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P70372 (ELAV1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ELAV-like protein 1
Alternative name(s):
Elav-like generic protein
Hu-antigen R
Short name=HuR
MelG
Gene names
Name:Elavl1
Synonyms:Elra, Hua
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA. Ref.6 Ref.7

Subunit structure

Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A By similarity. Interact with ZNF385A; the interaction is indirect and mRNA-dependent and may regulate p53/TP53 expression. Interacts with IGF2BP2 and IGF2BP3 By similarity. Ref.6

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Translocates into the cytoplasm following phosphorylation by MAPKAPK2 By similarity.

Post-translational modification

Phosphorylated by MAPKAPK2 By similarity.

Methylated at Arg-217 by CARM1 in T-cells in response to LPS challenge. Ref.5

Sequence similarities

Belongs to the RRM elav family.

Contains 3 RRM (RNA recognition motif) domains.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 326325ELAV-like protein 1
PRO_0000081578

Regions

Domain20 – 9879RRM 1
Domain106 – 18681RRM 2
Domain244 – 32279RRM 3

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue21Phosphoserine By similarity
Modified residue2021Phosphoserine By similarity
Modified residue2171Omega-N-methylated arginine; by CARM1 Ref.5

Experimental info

Sequence conflict31N → G in AAB17967. Ref.1
Sequence conflict151D → G in AAB17967. Ref.1
Sequence conflict1681N → I in AAB17967. Ref.1
Sequence conflict1681N → I in AAA96941. Ref.4
Sequence conflict2981A → S in AAB17967. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P70372 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 74A98DEE5B921AF6

FASTA32636,169
        10         20         30         40         50         60 
MSNGYEDHMA EDCRDDIGRT NLIVNYLPQN MTQEELRSLF SSIGEVESAK LIRDKVAGHS 

        70         80         90        100        110        120 
LGYGFVNYVT AKDAERAIST LNGLRLQSKT IKVSYARPSS EVIKDANLYI SGLPRTMTQK 

       130        140        150        160        170        180 
DVEDMFSRFG RIINSRVLVD QTTGLSRGVA FIRFDKRSEA EEAITSFNGH KPPGSSEPIT 

       190        200        210        220        230        240 
VKFAANPNQN KNMALLSQLY HSPARRFGGP VHHQAQRFRF SPMGVDHMSG ISGVNVPGNA 

       250        260        270        280        290        300 
SSGWCIFIYN LGQDADEGIL WQMFGPFGAV TNVKVIRDFN TNKCKGFGFV TMTNYEEAAM 

       310        320 
AIASLNGYRL GDKILQVSFK TNKSHK 

« Hide

References

« Hide 'large scale' references
[1]"ELAV protein HuA (HuR) can redistribute between nucleus and cytoplasm and is upregulated during serum stimulation and T cell activation."
Atasoy U., Watson J., Patel D., Keene J.D.
J. Cell Sci. 111:3145-3156(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain and Spleen.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Thymus.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"A conserved family of elav-like genes in vertebrates."
Good P.J.
Proc. Natl. Acad. Sci. U.S.A. 92:4557-4561(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 119-292.
Tissue: Brain.
[5]"Lipopolysaccharide-induced methylation of HuR, an mRNA-stabilizing protein, by CARM1. Coactivator-associated arginine methyltransferase."
Li H., Park S., Kilburn B., Jelinek M.A., Henschen-Edman A., Aswad D.W., Stallcup M.R., Laird-Offringa I.A.
J. Biol. Chem. 277:44623-44630(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT ARG-217.
[6]"Cooperative role of the RNA-binding proteins Hzf and HuR in p53 activation."
Nakamura H., Kawagishi H., Watanabe A., Sugimoto K., Maruyama M., Sugimoto M.
Mol. Cell. Biol. 31:1997-2009(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN MRNA LOCALIZATION, INTERACTION WITH ZNF385A, RNA-BINDING.
[7]"N-methyladenosine modification destabilizes developmental regulators in embryonic stem cells."
Wang Y., Li Y., Toth J.I., Petroski M.D., Zhang Z., Zhao J.C.
Nat. Cell Biol. 16:191-198(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, RNA-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U65735 mRNA. Translation: AAB17967.1.
AK080365 mRNA. Translation: BAC37892.1.
CH466566 Genomic DNA. Translation: EDL21996.1.
CH466566 Genomic DNA. Translation: EDL21997.1.
U17595 mRNA. Translation: AAA96941.1.
PIRI49144.
RefSeqNP_034615.2. NM_010485.3.
XP_006508761.1. XM_006508698.1.
UniGeneMm.119162.

3D structure databases

ProteinModelPortalP70372.
SMRP70372. Positions 18-324.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200482. 3 interactions.
DIPDIP-46480N.
IntActP70372. 7 interactions.
MINTMINT-4094207.
STRING10090.ENSMUSP00000096549.

PTM databases

PhosphoSiteP70372.

Proteomic databases

PaxDbP70372.
PRIDEP70372.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000098950; ENSMUSP00000096549; ENSMUSG00000040028.
GeneID15568.
KEGGmmu:15568.
UCSCuc009kts.1. mouse.

Organism-specific databases

CTD1994.
MGIMGI:1100851. Elavl1.

Phylogenomic databases

eggNOGCOG0724.
GeneTreeENSGT00560000077064.
HOGENOMHOG000231162.
HOVERGENHBG002295.
InParanoidP70372.
KOK13088.
OMARFGGPLH.
OrthoDBEOG77T14R.
TreeFamTF313377.

Gene expression databases

ArrayExpressP70372.
BgeeP70372.
GenevestigatorP70372.

Family and domain databases

Gene3D3.30.70.330. 3 hits.
InterProIPR006548. ELAD_HUD_SF.
IPR002343. Hud_Sxl_RNA.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00076. RRM_1. 3 hits.
[Graphical view]
PRINTSPR00961. HUDSXLRNA.
SMARTSM00360. RRM. 3 hits.
[Graphical view]
TIGRFAMsTIGR01661. ELAV_HUD_SF. 1 hit.
PROSITEPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSELAVL1. mouse.
NextBio288542.
PROP70372.
SOURCESearch...

Entry information

Entry nameELAV1_MOUSE
AccessionPrimary (citable) accession number: P70372
Secondary accession number(s): Q60745, Q78QY3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot