UniProtKB - P70396 (DLX5_MOUSE)
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Protein
Homeobox protein DLX-5
Gene
Dlx5
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding.3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 137 – 196 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA binding Source: MGI
- HMG box domain binding Source: UniProtKB
- RNA polymerase II proximal promoter sequence-specific DNA binding Source: BHF-UCL
- transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: BHF-UCL
- transcription regulatory region DNA binding Source: UniProtKB
GO - Biological processi
- anatomical structure formation involved in morphogenesis Source: MGI
- axon guidance Source: MGI
- axonogenesis Source: MGI
- BMP signaling pathway Source: BHF-UCL
- bone morphogenesis Source: MGI
- cell proliferation Source: UniProtKB
- cellular response to BMP stimulus Source: BHF-UCL
- ear development Source: MGI
- embryonic limb morphogenesis Source: MGI
- endochondral ossification Source: UniProtKB
- epithelial cell differentiation Source: MGI
- face morphogenesis Source: MGI
- head development Source: MGI
- inner ear morphogenesis Source: MGI
- interneuron axon guidance Source: MGI
- olfactory bulb interneuron differentiation Source: MGI
- olfactory pit development Source: MGI
- osteoblast differentiation Source: UniProtKB
- palate development Source: MGI
- positive regulation of canonical Wnt signaling pathway Source: MGI
- positive regulation of epithelial cell proliferation Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus Source: BHF-UCL
- regulation of transcription, DNA-templated Source: MGI
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Osteogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Homeobox protein DLX-5 |
Gene namesi | Name:Dlx5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101926 Dlx5 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 34 | S → A: Inhibits its transcriptional activity; when associated with A-217. 1 Publication | 1 | |
Mutagenesisi | 217 | S → A: Inhibits its transcriptional activity; when associated with A-34. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049032 | 1 – 289 | Homeobox protein DLX-5Add BLAST | 289 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 34 | Phosphoserine; by MAPK14; in vitro1 Publication | 1 | |
Modified residuei | 217 | Phosphoserine; by MAPK14; in vitro1 Publication | 1 |
Post-translational modificationi
Phosphorylated. Phosphorylation of Ser-34 and Ser-217 by MAPK14 enhances its transcriptional activity. Phosphorylation by CaMK2 increases its protein stability.2 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P70396 |
PRIDEi | P70396 |
PTM databases
iPTMneti | P70396 |
PhosphoSitePlusi | P70396 |
Expressioni
Tissue specificityi
Expressed in osteoblasts and chondrocytes.1 Publication
Developmental stagei
Expressed in the otic vesicle, mandibular arch, branchial arches 2 and 3, in proximal anterior mesodermal domain in the limb, immature and proliferating chondroblasts at 14.5 dpc.1 Publication
Inductioni
Up-regulated by BMP2.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000029755 |
CleanExi | MM_DLX5 |
ExpressionAtlasi | P70396 baseline and differential |
Genevisiblei | P70396 MM |
Interactioni
Subunit structurei
Interacts with XRCC6 (Ku70).By similarity
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
Maged1 | Q9QYH6 | 2 | EBI-1801294,EBI-1801274 |
GO - Molecular functioni
- HMG box domain binding Source: UniProtKB
Protein-protein interaction databases
BioGridi | 19923810 interactors. |
IntActi | P70396 1 interactor. |
STRINGi | 10090.ENSMUSP00000052559 |
Structurei
3D structure databases
ProteinModelPortali | P70396 |
SMRi | P70396 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the distal-less homeobox family.Curated
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0850 Eukaryota ENOG410ZHZR LUCA |
GeneTreei | ENSGT00910000143987 |
HOGENOMi | HOG000231940 |
HOVERGENi | HBG005493 |
InParanoidi | P70396 |
KOi | K18489 |
OMAi | NSASWYP |
OrthoDBi | EOG091G0P17 |
PhylomeDBi | P70396 |
TreeFami | TF350606 |
Family and domain databases
CDDi | cd00086 homeodomain, 1 hit |
InterProi | View protein in InterPro IPR022135 Distal-less_N IPR009057 Homeobox-like_sf IPR017970 Homeobox_CS IPR001356 Homeobox_dom IPR020479 Homeobox_metazoa IPR000047 HTH_motif |
Pfami | View protein in Pfam PF12413 DLL_N, 1 hit PF00046 Homeobox, 1 hit |
PRINTSi | PR00024 HOMEOBOX PR00031 HTHREPRESSR |
SMARTi | View protein in SMART SM00389 HOX, 1 hit |
SUPFAMi | SSF46689 SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027 HOMEOBOX_1, 1 hit PS50071 HOMEOBOX_2, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: P70396-1) [UniParc]FASTAAdd to basket
Also known as: Alpha
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MTGVFDRRVP SIRSGDFQAP FPTSAAMHHP SQESPTLPES SATDSDYYSP
60 70 80 90 100
AGAAPHGYCS PTSASYGKAL NPYQYQYHGV NGSAAGYPAK AYADYGYASP
110 120 130 140 150
YHQYGGAYNR VPSATSQPEK EVAEPEVRMV NGKPKKVRKP RTIYSSFQLA
160 170 180 190 200
ALQRRFQKTQ YLALPERAEL AASLGLTQTQ VKIWFQNKRS KIKKIMKNGE
210 220 230 240 250
MPPEHSPSSS DPMACNSPQS PAVWEPQGSS RSLSHHPHAH PPTSNQSPAS
260 270 280
SYLENSASWY PSAASSINSH LPPPGSLQHP LALASGTLY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 98 – 99 | AS → H in AAB94580 (PubMed:9415433).Curated | 2 | |
Sequence conflicti | 98 – 99 | AS → H in AAB94581 (PubMed:9415433).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002237 | 119 – 146 | EKEVA…TIYSS → AFSWPLYREGFRRLSTSPCQ NARSWPPL in isoform 3. 2 PublicationsAdd BLAST | 28 | |
Alternative sequenceiVSP_002238 | 147 – 289 | Missing in isoform 3. 2 PublicationsAdd BLAST | 143 | |
Alternative sequenceiVSP_002239 | 147 – 251 | Missing in isoform 2. 1 PublicationAdd BLAST | 105 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U67840 mRNA Translation: AAC52843.1 AF033011 mRNA Translation: AAB86899.1 AF022075 mRNA Translation: AAB94580.1 AF022076 mRNA Translation: AAB94581.1 AF022077 mRNA Translation: AAB94582.1 AF072452 mRNA Translation: AAF86636.1 AF072453 mRNA Translation: AAF86637.1 |
CCDSi | CCDS19905.1 [P70396-1] CCDS59691.1 [P70396-3] |
RefSeqi | NP_034186.2, NM_010056.3 [P70396-1] NP_942151.1, NM_198854.2 [P70396-3] |
UniGenei | Mm.4873 |
Genome annotation databases
Ensembli | ENSMUST00000052609; ENSMUSP00000052559; ENSMUSG00000029755 [P70396-1] ENSMUST00000142635; ENSMUSP00000138264; ENSMUSG00000029755 [P70396-3] |
GeneIDi | 13395 |
KEGGi | mmu:13395 |
UCSCi | uc009awy.2 mouse [P70396-3] uc009awz.2 mouse [P70396-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | DLX5_MOUSE | |
Accessioni | P70396Primary (citable) accession number: P70396 Secondary accession number(s): O54876 Q9JJ45 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | March 28, 2018 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |