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Protein

Histidine triad nucleotide-binding protein 1

Gene

Hint1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991Purine nucleotide phosphoramidateBy similarity
Active sitei112 – 1121Tele-AMP-histidine intermediateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 442Purine nucleotide phosphoramidateBy similarity
Nucleotide bindingi105 – 1073Purine nucleotide phosphoramidateBy similarity
Nucleotide bindingi112 – 1143Purine nucleotide phosphoramidateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine triad nucleotide-binding protein 1 (EC:3.-.-.-)
Alternative name(s):
Adenosine 5'-monophosphoramidase
Protein kinase C inhibitor 1
Protein kinase C-interacting protein 1
Short name:
PKCI-1
Gene namesi
Name:Hint1
Synonyms:Hint, Pkci, Pkci1, Prkcnh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1321133. Hint1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • histone deacetylase complex Source: UniProtKB
  • nucleus Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice display increased susceptibility to carcinogens.1 Publication

Chemistry

ChEMBLiCHEMBL1250364.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 126125Histidine triad nucleotide-binding protein 1PRO_0000109782Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei21 – 211N6-acetyllysineCombined sources
Modified residuei30 – 301N6-acetyllysineBy similarity
Modified residuei45 – 451PhosphoserineCombined sources
Modified residuei72 – 721PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP70349.
MaxQBiP70349.
PaxDbiP70349.
PeptideAtlasiP70349.
PRIDEiP70349.

2D gel databases

REPRODUCTION-2DPAGEP70349.

PTM databases

iPTMnetiP70349.
PhosphoSiteiP70349.
SwissPalmiP70349.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020267.
CleanExiMM_HINT1.
ExpressionAtlasiP70349. baseline and differential.
GenevisibleiP70349. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with CDK7. Interacts with RUVBL1 and RUVBL2 and is associated with the LEF1/TCF1-CTNNB1 complex and with a KAT5 histone acetyltransferase complex. Identified in a complex with MITF and CTNNB1. Interacts with CDC34 and RBX1, and is part of a SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi200305. 1 interaction.
IntActiP70349. 4 interactions.
MINTiMINT-1868587.
STRINGi10090.ENSMUSP00000020504.

Structurei

3D structure databases

ProteinModelPortaliP70349.
SMRiP70349. Positions 13-126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 126109HITPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi110 – 1145Histidine triad motif

Sequence similaritiesi

Belongs to the HINT family.Curated
Contains 1 HIT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3275. Eukaryota.
COG0537. LUCA.
GeneTreeiENSGT00510000046448.
HOGENOMiHOG000061064.
HOVERGENiHBG051906.
InParanoidiP70349.
KOiK02503.
OMAiYRLITNN.
OrthoDBiEOG091G1001.
PhylomeDBiP70349.
TreeFamiTF314862.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiIPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
[Graphical view]
PANTHERiPTHR23089. PTHR23089. 1 hit.
PfamiPF01230. HIT. 1 hit.
[Graphical view]
PRINTSiPR00332. HISTRIAD.
SUPFAMiSSF54197. SSF54197. 1 hit.
PROSITEiPS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70349-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADEIAKAQV AQPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT
60 70 80 90 100
HFLVIPKKHI SQISVADDDD ESLLGHLMIV GKKCAADLGL KRGYRMVVNE
110 120
GADGGQSVYH IHLHVLGGRQ MNWPPG
Length:126
Mass (Da):13,777
Last modified:January 23, 2007 - v3
Checksum:i30DA241476389805
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60001 mRNA. Translation: AAC71076.1.
AK002965 mRNA. Translation: BAB22484.1.
AK012433 mRNA. Translation: BAB28235.1.
BC070415 mRNA. Translation: AAH70415.1.
BC080296 mRNA. Translation: AAH80296.1.
CCDSiCCDS24702.1.
PIRiPN0044.
RefSeqiNP_032274.1. NM_008248.2.
UniGeneiMm.425.

Genome annotation databases

EnsembliENSMUST00000020504; ENSMUSP00000020504; ENSMUSG00000020267.
GeneIDi15254.
KEGGimmu:15254.
UCSCiuc007iyj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60001 mRNA. Translation: AAC71076.1.
AK002965 mRNA. Translation: BAB22484.1.
AK012433 mRNA. Translation: BAB28235.1.
BC070415 mRNA. Translation: AAH70415.1.
BC080296 mRNA. Translation: AAH80296.1.
CCDSiCCDS24702.1.
PIRiPN0044.
RefSeqiNP_032274.1. NM_008248.2.
UniGeneiMm.425.

3D structure databases

ProteinModelPortaliP70349.
SMRiP70349. Positions 13-126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200305. 1 interaction.
IntActiP70349. 4 interactions.
MINTiMINT-1868587.
STRINGi10090.ENSMUSP00000020504.

Chemistry

ChEMBLiCHEMBL1250364.

PTM databases

iPTMnetiP70349.
PhosphoSiteiP70349.
SwissPalmiP70349.

2D gel databases

REPRODUCTION-2DPAGEP70349.

Proteomic databases

EPDiP70349.
MaxQBiP70349.
PaxDbiP70349.
PeptideAtlasiP70349.
PRIDEiP70349.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020504; ENSMUSP00000020504; ENSMUSG00000020267.
GeneIDi15254.
KEGGimmu:15254.
UCSCiuc007iyj.1. mouse.

Organism-specific databases

CTDi3094.
MGIiMGI:1321133. Hint1.

Phylogenomic databases

eggNOGiKOG3275. Eukaryota.
COG0537. LUCA.
GeneTreeiENSGT00510000046448.
HOGENOMiHOG000061064.
HOVERGENiHBG051906.
InParanoidiP70349.
KOiK02503.
OMAiYRLITNN.
OrthoDBiEOG091G1001.
PhylomeDBiP70349.
TreeFamiTF314862.

Miscellaneous databases

PROiP70349.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020267.
CleanExiMM_HINT1.
ExpressionAtlasiP70349. baseline and differential.
GenevisibleiP70349. MM.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiIPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
[Graphical view]
PANTHERiPTHR23089. PTHR23089. 1 hit.
PfamiPF01230. HIT. 1 hit.
[Graphical view]
PRINTSiPR00332. HISTRIAD.
SUPFAMiSSF54197. SSF54197. 1 hit.
PROSITEiPS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHINT1_MOUSE
AccessioniPrimary (citable) accession number: P70349
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a protein kinase C inhibitor and to bind zinc in solution. Both seem to be incorrect.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.