Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P70385

- DHB3_MOUSE

UniProt

P70385 - DHB3_MOUSE

Protein

Testosterone 17-beta-dehydrogenase 3

Gene

Hsd17b3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH By similarity.By similarity

    Catalytic activityi

    Testosterone + NADP+ = androst-4-ene-3,17-dione + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei181 – 1811SubstrateBy similarity
    Active sitei194 – 1941Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi44 – 7330NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. testosterone 17-beta-dehydrogenase (NADP+) activity Source: UniProtKB-EC

    GO - Biological processi

    1. testosterone biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    ReactomeiREACT_220332. Androgen biosynthesis.
    UniPathwayiUPA00367.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Testosterone 17-beta-dehydrogenase 3 (EC:1.1.1.64)
    Alternative name(s):
    17-beta-hydroxysteroid dehydrogenase type 3
    Short name:
    17-beta-HSD 3
    Testicular 17-beta-hydroxysteroid dehydrogenase
    Gene namesi
    Name:Hsd17b3
    Synonyms:Edh17b3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:107177. Hsd17b3.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 305305Testosterone 17-beta-dehydrogenase 3PRO_0000054574Add
    BLAST

    Proteomic databases

    PRIDEiP70385.

    PTM databases

    PhosphoSiteiP70385.

    Expressioni

    Gene expression databases

    CleanExiMM_HSD17B3.
    GenevestigatoriP70385.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000044217.

    Structurei

    3D structure databases

    ProteinModelPortaliP70385.
    SMRiP70385. Positions 47-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0300.
    GeneTreeiENSGT00390000010069.
    HOGENOMiHOG000039237.
    HOVERGENiHBG005478.
    InParanoidiP70385.
    KOiK10207.
    OMAiXVLTPYA.
    OrthoDBiEOG7CZK63.
    TreeFamiTF314591.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000126. 11-beta-HSD1. 1 hit.
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P70385-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKLFIAAGL FVGLVCLVKC MRFSQHLFLR FCKALPSSFL RSMGQWAVIT    50
    GAGDGIGKAY SFELARHGLN VVLISRTLEK LQTIAEEIER TTGSCVKIVQ 100
    ADFTREDIYD HIKEHLEGLE IGILVNNVGM LPSFFPSHFL STSGESQNLI 150
    HCNITSVVKM TQLVLKHMES RRKGLILNIS SGAALRPWPL YSLYSASKAF 200
    VYTFSKALSV EYRDKGIIIQ VLTPYSISTP MTKYLNNKMT KTADEFVKES 250
    LKYVTIGAES CGCLAHEIIA IILNRIPSRI FYSSTAQRFL LTRYSDYLKR 300
    NISNR 305
    Length:305
    Mass (Da):34,330
    Last modified:October 3, 2012 - v2
    Checksum:i8EA9966B2777064D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti95 – 951C → G in AAB06793. (PubMed:9182854)Curated
    Sequence conflicti121 – 1211I → N in AAB06793. (PubMed:9182854)Curated
    Sequence conflicti142 – 1421T → S in AAB06793. (PubMed:9182854)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U66827 mRNA. Translation: AAB06793.1.
    CT009717 Genomic DNA. No translation available.
    CH466631 Genomic DNA. Translation: EDL16224.1.
    CCDSiCCDS26594.1.
    RefSeqiNP_032317.2. NM_008291.3.
    UniGeneiMm.5109.

    Genome annotation databases

    EnsembliENSMUST00000039832; ENSMUSP00000044217; ENSMUSG00000033122.
    ENSMUST00000166224; ENSMUSP00000132011; ENSMUSG00000033122.
    GeneIDi15487.
    KEGGimmu:15487.
    UCSCiuc007qyf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U66827 mRNA. Translation: AAB06793.1 .
    CT009717 Genomic DNA. No translation available.
    CH466631 Genomic DNA. Translation: EDL16224.1 .
    CCDSi CCDS26594.1.
    RefSeqi NP_032317.2. NM_008291.3.
    UniGenei Mm.5109.

    3D structure databases

    ProteinModelPortali P70385.
    SMRi P70385. Positions 47-290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000044217.

    Chemistry

    BindingDBi P70385.
    ChEMBLi CHEMBL1932905.

    PTM databases

    PhosphoSitei P70385.

    Proteomic databases

    PRIDEi P70385.

    Protocols and materials databases

    DNASUi 15487.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000039832 ; ENSMUSP00000044217 ; ENSMUSG00000033122 .
    ENSMUST00000166224 ; ENSMUSP00000132011 ; ENSMUSG00000033122 .
    GeneIDi 15487.
    KEGGi mmu:15487.
    UCSCi uc007qyf.1. mouse.

    Organism-specific databases

    CTDi 3293.
    MGIi MGI:107177. Hsd17b3.

    Phylogenomic databases

    eggNOGi COG0300.
    GeneTreei ENSGT00390000010069.
    HOGENOMi HOG000039237.
    HOVERGENi HBG005478.
    InParanoidi P70385.
    KOi K10207.
    OMAi XVLTPYA.
    OrthoDBi EOG7CZK63.
    TreeFami TF314591.

    Enzyme and pathway databases

    UniPathwayi UPA00367 .
    Reactomei REACT_220332. Androgen biosynthesis.

    Miscellaneous databases

    NextBioi 288354.
    PROi P70385.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_HSD17B3.
    Genevestigatori P70385.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000126. 11-beta-HSD1. 1 hit.
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of mouse 17beta-hydroxysteroid dehydrogenase type 3 cDNA and tissue distribution of the type 1 and type 3 isoform mRNAs."
      Sha J.A., Dudley K., Rajapaksha W.R.A.K.J.S., O'Shaughnessy P.J.
      J. Steroid Biochem. Mol. Biol. 60:19-24(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 81-90, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiDHB3_MOUSE
    AccessioniPrimary (citable) accession number: P70385
    Secondary accession number(s): G3UWF9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3