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P70375

- FA7_MOUSE

UniProt

P70375 - FA7_MOUSE

Protein

Coagulation factor VII

Gene

F7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium By similarity.By similarity

    Catalytic activityi

    Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei193 – 1942Cleavage; by factor Xa, factor XIIa, factor IXa, or thrombinBy similarity
    Active sitei234 – 2341Charge relay systemBy similarity
    Active sitei283 – 2831Charge relay systemBy similarity
    Binding sitei379 – 3791SubstrateBy similarity
    Active sitei385 – 3851Charge relay systemBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. blood coagulation Source: MGI
    2. circadian rhythm Source: Ensembl
    3. organ regeneration Source: Ensembl
    4. positive regulation of blood coagulation Source: Ensembl
    5. positive regulation of leukocyte chemotaxis Source: Ensembl
    6. positive regulation of platelet-derived growth factor receptor signaling pathway Source: Ensembl
    7. positive regulation of positive chemotaxis Source: Ensembl
    8. positive regulation of protein kinase B signaling Source: Ensembl
    9. response to estrogen Source: Ensembl
    10. response to growth hormone Source: Ensembl
    11. response to vitamin K Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Blood coagulation, Hemostasis

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_115781. Bmal1:Clock,Npas2 activates circadian gene expression.
    REACT_198620. BMAL1:CLOCK,NPAS2 activates circadian gene expression.

    Protein family/group databases

    MEROPSiS01.215.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coagulation factor VII (EC:3.4.21.21)
    Alternative name(s):
    Serum prothrombin conversion accelerator
    Cleaved into the following 2 chains:
    Gene namesi
    Name:F7
    Synonyms:Cf7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:109325. F7.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome
    2. extracellular space Source: Ensembl
    3. vesicle Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 4117Sequence AnalysisPRO_0000027732Add
    BLAST
    Chaini42 – 193152Factor VII light chainPRO_0000027733Add
    BLAST
    Chaini194 – 446253Factor VII heavy chainPRO_0000027734Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei47 – 4714-carboxyglutamatePROSITE-ProRule annotation
    Modified residuei48 – 4814-carboxyglutamatePROSITE-ProRule annotation
    Modified residuei55 – 5514-carboxyglutamatePROSITE-ProRule annotation
    Modified residuei57 – 5714-carboxyglutamatePROSITE-ProRule annotation
    Disulfide bondi58 ↔ 63By similarity
    Modified residuei60 – 6014-carboxyglutamatePROSITE-ProRule annotation
    Modified residuei61 – 6114-carboxyglutamatePROSITE-ProRule annotation
    Modified residuei66 – 6614-carboxyglutamatePROSITE-ProRule annotation
    Modified residuei67 – 6714-carboxyglutamatePROSITE-ProRule annotation
    Modified residuei70 – 7014-carboxyglutamatePROSITE-ProRule annotation
    Modified residuei76 – 7614-carboxyglutamatePROSITE-ProRule annotation
    Disulfide bondi91 ↔ 102By similarity
    Glycosylationi93 – 931O-linked (Glc...); alternateBy similarity
    Glycosylationi93 – 931O-linked (Xyl...); alternateBy similarity
    Disulfide bondi96 ↔ 111By similarity
    Modified residuei104 – 1041(3R)-3-hydroxyaspartateBy similarity
    Disulfide bondi113 ↔ 122By similarity
    Disulfide bondi132 ↔ 143By similarity
    Disulfide bondi139 ↔ 153By similarity
    Disulfide bondi155 ↔ 168By similarity
    Disulfide bondi176 ↔ 303By similarity
    Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi200 ↔ 205By similarity
    Disulfide bondi219 ↔ 235By similarity
    Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi351 ↔ 370By similarity
    Disulfide bondi381 ↔ 409By similarity

    Post-translational modificationi

    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.By similarity
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity
    Can be either O-glucosylated or O-xylosylated at Ser-93 by POGLUT1.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Gamma-carboxyglutamic acid, Glycoprotein, Hydroxylation, Zymogen

    Proteomic databases

    PaxDbiP70375.
    PRIDEiP70375.

    Expressioni

    Tissue specificityi

    Plasma and liver.

    Inductioni

    Expression in the liver and plasma oscillates in a circadian manner.1 Publication

    Gene expression databases

    ArrayExpressiP70375.
    BgeeiP70375.
    CleanExiMM_F7.
    GenevestigatoriP70375.

    Interactioni

    Subunit structurei

    Heterodimer of a light chain and a heavy chain linked by a disulfide bond.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP70375.
    SMRiP70375. Positions 49-184, 194-445.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 8645GlaPROSITE-ProRule annotationAdd
    BLAST
    Domaini87 – 12337EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini128 – 16942EGF-like 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini194 – 433240Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 2 EGF-like domains.PROSITE-ProRule annotation
    Contains 1 Gla (gamma-carboxy-glutamate) domain.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251821.
    HOVERGENiHBG013304.
    InParanoidiP70375.
    KOiK01320.
    OMAiGCEQYCS.
    OrthoDBiEOG75B84T.
    PhylomeDBiP70375.
    TreeFamiTF327329.

    Family and domain databases

    Gene3Di4.10.740.10. 1 hit.
    InterProiIPR017857. Coagulation_fac_subgr_Gla_dom.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR000294. GLA_domain.
    IPR012224. Pept_S1A_FX.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00008. EGF. 1 hit.
    PF00594. Gla. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001143. Factor_X. 1 hit.
    PRINTSiPR00722. CHYMOTRYPSIN.
    PR00001. GLABLOOD.
    SMARTiSM00181. EGF. 1 hit.
    SM00179. EGF_CA. 1 hit.
    SM00069. GLA. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF57630. SSF57630. 1 hit.
    PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
    PS00022. EGF_1. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS01187. EGF_CA. 1 hit.
    PS00011. GLA_1. 1 hit.
    PS50998. GLA_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P70375-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVPQAHGLLL LCFLLQLQGP LGTAVFITQE EAHGVLHRQR RANSLLEELW    50
    PGSLERECNE EQCSFEEARE IFKSPERTKQ FWIVYSDGDQ CASNPCQNGG 100
    TCQDHLKSYV CFCLLDFEGR NCEKSKNEQL ICANENGDCD QYCRDHVGTK 150
    RTCSCHEDYT LQPDEVSCKP KVEYPCGRIP VVEKRNSSSR QGRIVGGNVC 200
    PKGECPWQAV LKINGLLLCG AVLLDARWIV TAAHCFDNIR YWGNITVVMG 250
    EHDFSEKDGD EQVRRVTQVI MPDKYIRGKI NHDIALLRLH RPVTFTDYVV 300
    PLCLPEKSFS ENTLARIRFS RVSGWGQLLD RGATALELMS IEVPRLMTQD 350
    CLEHAKHSSN TPKITENMFC AGYMDGTKDA CKGDSGGPHA THYHGTWYLT 400
    GVVSWGEGCA AIGHIGVYTR VSQYIDWLVR HMDSKLQVGV FRLPLL 446
    Length:446
    Mass (Da):50,276
    Last modified:February 1, 1997 - v1
    Checksum:i2512E44A45CBC96E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti99 – 991G → V in AAC52570. (PubMed:8972017)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U44795 mRNA. Translation: AAC52570.1.
    U66079 Genomic DNA. Translation: AAC33796.1.
    BC061149 mRNA. Translation: AAH61149.1.
    CCDSiCCDS22104.1.
    RefSeqiNP_034302.2. NM_010172.3.
    UniGeneiMm.4827.

    Genome annotation databases

    EnsembliENSMUST00000033820; ENSMUSP00000033820; ENSMUSG00000031443.
    GeneIDi14068.
    KEGGimmu:14068.
    UCSCiuc009kwr.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U44795 mRNA. Translation: AAC52570.1 .
    U66079 Genomic DNA. Translation: AAC33796.1 .
    BC061149 mRNA. Translation: AAH61149.1 .
    CCDSi CCDS22104.1.
    RefSeqi NP_034302.2. NM_010172.3.
    UniGenei Mm.4827.

    3D structure databases

    ProteinModelPortali P70375.
    SMRi P70375. Positions 49-184, 194-445.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.215.

    Proteomic databases

    PaxDbi P70375.
    PRIDEi P70375.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033820 ; ENSMUSP00000033820 ; ENSMUSG00000031443 .
    GeneIDi 14068.
    KEGGi mmu:14068.
    UCSCi uc009kwr.2. mouse.

    Organism-specific databases

    CTDi 2155.
    MGIi MGI:109325. F7.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251821.
    HOVERGENi HBG013304.
    InParanoidi P70375.
    KOi K01320.
    OMAi GCEQYCS.
    OrthoDBi EOG75B84T.
    PhylomeDBi P70375.
    TreeFami TF327329.

    Enzyme and pathway databases

    Reactomei REACT_115781. Bmal1:Clock,Npas2 activates circadian gene expression.
    REACT_198620. BMAL1:CLOCK,NPAS2 activates circadian gene expression.

    Miscellaneous databases

    NextBioi 285056.
    PROi P70375.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70375.
    Bgeei P70375.
    CleanExi MM_F7.
    Genevestigatori P70375.

    Family and domain databases

    Gene3Di 4.10.740.10. 1 hit.
    InterProi IPR017857. Coagulation_fac_subgr_Gla_dom.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR000294. GLA_domain.
    IPR012224. Pept_S1A_FX.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00008. EGF. 1 hit.
    PF00594. Gla. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001143. Factor_X. 1 hit.
    PRINTSi PR00722. CHYMOTRYPSIN.
    PR00001. GLABLOOD.
    SMARTi SM00181. EGF. 1 hit.
    SM00179. EGF_CA. 1 hit.
    SM00069. GLA. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF57630. SSF57630. 1 hit.
    PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
    PS00022. EGF_1. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS01187. EGF_CA. 1 hit.
    PS00011. GLA_1. 1 hit.
    PS50998. GLA_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a cDNA encoding murine coagulation factor VII."
      Idusogie E., Rosen E., Geng J.P., Carmeliet P., Collen D., Castellino F.J.
      Thromb. Haemost. 75:481-487(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Nucleotide structure and characterization of the murine blood coagulation factor VII gene."
      Idusogie E., Rosen E.D., Carmeliet P., Collen D., Castellino F.J.
      Thromb. Haemost. 76:957-964(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    4. "Evidence for an overlapping role of CLOCK and NPAS2 transcription factors in liver circadian oscillators."
      Bertolucci C., Cavallari N., Colognesi I., Aguzzi J., Chen Z., Caruso P., Foa A., Tosini G., Bernardi F., Pinotti M.
      Mol. Cell. Biol. 28:3070-3075(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiFA7_MOUSE
    AccessioniPrimary (citable) accession number: P70375
    Secondary accession number(s): Q61109
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 149 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3