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Protein

Isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial

Gene

Idh3g

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.By similarity

Enzyme regulationi

Activated by increasing ADP/ATP ratios and by Ca2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei136 – 1361SubstrateBy similarity
Binding sitei167 – 1671SubstrateBy similarity
Sitei174 – 1741Critical for catalysisBy similarity
Sitei221 – 2211Critical for catalysisBy similarity
Metal bindingi254 – 2541Magnesium or manganeseBy similarity
Binding sitei254 – 2541SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi56 – 8429NADSequence AnalysisAdd
BLAST
Nucleotide bindingi309 – 3168ATPSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_333358. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial (EC:1.1.1.41)
Alternative name(s):
Isocitric dehydrogenase subunit gamma
NAD(+)-specific ICDH subunit gamma
Gene namesi
Name:Idh3g
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1099463. Idh3g.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939MitochondrionBy similarityAdd
BLAST
Chaini40 – 393354Isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrialPRO_0000014451Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei206 – 2061N6-acetyllysine1 Publication
Modified residuei226 – 2261N6-succinyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP70404.
PaxDbiP70404.
PRIDEiP70404.

PTM databases

PhosphoSiteiP70404.

Expressioni

Gene expression databases

BgeeiP70404.
CleanExiMM_IDH3G.
ExpressionAtlasiP70404. baseline and differential.
GenevisibleiP70404. MM.

Interactioni

Subunit structurei

Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

Protein-protein interaction databases

BioGridi200511. 1 interaction.
IntActiP70404. 4 interactions.
MINTiMINT-1861482.
STRINGi10090.ENSMUSP00000056502.

Structurei

3D structure databases

ProteinModelPortaliP70404.
SMRiP70404. Positions 50-383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
GeneTreeiENSGT00590000083091.
HOVERGENiHBG052080.
InParanoidiP70404.
KOiK00030.
OMAiKRVNWPH.
OrthoDBiEOG75B85R.
PhylomeDBiP70404.
TreeFamiTF315033.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70404-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKVAIAAG GAAKAMLKPT LLCRPWEVLA AHVAPRRSIS SQQTIPPSAK
60 70 80 90 100
YGGRHTVTMI PGDGIGPELM LHVKSVFRHA CVPVDFEEVH VSSNADEEDI
110 120 130 140 150
RNAIMAIRRN RVALKGNIET NHNLPPSHKS RNNILRTSLD LYANVIHCKS
160 170 180 190 200
LPGVVTRHKD IDILIVRENT EGEYSSLEHE SVAGVVESLK IITKAKSLRI
210 220 230 240 250
AEYAFKLAQE SGRKKVTAVH KANIMKLGDG LFLQCCREVA AHYPQITFDS
260 270 280 290 300
MIVDNTTMQL VSRPQQFDVM VMPNLYGNIV NNVCAGLVGG PGLVAGANYG
310 320 330 340 350
HVYAVFETAT RNTGKSIANK NIANPTATLL ASCMMLDHLK LHSYATSIRK
360 370 380 390
AVLASMDNEN MHTPDIGGQG TTSQAIQDII RHIRIINGRA VEA
Length:393
Mass (Da):42,785
Last modified:February 1, 1997 - v1
Checksum:i4023560C44C1F4D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68564 mRNA. Translation: AAC53340.1.
AF133093 Genomic DNA. No translation available.
CCDSiCCDS30212.1.
RefSeqiNP_032349.1. NM_008323.1.
UniGeneiMm.14825.

Genome annotation databases

EnsembliENSMUST00000052761; ENSMUSP00000056502; ENSMUSG00000002010.
GeneIDi15929.
KEGGimmu:15929.
UCSCiuc009tmp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68564 mRNA. Translation: AAC53340.1.
AF133093 Genomic DNA. No translation available.
CCDSiCCDS30212.1.
RefSeqiNP_032349.1. NM_008323.1.
UniGeneiMm.14825.

3D structure databases

ProteinModelPortaliP70404.
SMRiP70404. Positions 50-383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200511. 1 interaction.
IntActiP70404. 4 interactions.
MINTiMINT-1861482.
STRINGi10090.ENSMUSP00000056502.

PTM databases

PhosphoSiteiP70404.

Proteomic databases

MaxQBiP70404.
PaxDbiP70404.
PRIDEiP70404.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052761; ENSMUSP00000056502; ENSMUSG00000002010.
GeneIDi15929.
KEGGimmu:15929.
UCSCiuc009tmp.1. mouse.

Organism-specific databases

CTDi3421.
MGIiMGI:1099463. Idh3g.

Phylogenomic databases

eggNOGiCOG0473.
GeneTreeiENSGT00590000083091.
HOVERGENiHBG052080.
InParanoidiP70404.
KOiK00030.
OMAiKRVNWPH.
OrthoDBiEOG75B85R.
PhylomeDBiP70404.
TreeFamiTF315033.

Enzyme and pathway databases

ReactomeiREACT_333358. Citric acid cycle (TCA cycle).

Miscellaneous databases

ChiTaRSiIdh3g. mouse.
NextBioi288644.
PROiP70404.
SOURCEiSearch...

Gene expression databases

BgeeiP70404.
CleanExiMM_IDH3G.
ExpressionAtlasiP70404. baseline and differential.
GenevisibleiP70404. MM.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization of two novel genes on human Xq28: compact head to head arrangement of IDH gamma and TRAP delta is conserved in rat and mouse."
    Brenner V., Nyakatura G., Rosenthal A., Platzer M.
    Genomics 44:8-14(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Comparative sequence analysis of the mouse L1cam locus and the corresponding region of human Xq28."
    Platzer M., Brenner V., Reichwald K., Wiehe T., Oksche A., Rosenthal A.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 70-108; 116-129; 137-190 AND 227-237, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiIDHG1_MOUSE
AccessioniPrimary (citable) accession number: P70404
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 22, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.