SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P70396

- DLX5_MOUSE

UniProt

P70396 - DLX5_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Homeobox protein DLX-5
Gene
Dlx5
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi137 – 19660Homeobox1 Publication
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. HMG box domain binding Source: UniProtKB
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: BHF-UCL
  4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: BHF-UCL
  5. protein binding Source: IntAct
  6. transcription regulatory region DNA binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. BMP signaling pathway Source: BHF-UCL
  2. anatomical structure formation involved in morphogenesis Source: MGI
  3. axon guidance Source: MGI
  4. axonogenesis Source: MGI
  5. bone morphogenesis Source: MGI
  6. cell proliferation Source: UniProtKB
  7. cellular response to BMP stimulus Source: BHF-UCL
  8. ear development Source: MGI
  9. embryonic limb morphogenesis Source: MGI
  10. endochondral ossification Source: UniProtKB
  11. epithelial cell differentiation Source: MGI
  12. face morphogenesis Source: MGI
  13. head development Source: MGI
  14. inner ear morphogenesis Source: MGI
  15. olfactory pit development Source: MGI
  16. osteoblast differentiation Source: UniProtKB
  17. palate development Source: MGI
  18. positive regulation of canonical Wnt signaling pathway Source: MGI
  19. positive regulation of epithelial cell proliferation Source: MGI
  20. positive regulation of osteoblast differentiation Source: Ensembl
  21. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  22. positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus Source: BHF-UCL
  23. positive regulation of transcription, DNA-templated Source: UniProtKB
  24. regulation of transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Osteogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein DLX-5
Gene namesi
Name:Dlx5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:101926. Dlx5.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nuclear chromatin Source: BHF-UCL
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi34 – 341S → A: Inhibits its transcriptional activity; when associated with A-217. 1 Publication
Mutagenesisi217 – 2171S → A: Inhibits its transcriptional activity; when associated with A-34. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289Homeobox protein DLX-5
PRO_0000049032Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341Phosphoserine; by MAPK14; in vitro1 Publication
Modified residuei217 – 2171Phosphoserine; by MAPK14; in vitro1 Publication

Post-translational modificationi

Phosphorylated. Phosphorylation of Ser-34 and Ser-217 by MAPK14 enhances its transcriptional activity. Phosphorylation by CaMK2 increases its protein stability.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP70396.

PTM databases

PhosphoSiteiP70396.

Expressioni

Tissue specificityi

Expressed in osteoblasts and chondrocytes.1 Publication

Developmental stagei

Expressed in the otic vesicle, mandibular arch, branchial arches 2 and 3, in proximal anterior mesodermal domain in the limb, immature and proliferating chondroblasts at 14.5 dpc.1 Publication

Inductioni

Up-regulated by BMP2.2 Publications

Gene expression databases

ArrayExpressiP70396.
BgeeiP70396.
CleanExiMM_DLX5.
GenevestigatoriP70396.

Interactioni

Subunit structurei

Interacts with XRCC6 (Ku70) By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Maged1Q9QYH62EBI-1801294,EBI-1801274

Protein-protein interaction databases

BioGridi199238. 11 interactions.
IntActiP70396. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP70396.
SMRiP70396. Positions 138-194.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG263092.
GeneTreeiENSGT00720000108450.
HOGENOMiHOG000231940.
HOVERGENiHBG005493.
InParanoidiP70396.
KOiK09315.
OMAiQSPAVWD.
OrthoDBiEOG70W3F8.
PhylomeDBiP70396.
TreeFamiTF350606.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR022135. Distal-less_N.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamiPF12413. DLL_N. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P70396-1) [UniParc]FASTAAdd to Basket

Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTGVFDRRVP SIRSGDFQAP FPTSAAMHHP SQESPTLPES SATDSDYYSP    50
AGAAPHGYCS PTSASYGKAL NPYQYQYHGV NGSAAGYPAK AYADYGYASP 100
YHQYGGAYNR VPSATSQPEK EVAEPEVRMV NGKPKKVRKP RTIYSSFQLA 150
ALQRRFQKTQ YLALPERAEL AASLGLTQTQ VKIWFQNKRS KIKKIMKNGE 200
MPPEHSPSSS DPMACNSPQS PAVWEPQGSS RSLSHHPHAH PPTSNQSPAS 250
SYLENSASWY PSAASSINSH LPPPGSLQHP LALASGTLY 289
Length:289
Mass (Da):31,396
Last modified:February 1, 1997 - v1
Checksum:iC53555610E9F8777
GO
Isoform 2 (identifier: P70396-2) [UniParc]FASTAAdd to Basket

Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     147-251: Missing.

Show »
Length:184
Mass (Da):19,772
Checksum:iC96A32599A6AD931
GO
Isoform 3 (identifier: P70396-3) [UniParc]FASTAAdd to Basket

Also known as: Gamma

The sequence of this isoform differs from the canonical sequence as follows:
     119-146: EKEVAEPEVRMVNGKPKKVRKPRTIYSS → AFSWPLYREGFRRLSTSPCQNARSWPPL
     147-289: Missing.

Show »
Length:146
Mass (Da):15,858
Checksum:i55BAEA4F6C3251B6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei119 – 14628EKEVA…TIYSS → AFSWPLYREGFRRLSTSPCQ NARSWPPL in isoform 3.
VSP_002237Add
BLAST
Alternative sequencei147 – 289143Missing in isoform 3.
VSP_002238Add
BLAST
Alternative sequencei147 – 251105Missing in isoform 2.
VSP_002239Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti98 – 992AS → H in AAB94580. 1 Publication
Sequence conflicti98 – 992AS → H in AAB94581. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U67840 mRNA. Translation: AAC52843.1.
AF033011 mRNA. Translation: AAB86899.1.
AF022075 mRNA. Translation: AAB94580.1.
AF022076 mRNA. Translation: AAB94581.1.
AF022077 mRNA. Translation: AAB94582.1.
AF072452 mRNA. Translation: AAF86636.1.
AF072453 mRNA. Translation: AAF86637.1.
CCDSiCCDS19905.1. [P70396-1]
CCDS59691.1. [P70396-3]
RefSeqiNP_034186.2. NM_010056.3. [P70396-1]
NP_942151.1. NM_198854.2. [P70396-3]
UniGeneiMm.4873.

Genome annotation databases

EnsembliENSMUST00000052609; ENSMUSP00000052559; ENSMUSG00000029755. [P70396-1]
ENSMUST00000142635; ENSMUSP00000138264; ENSMUSG00000029755. [P70396-3]
GeneIDi13395.
KEGGimmu:13395.
UCSCiuc009awy.1. mouse. [P70396-3]
uc009awz.1. mouse. [P70396-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U67840 mRNA. Translation: AAC52843.1 .
AF033011 mRNA. Translation: AAB86899.1 .
AF022075 mRNA. Translation: AAB94580.1 .
AF022076 mRNA. Translation: AAB94581.1 .
AF022077 mRNA. Translation: AAB94582.1 .
AF072452 mRNA. Translation: AAF86636.1 .
AF072453 mRNA. Translation: AAF86637.1 .
CCDSi CCDS19905.1. [P70396-1 ]
CCDS59691.1. [P70396-3 ]
RefSeqi NP_034186.2. NM_010056.3. [P70396-1 ]
NP_942151.1. NM_198854.2. [P70396-3 ]
UniGenei Mm.4873.

3D structure databases

ProteinModelPortali P70396.
SMRi P70396. Positions 138-194.
ModBasei Search...

Protein-protein interaction databases

BioGridi 199238. 11 interactions.
IntActi P70396. 1 interaction.

PTM databases

PhosphoSitei P70396.

Proteomic databases

PRIDEi P70396.

Protocols and materials databases

DNASUi 13395.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000052609 ; ENSMUSP00000052559 ; ENSMUSG00000029755 . [P70396-1 ]
ENSMUST00000142635 ; ENSMUSP00000138264 ; ENSMUSG00000029755 . [P70396-3 ]
GeneIDi 13395.
KEGGi mmu:13395.
UCSCi uc009awy.1. mouse. [P70396-3 ]
uc009awz.1. mouse. [P70396-1 ]

Organism-specific databases

CTDi 1749.
MGIi MGI:101926. Dlx5.

Phylogenomic databases

eggNOGi NOG263092.
GeneTreei ENSGT00720000108450.
HOGENOMi HOG000231940.
HOVERGENi HBG005493.
InParanoidi P70396.
KOi K09315.
OMAi QSPAVWD.
OrthoDBi EOG70W3F8.
PhylomeDBi P70396.
TreeFami TF350606.

Miscellaneous databases

NextBioi 283772.
PROi P70396.
SOURCEi Search...

Gene expression databases

ArrayExpressi P70396.
Bgeei P70396.
CleanExi MM_DLX5.
Genevestigatori P70396.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR022135. Distal-less_N.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view ]
Pfami PF12413. DLL_N. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view ]
PRINTSi PR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTi SM00389. HOX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Swiss Webster.
  2. "A BMP-inducible gene, dlx5, regulates osteoblast differentiation and mesoderm induction."
    Miyama K., Yamada G., Yamamoto T.S., Takagi C., Miyado K., Sakai M., Ueno N., Shibuya H.
    Dev. Biol. 208:123-133(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Dlx genes encode DNA-binding proteins that are expressed in an overlapping and sequential pattern during basal ganglia differentiation."
    Liu J.K., Ghattas I., Liu S., Chen S., Rubenstein J.L.R.
    Dev. Dyn. 210:498-512(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
  4. "An early phase of embryonic Dlx5 expression defines the rostral boundary of the neural plate."
    Yang L., Zhang H., Hu G., Wang H., Abate-Shen C., Shen M.M.
    J. Neurosci. 18:8322-8330(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3).
    Tissue: Embryonic head.
  5. "Bone morphogenetic protein-2-induced alkaline phosphatase expression is stimulated by Dlx5 and repressed by Msx2."
    Kim Y.J., Lee M.H., Wozney J.M., Cho J.Y., Ryoo H.M.
    J. Biol. Chem. 279:50773-50780(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, INDUCTION.
  6. "BMP-2 induces Osterix expression through up-regulation of Dlx5 and its phosphorylation by p38."
    Ulsamer A., Ortuno M.J., Ruiz S., Susperregui A.R., Osses N., Rosa J.L., Ventura F.
    J. Biol. Chem. 283:3816-3826(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT SER-34 AND SER-217, MUTAGENESIS OF SER-34 AND SER-217, SUBCELLULAR LOCATION, INDUCTION.
  7. "Calmodulin-dependent kinase II regulates Dlx5 during osteoblast differentiation."
    Seo J.H., Jin Y.H., Jeong H.M., Kim Y.J., Jeong H.G., Yeo C.Y., Lee K.Y.
    Biochem. Biophys. Res. Commun. 384:100-104(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  8. "Dlx5 Is a cell autonomous regulator of chondrocyte hypertrophy in mice and functionally substitutes for Dlx6 during endochondral ossification."
    Zhu H., Bendall A.J.
    PLoS ONE 4:E8097-E8097(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiDLX5_MOUSE
AccessioniPrimary (citable) accession number: P70396
Secondary accession number(s): O54876
, O54877, O54878, Q9JJ45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi