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Protein

Testosterone 17-beta-dehydrogenase 3

Gene

Hsd17b3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH.By similarity

Catalytic activityi

Testosterone + NADP+ = androst-4-ene-3,17-dione + NADPH.

Pathwayi: testosterone biosynthesis

This protein is involved in the pathway testosterone biosynthesis, which is part of Hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway testosterone biosynthesis and in Hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei181SubstrateBy similarity1
Active sitei194Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 73NADPBy similarityAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-MMU-193048. Androgen biosynthesis.
UniPathwayiUPA00367.

Names & Taxonomyi

Protein namesi
Recommended name:
Testosterone 17-beta-dehydrogenase 3 (EC:1.1.1.64)
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase type 3
Short name:
17-beta-HSD 3
Testicular 17-beta-hydroxysteroid dehydrogenase
Gene namesi
Name:Hsd17b3
Synonyms:Edh17b3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:107177. Hsd17b3.

Subcellular locationi

  • Endoplasmic reticulum By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1932905.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000545741 – 305Testosterone 17-beta-dehydrogenase 3Add BLAST305

Proteomic databases

PaxDbiP70385.
PRIDEiP70385.

PTM databases

PhosphoSitePlusiP70385.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033122.
CleanExiMM_HSD17B3.
GenevisibleiP70385. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044217.

Chemistry databases

BindingDBiP70385.

Structurei

3D structure databases

ProteinModelPortaliP70385.
SMRiP70385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1014. Eukaryota.
COG0300. LUCA.
GeneTreeiENSGT00390000010069.
HOGENOMiHOG000039237.
HOVERGENiHBG005478.
InParanoidiP70385.
KOiK10207.
OMAiLEIGILX.
OrthoDBiEOG091G06T2.
TreeFamiTF314591.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR033281. HSD17B3.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF621. PTHR24322:SF621. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PIRSFiPIRSF000126. 11-beta-HSD1. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70385-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKLFIAAGL FVGLVCLVKC MRFSQHLFLR FCKALPSSFL RSMGQWAVIT
60 70 80 90 100
GAGDGIGKAY SFELARHGLN VVLISRTLEK LQTIAEEIER TTGSCVKIVQ
110 120 130 140 150
ADFTREDIYD HIKEHLEGLE IGILVNNVGM LPSFFPSHFL STSGESQNLI
160 170 180 190 200
HCNITSVVKM TQLVLKHMES RRKGLILNIS SGAALRPWPL YSLYSASKAF
210 220 230 240 250
VYTFSKALSV EYRDKGIIIQ VLTPYSISTP MTKYLNNKMT KTADEFVKES
260 270 280 290 300
LKYVTIGAES CGCLAHEIIA IILNRIPSRI FYSSTAQRFL LTRYSDYLKR

NISNR
Length:305
Mass (Da):34,330
Last modified:October 3, 2012 - v2
Checksum:i8EA9966B2777064D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95C → G in AAB06793 (PubMed:9182854).Curated1
Sequence conflicti121I → N in AAB06793 (PubMed:9182854).Curated1
Sequence conflicti142T → S in AAB06793 (PubMed:9182854).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66827 mRNA. Translation: AAB06793.1.
CT009717 Genomic DNA. No translation available.
CH466631 Genomic DNA. Translation: EDL16224.1.
CCDSiCCDS26594.1.
RefSeqiNP_032317.2. NM_008291.3.
UniGeneiMm.5109.

Genome annotation databases

EnsembliENSMUST00000039832; ENSMUSP00000044217; ENSMUSG00000033122.
ENSMUST00000166224; ENSMUSP00000132011; ENSMUSG00000033122.
GeneIDi15487.
KEGGimmu:15487.
UCSCiuc007qyf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66827 mRNA. Translation: AAB06793.1.
CT009717 Genomic DNA. No translation available.
CH466631 Genomic DNA. Translation: EDL16224.1.
CCDSiCCDS26594.1.
RefSeqiNP_032317.2. NM_008291.3.
UniGeneiMm.5109.

3D structure databases

ProteinModelPortaliP70385.
SMRiP70385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044217.

Chemistry databases

BindingDBiP70385.
ChEMBLiCHEMBL1932905.

PTM databases

PhosphoSitePlusiP70385.

Proteomic databases

PaxDbiP70385.
PRIDEiP70385.

Protocols and materials databases

DNASUi15487.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039832; ENSMUSP00000044217; ENSMUSG00000033122.
ENSMUST00000166224; ENSMUSP00000132011; ENSMUSG00000033122.
GeneIDi15487.
KEGGimmu:15487.
UCSCiuc007qyf.1. mouse.

Organism-specific databases

CTDi3293.
MGIiMGI:107177. Hsd17b3.

Phylogenomic databases

eggNOGiKOG1014. Eukaryota.
COG0300. LUCA.
GeneTreeiENSGT00390000010069.
HOGENOMiHOG000039237.
HOVERGENiHBG005478.
InParanoidiP70385.
KOiK10207.
OMAiLEIGILX.
OrthoDBiEOG091G06T2.
TreeFamiTF314591.

Enzyme and pathway databases

UniPathwayiUPA00367.
ReactomeiR-MMU-193048. Androgen biosynthesis.

Miscellaneous databases

PROiP70385.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033122.
CleanExiMM_HSD17B3.
GenevisibleiP70385. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR033281. HSD17B3.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF621. PTHR24322:SF621. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PIRSFiPIRSF000126. 11-beta-HSD1. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHB3_MOUSE
AccessioniPrimary (citable) accession number: P70385
Secondary accession number(s): G3UWF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 3, 2012
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.