Reviewed, UniProtKB/Swiss-Prot P70396 (DLX5_MOUSE)

Names and origin

Protein names

Recommended name:
    Homeobox protein DLX-5

Gene names

Name:

Dlx5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in craniofacial patterning and morphogenesis.
Subunit structure	Interacts with G22P1 (Ku70) By similarity.
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the distal-less homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Gene Ontology (GO)
Biological process	anatomical structure formation involved in morphogenesis Inferred from genetic interaction. Source: MGI axonogenesis Inferred from mutant phenotype. Source: MGI embryonic limb morphogenesis Inferred from genetic interaction. Source: MGI epithelial cell differentiation Inferred from genetic interaction. Source: MGI inner ear morphogenesis Inferred from mutant phenotype. Source: MGI positive regulation of epithelial cell proliferation Inferred from genetic interaction. Source: MGI regulation of transcription, DNA-dependent Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Maged1	Q9QYH6	1	EBI-1801294,EBI-1801274

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P70396-1)

Also known as: Alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P70396-2)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     147-251: Missing.

Isoform 3 (identifier: P70396-3)

Also known as: Gamma;

The sequence of this isoform differs from the canonical sequence as follows:
     119-146: EKEVAEPEVRMVNGKPKKVRKPRTIYSS → AFSWPLYREGFRRLSTSPCQNARSWPPL
     147-289: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Homeobox protein DLX-5		PRO_0000049032
Regions
	DNA binding	137 – 196	60	Homeobox
Natural variations
	Alternative sequence	119 – 146	28	EKEVA…TIYSS → AFSWPLYREGFRRLSTSPCQ NARSWPPL in isoform 3.		VSP_002237
	Alternative sequence	147 – 289	143	Missing in isoform 3.		VSP_002238
	Alternative sequence	147 – 251	105	Missing in isoform 2.		VSP_002239
Experimental info
	Sequence conflict	98 – 99	2	AS → H in AAB94580. Ref.3
	Sequence conflict	98 – 99	2	AS → H in AAB94581. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Alpha) [UniParc]. Last modified February 1, 1997. Version 1. Checksum: C53555610E9F8777	FASTA	289	31,396
	10 20 30 40 50 60 MTGVFDRRVP SIRSGDFQAP FPTSAAMHHP SQESPTLPES SATDSDYYSP AGAAPHGYCS 70 80 90 100 110 120 PTSASYGKAL NPYQYQYHGV NGSAAGYPAK AYADYGYASP YHQYGGAYNR VPSATSQPEK 130 140 150 160 170 180 EVAEPEVRMV NGKPKKVRKP RTIYSSFQLA ALQRRFQKTQ YLALPERAEL AASLGLTQTQ 190 200 210 220 230 240 VKIWFQNKRS KIKKIMKNGE MPPEHSPSSS DPMACNSPQS PAVWEPQGSS RSLSHHPHAH 250 260 270 280 PPTSNQSPAS SYLENSASWY PSAASSINSH LPPPGSLQHP LALASGTLY
	Isoform 2 (Beta). Checksum: C96A32599A6AD931	FASTA	184	19,772
	10 20 30 40 50 60 MTGVFDRRVP SIRSGDFQAP FPTSAAMHHP SQESPTLPES SATDSDYYSP AGAAPHGYCS 70 80 90 100 110 120 PTSASYGKAL NPYQYQYHGV NGSAAGYPAK AYADYGYASP YHQYGGAYNR VPSATSQPEK 130 140 150 160 170 180 EVAEPEVRMV NGKPKKVRKP RTIYSSYLEN SASWYPSAAS SINSHLPPPG SLQHPLALAS GTLY
	Isoform 3 (Gamma). Checksum: 55BAEA4F6C3251B6	FASTA	146	15,858
	10 20 30 40 50 60 MTGVFDRRVP SIRSGDFQAP FPTSAAMHHP SQESPTLPES SATDSDYYSP AGAAPHGYCS 70 80 90 100 110 120 PTSASYGKAL NPYQYQYHGV NGSAAGYPAK AYADYGYASP YHQYGGAYNR VPSATSQPAF 130 140 SWPLYREGFR RLSTSPCQNA RSWPPL

References

[1]	"The evolution of the vertebrate Dlx gene family." Stock D.W., Ellies D.L., Zhao Z., Ekker M., Ruddle F.H., Weiss K.M. Proc. Natl. Acad. Sci. U.S.A. 93:10858-10863(1996) [PubMed: 8855272] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: Swiss Webster.
[2]	"A BMP-inducible gene, dlx5, regulates osteoblast differentiation and mesoderm induction." Miyama K., Yamada G., Yamamoto T.S., Takagi C., Miyado K., Sakai M., Ueno N., Shibuya H. Dev. Biol. 208:123-133(1999) [PubMed: 10075846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Dlx genes encode DNA-binding proteins that are expressed in an overlapping and sequential pattern during basal ganglia differentiation." Liu J.K., Ghattas I., Liu S., Chen S., Rubenstein J.L.R. Dev. Dyn. 210:498-512(1997) [PubMed: 9415433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
[4]	"An early phase of embryonic Dlx5 expression defines the rostral boundary of the neural plate." Yang L., Zhang H., Hu G., Wang H., Abate-Shen C., Shen M.M. J. Neurosci. 18:8322-8330(1998) [PubMed: 9763476] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3). Tissue: Embryonic head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U67840 mRNA. Translation: AAC52843.1. AF033011 mRNA. Translation: AAB86899.1. AF022075 mRNA. Translation: AAB94580.1. AF022076 mRNA. Translation: AAB94581.1. AF022077 mRNA. Translation: AAB94582.1. AF072452 mRNA. Translation: AAF86636.1. AF072453 mRNA. Translation: AAF86637.1.
IPI	IPI00108386. IPI00228527. IPI00228528.
RefSeq	NP_034186.2. NP_942151.1.
UniGene	Mm.4873
3D structure databases
HSSP	HSSP built from PDB template 1IG7 based on UniProtKB P13297.
SMR	P70396. Positions 138-194.
ModBase	Search...
Protein-protein interaction databases
IntAct	P70396. 1 interaction.
PTM databases
PhosphoSite	P70396.
Genome annotation databases
Ensembl	ENSMUSG00000029755. Mus musculus. [Contig view]
GeneID	13395.
KEGG	mmu:13395.
Organism-specific databases
MGI	MGI:101926. Dlx5.
Phylogenomic databases
HOGENOM	P70396.
HOVERGEN	P70396.
OMA	P70396. SPYHQYG.
Gene expression databases
ArrayExpress	P70396.
Bgee	P70396.
CleanEx	MM_DLX5.
GermOnline	ENSMUSG00000029755. Mus musculus.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR012287. Homeodomain-rel. IPR000047. HTH_lambrepressr. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00031. HTHREPRESSR.
ProDom	PD000010. Homeobox. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00389. HOX. 1 hit. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	283772.
SOURCE	Search...

Entry information

Entry name

DLX5_MOUSE

Accession

Primary (citable) accession number: P70396
Secondary accession number(s): O54876 , O54877, O54878, Q9JJ45

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)