P70372 (ELAV1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ELAV-like protein 1

Alternative name(s):
Elav-like generic protein
Hu-antigen R
Short name=HuR
MelG

Gene names

Name:	Elavl1
Synonyms:	Elra, Hua

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro By similarity.
Subunit structure	Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Translocates into the cytoplasm following phosphorylation by MAPKAPK2 By similarity.
Post-translational modification	Phosphorylated by MAPKAPK2 By similarity. Methylated at Arg-217 by CARM1 in T-cells in response to LPS challenge. Ref.5
Sequence similarities	Belongs to the RRM elav family. Contains 3 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Ligand	RNA-binding
PTM	Acetylation Methylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	3'-UTR-mediated mRNA stabilization Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of translation Inferred from mutant phenotype PubMed 21613615. Source: MGI
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from direct assay PubMed 16168373. Source: MGI
Molecular_function	mRNA 3'-UTR AU-rich region binding Inferred from sequence orthology PubMed 23056314. Source: MGI nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 326	325	ELAV-like protein 1		PRO_0000081578
Regions
	Domain	20 – 98	79	RRM 1
	Domain	106 – 186	81	RRM 2
	Domain	244 – 322	79	RRM 3
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	2	1	Phosphoserine By similarity
	Modified residue	202	1	Phosphoserine By similarity
	Modified residue	217	1	Omega-N-methylated arginine; by CARM1 Ref.5
Experimental info
	Sequence conflict	3	1	N → G in AAB17967. Ref.1
	Sequence conflict	15	1	D → G in AAB17967. Ref.1
	Sequence conflict	168	1	N → I in AAB17967. Ref.1
	Sequence conflict	168	1	N → I in AAA96941. Ref.4
	Sequence conflict	298	1	A → S in AAB17967. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P70372 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 74A98DEE5B921AF6	FASTA	326	36,169
	10 20 30 40 50 60 MSNGYEDHMA EDCRDDIGRT NLIVNYLPQN MTQEELRSLF SSIGEVESAK LIRDKVAGHS 70 80 90 100 110 120 LGYGFVNYVT AKDAERAIST LNGLRLQSKT IKVSYARPSS EVIKDANLYI SGLPRTMTQK 130 140 150 160 170 180 DVEDMFSRFG RIINSRVLVD QTTGLSRGVA FIRFDKRSEA EEAITSFNGH KPPGSSEPIT 190 200 210 220 230 240 VKFAANPNQN KNMALLSQLY HSPARRFGGP VHHQAQRFRF SPMGVDHMSG ISGVNVPGNA 250 260 270 280 290 300 SSGWCIFIYN LGQDADEGIL WQMFGPFGAV TNVKVIRDFN TNKCKGFGFV TMTNYEEAAM 310 320 AIASLNGYRL GDKILQVSFK TNKSHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ELAV protein HuA (HuR) can redistribute between nucleus and cytoplasm and is upregulated during serum stimulation and T cell activation." Atasoy U., Watson J., Patel D., Keene J.D. J. Cell Sci. 111:3145-3156(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain and Spleen.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Thymus.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"A conserved family of elav-like genes in vertebrates." Good P.J. Proc. Natl. Acad. Sci. U.S.A. 92:4557-4561(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 119-292. Tissue: Brain.
[5]	"Lipopolysaccharide-induced methylation of HuR, an mRNA-stabilizing protein, by CARM1. Coactivator-associated arginine methyltransferase." Li H., Park S., Kilburn B., Jelinek M.A., Henschen-Edman A., Aswad D.W., Stallcup M.R., Laird-Offringa I.A. J. Biol. Chem. 277:44623-44630(2002) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT ARG-217.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U65735 mRNA. Translation: AAB17967.1. AK080365 mRNA. Translation: BAC37892.1. CH466566 Genomic DNA. Translation: EDL21996.1. CH466566 Genomic DNA. Translation: EDL21997.1. U17595 mRNA. Translation: AAA96941.1.
IPI	IPI00108271.
PIR	I49144.
RefSeq	NP_034615.2. NM_010485.3.
UniGene	Mm.119162.
3D structure databases
ProteinModelPortal	P70372.
SMR	P70372. Positions 18-326.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46480N.
MINT	MINT-4094207.
STRING	10090.ENSMUSP00000096549.
PTM databases
PhosphoSite	P70372.
Proteomic databases
PaxDb	P70372.
PRIDE	P70372.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000098950; ENSMUSP00000096549; ENSMUSG00000040028.
GeneID	15568.
KEGG	mmu:15568.
UCSC	uc009kts.1. mouse.
Organism-specific databases
CTD	1994.
MGI	MGI:1100851. Elavl1.
Phylogenomic databases
eggNOG	COG0724.
GeneTree	ENSGT00560000077064.
HOGENOM	HOG000231162.
HOVERGEN	HBG002295.
InParanoid	P70372.
KO	K13088.
OMA	YANRACA.
OrthoDB	EOG4Z8XWZ.
Gene expression databases
ArrayExpress	P70372.
Bgee	P70372.
Genevestigator	P70372.
GermOnline	ENSMUSG00000040028. Mus musculus.
Family and domain databases
Gene3D	3.30.70.330. 3 hits.
InterPro	IPR006548. ELAD_HUD_SF. IPR002343. Hud_Sxl_RNA. IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00076. RRM_1. 3 hits. [Graphical view]
PRINTS	PR00961. HUDSXLRNA.
SMART	SM00360. RRM. 3 hits. [Graphical view]
TIGRFAMs	TIGR01661. ELAV_HUD_SF. 1 hit.
PROSITE	PS50102. RRM. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ELAVL1. mouse.
NextBio	288542.
SOURCE	Search...

Entry information

Entry name

ELAV1_MOUSE

Accession

Primary (citable) accession number: P70372
Secondary accession number(s): Q60745, Q78QY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	July 27, 2011
Last modified:	May 29, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program