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Protein

Homeobox protein DLX-5

Gene

Dlx5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi137 – 196HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • DNA binding Source: MGI
  • HMG box domain binding Source: UniProtKB
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: BHF-UCL
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: BHF-UCL
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  • anatomical structure formation involved in morphogenesis Source: MGI
  • axon guidance Source: MGI
  • axonogenesis Source: MGI
  • BMP signaling pathway Source: BHF-UCL
  • bone morphogenesis Source: MGI
  • cell proliferation Source: UniProtKB
  • cellular response to BMP stimulus Source: BHF-UCL
  • ear development Source: MGI
  • embryonic limb morphogenesis Source: MGI
  • endochondral ossification Source: UniProtKB
  • epithelial cell differentiation Source: MGI
  • face morphogenesis Source: MGI
  • head development Source: MGI
  • inner ear morphogenesis Source: MGI
  • interneuron axon guidance Source: MGI
  • olfactory bulb interneuron differentiation Source: MGI
  • olfactory pit development Source: MGI
  • osteoblast differentiation Source: UniProtKB
  • palate development Source: MGI
  • positive regulation of canonical Wnt signaling pathway Source: MGI
  • positive regulation of epithelial cell proliferation Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus Source: BHF-UCL
  • regulation of transcription, DNA-templated Source: MGI

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processOsteogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein DLX-5
Gene namesi
Name:Dlx5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:101926. Dlx5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi34S → A: Inhibits its transcriptional activity; when associated with A-217. 1 Publication1
Mutagenesisi217S → A: Inhibits its transcriptional activity; when associated with A-34. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000490321 – 289Homeobox protein DLX-5Add BLAST289

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34Phosphoserine; by MAPK14; in vitro1 Publication1
Modified residuei217Phosphoserine; by MAPK14; in vitro1 Publication1

Post-translational modificationi

Phosphorylated. Phosphorylation of Ser-34 and Ser-217 by MAPK14 enhances its transcriptional activity. Phosphorylation by CaMK2 increases its protein stability.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP70396.
PRIDEiP70396.

PTM databases

iPTMnetiP70396.
PhosphoSitePlusiP70396.

Expressioni

Tissue specificityi

Expressed in osteoblasts and chondrocytes.1 Publication

Developmental stagei

Expressed in the otic vesicle, mandibular arch, branchial arches 2 and 3, in proximal anterior mesodermal domain in the limb, immature and proliferating chondroblasts at 14.5 dpc.1 Publication

Inductioni

Up-regulated by BMP2.2 Publications

Gene expression databases

BgeeiENSMUSG00000029755.
CleanExiMM_DLX5.
ExpressionAtlasiP70396. baseline and differential.
GenevisibleiP70396. MM.

Interactioni

Subunit structurei

Interacts with XRCC6 (Ku70).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Maged1Q9QYH62EBI-1801294,EBI-1801274

GO - Molecular functioni

  • HMG box domain binding Source: UniProtKB

Protein-protein interaction databases

BioGridi199238. 10 interactors.
IntActiP70396. 1 interactor.
STRINGi10090.ENSMUSP00000052559.

Structurei

3D structure databases

ProteinModelPortaliP70396.
SMRiP70396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the distal-less homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0850. Eukaryota.
ENOG410ZHZR. LUCA.
GeneTreeiENSGT00900000140771.
HOGENOMiHOG000231940.
HOVERGENiHBG005493.
InParanoidiP70396.
KOiK18489.
OMAiNSASWYP.
OrthoDBiEOG091G0P17.
PhylomeDBiP70396.
TreeFamiTF350606.

Family and domain databases

CDDicd00086. homeodomain. 1 hit.
InterProiView protein in InterPro
IPR022135. Distal-less_N.
IPR009057. Homeobox-like_sf.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR000047. HTH_motif.
PfamiView protein in Pfam
PF12413. DLL_N. 1 hit.
PF00046. Homeobox. 1 hit.
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiView protein in PROSITE
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P70396-1) [UniParc]FASTAAdd to basket
Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTGVFDRRVP SIRSGDFQAP FPTSAAMHHP SQESPTLPES SATDSDYYSP
60 70 80 90 100
AGAAPHGYCS PTSASYGKAL NPYQYQYHGV NGSAAGYPAK AYADYGYASP
110 120 130 140 150
YHQYGGAYNR VPSATSQPEK EVAEPEVRMV NGKPKKVRKP RTIYSSFQLA
160 170 180 190 200
ALQRRFQKTQ YLALPERAEL AASLGLTQTQ VKIWFQNKRS KIKKIMKNGE
210 220 230 240 250
MPPEHSPSSS DPMACNSPQS PAVWEPQGSS RSLSHHPHAH PPTSNQSPAS
260 270 280
SYLENSASWY PSAASSINSH LPPPGSLQHP LALASGTLY
Length:289
Mass (Da):31,396
Last modified:February 1, 1997 - v1
Checksum:iC53555610E9F8777
GO
Isoform 2 (identifier: P70396-2) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     147-251: Missing.

Show »
Length:184
Mass (Da):19,772
Checksum:iC96A32599A6AD931
GO
Isoform 3 (identifier: P70396-3) [UniParc]FASTAAdd to basket
Also known as: Gamma

The sequence of this isoform differs from the canonical sequence as follows:
     119-146: EKEVAEPEVRMVNGKPKKVRKPRTIYSS → AFSWPLYREGFRRLSTSPCQNARSWPPL
     147-289: Missing.

Show »
Length:146
Mass (Da):15,858
Checksum:i55BAEA4F6C3251B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti98 – 99AS → H in AAB94580 (PubMed:9415433).Curated2
Sequence conflicti98 – 99AS → H in AAB94581 (PubMed:9415433).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002237119 – 146EKEVA…TIYSS → AFSWPLYREGFRRLSTSPCQ NARSWPPL in isoform 3. 2 PublicationsAdd BLAST28
Alternative sequenceiVSP_002238147 – 289Missing in isoform 3. 2 PublicationsAdd BLAST143
Alternative sequenceiVSP_002239147 – 251Missing in isoform 2. 1 PublicationAdd BLAST105

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67840 mRNA. Translation: AAC52843.1.
AF033011 mRNA. Translation: AAB86899.1.
AF022075 mRNA. Translation: AAB94580.1.
AF022076 mRNA. Translation: AAB94581.1.
AF022077 mRNA. Translation: AAB94582.1.
AF072452 mRNA. Translation: AAF86636.1.
AF072453 mRNA. Translation: AAF86637.1.
CCDSiCCDS19905.1. [P70396-1]
CCDS59691.1. [P70396-3]
RefSeqiNP_034186.2. NM_010056.3. [P70396-1]
NP_942151.1. NM_198854.2. [P70396-3]
UniGeneiMm.4873.

Genome annotation databases

EnsembliENSMUST00000052609; ENSMUSP00000052559; ENSMUSG00000029755. [P70396-1]
ENSMUST00000142635; ENSMUSP00000138264; ENSMUSG00000029755. [P70396-3]
GeneIDi13395.
KEGGimmu:13395.
UCSCiuc009awy.2. mouse. [P70396-3]
uc009awz.2. mouse. [P70396-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDLX5_MOUSE
AccessioniPrimary (citable) accession number: P70396
Secondary accession number(s): O54876
, O54877, O54878, Q9JJ45
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 22, 2017
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families