YGGRHTVTMI PGDGIGPELM LHVKSVFRHA CVPVDFEEVH VSSNADEEDI 100
    RNAIMAIRRN RVALKGNIET NHNLPPSHKS RNNILRTSLD LYANVIHCKS 150
    LPGVVTRHKD IDILIVRENT EGEYSSLEHE SVAGVVESLK IITKAKSLRI 200
    AEYAFKLAQE SGRKKVTAVH KANIMKLGDG LFLQCCREVA AHYPQITFDS 250
    MIVDNTTMQL VSRPQQFDVM VMPNLYGNIV NNVCAGLVGG PGLVAGANYG 300
    HVYAVFETAT RNTGKSIANK NIANPTATLL ASCMMLDHLK LHSYATSIRK 350
    AVLASMDNEN MHTPDIGGQG TTSQAIQDII RHIRIINGRA VEA 393
    Length:393
    Mass (Da):42,785
    Last modified:February 1, 1997 - v1
    Checksum:i4023560C44C1F4D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U68564 mRNA. Translation: AAC53340.1.
    AF133093 Genomic DNA. No translation available.
    CCDSiCCDS30212.1.
    RefSeqiNP_032349.1. NM_008323.1.
    UniGeneiMm.14825.

    Genome annotation databases

    EnsembliENSMUST00000052761; ENSMUSP00000056502; ENSMUSG00000002010.
    GeneIDi15929.
    KEGGimmu:15929.
    UCSCiuc009tmp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U68564 mRNA. Translation: AAC53340.1 .
    AF133093 Genomic DNA. No translation available.
    CCDSi CCDS30212.1.
    RefSeqi NP_032349.1. NM_008323.1.
    UniGenei Mm.14825.

    3D structure databases

    ProteinModelPortali P70404.
    SMRi P70404. Positions 50-383.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200511. 1 interaction.
    IntActi P70404. 4 interactions.
    MINTi MINT-1861482.

    PTM databases

    PhosphoSitei P70404.

    Proteomic databases

    MaxQBi P70404.
    PaxDbi P70404.
    PRIDEi P70404.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000052761 ; ENSMUSP00000056502 ; ENSMUSG00000002010 .
    GeneIDi 15929.
    KEGGi mmu:15929.
    UCSCi uc009tmp.1. mouse.

    Organism-specific databases

    CTDi 3421.
    MGIi MGI:1099463. Idh3g.

    Phylogenomic databases

    eggNOGi COG0473.
    HOVERGENi HBG052080.
    InParanoidi P70404.
    KOi K00030.
    OMAi NETRLHT.
    OrthoDBi EOG75B85R.
    PhylomeDBi P70404.
    TreeFami TF315033.

    Miscellaneous databases

    NextBioi 288644.
    PROi P70404.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70404.
    Bgeei P70404.
    CleanExi MM_IDH3G.
    Genevestigatori P70404.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic organization of two novel genes on human Xq28: compact head to head arrangement of IDH gamma and TRAP delta is conserved in rat and mouse."
      Brenner V., Nyakatura G., Rosenthal A., Platzer M.
      Genomics 44:8-14(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Comparative sequence analysis of the mouse L1cam locus and the corresponding region of human Xq28."
      Platzer M., Brenner V., Reichwald K., Wiehe T., Oksche A., Rosenthal A.
      Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 70-108; 116-129; 137-190 AND 227-237, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6 and OF1.
      Tissue: Brain and Hippocampus.
    4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiIDHG1_MOUSE
    AccessioniPrimary (citable) accession number: P70404
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3