Reviewed, UniProtKB/Swiss-Prot P70385 (DHB3_MOUSE)

Names and origin

Protein names

Recommended name:
    Testosterone 17-beta-dehydrogenase 3
    EC=1.1.1.64
Alternative name(s):
    Testicular 17-beta-hydroxysteroid dehydrogenase
    17-beta-HSD 3

Gene names

Name:	Hsd17b3
Synonyms:	Edh17b3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH By similarity.
Catalytic activity	Testosterone + NADP(+) = androst-4-ene-3,17-dione + NADPH.
Pathway	Hormone biosynthesis; testosterone biosynthesis.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro testosterone 17-beta-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Testosterone 17-beta-dehydrogenase 3		PRO_0000054574
Regions
	Nucleotide binding	44 – 73	30	NADP By similarity
Sites
	Active site	194	1	Proton acceptor By similarity
	Binding site	181	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70385-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 183860C85213EB61	FASTA	305	34,271
	10 20 30 40 50 60 MEKLFIAAGL FVGLVCLVKC MRFSQHLFLR FCKALPSSFL RSMGQWAVIT GAGDGIGKAY 70 80 90 100 110 120 SFELARHGLN VVLISRTLEK LQTIAEEIER TTGSGVKIVQ ADFTREDIYD HIKEHLEGLE 130 140 150 160 170 180 NGILVNNVGM LPSFFPSHFL SSSGESQNLI HCNITSVVKM TQLVLKHMES RRKGLILNIS 190 200 210 220 230 240 SGAALRPWPL YSLYSASKAF VYTFSKALSV EYRDKGIIIQ VLTPYSISTP MTKYLNNKMT 250 260 270 280 290 300 KTADEFVKES LKYVTIGAES CGCLAHEIIA IILNRIPSRI FYSSTAQRFL LTRYSDYLKR NISNR

References

[1]	"Sequence of mouse 17beta-hydroxysteroid dehydrogenase type 3 cDNA and tissue distribution of the type 1 and type 3 isoform mRNAs." Sha J.A., Dudley K., Rajapaksha W.R.A.K.J.S., O'Shaughnessy P.J. J. Steroid Biochem. Mol. Biol. 60:19-24(1997) [PubMed: 9182854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U66827 mRNA. Translation: AAB06793.1.
RefSeq	NP_032317.2.
UniGene	Mm.5109
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000033122. Mus musculus. [Contig view]
GeneID	15487.
KEGG	mmu:15487.
Organism-specific databases
MGI	MGI:107177. Hsd17b3.
Phylogenomic databases
HOGENOM	P70385.
HOVERGEN	P70385.
Gene expression databases
ArrayExpress	P70385.
CleanEx	MM_HSD17B3.
GermOnline	ENSMUSG00000033122. Mus musculus.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	288354.
SOURCE	Search...

Entry information

Entry name

DHB3_MOUSE

Accession

Primary (citable) accession number: P70385

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 25, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)