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Protein

Coagulation factor VII

Gene

F7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium (By similarity).By similarity

Catalytic activityi

Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei193 – 1942Cleavage; by factor Xa, factor XIIa, factor IXa, or thrombinBy similarity
Active sitei234 – 2341Charge relay systemBy similarity
Active sitei283 – 2831Charge relay systemBy similarity
Binding sitei379 – 3791SubstrateBy similarity
Active sitei385 – 3851Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Blood coagulation, Hemostasis

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_115781. Bmal1:Clock,Npas2 activates circadian gene expression.
REACT_281620. Gamma-carboxylation of protein precursors.
REACT_325479. Extrinsic Pathway of Fibrin Clot Formation.
REACT_344843. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
REACT_351509. Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus.
REACT_352087. Removal of aminoterminal propeptides from gamma-carboxylated proteins.

Protein family/group databases

MEROPSiS01.215.

Names & Taxonomyi

Protein namesi
Recommended name:
Coagulation factor VII (EC:3.4.21.21)
Alternative name(s):
Serum prothrombin conversion accelerator
Cleaved into the following 2 chains:
Gene namesi
Name:F7
Synonyms:Cf7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:109325. F7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Propeptidei25 – 4117Sequence AnalysisPRO_0000027732Add
BLAST
Chaini42 – 193152Factor VII light chainPRO_0000027733Add
BLAST
Chaini194 – 446253Factor VII heavy chainPRO_0000027734Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei47 – 4714-carboxyglutamatePROSITE-ProRule annotation
Modified residuei48 – 4814-carboxyglutamatePROSITE-ProRule annotation
Modified residuei55 – 5514-carboxyglutamatePROSITE-ProRule annotation
Modified residuei57 – 5714-carboxyglutamatePROSITE-ProRule annotation
Disulfide bondi58 ↔ 63By similarity
Modified residuei60 – 6014-carboxyglutamatePROSITE-ProRule annotation
Modified residuei61 – 6114-carboxyglutamatePROSITE-ProRule annotation
Modified residuei66 – 6614-carboxyglutamatePROSITE-ProRule annotation
Modified residuei67 – 6714-carboxyglutamatePROSITE-ProRule annotation
Modified residuei70 – 7014-carboxyglutamatePROSITE-ProRule annotation
Modified residuei76 – 7614-carboxyglutamatePROSITE-ProRule annotation
Disulfide bondi91 ↔ 102By similarity
Glycosylationi93 – 931O-linked (Glc...); alternateBy similarity
Glycosylationi93 – 931O-linked (Xyl...); alternateBy similarity
Disulfide bondi96 ↔ 111By similarity
Modified residuei104 – 1041(3R)-3-hydroxyaspartateBy similarity
Disulfide bondi113 ↔ 122By similarity
Disulfide bondi132 ↔ 143By similarity
Disulfide bondi139 ↔ 153By similarity
Disulfide bondi155 ↔ 168By similarity
Disulfide bondi176 ↔ 303By similarity
Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi200 ↔ 205By similarity
Disulfide bondi219 ↔ 235By similarity
Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi351 ↔ 370By similarity
Disulfide bondi381 ↔ 409By similarity

Post-translational modificationi

The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.By similarity
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity
Can be either O-glucosylated or O-xylosylated at Ser-93 by POGLUT1.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Gamma-carboxyglutamic acid, Glycoprotein, Hydroxylation, Zymogen

Proteomic databases

MaxQBiP70375.
PaxDbiP70375.
PRIDEiP70375.

Expressioni

Tissue specificityi

Plasma and liver.

Inductioni

Expression in the liver and plasma oscillates in a circadian manner.1 Publication

Gene expression databases

BgeeiP70375.
CleanExiMM_F7.
ExpressionAtlasiP70375. baseline and differential.
GenevisibleiP70375. MM.

Interactioni

Subunit structurei

Heterodimer of a light chain and a heavy chain linked by a disulfide bond.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033820.

Structurei

3D structure databases

ProteinModelPortaliP70375.
SMRiP70375. Positions 49-184, 194-445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 8645GlaPROSITE-ProRule annotationAdd
BLAST
Domaini87 – 12337EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini128 – 16942EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini194 – 433240Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 Gla (gamma-carboxy-glutamate) domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251821.
HOVERGENiHBG013304.
InParanoidiP70375.
KOiK01320.
OMAiGCEQYCS.
OrthoDBiEOG75B84T.
PhylomeDBiP70375.
TreeFamiTF327329.

Family and domain databases

Gene3Di4.10.740.10. 1 hit.
InterProiIPR017857. Coagulation_fac_subgr_Gla_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR000294. GLA_domain.
IPR012224. Pept_S1A_FX.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF00594. Gla. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001143. Factor_X. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
PR00001. GLABLOOD.
SMARTiSM00181. EGF. 1 hit.
SM00179. EGF_CA. 1 hit.
SM00069. GLA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57630. SSF57630. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00011. GLA_1. 1 hit.
PS50998. GLA_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70375-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPQAHGLLL LCFLLQLQGP LGTAVFITQE EAHGVLHRQR RANSLLEELW
60 70 80 90 100
PGSLERECNE EQCSFEEARE IFKSPERTKQ FWIVYSDGDQ CASNPCQNGG
110 120 130 140 150
TCQDHLKSYV CFCLLDFEGR NCEKSKNEQL ICANENGDCD QYCRDHVGTK
160 170 180 190 200
RTCSCHEDYT LQPDEVSCKP KVEYPCGRIP VVEKRNSSSR QGRIVGGNVC
210 220 230 240 250
PKGECPWQAV LKINGLLLCG AVLLDARWIV TAAHCFDNIR YWGNITVVMG
260 270 280 290 300
EHDFSEKDGD EQVRRVTQVI MPDKYIRGKI NHDIALLRLH RPVTFTDYVV
310 320 330 340 350
PLCLPEKSFS ENTLARIRFS RVSGWGQLLD RGATALELMS IEVPRLMTQD
360 370 380 390 400
CLEHAKHSSN TPKITENMFC AGYMDGTKDA CKGDSGGPHA THYHGTWYLT
410 420 430 440
GVVSWGEGCA AIGHIGVYTR VSQYIDWLVR HMDSKLQVGV FRLPLL
Length:446
Mass (Da):50,276
Last modified:February 1, 1997 - v1
Checksum:i2512E44A45CBC96E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti99 – 991G → V in AAC52570 (PubMed:8972017).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44795 mRNA. Translation: AAC52570.1.
U66079 Genomic DNA. Translation: AAC33796.1.
BC061149 mRNA. Translation: AAH61149.1.
CCDSiCCDS22104.1.
RefSeqiNP_034302.2. NM_010172.3.
UniGeneiMm.4827.

Genome annotation databases

EnsembliENSMUST00000033820; ENSMUSP00000033820; ENSMUSG00000031443.
GeneIDi14068.
KEGGimmu:14068.
UCSCiuc009kwr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44795 mRNA. Translation: AAC52570.1.
U66079 Genomic DNA. Translation: AAC33796.1.
BC061149 mRNA. Translation: AAH61149.1.
CCDSiCCDS22104.1.
RefSeqiNP_034302.2. NM_010172.3.
UniGeneiMm.4827.

3D structure databases

ProteinModelPortaliP70375.
SMRiP70375. Positions 49-184, 194-445.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033820.

Protein family/group databases

MEROPSiS01.215.

Proteomic databases

MaxQBiP70375.
PaxDbiP70375.
PRIDEiP70375.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033820; ENSMUSP00000033820; ENSMUSG00000031443.
GeneIDi14068.
KEGGimmu:14068.
UCSCiuc009kwr.2. mouse.

Organism-specific databases

CTDi2155.
MGIiMGI:109325. F7.

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251821.
HOVERGENiHBG013304.
InParanoidiP70375.
KOiK01320.
OMAiGCEQYCS.
OrthoDBiEOG75B84T.
PhylomeDBiP70375.
TreeFamiTF327329.

Enzyme and pathway databases

ReactomeiREACT_115781. Bmal1:Clock,Npas2 activates circadian gene expression.
REACT_281620. Gamma-carboxylation of protein precursors.
REACT_325479. Extrinsic Pathway of Fibrin Clot Formation.
REACT_344843. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
REACT_351509. Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus.
REACT_352087. Removal of aminoterminal propeptides from gamma-carboxylated proteins.

Miscellaneous databases

NextBioi285056.
PROiP70375.
SOURCEiSearch...

Gene expression databases

BgeeiP70375.
CleanExiMM_F7.
ExpressionAtlasiP70375. baseline and differential.
GenevisibleiP70375. MM.

Family and domain databases

Gene3Di4.10.740.10. 1 hit.
InterProiIPR017857. Coagulation_fac_subgr_Gla_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR000294. GLA_domain.
IPR012224. Pept_S1A_FX.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF00594. Gla. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001143. Factor_X. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
PR00001. GLABLOOD.
SMARTiSM00181. EGF. 1 hit.
SM00179. EGF_CA. 1 hit.
SM00069. GLA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57630. SSF57630. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00011. GLA_1. 1 hit.
PS50998. GLA_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a cDNA encoding murine coagulation factor VII."
    Idusogie E., Rosen E., Geng J.P., Carmeliet P., Collen D., Castellino F.J.
    Thromb. Haemost. 75:481-487(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Nucleotide structure and characterization of the murine blood coagulation factor VII gene."
    Idusogie E., Rosen E.D., Carmeliet P., Collen D., Castellino F.J.
    Thromb. Haemost. 76:957-964(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  4. "Evidence for an overlapping role of CLOCK and NPAS2 transcription factors in liver circadian oscillators."
    Bertolucci C., Cavallari N., Colognesi I., Aguzzi J., Chen Z., Caruso P., Foa A., Tosini G., Bernardi F., Pinotti M.
    Mol. Cell. Biol. 28:3070-3075(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiFA7_MOUSE
AccessioniPrimary (citable) accession number: P70375
Secondary accession number(s): Q61109
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 24, 2015
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.